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The map label for this gene is yfiS [H]

Identifier: 188586452

GI number: 188586452

Start: 1912486

End: 1913733

Strand: Reverse

Name: yfiS [H]

Synonym: Nther_1837

Alternate gene names: 188586452

Gene position: 1913733-1912486 (Counterclockwise)

Preceding gene: 188586453

Following gene: 188586451

Centisome position: 60.45

GC content: 37.42

Gene sequence:

>1248_bases
ATGAAAGGTGTTGAGCCTGTGAAAGGTTTAGGTGGCTATAGCTGGGTACTTGAGAACAGGTTGTTTTACTGGTTTTGGTT
GGCTAAGGCCATAAGTTCATTGGGAGCAGTATTTTTTAGACTGACAATTTTAATTACTATTACAGAAATGACCGATTCGG
CCATGGCATTAGGTTTGGTTTTAGGTGTTCAGGCACTGCCCTCCTTATTTATGTCTCCTCTAGCCGGAGTATTGGTAGAT
CGCTTAAACAAAAAAATAGTTTTAATAGTTCTCGATATCTTAAGAGCATCTTTAATGCTTTTGGCAATTTTCATAGTAAA
TGATCTGATACCTGTGATAATAATAGTTGCCATAATGGGTGTATGTACAACAGTTAGACAGGCGACAGATATGGCAATAC
TTCCGGCTTTGGTTGAACAAAAAGATTATATGGCAGCTACAGGTCTGTTACGTGGAACATTACAAATTATGCAGTTAGTT
GGCCCAGGGATAGCCGGTATACTTATAGATATATTTGGACTGCAAACTGTTTTTGGTGTTAACTCTCTGGCATTTCTTTT
TTCTGGTTTGTTTTTATTGTTTTTGCCTATATTTTTAGAGCAGGATTCTCGGGAAACATTTAATTTGAAAAAAGAAATAA
CAGAAGGTCTAGTAGCTATTAAGGGTTCTCGAGTTTTGATTAGTATGTTGGCTTTATACTGCGCTGTCAGTATCTTTGCC
GGAGGAACAGGAGTACTAATGGTAGATTATATTCAAAATATCCTACAGGCAAGTCCCTATCAACTAGGAGTCGTACAAAG
CGTCTTGGCTTTAGGAGCTATTTTAGCTAATCTGGTAGCTGGTTACTTCGGTAATCAGGCTCCGCGGTTTCATTTGCTGT
TGGGAGCAACTTTTGGAATTGGTATTGTTAATTTAATTTTCTTTACTGATCCAGGTATGATTATTTTAGGAATCTGGGCT
TTTATTATAGGAGCTTGTGATGGTATGAATGAAGCGCCGTTCTATAGTCTCATTATTGATTATTCGCCAGATGAAGTAAG
AGGTCGTATCATGAGTTTTGTCAATGCTTTAATACGACTAACAGCTATTATCAGTTTAGGATTAGCAGGAATATTTGCAG
GATGGTTTGGATCAGCCAATGTTATCGGGGCAAGTGGTATAATTCTATTATTACTTGGAATGGTTATTTTAATGGGTGAT
GGACGAAAAGTACTATCTAGGAAGGATGAACAGTTAGATTCTAGATGA

Upstream 100 bases:

>100_bases
GGTAGAGTGGAAATAGAACAAACAGACGGATCCTTCGCATAATCCGTTCAATTGAAATTTTACAGTATCAACAACGAAAT
TTAACATCGCCTAAATAAAA

Downstream 100 bases:

>100_bases
ACAGTTTGAACAGTTATATTCTAAAGGAAAAGATAGGGGGGTGCAAATCCTGAAAAAATTTTTCAGGTAGATTATGAAAA
TTTTAGTAGATGCAGATAGT

Product: major facilitator superfamily MFS_1

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKGVEPVKGLGGYSWVLENRLFYWFWLAKAISSLGAVFFRLTILITITEMTDSAMALGLVLGVQALPSLFMSPLAGVLVD
RLNKKIVLIVLDILRASLMLLAIFIVNDLIPVIIIVAIMGVCTTVRQATDMAILPALVEQKDYMAATGLLRGTLQIMQLV
GPGIAGILIDIFGLQTVFGVNSLAFLFSGLFLLFLPIFLEQDSRETFNLKKEITEGLVAIKGSRVLISMLALYCAVSIFA
GGTGVLMVDYIQNILQASPYQLGVVQSVLALGAILANLVAGYFGNQAPRFHLLLGATFGIGIVNLIFFTDPGMIILGIWA
FIIGACDGMNEAPFYSLIIDYSPDEVRGRIMSFVNALIRLTAIISLGLAGIFAGWFGSANVIGASGIILLLLGMVILMGD
GRKVLSRKDEQLDSR

Sequences:

>Translated_415_residues
MKGVEPVKGLGGYSWVLENRLFYWFWLAKAISSLGAVFFRLTILITITEMTDSAMALGLVLGVQALPSLFMSPLAGVLVD
RLNKKIVLIVLDILRASLMLLAIFIVNDLIPVIIIVAIMGVCTTVRQATDMAILPALVEQKDYMAATGLLRGTLQIMQLV
GPGIAGILIDIFGLQTVFGVNSLAFLFSGLFLLFLPIFLEQDSRETFNLKKEITEGLVAIKGSRVLISMLALYCAVSIFA
GGTGVLMVDYIQNILQASPYQLGVVQSVLALGAILANLVAGYFGNQAPRFHLLLGATFGIGIVNLIFFTDPGMIILGIWA
FIIGACDGMNEAPFYSLIIDYSPDEVRGRIMSFVNALIRLTAIISLGLAGIFAGWFGSANVIGASGIILLLLGMVILMGD
GRKVLSRKDEQLDSR
>Mature_415_residues
MKGVEPVKGLGGYSWVLENRLFYWFWLAKAISSLGAVFFRLTILITITEMTDSAMALGLVLGVQALPSLFMSPLAGVLVD
RLNKKIVLIVLDILRASLMLLAIFIVNDLIPVIIIVAIMGVCTTVRQATDMAILPALVEQKDYMAATGLLRGTLQIMQLV
GPGIAGILIDIFGLQTVFGVNSLAFLFSGLFLLFLPIFLEQDSRETFNLKKEITEGLVAIKGSRVLISMLALYCAVSIFA
GGTGVLMVDYIQNILQASPYQLGVVQSVLALGAILANLVAGYFGNQAPRFHLLLGATFGIGIVNLIFFTDPGMIILGIWA
FIIGACDGMNEAPFYSLIIDYSPDEVRGRIMSFVNALIRLTAIISLGLAGIFAGWFGSANVIGASGIILLLLGMVILMGD
GRKVLSRKDEQLDSR

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44719; Mature: 44719

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]