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The map label for this gene is nadE

Identifier: 189026005

GI number: 189026005

Start: 847773

End: 849812

Strand: Reverse

Name: nadE

Synonym: TPASS_0780

Alternate gene names: 189026005

Gene position: 849812-847773 (Counterclockwise)

Preceding gene: 189026006

Following gene: 189026004

Centisome position: 74.58

GC content: 55.42

Gene sequence:

>2040_bases
GTGAGGGGGTGTGGTGTGGATAGGACGGGTGGATACGTGCGGCTTGCGCTTGCAGCCCCTGCGGTGCGTGTTGCGGACTG
TGCATACAATACCCAGCGTATGATTCAGACGGTGCGTCGTGCAGCTTCATGCGGTGTGGACATACTATTGTTTCCCCGTC
TTTCGCTTACAGGGTGTAGCTGTGCGTCTCTTTTTGCTCAGGATACGCTGCTTTCGGCAGTCTGCACGCACGTATCTGCA
CTGTGTGCTGGCACTGCTGATTGTCAGCTGTTAGCGCTTGTGAGTGTGCCCTGTTTTTTGCGCACTCAGGTGCGCGTGTG
TACTGCGCTTGTCGCACGAGGTCGTGTTCTAGCACTGGTTGTGCAGGATACCCTGGCGGCGTGTGGCGCGCAAAAAATGC
AAGTGCCCTGTGAGGTCCTGTACGGTGGTGCACCGGTGCCGGTGTACGATGTGCAGACGTGTTTTGAAAGTGCAGAGGGT
CTTTTCTCTTTTTGTGTTGGTGCTATGGATGGATCGGTACCTGCCACGCTGGTGTTGCAGGCCTACGGTACGCCAAGTAC
GGCGCAGACACCGGATATTTTTGCTGCGCACGCTGCGGCATACAGTGCACAGCACCAATGTGCGTATGCGTACGTAAATG
CGGGGTGGGGGGAGTCTAGTGCTGATGCGGTGTATGGCGCGGAAAGTGGTATTTTTGAGTGTGGGCAGTGTGTGGTCCAA
GACTCATTGCAGGAGATGCGAGAACGGGGGGAGCGTCCGGCGCACGCGGTGCGTGGACTGCATGTTAGTGCGGACGTAGA
TGTGTCTTTGGTACACTTTCGTCGTCGTGCGCGTAGCGGACATACCACTCTGGGTGCATCGGCTCCCTGCGTCACGCTTC
CTGCAGGCATATTTGCAGCGTCAAAGGCGCACGCCACGCTGCGGCGTCCTCGCGTACCCTGTCCTTTTTTTCCGCCTGCT
TTTCAAAAATCGCAGGATGCGGTGCCCCCGCTCACGGGTGCCGTGTGCCTCGCTGTTTCTGCACCGTCAGACACGCAGGA
CGGTTTTTTGCAAAGAACGATAGACTTAGCCGCGCAGGGCGTGGCACTCCGTCTTGAACACATGGGCTGTAGGCGCCTGG
TGGTGGGTGTTTCAGGAGGTGTTGATTCGGCGTGTGCATTGCTAATATGCGCGCGCGCGTTAGATTTTCTCTCGATTGCG
CGTACACAACTTTATGCGCTAACGCTTCCTGGCTTTGGTACTACGTCAGGAACGAAAGGTGCGGCGCAGGAGTTTGCGCG
TGCGCTCGGTTGCACTGTGCAAGAAATTTCTATTAGCGCGGCAGTGACGCATCATCTCCATGATATTGGGCATACGATGC
AGCAGTGTGACGGTACGTATGAGAATGCACAGGCGCGCGAACGGACGCAGATTTTGTTAGATCGTGCTAACCAGCTTGAT
GCGCTCATGATTGGTACGGGAGATGCGTCAGAAGGTGCGCTTGGTTGGGAAACCTTTGGGGGCGATCACCTTTCGCTGTA
CGCAGTGAACGCATCTTTGCCCAAAACCGTGGTGCGAGCCTTGATTTCCTATGCTGGGCGTGTACCTGAGCGTTTTGTGT
GTGAAACTGATTCTCCCTATGCACCGCGCGGTGCTGCCTTTTCTCGCGTTTGTGCAGCTATAGTTGCACAGCCGGTGAGT
CCTGAGCTCATACCTCCTTGTGATGATCGTATTGTGCAGTGTACCGAGGAGATGCTCGGTCCTTATGAATTGCATGATTT
TTTTCTGTATCACATAACGGTGAACGGTTTTGGTCCTCGAAAACTTTTTCGTGTGGCCGCGCATGCGTTTGGAAMTGCGT
ATTCTTGCGCGCAGCTATGTGCAGCGCTGCGCGTTTTTTTTACCCGCTTGTTTTCACAGCAGTTCAAGCGTTCTTGTGTG
CCTGATGGGCCCGGTCTTACGGAAGTGAACCTTTCCCCTCGTGTGGGTTTTTATTTTCCCAGCGACACTTCCGGTGCGCT
ATGGCGCGCAGAGCTTGAGCAGCTGGCTTGTGGGGAATAG

