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The map label for this gene is mleN [H]

Identifier: 188586449

GI number: 188586449

Start: 1909150

End: 1910541

Strand: Reverse

Name: mleN [H]

Synonym: Nther_1834

Alternate gene names: 188586449

Gene position: 1910541-1909150 (Counterclockwise)

Preceding gene: 188586450

Following gene: 188586448

Centisome position: 60.35

GC content: 38.22

Gene sequence:

>1392_bases
ATGAATAAGGAGCATGGGAGGTCCCCGTCATTTTTAGAAGCTCTTATCGGTCTAATTTTAATAATTACAGTGATAGTGGG
AACTATTAGAATCGAACTTGTTTTAGAGACGCCACTGGTATTAGGTTCAGTTACAGCAGCTTTATTTGGCTTGTATTTGG
GTTATAGTTGGGACAGTATTCAGGAAGGCATGATAGATGGTATTAGAAACGGATTGATTGCCTGTTTAATTTTAATCATA
GTCGGGATGGTAGTCGGGACTTGGATTCTTGGAGGCACAATTCAAACCTTAATTTACTATGGTTTACAGTTCTTATCACC
GACGATTTTTTTGCCGGCAACGTTTTTATTATGTGCTATCACTTCTCTATTTATAGGAAGTTCCTTTGGAACTATTGCAA
CTATGGGATTGGTTCTTTTAGGAGTAGGTGGAGGACTTGGTATCCCAGCGGGAATGACTGCAGGTGCCATCGCATCTGGC
GCAATTTTTGGTGATAAAATGAGTCCATTATCGGATTCAACGAATTTAGCATCAGCAATGACTGGAGCAAATCTATTTGA
TCATATTGGGTCAATGTTGTGGGTGACAGCTCCTGCCACAATTATTTCCTTGATCCTATATACTATTATTGGTCTGGGTC
AAAGTGGAGGAGCAGTAGACTTACAGGAAGTAAATACGATTTTGGAAACTTTACAATCCAATTTTAATATTACACTTTTA
ACTTTAATTCCACCTGTGTTAGTATTGATACTTTCAATTAAAAAAGTACCAGCTCTAGCTACTCTTATGATCAGCTTTTT
CGCTGGTTCTTTAATAGCGGTGATTACCCAGGGAGCAAGTTTGAGTTCTATCATTACAGTCTCTGCTTCAGGTTATGAAG
CAAGTACAGGAGTAGAATTTGTAGATGAATTATTAACCCATGGTGGCATTAATATGATGATGGGTACCGTAGCTATGATT
TTGGCAGGAACTGCCATGGGCGGTATATTAGAACGGTGTGGTGTTTTAAAAGTATTACTCAATGGAATGCTTCAATATGT
AAAAACACCCAGGGGATTAATTTTATCAACACTGGCTTCGGCTTATATTATGTTACTTGCATCAGCGGAAATGATGGTAT
CGATTATTGTACCTGGAAGAACTTTTCAGCCTGCTTATAATGATATGAATGTGGAATTGAGTGTCTTGTCAAGAACCCTG
GAAAGTGCAGCTACTTTAGGATGTATAGCCCTACCTTGGGGAGTTGCATCTGTTTATTTACAAGGAGTATTAGATGTGGG
TTTTGAGTTTATTCCCTATACCTTTATAAGCTTTTTAGCACCCGTCATTGTCATAATTTATGCAGTCACTAATACAGGTT
TTGGTCATGGTAAGAAACTGGAGAGACATTAA

Upstream 100 bases:

>100_bases
CTGTTGACGACATCATTATAGTTATATATCATGTACTGTATAATATTTAATCATTTAATACATAACAGTGTTAAAATGTT
TGATAGAAGGAGGTTTCAAT

Downstream 100 bases:

>100_bases
TCTTAAATCTAAAACGAGAATAGATCCTTGATAGAATTACACAATTCTGAGTCTATTTATCTGTAGAGATAAGACTTGGA
ATTGTGTTTTTTTTATTTTA

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNKEHGRSPSFLEALIGLILIITVIVGTIRIELVLETPLVLGSVTAALFGLYLGYSWDSIQEGMIDGIRNGLIACLILII
VGMVVGTWILGGTIQTLIYYGLQFLSPTIFLPATFLLCAITSLFIGSSFGTIATMGLVLLGVGGGLGIPAGMTAGAIASG
AIFGDKMSPLSDSTNLASAMTGANLFDHIGSMLWVTAPATIISLILYTIIGLGQSGGAVDLQEVNTILETLQSNFNITLL
TLIPPVLVLILSIKKVPALATLMISFFAGSLIAVITQGASLSSIITVSASGYEASTGVEFVDELLTHGGINMMMGTVAMI
LAGTAMGGILERCGVLKVLLNGMLQYVKTPRGLILSTLASAYIMLLASAEMMVSIIVPGRTFQPAYNDMNVELSVLSRTL
ESAATLGCIALPWGVASVYLQGVLDVGFEFIPYTFISFLAPVIVIIYAVTNTGFGHGKKLERH

