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The map label for this gene is gatA [H]

Identifier: 188586560

GI number: 188586560

Start: 2053400

End: 2054866

Strand: Reverse

Name: gatA [H]

Synonym: Nther_1946

Alternate gene names: 188586560

Gene position: 2054866-2053400 (Counterclockwise)

Preceding gene: 188586561

Following gene: 188586559

Centisome position: 64.91

GC content: 37.83

Gene sequence:

>1467_bases
GTGTATAGTCACTTTCTTAACAAATGGCAGCAGGATTACCTGTTGGAAACAACTGTTGATGAATTACAGGATTATATGGA
GAAAGGCGAGATTACTAGTCGTGATTTAGTTTTAATGTATCAAAATAGAATTGCATGTTTAGATCAAGATGGTCCTAAAA
TCAATTCAATTATAGAAATGAATCCTGAAGCTTTACAGATCGCTCAAGCCTTAGACCGGGAACGTAAATATAAAGGTCCC
AGAAGTAAATTGCATGGTATTCCTGTTCTTTTGAAGGATAATATTGACACTGAAGATAAAATGAGGACAAGTGCTGGATC
TATAGCATTGGAAGAACATGTGGCTAGAGAGGATGCCTTTATAGTTAAGAAACTTAGACAAGCTGGTGCTGTGATTCTAG
GTAAGGCAAATTTAACTGAATGGGCTAACTTTATGGCTGATAATATGCCTAATGGATACAGCTCTCGAGGTGGACAAGTT
TTAAATCCCTATGGACCTGGTGAATTGGATTGTGGTGGTTCTTCAAGTGGATCGGCCGCAGCCTTAGCCGCTAATTTTAC
AGTCTTGGCAATTGGGACAGAAACTAGTGGATCTATTTTGAGTCCAGCTAGTGATAATTCTGTGGTTGGGATAAAACCTA
CAGTAGGACTTTGGAGCAGGTCGGGAATTATACCTATATCCCATAGCCAAGATACTGCCGGACCTATGGCTCGAACAGTG
GAAGATGCAGCAATATTACTTGAGATACTATCAGGACCAGATGCTAAAGATCCGGTAACATTGACGAAAAAAGATGATAT
TGATGGTAGATACACAAATTATCTAAACGCTCACGGCCTTAAAGGTACACGTCTTGGCGTTTCACAGGAGTTTTTGAATT
TACTGGAGGATTCTGAACTGGACGTGATAGAAAAAGCTATTAAAAATCTGGAAAATTTAGGAGCACATATTGTATCAGGT
ATAACTTATCCACCTAAAATTGATTGGGACTTAAATGTCTTGCTTCACGAATTTAAAGTAGGTATAAACACTTATTTAAG
AAATACAGGGGATAGAGTACCAGTGAAGTCCTTGACAGAACTGATAAATTTCAATTATCGTAATAAAGAAATAGCTCTAA
AATATGGACAGGAACGGCTATTAAAATCTAATAAAACAAAAGGTACTCTTACTGAGCCTGAGTACTTAAAAAGCAGAGAA
CACGATTTGTTTTATTCCAAGGATAAGGGAATAGACGCGGTTATGGAAGAATATAATCTTGATGCGTTAATATTTCCGGC
CAATTTAGGGGCAAATATACCTGCTAGAGCAGGATATCCTTCTATTACCGTACCTGCAGGTTATAACAGTACGGGAAAAC
CCATTGGCTTGACATTTACAGCTAAAGCTTACGAAGAAGGTAAATTGATCAAGATTGCATATAGCTATGAACAAGCAGTA
AGTAATAGAGTCCCACCTCAGAAATAA

Upstream 100 bases:

>100_bases
ATTTAATGAGTCTTATTACTGTTAATCTTCAATAGAACGGATCATGCGAGGGTGTAACAGGGTGATTTCCTTAAAATTAT
CTTAATTAAGGAGGCTAAAA

Downstream 100 bases:

