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The map label for this gene is miaB

Identifier: 189025979

GI number: 189025979

Start: 819624

End: 820994

Strand: Reverse

Name: miaB

Synonym: TPASS_0754

Alternate gene names: 189025979

Gene position: 820994-819624 (Counterclockwise)

Preceding gene: 189025980

Following gene: 189025978

Centisome position: 72.05

GC content: 48.8

Gene sequence:

>1371_bases
TTGACCTATTTTTTTGAAACGTACGGGTGCCAGATGAATGTTGCAGAGTCTGCTTCTGTAGAGCAGCTCCTGTTGGCGCG
GGGGTGGACAAAGGCGGTAGACGCGCAGACGTGCGACGTGCTGATTATCAATACGTGTTCTGTGCGAATTACAGCAGAAA
CGCGGGTCTTTGGGAGACTTGGCTTATTTTCTTCTCTTAAAAAAAAGCGTGCGTTTTTCATTATCCTTATGGGGTGTATG
GCACAGCGTTTACACGACAAAATTCAGCAGCAGTTTCCTCGTATTGATTATGTAGTGGGTACGTTTGCGCACGCGCGATT
TGAATCCATTTTCCAAGAAATTGAACAGAAGCTTACCCAGAAAGATTACCGCTTTGAGTTTATCTCCGAGCGTTACCGGG
AGCATCCTGTCTCTGGGTATCGTTTTTTCGCTTCTTCATATAGCGAAGGTTCATTCCAAAGTTTTATCCCCATCATGAAT
GGCTGCAATAATTTTTGTTCGTTTTGCATTGTGCCATACGTGCGTGGACGGGAGATCTCGCGTGATCTTGATGCTATTTT
GCAGGAAGTGGATGTGCTCTCTGAGAAAGGAGTGCGGGAAATTACGTTGCTCGGACAAAATGTTAATTCGTATCGGGGAA
GAGACCGTGAAGGGAACATAGTTACCTTTCCCCAGCTGTTGCGTCATTTGGTTCGTCGTTGCGAAGTCAAAGATCAGATA
AAGTGGATCCGCTTTGTTTCCAGTCACCCTAAAGACCTTTCTGATGATCTGATTGCTACTATTGCTCAGGAATCTCGTCT
GTGTCGTCTGGTGCATTTGCCAGTGCAGCATGGGGCGAATGGAGTGCTCAAGCGGATGCGACGGAGTTACACGAGAGAGC
AGTATCTGTCGCTGGTGGGTAAACTGAAAGCGAGTGTCCCCAATGTGGCGCTGAGCACAGATATTCTTATTGGGTTCCCG
GGGGAGACGGAGGAGGATTTTGAGCAAACGCTGGATCTCATGCGGGAGGTGGAGTTTGATTCCGCTTTTATGTATCACTA
TAACCCGCGCGAGGGAACGCCTGCCTATGACTTTCCCGATCGTATCCCTGATGCAACGCGGATTGCGCGTCTACAACGCG
TCATTGCTCTGCAGATGAGTACTACTTTGAAAAAGATGCGCGCACGGGTAGGAAAGACATTGCCAGTGTTGGTAGAGTCG
CGCTCGCGAAATAATCCTGAAGAATTGTTTGGACATACAGAGCTTGGGGAAATGACCGTGCTTGAAGGAAAGGTGGATCC
TACGTACATCGGACGCTTTGTGGACGTGCAAGTGAAGGAAGTGCGCGGCAGGACCTTGCGTGCCCATCTGGTGCAGGAGC
GTGCAAAATGA

Upstream 100 bases:

>100_bases
TGCAGACTAACACCGCGCTAGAAGCGTACACCGTGTTGACAGAGCAGTCTAGTTTGTAAGATTTAGAAGAGAGCAGGATT
GTTCAGGCAGAGGGAAAGCA

Downstream 100 bases:

>100_bases
CATATGGAAAGCTGATTTTTTTTATTATCGTACTTGTGGGTTTCGCGCTCTTCATGTCCTTCAACGTGGAACACCGCTGC
GATGTATCGCTTGTCTTTTA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTYFFETYGCQMNVAESASVEQLLLARGWTKAVDAQTCDVLIINTCSVRITAETRVFGRLGLFSSLKKKRAFFIILMGCM
AQRLHDKIQQQFPRIDYVVGTFAHARFESIFQEIEQKLTQKDYRFEFISERYREHPVSGYRFFASSYSEGSFQSFIPIMN
GCNNFCSFCIVPYVRGREISRDLDAILQEVDVLSEKGVREITLLGQNVNSYRGRDREGNIVTFPQLLRHLVRRCEVKDQI
KWIRFVSSHPKDLSDDLIATIAQESRLCRLVHLPVQHGANGVLKRMRRSYTREQYLSLVGKLKASVPNVALSTDILIGFP
GETEEDFEQTLDLMREVEFDSAFMYHYNPREGTPAYDFPDRIPDATRIARLQRVIALQMSTTLKKMRARVGKTLPVLVES
RSRNNPEELFGHTELGEMTVLEGKVDPTYIGRFVDVQVKEVRGRTLRAHLVQERAK

