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The map label for this gene is sppA [H]

Identifier: 189026220

GI number: 189026220

Start: 1084088

End: 1085884

Strand: Reverse

Name: sppA [H]

Synonym: TPASS_0997

Alternate gene names: 189026220

Gene position: 1085884-1084088 (Counterclockwise)

Preceding gene: 189026221

Following gene: 189026219

Centisome position: 95.3

GC content: 51.09

Gene sequence:

>1797_bases
ATGTACTCATTGAGAGTTCTCTTTAGGAGGCTGCTTCGTGGGTTAAATGCCGTTCGGTTGGTCAGTTTGAACGTCATTTT
CTTTCTTTTGTTGTTCTTGTTTTTCAAAGTGCTAGTTGGTGACCGTAGCCGAAGGCCGTCTCAGAAGGTGGTACAATCTG
GGACCGTGTTGCTGATGAGACCGGTTGGCGTTGTTACTGAGCAACGGCCGCGTGTTAATTTGGGCACCGTGTTCTTGAAC
CCAAAGGGATCGGCGATCCTTCTTTCTGATATTACCCAGGCGCTGCGCAACGCAGCCACCGATCGACGCATTGAGTCCGT
TTTTTTCGATCTGTCCAATATGAGTGGTTGGACGAGCGGACATTTTGTGGAAATGGAATCCGCTCTGTCTGAGTATAAAA
AGTCAAAAAAGCCGCTCTACGTTTTTTCTACCTCTTACAGTTTGGCTGACTATTACATCGCCTCTTTTGCTGATGAAATT
ATCCTTGATCCGATGGGGTCTGTGGATCTGTCGGGCTTTTACACGGAAACTCTCTTTTACGSAGGTATGGAGGAAAAGAT
TGGGGTGCGTTGGAACGYCGTGCATGCTGGGGTGTACAAGGGCATGGCTGAGATCTTTTCTAGGAAGGATTTTTCTCCTG
AGGTTCGCAGAAATTATCAGTCTGTATTTGCGCGTCTGTGGCAGCAGTATCTCAGTGATGTTTCGCGTAATCGAGCACTA
GAGGTGCAGCATCTTGCCCGTTACGCGGATCGTCGCCTTGAGCTCCTGCAGAAGTATAACGGAGACGGTGCGCGCACCGC
ATTGGCGGAAAAGTTAGTAACGCGCGTATGTTCCTACGATGAAGCTGGCGTTGCGCTCAAATTTTTAAAAGAAGACGACT
ACGAATCTGCAAAAAATTTCGTTGGTCTAGACGATTATAATCGTGACCGTGCACAGCGGCAGGTGCAGGATCAGGTGGGG
ATTATTCATCTTGCAGGACCGATTGCTGCACACAGGGATACGGAACTCGGCGGAACGATCAGCGACGAGGTTAGTGCTTT
GTTGGATGTCGCGATGAGTGATCCGGATATTAAGGCAGTAGTGTTGCGTATTGATTCCGGTGGGGGAGAGGTGTTTGCTT
CTGAACGTATCCGCCGCGCGCTTGCGCGGGCAAAGCGTCGAGGCAAGAAGCCAGTGATAGTATCGATGGGTGCGATTGCT
GCGTCTGGTGCGTACTGGGTTGCTTCTGCAGCCGATTACATCTTCGCATCCCCCTATACCATCACTGGTTCCATAGGGGT
GCTTTCGGTACTACCGACATTCGAAACGTTTTTAGAGCGATATGCGGGGATCACTGTCGATAGCGTACAGGTGCACGGCG
TTCGCCAACCTTCTTTGCTCAGGAGTGGAACGGCTGAAGACACCGCGCGCATGCAGCTTGATGTGATGGCGACGTATCGT
ACTTTTCTTTCGGTTGTTTCTGCCGGGCGTAACCTTACCCTTGATCGGGTGGCGGCGGTTGCAGAGGGTAGGATTTACGC
GGGGGAGGACGCAGTTTCCCTAGGCTTGGTTGATGCGCTAGGCGGACTAGATGAAGCGGTAGCACATGCAGCGAAAGAAT
CACATTGCAGGCAGTATTCGGTGAGAGTTTTGAAGCGGAGCGCCACGTACGGTGAAGAATTTCTGCAGTCCCTGTGGGAT
GTCCTGCAGAAACGAAGTCTTGCTTTTGGAGAGCGTGTGATCATTGGAGAGTTACTCCAGCTTGACCTAAGCAAGGGCAC
CTACGTATATGAGCCGCTGCGCTTGCATTGGCGTTGA

