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The map label for this gene is yyaE [H]

Identifier: 188586535

GI number: 188586535

Start: 2016067

End: 2018076

Strand: Reverse

Name: yyaE [H]

Synonym: Nther_1921

Alternate gene names: 188586535

Gene position: 2018076-2016067 (Counterclockwise)

Preceding gene: 188586536

Following gene: 188586534

Centisome position: 63.75

GC content: 36.92

Gene sequence:

>2010_bases
TTGGTTCAAGTCTACAAAAGTACTTGTCCATTGGATTGCTGGGATGCTTGTAGCTTGGAAGTTACTGTAGATAAAGGTGA
AGTAATTAATATACAAGGGGACGGAGAGCACTCAATTACCCGAGGATTTATTTGTTCTAAGGGGAAAAAGCATCTGGAGC
GAACATATCGAGATGATAGACTGACTTCACCCATGGTTAAAACCCATGAGGATGAATGGCAGGAAATAACATGGGAGAAA
GCCTTTGAATTAATCACATCCAAAATATCAGCTATTCGCGAACAAGATAGTAAACGACTAATGTATTATACTGATTCGGG
TCATGGCGGTTTATTAAGCAGTATTGAAAATTATTTTTTTAATTTAATTGGTGATGTCACGATACCTGTAGGAAGTCTTT
GCTGGAGTGCTGGTATGGAAGCCCAAAATATAGATTTTGGGATGAGTCTTAGTCATGACTATAGTGATGTACTAAACTCG
AAAACCATTCTCCTATGGGGCAGAAACCCTGCAGATACGGCTATACATCAGATGAATTATGTACAAAAAGCGAGAAAAAC
AGGAACAAGGGTTATAGCCATAGATCCGAGAAAAACAAAGAGCGCCAGGCAAGCCGATGAGCATATTTCTATTAAACCCG
GTACTGACGGTGCCCTGGCACTAGCTATGGCCAACCATATTATTAAAAATAACCTTCAGGATAACTCTTTTATAGACAAT
CATATTAAAGGTTTTGAAGAGTTTAAGGAGTTGCTCACTTCTTATACAATTGATTGGGCTGAAAAGGAAACGGGTATAGA
CAGGAGAAAAATTGAGGAATTAGCAGAATTATATGCTACACAAAAGCCAGCTGCAATTATGATTGGATACGGTATACAAC
GCTATACAAATAGTGCCAATACTGTAAGAGCAATTGATGCCTTGGCTGCAATTACAGGTAATATTGGAATTGCCGGTGGT
GGTGCAAATTATGCCAATAAACAGGCAGTTAATTATATAGATGATTCAGTATTTGGTGGAGATATTGATAGGAACCATAG
AACATTTCCTAAAGCCAAGTTAGCTAACTTTATTTTAGAGGAAAATAATCCACCTATAGATATGATGTTTATTACCAAAT
CTAACCCTTTTCTACAAGCTCCCGATACCAATAGAATGTATCAGGCCTTTGAAAAGGTAGACTTTAAGGTAACTATAGAT
ATGTTTATGACAGATACAGCCAGACAGTCTGATTTAGTTTTGCCTTGCGCTAATTTCATGGAAAAAGAAGATTTGTATTT
TACTTCAATGGGACACAATTATGTTTCTTATGGTCCTAAAATAATTGAACCTGAATACGATATTAAAAACGAACTTGACA
TTTTCACAAGCTTGGCAGATAAACTTAATTTAACTGAATTTCCTGAACAGGAGGCTGAGCAGTGGTTAAAGCAAGCTATC
AAACCCTTGGAAAAGTATAAAGGAATTAGCTTGGAAAAGTTAAAAGAAAAGCCCCAACGGATTAATGAGCCGGATACAAT
ACCATGGGCTGATAAAAACTTTTTAACACCTAGTGGAAAATATGAACTTTACTCTAAAAAGGCCCAAGAATGGGGATTGG
GTCCAATACCCGGATATTCACAACCCTTTTCAACTTACACTGATTATCAAAACAACAATATGACGAATTATGATTTTTAC
TTTTTAACACCACATCATAGAGATTCGTTGCATTCCCAGCACTTTATCGACAGAAGCCAAGATGAACCAGGTTATTTATA
TATCAATCCCGAAATAGCTGCTAAGTATCGACTAAGAGAAAACTCAGTTATAAAGATCGCTTCTCCTAAAGGCACCTTAT
CTGTAAAAATAAAATTTGATCCAGAGCTAAGAACAGATACGGTTTATATGTATGAGGGCTTATGGATCGGAAAAGGTGGC
AGTGTAAATACTTTGATATCCGATGCTCTTTCAGATGCCGGGGACCAAGCAGCTCTATATGAAACAAAAATCAGATTTGT
AGTTGAGTAA