Sequences:

>Translated_463_residues
MNKEHGRSPSFLEALIGLILIITVIVGTIRIELVLETPLVLGSVTAALFGLYLGYSWDSIQEGMIDGIRNGLIACLILII
VGMVVGTWILGGTIQTLIYYGLQFLSPTIFLPATFLLCAITSLFIGSSFGTIATMGLVLLGVGGGLGIPAGMTAGAIASG
AIFGDKMSPLSDSTNLASAMTGANLFDHIGSMLWVTAPATIISLILYTIIGLGQSGGAVDLQEVNTILETLQSNFNITLL
TLIPPVLVLILSIKKVPALATLMISFFAGSLIAVITQGASLSSIITVSASGYEASTGVEFVDELLTHGGINMMMGTVAMI
LAGTAMGGILERCGVLKVLLNGMLQYVKTPRGLILSTLASAYIMLLASAEMMVSIIVPGRTFQPAYNDMNVELSVLSRTL
ESAATLGCIALPWGVASVYLQGVLDVGFEFIPYTFISFLAPVIVIIYAVTNTGFGHGKKLERH
>Mature_463_residues
MNKEHGRSPSFLEALIGLILIITVIVGTIRIELVLETPLVLGSVTAALFGLYLGYSWDSIQEGMIDGIRNGLIACLILII
VGMVVGTWILGGTIQTLIYYGLQFLSPTIFLPATFLLCAITSLFIGSSFGTIATMGLVLLGVGGGLGIPAGMTAGAIASG
AIFGDKMSPLSDSTNLASAMTGANLFDHIGSMLWVTAPATIISLILYTIIGLGQSGGAVDLQEVNTILETLQSNFNITLL
TLIPPVLVLILSIKKVPALATLMISFFAGSLIAVITQGASLSSIITVSASGYEASTGVEFVDELLTHGGINMMMGTVAMI
LAGTAMGGILERCGVLKVLLNGMLQYVKTPRGLILSTLASAYIMLLASAEMMVSIIVPGRTFQPAYNDMNVELSVLSRTL
ESAATLGCIALPWGVASVYLQGVLDVGFEFIPYTFISFLAPVIVIIYAVTNTGFGHGKKLERH

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 48524; Mature: 48524

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEHGRSPSFLEALIGLILIITVIVGTIRIELVLETPLVLGSVTAALFGLYLGYSWDSI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHCCCHHHH
QEGMIDGIRNGLIACLILIIVGMVVGTWILGGTIQTLIYYGLQFLSPTIFLPATFLLCAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TSLFIGSSFGTIATMGLVLLGVGGGLGIPAGMTAGAIASGAIFGDKMSPLSDSTNLASAM
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHH
TGANLFDHIGSMLWVTAPATIISLILYTIIGLGQSGGAVDLQEVNTILETLQSNFNITLL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEH
TLIPPVLVLILSIKKVPALATLMISFFAGSLIAVITQGASLSSIITVSASGYEASTGVEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHH
VDELLTHGGINMMMGTVAMILAGTAMGGILERCGVLKVLLNGMLQYVKTPRGLILSTLAS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AYIMLLASAEMMVSIIVPGRTFQPAYNDMNVELSVLSRTLESAATLGCIALPWGVASVYL
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGVLDVGFEFIPYTFISFLAPVIVIIYAVTNTGFGHGKKLERH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNKEHGRSPSFLEALIGLILIITVIVGTIRIELVLETPLVLGSVTAALFGLYLGYSWDSI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHCCCHHHH
QEGMIDGIRNGLIACLILIIVGMVVGTWILGGTIQTLIYYGLQFLSPTIFLPATFLLCAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TSLFIGSSFGTIATMGLVLLGVGGGLGIPAGMTAGAIASGAIFGDKMSPLSDSTNLASAM
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHH
TGANLFDHIGSMLWVTAPATIISLILYTIIGLGQSGGAVDLQEVNTILETLQSNFNITLL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEH
TLIPPVLVLILSIKKVPALATLMISFFAGSLIAVITQGASLSSIITVSASGYEASTGVEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHH
VDELLTHGGINMMMGTVAMILAGTAMGGILERCGVLKVLLNGMLQYVKTPRGLILSTLAS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AYIMLLASAEMMVSIIVPGRTFQPAYNDMNVELSVLSRTLESAATLGCIALPWGVASVYL
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGVLDVGFEFIPYTFISFLAPVIVIIYAVTNTGFGHGKKLERH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]