>100_bases
GTTGAAATTTTACAGTTATCAACATTCAACTTTAATACAGCCAAGTCAAAAAATAATATTGTCAATTTTGTTTGATTTAT
ATATAATCTAGTTTGTAATA

Product: amidase

Products: ADP; phosphate; Gln-tRNA(Gln); L-glutamate

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MYSHFLNKWQQDYLLETTVDELQDYMEKGEITSRDLVLMYQNRIACLDQDGPKINSIIEMNPEALQIAQALDRERKYKGP
RSKLHGIPVLLKDNIDTEDKMRTSAGSIALEEHVAREDAFIVKKLRQAGAVILGKANLTEWANFMADNMPNGYSSRGGQV
LNPYGPGELDCGGSSSGSAAALAANFTVLAIGTETSGSILSPASDNSVVGIKPTVGLWSRSGIIPISHSQDTAGPMARTV
EDAAILLEILSGPDAKDPVTLTKKDDIDGRYTNYLNAHGLKGTRLGVSQEFLNLLEDSELDVIEKAIKNLENLGAHIVSG
ITYPPKIDWDLNVLLHEFKVGINTYLRNTGDRVPVKSLTELINFNYRNKEIALKYGQERLLKSNKTKGTLTEPEYLKSRE
HDLFYSKDKGIDAVMEEYNLDALIFPANLGANIPARAGYPSITVPAGYNSTGKPIGLTFTAKAYEEGKLIKIAYSYEQAV
SNRVPPQK

Sequences:

>Translated_488_residues
MYSHFLNKWQQDYLLETTVDELQDYMEKGEITSRDLVLMYQNRIACLDQDGPKINSIIEMNPEALQIAQALDRERKYKGP
RSKLHGIPVLLKDNIDTEDKMRTSAGSIALEEHVAREDAFIVKKLRQAGAVILGKANLTEWANFMADNMPNGYSSRGGQV
LNPYGPGELDCGGSSSGSAAALAANFTVLAIGTETSGSILSPASDNSVVGIKPTVGLWSRSGIIPISHSQDTAGPMARTV
EDAAILLEILSGPDAKDPVTLTKKDDIDGRYTNYLNAHGLKGTRLGVSQEFLNLLEDSELDVIEKAIKNLENLGAHIVSG
ITYPPKIDWDLNVLLHEFKVGINTYLRNTGDRVPVKSLTELINFNYRNKEIALKYGQERLLKSNKTKGTLTEPEYLKSRE
HDLFYSKDKGIDAVMEEYNLDALIFPANLGANIPARAGYPSITVPAGYNSTGKPIGLTFTAKAYEEGKLIKIAYSYEQAV
SNRVPPQK
>Mature_488_residues
MYSHFLNKWQQDYLLETTVDELQDYMEKGEITSRDLVLMYQNRIACLDQDGPKINSIIEMNPEALQIAQALDRERKYKGP
RSKLHGIPVLLKDNIDTEDKMRTSAGSIALEEHVAREDAFIVKKLRQAGAVILGKANLTEWANFMADNMPNGYSSRGGQV
LNPYGPGELDCGGSSSGSAAALAANFTVLAIGTETSGSILSPASDNSVVGIKPTVGLWSRSGIIPISHSQDTAGPMARTV
EDAAILLEILSGPDAKDPVTLTKKDDIDGRYTNYLNAHGLKGTRLGVSQEFLNLLEDSELDVIEKAIKNLENLGAHIVSG
ITYPPKIDWDLNVLLHEFKVGINTYLRNTGDRVPVKSLTELINFNYRNKEIALKYGQERLLKSNKTKGTLTEPEYLKSRE
HDLFYSKDKGIDAVMEEYNLDALIFPANLGANIPARAGYPSITVPAGYNSTGKPIGLTFTAKAYEEGKLIKIAYSYEQAV
SNRVPPQK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=509, Percent_Identity=26.5225933202358, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI195972892, Length=496, Percent_Identity=26.008064516129, Blast_Score=100, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=510, Percent_Identity=25.0980392156863, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=448, Percent_Identity=25.6696428571429, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=245, Percent_Identity=26.1224489795918, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=210, Percent_Identity=31.4285714285714, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=243, Percent_Identity=36.2139917695473, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6319685, Length=438, Percent_Identity=24.2009132420091, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648113, Length=477, Percent_Identity=28.9308176100629, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24644968, Length=521, Percent_Identity=26.4875239923225, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI21356731, Length=504, Percent_Identity=25, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI161078093, Length=516, Percent_Identity=23.4496124031008, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.7