Upstream 100 bases:

>100_bases
ATTATGAAGGATTTGTCTTTATGTTTACAGAATAAATCTTATTACAAAACAATTATGAATAAACAGTTGAAAAACAACTG
GAAGAAATGGAGTGATTTAT

Downstream 100 bases:

>100_bases
ATTAAATAAAAATTTATTAGAGTTCATACTAATGAGTTTAAATAAATTAGATAGCATTAGACTTTTAGTAAGGAGGTGTT
AAAAATGTCGGAAAAAAAGT

Product: molybdopterin oxidoreductase

Products: CO2; NADH; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 669; Mature: 669

Protein sequence:

>669_residues
MVQVYKSTCPLDCWDACSLEVTVDKGEVINIQGDGEHSITRGFICSKGKKHLERTYRDDRLTSPMVKTHEDEWQEITWEK
AFELITSKISAIREQDSKRLMYYTDSGHGGLLSSIENYFFNLIGDVTIPVGSLCWSAGMEAQNIDFGMSLSHDYSDVLNS
KTILLWGRNPADTAIHQMNYVQKARKTGTRVIAIDPRKTKSARQADEHISIKPGTDGALALAMANHIIKNNLQDNSFIDN
HIKGFEEFKELLTSYTIDWAEKETGIDRRKIEELAELYATQKPAAIMIGYGIQRYTNSANTVRAIDALAAITGNIGIAGG
GANYANKQAVNYIDDSVFGGDIDRNHRTFPKAKLANFILEENNPPIDMMFITKSNPFLQAPDTNRMYQAFEKVDFKVTID
MFMTDTARQSDLVLPCANFMEKEDLYFTSMGHNYVSYGPKIIEPEYDIKNELDIFTSLADKLNLTEFPEQEAEQWLKQAI
KPLEKYKGISLEKLKEKPQRINEPDTIPWADKNFLTPSGKYELYSKKAQEWGLGPIPGYSQPFSTYTDYQNNNMTNYDFY
FLTPHHRDSLHSQHFIDRSQDEPGYLYINPEIAAKYRLRENSVIKIASPKGTLSVKIKFDPELRTDTVYMYEGLWIGKGG
SVNTLISDALSDAGDQAALYETKIRFVVE

Sequences:

>Translated_669_residues
MVQVYKSTCPLDCWDACSLEVTVDKGEVINIQGDGEHSITRGFICSKGKKHLERTYRDDRLTSPMVKTHEDEWQEITWEK
AFELITSKISAIREQDSKRLMYYTDSGHGGLLSSIENYFFNLIGDVTIPVGSLCWSAGMEAQNIDFGMSLSHDYSDVLNS
KTILLWGRNPADTAIHQMNYVQKARKTGTRVIAIDPRKTKSARQADEHISIKPGTDGALALAMANHIIKNNLQDNSFIDN
HIKGFEEFKELLTSYTIDWAEKETGIDRRKIEELAELYATQKPAAIMIGYGIQRYTNSANTVRAIDALAAITGNIGIAGG
GANYANKQAVNYIDDSVFGGDIDRNHRTFPKAKLANFILEENNPPIDMMFITKSNPFLQAPDTNRMYQAFEKVDFKVTID
MFMTDTARQSDLVLPCANFMEKEDLYFTSMGHNYVSYGPKIIEPEYDIKNELDIFTSLADKLNLTEFPEQEAEQWLKQAI
KPLEKYKGISLEKLKEKPQRINEPDTIPWADKNFLTPSGKYELYSKKAQEWGLGPIPGYSQPFSTYTDYQNNNMTNYDFY
FLTPHHRDSLHSQHFIDRSQDEPGYLYINPEIAAKYRLRENSVIKIASPKGTLSVKIKFDPELRTDTVYMYEGLWIGKGG
SVNTLISDALSDAGDQAALYETKIRFVVE
>Mature_669_residues
MVQVYKSTCPLDCWDACSLEVTVDKGEVINIQGDGEHSITRGFICSKGKKHLERTYRDDRLTSPMVKTHEDEWQEITWEK
AFELITSKISAIREQDSKRLMYYTDSGHGGLLSSIENYFFNLIGDVTIPVGSLCWSAGMEAQNIDFGMSLSHDYSDVLNS
KTILLWGRNPADTAIHQMNYVQKARKTGTRVIAIDPRKTKSARQADEHISIKPGTDGALALAMANHIIKNNLQDNSFIDN
HIKGFEEFKELLTSYTIDWAEKETGIDRRKIEELAELYATQKPAAIMIGYGIQRYTNSANTVRAIDALAAITGNIGIAGG
GANYANKQAVNYIDDSVFGGDIDRNHRTFPKAKLANFILEENNPPIDMMFITKSNPFLQAPDTNRMYQAFEKVDFKVTID
MFMTDTARQSDLVLPCANFMEKEDLYFTSMGHNYVSYGPKIIEPEYDIKNELDIFTSLADKLNLTEFPEQEAEQWLKQAI
KPLEKYKGISLEKLKEKPQRINEPDTIPWADKNFLTPSGKYELYSKKAQEWGLGPIPGYSQPFSTYTDYQNNNMTNYDFY
FLTPHHRDSLHSQHFIDRSQDEPGYLYINPEIAAKYRLRENSVIKIASPKGTLSVKIKFDPELRTDTVYMYEGLWIGKGG
SVNTLISDALSDAGDQAALYETKIRFVVE

Specific function: Decomposes Formic Acid To Hydrogen And Carbon Dioxide Under Anaerobic Conditions In The Absence Of Exogenous Electron Acceptors. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=700, Percent_Identity=24.7142857142857, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87081797, Length=752, Percent_Identity=25.3989361702128, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI171474008, Length=748, Percent_Identity=26.0695187165775, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1787870, Length=756, Percent_Identity=25.6613756613757, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI87081994, Length=780, Percent_Identity=23.974358974359, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI145693196, Length=728, Percent_Identity=23.2142857142857, Blast_Score=162, Evalue=8e-41,
Organism=Escherichia coli, GI1787231, Length=575, Percent_Identity=25.5652173913043, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI1788534, Length=516, Percent_Identity=23.062015503876, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.2.1.2 [C]