Upstream 100 bases:

>100_bases
TGGGAATGTGCCAGTCTTCTTTCAGGAACATTTGGTATTTCCTGAAAAGATGGCCTGCCGGGAGTAGACGAGGCATAACA
CACATGAGGTGGAGGTTACA

Downstream 100 bases:

>100_bases
CGGGCACTGCTACGCTTGATCGAGCGCAGCGTTGTTTGCTACGGTTGGCGCCGGTTTTTGGGGATGTAGCTCAGTTGGTT
AGAGCGCTTGCATGGCATGC

Product: protease IV

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MYSLRVLFRRLLRGLNAVRLVSLNVIFFLLLFLFFKVLVGDRSRRPSQKVVQSGTVLLMRPVGVVTEQRPRVNLGTVFLN
PKGSAILLSDITQALRNAATDRRIESVFFDLSNMSGWTSGHFVEMESALSEYKKSKKPLYVFSTSYSLADYYIASFADEI
ILDPMGSVDLSGFYTETLFYXGMEEKIGVRWNXVHAGVYKGMAEIFSRKDFSPEVRRNYQSVFARLWQQYLSDVSRNRAL
EVQHLARYADRRLELLQKYNGDGARTALAEKLVTRVCSYDEAGVALKFLKEDDYESAKNFVGLDDYNRDRAQRQVQDQVG
IIHLAGPIAAHRDTELGGTISDEVSALLDVAMSDPDIKAVVLRIDSGGGEVFASERIRRALARAKRRGKKPVIVSMGAIA
ASGAYWVASAADYIFASPYTITGSIGVLSVLPTFETFLERYAGITVDSVQVHGVRQPSLLRSGTAEDTARMQLDVMATYR
TFLSVVSAGRNLTLDRVAAVAEGRIYAGEDAVSLGLVDALGGLDEAVAHAAKESHCRQYSVRVLKRSATYGEEFLQSLWD
VLQKRSLAFGERVIIGELLQLDLSKGTYVYEPLRLHWR

Sequences:

>Translated_598_residues
MYSLRVLFRRLLRGLNAVRLVSLNVIFFLLLFLFFKVLVGDRSRRPSQKVVQSGTVLLMRPVGVVTEQRPRVNLGTVFLN
PKGSAILLSDITQALRNAATDRRIESVFFDLSNMSGWTSGHFVEMESALSEYKKSKKPLYVFSTSYSLADYYIASFADEI
ILDPMGSVDLSGFYTETLFYXGMEEKIGVRWNXVHAGVYKGMAEIFSRKDFSPEVRRNYQSVFARLWQQYLSDVSRNRAL
EVQHLARYADRRLELLQKYNGDGARTALAEKLVTRVCSYDEAGVALKFLKEDDYESAKNFVGLDDYNRDRAQRQVQDQVG
IIHLAGPIAAHRDTELGGTISDEVSALLDVAMSDPDIKAVVLRIDSGGGEVFASERIRRALARAKRRGKKPVIVSMGAIA
ASGAYWVASAADYIFASPYTITGSIGVLSVLPTFETFLERYAGITVDSVQVHGVRQPSLLRSGTAEDTARMQLDVMATYR
TFLSVVSAGRNLTLDRVAAVAEGRIYAGEDAVSLGLVDALGGLDEAVAHAAKESHCRQYSVRVLKRSATYGEEFLQSLWD
VLQKRSLAFGERVIIGELLQLDLSKGTYVYEPLRLHWR
>Mature_598_residues
MYSLRVLFRRLLRGLNAVRLVSLNVIFFLLLFLFFKVLVGDRSRRPSQKVVQSGTVLLMRPVGVVTEQRPRVNLGTVFLN
PKGSAILLSDITQALRNAATDRRIESVFFDLSNMSGWTSGHFVEMESALSEYKKSKKPLYVFSTSYSLADYYIASFADEI
ILDPMGSVDLSGFYTETLFYXGMEEKIGVRWNXVHAGVYKGMAEIFSRKDFSPEVRRNYQSVFARLWQQYLSDVSRNRAL
EVQHLARYADRRLELLQKYNGDGARTALAEKLVTRVCSYDEAGVALKFLKEDDYESAKNFVGLDDYNRDRAQRQVQDQVG
IIHLAGPIAAHRDTELGGTISDEVSALLDVAMSDPDIKAVVLRIDSGGGEVFASERIRRALARAKRRGKKPVIVSMGAIA
ASGAYWVASAADYIFASPYTITGSIGVLSVLPTFETFLERYAGITVDSVQVHGVRQPSLLRSGTAEDTARMQLDVMATYR
TFLSVVSAGRNLTLDRVAAVAEGRIYAGEDAVSLGLVDALGGLDEAVAHAAKESHCRQYSVRVLKRSATYGEEFLQSLWD
VLQKRSLAFGERVIIGELLQLDLSKGTYVYEPLRLHWR