Molecular weight: Translated: 53670; Mature: 53670

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSHFLNKWQQDYLLETTVDELQDYMEKGEITSRDLVLMYQNRIACLDQDGPKINSIIEM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCCCCCCEEEC
NPEALQIAQALDRERKYKGPRSKLHGIPVLLKDNIDTEDKMRTSAGSIALEEHVAREDAF
CHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHH
IVKKLRQAGAVILGKANLTEWANFMADNMPNGYSSRGGQVLNPYGPGELDCGGSSSGSAA
HHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCE
ALAANFTVLAIGTETSGSILSPASDNSVVGIKPTVGLWSRSGIIPISHSQDTAGPMARTV
EEEECEEEEEEECCCCCCEECCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHH
EDAAILLEILSGPDAKDPVTLTKKDDIDGRYTNYLNAHGLKGTRLGVSQEFLNLLEDSEL
HHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCH
DVIEKAIKNLENLGAHIVSGITYPPKIDWDLNVLLHEFKVGINTYLRNTGDRVPVKSLTE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LINFNYRNKEIALKYGQERLLKSNKTKGTLTEPEYLKSREHDLFYSKDKGIDAVMEEYNL
HHCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEECCCCCHHHHHHHCCC
DALIFPANLGANIPARAGYPSITVPAGYNSTGKPIGLTFTAKAYEEGKLIKIAYSYEQAV
CEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEEECCCCCCCEEEEEECHHHHH
SNRVPPQK
HCCCCCCC
>Mature Secondary Structure
MYSHFLNKWQQDYLLETTVDELQDYMEKGEITSRDLVLMYQNRIACLDQDGPKINSIIEM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCCCCCCEEEC
NPEALQIAQALDRERKYKGPRSKLHGIPVLLKDNIDTEDKMRTSAGSIALEEHVAREDAF
CHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHH
IVKKLRQAGAVILGKANLTEWANFMADNMPNGYSSRGGQVLNPYGPGELDCGGSSSGSAA
HHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCE
ALAANFTVLAIGTETSGSILSPASDNSVVGIKPTVGLWSRSGIIPISHSQDTAGPMARTV
EEEECEEEEEEECCCCCCEECCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHH
EDAAILLEILSGPDAKDPVTLTKKDDIDGRYTNYLNAHGLKGTRLGVSQEFLNLLEDSEL
HHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCH
DVIEKAIKNLENLGAHIVSGITYPPKIDWDLNVLLHEFKVGINTYLRNTGDRVPVKSLTE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LINFNYRNKEIALKYGQERLLKSNKTKGTLTEPEYLKSREHDLFYSKDKGIDAVMEEYNL
HHCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEECCCCCHHHHHHHCCC
DALIFPANLGANIPARAGYPSITVPAGYNSTGKPIGLTFTAKAYEEGKLIKIAYSYEQAV
CEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEEECCCCCCCEEEEEECHHHHH
SNRVPPQK
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Glu-tRNA(Gln); L-glutamine

Specific reaction: ATP + Glu-tRNA(Gln) + L-glutamine = ADP + phosphate + Gln-tRNA(Gln) + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA