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The map label for this gene is pfpI [H]

Identifier: 188586351

GI number: 188586351

Start: 1801182

End: 1801694

Strand: Reverse

Name: pfpI [H]

Synonym: Nther_1734

Alternate gene names: 188586351

Gene position: 1801694-1801182 (Counterclockwise)

Preceding gene: 188586352

Following gene: 188586350

Centisome position: 56.92

GC content: 34.7

Gene sequence:

>513_bases
ATGCAAGATAAAAAAGCTATTGTTTTATGTGCAAATATGTATGAAGAGAGAGAGTTATGGTATCCTTATTATCGACTTCA
AGAAGCTGGTTTAGAAGTAGAATTGGTTGGGGCTGAGGAAGGAACATATACAGGAAAAGCAGGTCTGCCATGCAAGGTCG
ACAAGCAAATATCCCAGATTAATGCTGAAGAAGTAGATGCTGTAATAATACCAGGTGGTTTTGCCCCAGATAGCTTAAGA
AGAAATCAACAAATTCTAAATGTAATTAAAAAAGTAAATAACAATGGAGGTTTAATTGCTGCTATCTGCCATGGTCCCTG
GCTATTAGTTTCTGCTGATATAATTTCTGGTAAACATATAACTTGTTTTTGGGCTATCATAGATGATGTTAAAAATGCAG
GTGCCCATTATGAAGATCGTGAAGTTATTAGAGATGGTAATATAGTTACTTCAAGGATTCCTCATGATTTACCGGAATTT
ATGAAAGAATGTTTAACAGTATTGCAAGATTAA

Upstream 100 bases:

>100_bases
AACCTTTTTAAACATAATTATTTGATAACCAGGAAAATGTAATATAATATCGAAAAATAATATTTAGAGAAACAAAATAA
TTTAGGAGGTAGTTATTAGA

Downstream 100 bases:

>100_bases
TCGAAACATGTGACAATTTTAGTTGTCATATGAATATGGGAAGATCTCTTTGTTTGAGATCTTCCTTTTTAGTGGCTTTA
AGTGAAATTGAAATTTTATT

Product: intracellular protease, PfpI family

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MQDKKAIVLCANMYEERELWYPYYRLQEAGLEVELVGAEEGTYTGKAGLPCKVDKQISQINAEEVDAVIIPGGFAPDSLR
RNQQILNVIKKVNNNGGLIAAICHGPWLLVSADIISGKHITCFWAIIDDVKNAGAHYEDREVIRDGNIVTSRIPHDLPEF
MKECLTVLQD

Sequences:

>Translated_170_residues
MQDKKAIVLCANMYEERELWYPYYRLQEAGLEVELVGAEEGTYTGKAGLPCKVDKQISQINAEEVDAVIIPGGFAPDSLR
RNQQILNVIKKVNNNGGLIAAICHGPWLLVSADIISGKHITCFWAIIDDVKNAGAHYEDREVIRDGNIVTSRIPHDLPEF
MKECLTVLQD
>Mature_170_residues
MQDKKAIVLCANMYEERELWYPYYRLQEAGLEVELVGAEEGTYTGKAGLPCKVDKQISQINAEEVDAVIIPGGFAPDSLR
RNQQILNVIKKVNNNGGLIAAICHGPWLLVSADIISGKHITCFWAIIDDVKNAGAHYEDREVIRDGNIVTSRIPHDLPEF
MKECLTVLQD

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Homo sapiens, GI183227678, Length=169, Percent_Identity=31.9526627218935, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI31543380, Length=169, Percent_Identity=31.9526627218935, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI87082219, Length=168, Percent_Identity=41.6666666666667, Blast_Score=131, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17558714, Length=168, Percent_Identity=27.3809523809524, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28571932, Length=165, Percent_Identity=29.6969696969697, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 18982; Mature: 18982

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDKKAIVLCANMYEERELWYPYYRLQEAGLEVELVGAEEGTYTGKAGLPCKVDKQISQI
CCCCCEEEEEECCHHHHHHHCHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHC
NAEEVDAVIIPGGFAPDSLRRNQQILNVIKKVNNNGGLIAAICHGPWLLVSADIISGKHI
CHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCCEE
TCFWAIIDDVKNAGAHYEDREVIRDGNIVTSRIPHDLPEFMKECLTVLQD
EEHHHHHHHHHHCCCCCCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDKKAIVLCANMYEERELWYPYYRLQEAGLEVELVGAEEGTYTGKAGLPCKVDKQISQI
CCCCCEEEEEECCHHHHHHHCHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHC
NAEEVDAVIIPGGFAPDSLRRNQQILNVIKKVNNNGGLIAAICHGPWLLVSADIISGKHI
CHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCCEE
TCFWAIIDDVKNAGAHYEDREVIRDGNIVTSRIPHDLPEFMKECLTVLQD
EEHHHHHHHHHHCCCCCCCHHHHCCCCEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]