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=565, Percent_Identity=33.9823008849557, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1787527, Length=218, Percent_Identity=30.7339449541284, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 66627; Mature: 66627

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSLRVLFRRLLRGLNAVRLVSLNVIFFLLLFLFFKVLVGDRSRRPSQKVVQSGTVLLMR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEE
PVGVVTEQRPRVNLGTVFLNPKGSAILLSDITQALRNAATDRRIESVFFDLSNMSGWTSG
CCCHHCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HFVEMESALSEYKKSKKPLYVFSTSYSLADYYIASFADEIILDPMGSVDLSGFYTETLFY
CHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
XGMEEKIGVRWNXVHAGVYKGMAEIFSRKDFSPEVRRNYQSVFARLWQQYLSDVSRNRAL
CCCCHHCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EVQHLARYADRRLELLQKYNGDGARTALAEKLVTRVCSYDEAGVALKFLKEDDYESAKNF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHCCCHHHHHCC
VGLDDYNRDRAQRQVQDQVGIIHLAGPIAAHRDTELGGTISDEVSALLDVAMSDPDIKAV
CCCCCCCHHHHHHHHHHHCCEEEEECCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
VLRIDSGGGEVFASERIRRALARAKRRGKKPVIVSMGAIAASGAYWVASAADYIFASPYT
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHCCCCHHHHHHHHHHEECCEE
ITGSIGVLSVLPTFETFLERYAGITVDSVQVHGVRQPSLLRSGTAEDTARMQLDVMATYR
EECCCHHHHHHHHHHHHHHHHCCCEEECEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHH
TFLSVVSAGRNLTLDRVAAVAEGRIYAGEDAVSLGLVDALGGLDEAVAHAAKESHCRQYS
HHHHHHHCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VRVLKRSATYGEEFLQSLWDVLQKRSLAFGERVIIGELLQLDLSKGTYVYEPLRLHWR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCEECCC
>Mature Secondary Structure
MYSLRVLFRRLLRGLNAVRLVSLNVIFFLLLFLFFKVLVGDRSRRPSQKVVQSGTVLLMR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEE
PVGVVTEQRPRVNLGTVFLNPKGSAILLSDITQALRNAATDRRIESVFFDLSNMSGWTSG
CCCHHCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
HFVEMESALSEYKKSKKPLYVFSTSYSLADYYIASFADEIILDPMGSVDLSGFYTETLFY
CHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
XGMEEKIGVRWNXVHAGVYKGMAEIFSRKDFSPEVRRNYQSVFARLWQQYLSDVSRNRAL
CCCCHHCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EVQHLARYADRRLELLQKYNGDGARTALAEKLVTRVCSYDEAGVALKFLKEDDYESAKNF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHCCCHHHHHCC
VGLDDYNRDRAQRQVQDQVGIIHLAGPIAAHRDTELGGTISDEVSALLDVAMSDPDIKAV
CCCCCCCHHHHHHHHHHHCCEEEEECCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
VLRIDSGGGEVFASERIRRALARAKRRGKKPVIVSMGAIAASGAYWVASAADYIFASPYT
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHCCCCHHHHHHHHHHEECCEE
ITGSIGVLSVLPTFETFLERYAGITVDSVQVHGVRQPSLLRSGTAEDTARMQLDVMATYR
EECCCHHHHHHHHHHHHHHHHCCCEEECEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHH
TFLSVVSAGRNLTLDRVAAVAEGRIYAGEDAVSLGLVDALGGLDEAVAHAAKESHCRQYS
HHHHHHHCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VRVLKRSATYGEEFLQSLWDVLQKRSLAFGERVIIGELLQLDLSKGTYVYEPLRLHWR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3522590; 9097039; 9278503; 7973728 [H]