Molecular weight: Translated: 75974; Mature: 75974

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQVYKSTCPLDCWDACSLEVTVDKGEVINIQGDGEHSITRGFICSKGKKHLERTYRDDR
CEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHCHHHCCCHHHHHHHHHHHH
LTSPMVKTHEDEWQEITWEKAFELITSKISAIREQDSKRLMYYTDSGHGGLLSSIENYFF
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
NLIGDVTIPVGSLCWSAGMEAQNIDFGMSLSHDYSDVLNSKTILLWGRNPADTAIHQMNY
HHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHH
VQKARKTGTRVIAIDPRKTKSARQADEHISIKPGTDGALALAMANHIIKNNLQDNSFIDN
HHHHHHCCCEEEEECCCCCCHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHH
HIKGFEEFKELLTSYTIDWAEKETGIDRRKIEELAELYATQKPAAIMIGYGIQRYTNSAN
HHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCC
TVRAIDALAAITGNIGIAGGGANYANKQAVNYIDDSVFGGDIDRNHRTFPKAKLANFILE
HHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
ENNPPIDMMFITKSNPFLQAPDTNRMYQAFEKVDFKVTIDMFMTDTARQSDLVLPCANFM
CCCCCEEEEEEECCCCEEECCCCHHHHHHHHHCCEEEEEEEEECCCCCCCCEEECHHHHC
EKEDLYFTSMGHNYVSYGPKIIEPEYDIKNELDIFTSLADKLNLTEFPEQEAEQWLKQAI
CCCCEEEEECCCCHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KPLEKYKGISLEKLKEKPQRINEPDTIPWADKNFLTPSGKYELYSKKAQEWGLGPIPGYS
HHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
QPFSTYTDYQNNNMTNYDFYFLTPHHRDSLHSQHFIDRSQDEPGYLYINPEIAAKYRLRE
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCEEEEEEECC
NSVIKIASPKGTLSVKIKFDPELRTDTVYMYEGLWIGKGGSVNTLISDALSDAGDQAALY
CCEEEEECCCCEEEEEEEECCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEE
ETKIRFVVE
EEEEEEEEC
>Mature Secondary Structure
MVQVYKSTCPLDCWDACSLEVTVDKGEVINIQGDGEHSITRGFICSKGKKHLERTYRDDR
CEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHCHHHCCCHHHHHHHHHHHH
LTSPMVKTHEDEWQEITWEKAFELITSKISAIREQDSKRLMYYTDSGHGGLLSSIENYFF
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
NLIGDVTIPVGSLCWSAGMEAQNIDFGMSLSHDYSDVLNSKTILLWGRNPADTAIHQMNY
HHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHH
VQKARKTGTRVIAIDPRKTKSARQADEHISIKPGTDGALALAMANHIIKNNLQDNSFIDN
HHHHHHCCCEEEEECCCCCCHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHH
HIKGFEEFKELLTSYTIDWAEKETGIDRRKIEELAELYATQKPAAIMIGYGIQRYTNSAN
HHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCC
TVRAIDALAAITGNIGIAGGGANYANKQAVNYIDDSVFGGDIDRNHRTFPKAKLANFILE
HHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
ENNPPIDMMFITKSNPFLQAPDTNRMYQAFEKVDFKVTIDMFMTDTARQSDLVLPCANFM
CCCCCEEEEEEECCCCEEECCCCHHHHHHHHHCCEEEEEEEEECCCCCCCCEEECHHHHC
EKEDLYFTSMGHNYVSYGPKIIEPEYDIKNELDIFTSLADKLNLTEFPEQEAEQWLKQAI
CCCCEEEEECCCCHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KPLEKYKGISLEKLKEKPQRINEPDTIPWADKNFLTPSGKYELYSKKAQEWGLGPIPGYS
HHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
QPFSTYTDYQNNNMTNYDFYFLTPHHRDSLHSQHFIDRSQDEPGYLYINPEIAAKYRLRE
CCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEECCCEEEEEEECC
NSVIKIASPKGTLSVKIKFDPELRTDTVYMYEGLWIGKGGSVNTLISDALSDAGDQAALY
CCEEEEECCCCEEEEEEEECCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCEEEE
ETKIRFVVE
EEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: Fe; Mo; Se [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.19 {3-pyridinecarboxaldehyde-NAD+}} 0.032 {NAD+}} 1.16 {3-acetylpyridine-NAD+}} 0.54 {deamino-NAD+}} 0.17 {thio-NAD+}} [C]

Substrates: Formate; NAD(+) [C]

Specific reaction: Formate + NAD(+) = CO2 + NADH. formate = CO2 + H2 [C]

General reaction: Redox reaction [C]

Inhibitor: Azide; Br-; Cl-; CN-; F-; HCO3-; HCOS-; NO2-; NO3-; OCN-; SCN- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1552862 [H]