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The map label for this gene is sucB [H]

Identifier: 189183536

GI number: 189183536

Start: 656500

End: 657777

Strand: Direct

Name: sucB [H]

Synonym: OTT_0629

Alternate gene names: 189183536

Gene position: 656500-657777 (Clockwise)

Preceding gene: 189183535

Following gene: 189183537

Centisome position: 32.68

GC content: 31.14

Gene sequence:

>1278_bases
ATGAAAGAAACAAATCAGGACACAAATATAGTATTACCATCACTTGGAGAGTCAGTGTCAACAGGAACAATATCTAAATG
GCATAAAAAAGAAGGTGATATAGTTGCGCTAGATGAGAAAATAGTAGAGGTGGAAAGTGACAAAGTAGGAATAGATATTA
ACGCTAATGTACCTGGAAAAATAACTAAAATTCTAAAAAACGAAGGAGATAATGTTGAAGTTGGAGAAGTAATTTGCATT
ATTAGATCAGATGTTTCACAGAAAGAAATTCATAGCAGTAAAAGTAGTGATACTGATATAACTCTATCAAGTTGTGAAAA
TATTATTTCTGCTAATAAGTTGTCTCCTGCGGTTGCTAAAATGGTAGCAGAGCATAAAATCAATCCTGAGAATATTAGTG
GTAGTGGAAAAAATAATAGAATTACAAAAGGTGATATTATTAATGTTATAGATTCAAACTTAAATAATAACAATATAACA
TCAATAAATGATATACAAACTCAGCAATTACAGATACCAACATTAATACGTGATACATCAACGCAAACAGCATCAGTGTT
AACTGCTGTAAAAGCTAGTAGAACAATAGAACGAGTAAAAATGACTAGATTGAGACGTACTATTGCTCAACGCTTAAAAG
ATTCTCAAAACAATGCTGCCATTCTATCTACCTTTAATGAAGTTGATATGTTTAATGTATCAGAATTAAGGAAAAAATAT
AAAGAAGAATTTGAAAAAAAGCATGAAATTAAACTTGGTTTTATGTCATTTTTTGTTAAAGCGGCAATTACAGCTTTACA
AGAGCTTCCAATAATTAATGCTCAAGTTGATGGTAATGACATACTATATCACAATTACTGCGATATAGGAGTAGCAGTAA
GTACTAGTTCTGGATTAGTTGTGCCAATAATAAGAAATGCTGAACATCTTTCTTTTGCTGAGATAGAAATGGAAATTTCT
CAGTTAGGTAAAAAAGCTAGAGAAGGAAATTTATCGATTAATGAACTTAGTGGTGGAACTTTCTCTATTACTAATGGAGG
AGTGTTTGGCTCATTGCTGTCAACTCCAATTATTAATCCGCCTCAATCTGCAATAATGGGAATGCATAAAATACAGGACA
GGCCTGTAGTAATTAATGGCACTATACAAATTAGGCCAATGATGTATATAGTATTATCATATGATCACAGAATTATTGAT
GGTAAGGAAGCAGTGACATTTTTAACTAAAGTAAAAAGCTATATAGAATCACCAGAAAGGCTATTGCTTAATATTTAA

Upstream 100 bases:

>100_bases
GTGCTTCAGCTCCTGCAGTAGGATATAATAATCTTCATAATAAGCAGCAGCAAGAATTAATACGGTGTGCTTTTAGTATA
GATATAGGAGAAAGAACATA

Downstream 100 bases:

>100_bases
TTTTTATGAAAAAGAAAATAACTGTTGTTATTATAGTATTAGTAGCTTTAATAACTGTTAATTTTGCTGCTGATGGATGC
GGAGAAAAGTTTATGGTATT

Product: dihydrolipoamide acetyltransferase component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKETNQDTNIVLPSLGESVSTGTISKWHKKEGDIVALDEKIVEVESDKVGIDINANVPGKITKILKNEGDNVEVGEVICI
IRSDVSQKEIHSSKSSDTDITLSSCENIISANKLSPAVAKMVAEHKINPENISGSGKNNRITKGDIINVIDSNLNNNNIT
SINDIQTQQLQIPTLIRDTSTQTASVLTAVKASRTIERVKMTRLRRTIAQRLKDSQNNAAILSTFNEVDMFNVSELRKKY
KEEFEKKHEIKLGFMSFFVKAAITALQELPIINAQVDGNDILYHNYCDIGVAVSTSSGLVVPIIRNAEHLSFAEIEMEIS
QLGKKAREGNLSINELSGGTFSITNGGVFGSLLSTPIINPPQSAIMGMHKIQDRPVVINGTIQIRPMMYIVLSYDHRIID
GKEAVTFLTKVKSYIESPERLLLNI

Sequences:

>Translated_425_residues
MKETNQDTNIVLPSLGESVSTGTISKWHKKEGDIVALDEKIVEVESDKVGIDINANVPGKITKILKNEGDNVEVGEVICI
IRSDVSQKEIHSSKSSDTDITLSSCENIISANKLSPAVAKMVAEHKINPENISGSGKNNRITKGDIINVIDSNLNNNNIT
SINDIQTQQLQIPTLIRDTSTQTASVLTAVKASRTIERVKMTRLRRTIAQRLKDSQNNAAILSTFNEVDMFNVSELRKKY
KEEFEKKHEIKLGFMSFFVKAAITALQELPIINAQVDGNDILYHNYCDIGVAVSTSSGLVVPIIRNAEHLSFAEIEMEIS
QLGKKAREGNLSINELSGGTFSITNGGVFGSLLSTPIINPPQSAIMGMHKIQDRPVVINGTIQIRPMMYIVLSYDHRIID
GKEAVTFLTKVKSYIESPERLLLNI
>Mature_425_residues
MKETNQDTNIVLPSLGESVSTGTISKWHKKEGDIVALDEKIVEVESDKVGIDINANVPGKITKILKNEGDNVEVGEVICI
IRSDVSQKEIHSSKSSDTDITLSSCENIISANKLSPAVAKMVAEHKINPENISGSGKNNRITKGDIINVIDSNLNNNNIT
SINDIQTQQLQIPTLIRDTSTQTASVLTAVKASRTIERVKMTRLRRTIAQRLKDSQNNAAILSTFNEVDMFNVSELRKKY
KEEFEKKHEIKLGFMSFFVKAAITALQELPIINAQVDGNDILYHNYCDIGVAVSTSSGLVVPIIRNAEHLSFAEIEMEIS
QLGKKAREGNLSINELSGGTFSITNGGVFGSLLSTPIINPPQSAIMGMHKIQDRPVVINGTIQIRPMMYIVLSYDHRIID
GKEAVTFLTKVKSYIESPERLLLNI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=239, Percent_Identity=58.5774058577406, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=30.0699300699301, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI31711992, Length=453, Percent_Identity=28.0353200883002, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=28.3185840707965, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=28.3813747228381, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.2670807453416, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1786946, Length=421, Percent_Identity=42.9928741092637, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1786305, Length=418, Percent_Identity=26.0765550239234, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=417, Percent_Identity=40.0479616306954, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=29.3302540415704, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=28.3062645011601, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=28.8888888888889, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=418, Percent_Identity=39.4736842105263, Blast_Score=309, Evalue=6e-85,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=27.3913043478261, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=56.3876651982379, Blast_Score=291, Evalue=8e-79,
Organism=Drosophila melanogaster, GI18859875, Length=439, Percent_Identity=27.7904328018223, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=30.3030303030303, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=30.3030303030303, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 46826; Mature: 46826

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETNQDTNIVLPSLGESVSTGTISKWHKKEGDIVALDEKIVEVESDKVGIDINANVPGK
CCCCCCCCEEECCCCCCCCCCCCCHHHHCCCCCEEEECCEEEEECCCEEEEEECCCCCHH
ITKILKNEGDNVEVGEVICIIRSDVSQKEIHSSKSSDTDITLSSCENIISANKLSPAVAK
HHHHHHCCCCCEEHHHEEEEEECCCCHHHHHCCCCCCCCEEHHHHHHHHHCCCCCHHHHH
MVAEHKINPENISGSGKNNRITKGDIINVIDSNLNNNNITSINDIQTQQLQIPTLIRDTS
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEECCCCCCEEECCHHEECCC
TQTASVLTAVKASRTIERVKMTRLRRTIAQRLKDSQNNAAILSTFNEVDMFNVSELRKKY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
KEEFEKKHEIKLGFMSFFVKAAITALQELPIINAQVDGNDILYHNYCDIGVAVSTSSGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCEEEEEECCCCEE
VPIIRNAEHLSFAEIEMEISQLGKKAREGNLSINELSGGTFSITNGGVFGSLLSTPIINP
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHCCCCCC
PQSAIMGMHKIQDRPVVINGTIQIRPMMYIVLSYDHRIIDGKEAVTFLTKVKSYIESPER
CHHHHHHHHHHCCCCEEEECEEEEEEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHH
LLLNI
HEECC
>Mature Secondary Structure
MKETNQDTNIVLPSLGESVSTGTISKWHKKEGDIVALDEKIVEVESDKVGIDINANVPGK
CCCCCCCCEEECCCCCCCCCCCCCHHHHCCCCCEEEECCEEEEECCCEEEEEECCCCCHH
ITKILKNEGDNVEVGEVICIIRSDVSQKEIHSSKSSDTDITLSSCENIISANKLSPAVAK
HHHHHHCCCCCEEHHHEEEEEECCCCHHHHHCCCCCCCCEEHHHHHHHHHCCCCCHHHHH
MVAEHKINPENISGSGKNNRITKGDIINVIDSNLNNNNITSINDIQTQQLQIPTLIRDTS
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEECCCCCCEEECCHHEECCC
TQTASVLTAVKASRTIERVKMTRLRRTIAQRLKDSQNNAAILSTFNEVDMFNVSELRKKY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
KEEFEKKHEIKLGFMSFFVKAAITALQELPIINAQVDGNDILYHNYCDIGVAVSTSSGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCEEEEEECCCCEE
VPIIRNAEHLSFAEIEMEISQLGKKAREGNLSINELSGGTFSITNGGVFGSLLSTPIINP
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHCCCCCC
PQSAIMGMHKIQDRPVVINGTIQIRPMMYIVLSYDHRIIDGKEAVTFLTKVKSYIESPER
CHHHHHHHHHHCCCCEEEECEEEEEEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHH
LLLNI
HEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA