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The map label for this gene is mjaIIIM [H]

Identifier: 189184374

GI number: 189184374

Start: 1498887

End: 1499696

Strand: Reverse

Name: mjaIIIM [H]

Synonym: OTT_1467

Alternate gene names: 189184374

Gene position: 1499696-1498887 (Counterclockwise)

Preceding gene: 189184378

Following gene: 189184361

Centisome position: 74.65

GC content: 29.75

Gene sequence:

>810_bases
GTGTCAGTAGCTGTTGCTAATAAGTCTAAGCCTTTCCTTCATTGGATTGGTAGTAAACGAAGAATTGTTAATAAATTAAT
AGAACATCTTCCTCAAGGTCCACACTATAATTACTATGAACCATTCCTTGGTGGAGGTGCTTTATTTTTTCAAGTTAGGC
ATCTTTTTAAACAATGTTTTTTGTCTGACATCAATCTTGATTTAATTACTAGCTATAATGCTGTTAAAAATAATCCTAAT
GAGGTCAATAGATTACTAAGTTTATATCACAAACATCATTCGAAGGATTATTATTATAAAGTTAAAAACAAATATAGTAA
TAATCCGAATGAGATTACCGCAAAATTTATATATCTTAATAAATACTCTTTTAGGGGAATTTATAGAGTCTACAAAAATG
GACAATCTGCTCAAACATTTTCTGGTGAATGCTACATTAAACTCCACATTGCATCTAGAATAAACCAATGCAGCAGCCTT
CTACATGGTGTATCAATTTATGCTACAGATTTTTCATTTATAGAGCCTCAACAAAATGACTTCGTTTATTTTGATCCTCC
TTACCATAAATCTGGAGAAAGGTTCTATACTAGACTTCCATTTGATGAAAAAGATCAAATTAGATTACGGGATTTTGTCC
AAGAATTAACAAATAAAGGTGTTAAGATTATGATCTCAAATAATAATACTGCCTTTATTAGAGACTTATACAAAGATTTT
AATATCAATACCGTTACAGTCATATACTCAATCAATGAACAACGCAATCCTGTTAATGAGCTAATCATTACTAACTATAA
AACTTATTAG

Upstream 100 bases:

>100_bases
CATAAAGAGCCTTGTAAGCGTTATTATTACTAGCATTACAAGCTGTTTTCTGGTATAATCTCAATAGAGATTCAACTAAC
AGAAATTAATTAAACTTCAT

Downstream 100 bases:

>100_bases
TTATTAAATATGAACCAGCCATCCAAAACGTATATGAATAGGAAGTGGTCTATAGTTAGCCATTGTGCTACATTCAATGT
CTAAGTATGTATCTCTTGAT

Product: N6 adenine-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSVAVANKSKPFLHWIGSKRRIVNKLIEHLPQGPHYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYNAVKNNPN
EVNRLLSLYHKHHSKDYYYKVKNKYSNNPNEITAKFIYLNKYSFRGIYRVYKNGQSAQTFSGECYIKLHIASRINQCSSL
LHGVSIYATDFSFIEPQQNDFVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVQELTNKGVKIMISNNNTAFIRDLYKDF
NINTVTVIYSINEQRNPVNELIITNYKTY

Sequences:

>Translated_269_residues
MSVAVANKSKPFLHWIGSKRRIVNKLIEHLPQGPHYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYNAVKNNPN
EVNRLLSLYHKHHSKDYYYKVKNKYSNNPNEITAKFIYLNKYSFRGIYRVYKNGQSAQTFSGECYIKLHIASRINQCSSL
LHGVSIYATDFSFIEPQQNDFVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVQELTNKGVKIMISNNNTAFIRDLYKDF
NINTVTVIYSINEQRNPVNELIITNYKTY
>Mature_268_residues
SVAVANKSKPFLHWIGSKRRIVNKLIEHLPQGPHYNYYEPFLGGGALFFQVRHLFKQCFLSDINLDLITSYNAVKNNPNE
VNRLLSLYHKHHSKDYYYKVKNKYSNNPNEITAKFIYLNKYSFRGIYRVYKNGQSAQTFSGECYIKLHIASRINQCSSLL
HGVSIYATDFSFIEPQQNDFVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVQELTNKGVKIMISNNNTAFIRDLYKDFN
INTVTVIYSINEQRNPVNELIITNYKTY

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=279, Percent_Identity=28.3154121863799, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31740; Mature: 31609

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAVANKSKPFLHWIGSKRRIVNKLIEHLPQGPHYNYYEPFLGGGALFFQVRHLFKQCF
CCEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSDINLDLITSYNAVKNNPNEVNRLLSLYHKHHSKDYYYKVKNKYSNNPNEITAKFIYLN
HCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEE
KYSFRGIYRVYKNGQSAQTFSGECYIKLHIASRINQCSSLLHGVSIYATDFSFIEPQQND
CCCHHHHHHHHHCCCCCCEECCEEEEEEEHHHHHHHHHHHHHCCEEEEECCEEECCCCCC
FVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVQELTNKGVKIMISNNNTAFIRDLYKDF
EEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHC
NINTVTVIYSINEQRNPVNELIITNYKTY
CCCEEEEEEEECCCCCCHHHEEEECCCCC
>Mature Secondary Structure 
SVAVANKSKPFLHWIGSKRRIVNKLIEHLPQGPHYNYYEPFLGGGALFFQVRHLFKQCF
CEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSDINLDLITSYNAVKNNPNEVNRLLSLYHKHHSKDYYYKVKNKYSNNPNEITAKFIYLN
HCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEE
KYSFRGIYRVYKNGQSAQTFSGECYIKLHIASRINQCSSLLHGVSIYATDFSFIEPQQND
CCCHHHHHHHHHCCCCCCEECCEEEEEEEHHHHHHHHHHHHHCCEEEEECCEEECCCCCC
FVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVQELTNKGVKIMISNNNTAFIRDLYKDF
EEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHC
NINTVTVIYSINEQRNPVNELIITNYKTY
CCCEEEEEEEECCCCCCHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]