Sequences:

>Translated_456_residues
MTYFFETYGCQMNVAESASVEQLLLARGWTKAVDAQTCDVLIINTCSVRITAETRVFGRLGLFSSLKKKRAFFIILMGCM
AQRLHDKIQQQFPRIDYVVGTFAHARFESIFQEIEQKLTQKDYRFEFISERYREHPVSGYRFFASSYSEGSFQSFIPIMN
GCNNFCSFCIVPYVRGREISRDLDAILQEVDVLSEKGVREITLLGQNVNSYRGRDREGNIVTFPQLLRHLVRRCEVKDQI
KWIRFVSSHPKDLSDDLIATIAQESRLCRLVHLPVQHGANGVLKRMRRSYTREQYLSLVGKLKASVPNVALSTDILIGFP
GETEEDFEQTLDLMREVEFDSAFMYHYNPREGTPAYDFPDRIPDATRIARLQRVIALQMSTTLKKMRARVGKTLPVLVES
RSRNNPEELFGHTELGEMTVLEGKVDPTYIGRFVDVQVKEVRGRTLRAHLVQERAK
>Mature_455_residues
TYFFETYGCQMNVAESASVEQLLLARGWTKAVDAQTCDVLIINTCSVRITAETRVFGRLGLFSSLKKKRAFFIILMGCMA
QRLHDKIQQQFPRIDYVVGTFAHARFESIFQEIEQKLTQKDYRFEFISERYREHPVSGYRFFASSYSEGSFQSFIPIMNG
CNNFCSFCIVPYVRGREISRDLDAILQEVDVLSEKGVREITLLGQNVNSYRGRDREGNIVTFPQLLRHLVRRCEVKDQIK
WIRFVSSHPKDLSDDLIATIAQESRLCRLVHLPVQHGANGVLKRMRRSYTREQYLSLVGKLKASVPNVALSTDILIGFPG
ETEEDFEQTLDLMREVEFDSAFMYHYNPREGTPAYDFPDRIPDATRIARLQRVIALQMSTTLKKMRARVGKTLPVLVESR
SRNNPEELFGHTELGEMTVLEGKVDPTYIGRFVDVQVKEVRGRTLRAHLVQERAK

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=34.2323651452282, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=34.2323651452282, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=26.0393873085339, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1786882, Length=453, Percent_Identity=36.2030905077263, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1787057, Length=434, Percent_Identity=26.2672811059908, Blast_Score=168, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=479, Percent_Identity=29.6450939457203, Blast_Score=228, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=203, Percent_Identity=33.9901477832512, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356207, Length=490, Percent_Identity=32.6530612244898, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI19922432, Length=424, Percent_Identity=27.8301886792453, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_TREPA (O83735)

Other databases:

- EMBL:   AE000520
- PIR:   E71284
- RefSeq:   NP_219191.1
- ProteinModelPortal:   O83735
- SMR:   O83735
- IntAct:   O83735
- GeneID:   2611143
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0754
- NMPDR:   fig|243276.1.peg.750
- TIGR:   TP_0754
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14335
- BioCyc:   TPAL243276:TP_0754-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52573; Mature: 52442

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYFFETYGCQMNVAESASVEQLLLARGWTKAVDAQTCDVLIINTCSVRITAETRVFGRL
CCEEEEECCCEECCCCCCHHHHHHHHCCCCHHCCCCCCCEEEEEEEEEEEEECHHHHHHH
GLFSSLKKKRAFFIILMGCMAQRLHDKIQQQFPRIDYVVGTFAHARFESIFQEIEQKLTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KDYRFEFISERYREHPVSGYRFFASSYSEGSFQSFIPIMNGCNNFCSFCIVPYVRGREIS
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCHHH
RDLDAILQEVDVLSEKGVREITLLGQNVNSYRGRDREGNIVTFPQLLRHLVRRCEVKDQI
HHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCCCCEEEHHHHHHHHHHHCCHHHHH
KWIRFVSSHPKDLSDDLIATIAQESRLCRLVHLPVQHGANGVLKRMRRSYTREQYLSLVG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCHHHCCHHHHHHHHHHHHHHHHHHHHH
KLKASVPNVALSTDILIGFPGETEEDFEQTLDLMREVEFDSAFMYHYNPREGTPAYDFPD
HHHHCCCCCEEECEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
RIPDATRIARLQRVIALQMSTTLKKMRARVGKTLPVLVESRSRNNPEELFGHTELGEMTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCCEEE
LEGKVDPTYIGRFVDVQVKEVRGRTLRAHLVQERAK
EECCCCHHHHHHHHEEHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TYFFETYGCQMNVAESASVEQLLLARGWTKAVDAQTCDVLIINTCSVRITAETRVFGRL
CEEEEECCCEECCCCCCHHHHHHHHCCCCHHCCCCCCCEEEEEEEEEEEEECHHHHHHH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EECCCCHHHHHHHHEEHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876