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The map label for this gene is pfs

Identifier: 189025403

GI number: 189025403

Start: 191268

End: 192077

Strand: Direct

Name: pfs

Synonym: TPASS_0170

Alternate gene names: 189025403

Gene position: 191268-192077 (Clockwise)

Preceding gene: 189025402

Following gene: 189025404

Centisome position: 16.79

GC content: 54.81

Gene sequence:

>810_bases
GTGACGGTCGGCGTTTTTGCTGCACTGGGAGAGGAGGTTGCGCGCGTACGTGAGTGTCTCGGGGGAGTAGGTACAGAGCG
GGCAGGGCTCACGTTCTATGTTGTGTCGGTGGGTGCGCTTCAGGTGGTGTATGTGTGCGGCGGTGTTGGGAAAGTGAATG
CAGCGCTTTGCACTCAACTACTCATTTCGGAGTTTGGTGCGCGTGTGCTTATCAATACAGGAATTGCAGGTGCTCTCGAT
GAGCGCTTGTGTGTTTTTGATGTGCTAGTTTCTGTCGATGCAGTGCAGCATGATGTAGACGTTACCGCTTTTGGTTACCA
GAAAGGGCGTATTCCGCGCATGGATTCTGTAGAGTGGACTGCGAACACGGCATTGCGGTATTTGGTGCGGGAAGCCTTTG
ATCTCTGCACACGGGATCCGGAGTGGACCGAGGGTGCTTGTGCGCTCTCTGGCTCGGGGGATCCGCCTTCTCGCGTTTCT
CGTTTAGTCGAAGGGCGCGTCGCCTCAGGGGATCTTTTCGTGTCAGATGCGCAAACACGTGCACGCATTATACGCGAATT
TGGTGCGCATGGTGTGGAAATGGAGGGGGCAGCCTTTGCGCATGTAGCGTCAGTTAATGGCGTCCCCTTTGTTATTATAC
GCTGTATATCCGACGGTGCGGGTGCTGAGCAGGATGTCTCTATGAGTTATAAAGAGTTTTCGACGCGTGCGGCGCGGCGT
TCTGCCCTTTTGACGTTGCGTGTTCTTGAGCGTCTCAGCGCTTTACGCACATCGGTAGTGGCTTCTCTTTTCCCGATGGT
CGTGGTGTAA

Upstream 100 bases:

>100_bases
GTGCCTTGTGGTCGGGGCTTTTTCCTGACCTTCAAGGGACAGCTTTTTTCCGTGCCTGGGTTGCGAGTGCGCGTTTTCGT
GTTTTTCACGGGGAGGGTGC

Downstream 100 bases:

>100_bases
TGGTCGGGAGTAAGACAGTGGGCTGAGCAGTCGGGCGATGCTGTGGGGGGCTTGTGTAGGTGTATACCCAGTTGTTATAC
TGCGGCACTCGGAGGTTTTA

Product: protein Pfs

Products: NA

Alternate protein names: 5'-methylthioadenosine nucleosidase; S-adenosylhomocysteine nucleosidase

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTVGVFAALGEEVARVRECLGGVGTERAGLTFYVVSVGALQVVYVCGGVGKVNAALCTQLLISEFGARVLINTGIAGALD
ERLCVFDVLVSVDAVQHDVDVTAFGYQKGRIPRMDSVEWTANTALRYLVREAFDLCTRDPEWTEGACALSGSGDPPSRVS
RLVEGRVASGDLFVSDAQTRARIIREFGAHGVEMEGAAFAHVASVNGVPFVIIRCISDGAGAEQDVSMSYKEFSTRAARR
SALLTLRVLERLSALRTSVVASLFPMVVV

Sequences:

>Translated_269_residues
MTVGVFAALGEEVARVRECLGGVGTERAGLTFYVVSVGALQVVYVCGGVGKVNAALCTQLLISEFGARVLINTGIAGALD
ERLCVFDVLVSVDAVQHDVDVTAFGYQKGRIPRMDSVEWTANTALRYLVREAFDLCTRDPEWTEGACALSGSGDPPSRVS
RLVEGRVASGDLFVSDAQTRARIIREFGAHGVEMEGAAFAHVASVNGVPFVIIRCISDGAGAEQDVSMSYKEFSTRAARR
SALLTLRVLERLSALRTSVVASLFPMVVV
>Mature_268_residues
TVGVFAALGEEVARVRECLGGVGTERAGLTFYVVSVGALQVVYVCGGVGKVNAALCTQLLISEFGARVLINTGIAGALDE
RLCVFDVLVSVDAVQHDVDVTAFGYQKGRIPRMDSVEWTANTALRYLVREAFDLCTRDPEWTEGACALSGSGDPPSRVSR
LVEGRVASGDLFVSDAQTRARIIREFGAHGVEMEGAAFAHVASVNGVPFVIIRCISDGAGAEQDVSMSYKEFSTRAARRS
ALLTLRVLERLSALRTSVVASLFPMVVV

Specific function: Responsible for cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH)

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily

Homologues:

Organism=Escherichia coli, GI1786354, Length=255, Percent_Identity=32.5490196078431, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNN_TREPA (P96122)

Other databases:

- EMBL:   U55214
- EMBL:   AE000520
- PIR:   G71357
- RefSeq:   NP_218609.1
- ProteinModelPortal:   P96122
- SMR:   P96122
- IntAct:   P96122
- GeneID:   2611347
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0170
- NMPDR:   fig|243276.1.peg.168
- TIGR:   TP_0170
- HOGENOM:   HBG367723
- OMA:   LEHFPTM
- ProtClustDB:   CLSK2300152
- BioCyc:   TPAL243276:TP_0170-MONOMER
- BRENDA:   3.2.2.9
- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845
- PANTHER:   PTHR21234
- TIGRFAMs:   TIGR01704

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =3.2.2.9

Molecular weight: Translated: 28666; Mature: 28535

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVGVFAALGEEVARVRECLGGVGTERAGLTFYVVSVGALQVVYVCGGVGKVNAALCTQL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHH
LISEFGARVLINTGIAGALDERLCVFDVLVSVDAVQHDVDVTAFGYQKGRIPRMDSVEWT
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
ANTALRYLVREAFDLCTRDPEWTEGACALSGSGDPPSRVSRLVEGRVASGDLFVSDAQTR
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEEEECHHHH
ARIIREFGAHGVEMEGAAFAHVASVNGVPFVIIRCISDGAGAEQDVSMSYKEFSTRAARR
HHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
SALLTLRVLERLSALRTSVVASLFPMVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVGVFAALGEEVARVRECLGGVGTERAGLTFYVVSVGALQVVYVCGGVGKVNAALCTQL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHH
LISEFGARVLINTGIAGALDERLCVFDVLVSVDAVQHDVDVTAFGYQKGRIPRMDSVEWT
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
ANTALRYLVREAFDLCTRDPEWTEGACALSGSGDPPSRVSRLVEGRVASGDLFVSDAQTR
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEEEECHHHH
ARIIREFGAHGVEMEGAAFAHVASVNGVPFVIIRCISDGAGAEQDVSMSYKEFSTRAARR
HHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
SALLTLRVLERLSALRTSVVASLFPMVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9332349; 9665876