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The map label for this gene is dat [H]

Identifier: 189025375

GI number: 189025375

Start: 163092

End: 163625

Strand: Direct

Name: dat [H]

Synonym: TPASS_0141

Alternate gene names: 189025375

Gene position: 163092-163625 (Clockwise)

Preceding gene: 189025371

Following gene: 189025379

Centisome position: 14.31

GC content: 55.99

Gene sequence:

>534_bases
ATGTCTGTGCATCAGGGTGGGATACGTTTTCCCTTGCTGTATCTAGAGATGGAAACACCGCTCGGTCGTATGTGCATAGC
CGAGCGAGCGCACACTATTGTGTATGTGTGGTTCCATCGGGTTGGTCCAAGAGGATCTCCTGTATCTGGTGTGTGCGCGC
AGCACGTGTGCCCGGGTAAAACGGCGCTCTTGCGTGAGACGCAGTCCCAAATTCACGCTTACTTTTGTGGAGCGCTGCGC
GAGTTTTCAGTACCGGTCACGGTAGAGGGCACGCCTTTTATGCAACGCGTCTGGGGCGCCACGCGCAACATCGCGTACGG
CACGACAAAAAGCTATGCACAGCTTTCACAGGACATCGGCTGTCCACGCGCCGCACGCGCAGTTGGGCAGGCATTGCATC
GCAACCCGCTGCTTTTGCTTATTCCTTGCCATCGGGTAATAAGTGCGCGTGCATCAATCGGCGGTTTTGCGTACGGGAGT
GCTCTCAAGCATTTTTTGCTGCAGCAAGAGGCGGCTGTGTGCGCATGTGAATGA

Upstream 100 bases:

>100_bases
ATACGAACACGATAAGAACACATAGGTCGTAAAGATCAGATGTAGTTTTTCTTTGAAAAACGAGGATATCTGTGTTCGTG
GAGGGGGAGAGAGACAGGCA

Downstream 100 bases:

>100_bases
CGGTGTCCGCAAGGGCTGCCGCTTCTGTACTGTCGTGCGTGACGTAGACTGCAGTGTACCCAAGTGAACGCTGCAGCGCG
CGCAGCTCTCCTTGCAACGT

Product: methylated-DNA-protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MSVHQGGIRFPLLYLEMETPLGRMCIAERAHTIVYVWFHRVGPRGSPVSGVCAQHVCPGKTALLRETQSQIHAYFCGALR
EFSVPVTVEGTPFMQRVWGATRNIAYGTTKSYAQLSQDIGCPRAARAVGQALHRNPLLLLIPCHRVISARASIGGFAYGS
ALKHFLLQQEAAVCACE

Sequences:

>Translated_177_residues
MSVHQGGIRFPLLYLEMETPLGRMCIAERAHTIVYVWFHRVGPRGSPVSGVCAQHVCPGKTALLRETQSQIHAYFCGALR
EFSVPVTVEGTPFMQRVWGATRNIAYGTTKSYAQLSQDIGCPRAARAVGQALHRNPLLLLIPCHRVISARASIGGFAYGS
ALKHFLLQQEAAVCACE
>Mature_176_residues
SVHQGGIRFPLLYLEMETPLGRMCIAERAHTIVYVWFHRVGPRGSPVSGVCAQHVCPGKTALLRETQSQIHAYFCGALRE
FSVPVTVEGTPFMQRVWGATRNIAYGTTKSYAQLSQDIGCPRAARAVGQALHRNPLLLLIPCHRVISARASIGGFAYGSA
LKHFLLQQEAAVCACE

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=161, Percent_Identity=30.4347826086957, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1788542, Length=90, Percent_Identity=43.3333333333333, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1787596, Length=104, Percent_Identity=39.4230769230769, Blast_Score=81, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=42.6829268292683, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=42.6829268292683, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320001, Length=82, Percent_Identity=37.8048780487805, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19379; Mature: 19248

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVHQGGIRFPLLYLEMETPLGRMCIAERAHTIVYVWFHRVGPRGSPVSGVCAQHVCPGK
CCCCCCCCCCEEEEEEECCCCHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHCCCCCH
TALLRETQSQIHAYFCGALREFSVPVTVEGTPFMQRVWGATRNIAYGTTKSYAQLSQDIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
CPRAARAVGQALHRNPLLLLIPCHRVISARASIGGFAYGSALKHFLLQQEAAVCACE
CCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVHQGGIRFPLLYLEMETPLGRMCIAERAHTIVYVWFHRVGPRGSPVSGVCAQHVCPGK
CCCCCCCCCEEEEEEECCCCHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHCCCCCH
TALLRETQSQIHAYFCGALREFSVPVTVEGTPFMQRVWGATRNIAYGTTKSYAQLSQDIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
CPRAARAVGQALHRNPLLLLIPCHRVISARASIGGFAYGSALKHFLLQQEAAVCACE
CCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]