Upstream 100 bases:

>100_bases
GATTAGCGCGCGTGATATTTCAGGCAATGAACGAACGGAAGTGTTCTTTTTACAGGTGGAGTAAGGCGTTCGTAGTTTTT
TCATTTGTACACCGGGGTGT

Downstream 100 bases:

>100_bases
ACTGGCACGCAGGATTTTTAACAACTGATATGGAGGTGCGTAGGGCGTGGTGCATACGCTTTTTTCTTGGGTTTCAGCGC
ATATTCACTCGTTACCTATG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing]

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MRGCGVDRTGGYVRLALAAPAVRVADCAYNTQRMIQTVRRAASCGVDILLFPRLSLTGCSCASLFAQDTLLSAVCTHVSA
LCAGTADCQLLALVSVPCFLRTQVRVCTALVARGRVLALVVQDTLAACGAQKMQVPCEVLYGGAPVPVYDVQTCFESAEG
LFSFCVGAMDGSVPATLVLQAYGTPSTAQTPDIFAAHAAAYSAQHQCAYAYVNAGWGESSADAVYGAESGIFECGQCVVQ
DSLQEMRERGERPAHAVRGLHVSADVDVSLVHFRRRARSGHTTLGASAPCVTLPAGIFAASKAHATLRRPRVPCPFFPPA
FQKSQDAVPPLTGAVCLAVSAPSDTQDGFLQRTIDLAAQGVALRLEHMGCRRLVVGVSGGVDSACALLICARALDFLSIA
RTQLYALTLPGFGTTSGTKGAAQEFARALGCTVQEISISAAVTHHLHDIGHTMQQCDGTYENAQARERTQILLDRANQLD
ALMIGTGDASEGALGWETFGGDHLSLYAVNASLPKTVVRALISYAGRVPERFVCETDSPYAPRGAAFSRVCAAIVAQPVS
PELIPPCDDRIVQCTEEMLGPYELHDFFLYHITVNGFGPRKLFRVAAHAFGXAYSCAQLCAALRVFFTRLFSQQFKRSCV
PDGPGLTEVNLSPRVGFYFPSDTSGALWRAELEQLACGE

Sequences:

>Translated_679_residues
MRGCGVDRTGGYVRLALAAPAVRVADCAYNTQRMIQTVRRAASCGVDILLFPRLSLTGCSCASLFAQDTLLSAVCTHVSA
LCAGTADCQLLALVSVPCFLRTQVRVCTALVARGRVLALVVQDTLAACGAQKMQVPCEVLYGGAPVPVYDVQTCFESAEG
LFSFCVGAMDGSVPATLVLQAYGTPSTAQTPDIFAAHAAAYSAQHQCAYAYVNAGWGESSADAVYGAESGIFECGQCVVQ
DSLQEMRERGERPAHAVRGLHVSADVDVSLVHFRRRARSGHTTLGASAPCVTLPAGIFAASKAHATLRRPRVPCPFFPPA
FQKSQDAVPPLTGAVCLAVSAPSDTQDGFLQRTIDLAAQGVALRLEHMGCRRLVVGVSGGVDSACALLICARALDFLSIA
RTQLYALTLPGFGTTSGTKGAAQEFARALGCTVQEISISAAVTHHLHDIGHTMQQCDGTYENAQARERTQILLDRANQLD
ALMIGTGDASEGALGWETFGGDHLSLYAVNASLPKTVVRALISYAGRVPERFVCETDSPYAPRGAAFSRVCAAIVAQPVS
PELIPPCDDRIVQCTEEMLGPYELHDFFLYHITVNGFGPRKLFRVAAHAFGXAYSCAQLCAALRVFFTRLFSQQFKRSCV
PDGPGLTEVNLSPRVGFYFPSDTSGALWRAELEQLACGE
>Mature_679_residues
MRGCGVDRTGGYVRLALAAPAVRVADCAYNTQRMIQTVRRAASCGVDILLFPRLSLTGCSCASLFAQDTLLSAVCTHVSA
LCAGTADCQLLALVSVPCFLRTQVRVCTALVARGRVLALVVQDTLAACGAQKMQVPCEVLYGGAPVPVYDVQTCFESAEG
LFSFCVGAMDGSVPATLVLQAYGTPSTAQTPDIFAAHAAAYSAQHQCAYAYVNAGWGESSADAVYGAESGIFECGQCVVQ
DSLQEMRERGERPAHAVRGLHVSADVDVSLVHFRRRARSGHTTLGASAPCVTLPAGIFAASKAHATLRRPRVPCPFFPPA
FQKSQDAVPPLTGAVCLAVSAPSDTQDGFLQRTIDLAAQGVALRLEHMGCRRLVVGVSGGVDSACALLICARALDFLSIA
RTQLYALTLPGFGTTSGTKGAAQEFARALGCTVQEISISAAVTHHLHDIGHTMQQCDGTYENAQARERTQILLDRANQLD
ALMIGTGDASEGALGWETFGGDHLSLYAVNASLPKTVVRALISYAGRVPERFVCETDSPYAPRGAAFSRVCAAIVAQPVS
PELIPPCDDRIVQCTEEMLGPYELHDFFLYHITVNGFGPRKLFRVAAHAFGXAYSCAQLCAALRVFFTRLFSQQFKRSCV
PDGPGLTEVNLSPRVGFYFPSDTSGALWRAELEQLACGE

Specific function: Can use both glutamine or ammonia as a nitrogen source

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the NAD synthetase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADE_TREPA (O83759)

Other databases:

- EMBL:   AE000520
- PIR:   E71282
- RefSeq:   NP_219217.1
- ProteinModelPortal:   O83759
- SMR:   O83759
- GeneID:   2611325
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0780
- NMPDR:   fig|243276.1.peg.776
- TIGR:   TP_0780
- HOGENOM:   HBG732101
- OMA:   VERYMEN
- ProtClustDB:   PRK02628
- BioCyc:   TPAL243276:TP_0780-MONOMER
- BRENDA:   6.3.5.1
- HAMAP:   MF_00193
- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.60.110.10
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006630
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase; SSF56317 Ntlse/CNhydtse

EC number: =6.3.5.1

Molecular weight: Translated: 72100; Mature: 72100

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: ACT_SITE 378-378

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGCGVDRTGGYVRLALAAPAVRVADCAYNTQRMIQTVRRAASCGVDILLFPRLSLTGCS
CCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
CASLFAQDTLLSAVCTHVSALCAGTADCQLLALVSVPCFLRTQVRVCTALVARGRVLALV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEH
VQDTLAACGAQKMQVPCEVLYGGAPVPVYDVQTCFESAEGLFSFCVGAMDGSVPATLVLQ
HHHHHHHCCCCCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
AYGTPSTAQTPDIFAAHAAAYSAQHQCAYAYVNAGWGESSADAVYGAESGIFECGQCVVQ
ECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHH
DSLQEMRERGERPAHAVRGLHVSADVDVSLVHFRRRARSGHTTLGASAPCVTLPAGIFAA
HHHHHHHHHCCCHHHHHHCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHH
SKAHATLRRPRVPCPFFPPAFQKSQDAVPPLTGAVCLAVSAPSDTQDGFLQRTIDLAAQG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCC
VALRLEHMGCRRLVVGVSGGVDSACALLICARALDFLSIARTQLYALTLPGFGTTSGTKG
HHEEHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCH
AAQEFARALGCTVQEISISAAVTHHLHDIGHTMQQCDGTYENAQARERTQILLDRANQLD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
ALMIGTGDASEGALGWETFGGDHLSLYAVNASLPKTVVRALISYAGRVPERFVCETDSPY
EEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCC
APRGAAFSRVCAAIVAQPVSPELIPPCDDRIVQCTEEMLGPYELHDFFLYHITVNGFGPR
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCEEEEEEEEECCCCHH
KLFRVAAHAFGXAYSCAQLCAALRVFFTRLFSQQFKRSCVPDGPGLTEVNLSPRVGFYFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECC
SDTSGALWRAELEQLACGE
CCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MRGCGVDRTGGYVRLALAAPAVRVADCAYNTQRMIQTVRRAASCGVDILLFPRLSLTGCS
CCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
CASLFAQDTLLSAVCTHVSALCAGTADCQLLALVSVPCFLRTQVRVCTALVARGRVLALV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEH
VQDTLAACGAQKMQVPCEVLYGGAPVPVYDVQTCFESAEGLFSFCVGAMDGSVPATLVLQ
HHHHHHHCCCCCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
AYGTPSTAQTPDIFAAHAAAYSAQHQCAYAYVNAGWGESSADAVYGAESGIFECGQCVVQ
ECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHH
DSLQEMRERGERPAHAVRGLHVSADVDVSLVHFRRRARSGHTTLGASAPCVTLPAGIFAA
HHHHHHHHHCCCHHHHHHCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHH
SKAHATLRRPRVPCPFFPPAFQKSQDAVPPLTGAVCLAVSAPSDTQDGFLQRTIDLAAQG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCC
VALRLEHMGCRRLVVGVSGGVDSACALLICARALDFLSIARTQLYALTLPGFGTTSGTKG
HHEEHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCH
AAQEFARALGCTVQEISISAAVTHHLHDIGHTMQQCDGTYENAQARERTQILLDRANQLD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
ALMIGTGDASEGALGWETFGGDHLSLYAVNASLPKTVVRALISYAGRVPERFVCETDSPY
EEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCC
APRGAAFSRVCAAIVAQPVSPELIPPCDDRIVQCTEEMLGPYELHDFFLYHITVNGFGPR
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCEEEEEEEEECCCCHH
KLFRVAAHAFGXAYSCAQLCAALRVFFTRLFSQQFKRSCVPDGPGLTEVNLSPRVGFYFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECC
SDTSGALWRAELEQLACGE
CCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876