Click here to switch to the map view.

The map label for this gene is opuAB [H]

Identifier: 188586238

GI number: 188586238

Start: 1699883

End: 1700719

Strand: Reverse

Name: opuAB [H]

Synonym: Nther_1621

Alternate gene names: 188586238

Gene position: 1700719-1699883 (Counterclockwise)

Preceding gene: 188586239

Following gene: 188586237

Centisome position: 53.73

GC content: 37.99

Gene sequence:

>837_bases
GTGATAACGATGATTCCTAGAATACCTATTGGAGACTGGTTTGAAAGTATTATAGACTGGATAGAAGAAGTTTTTGCTGT
CTTGTTTGATATTATTGATGTTGTGATTGAAGCTATCGTTGGATCTTTCATGTACGTTTTCGATACAATTTCAGTTCAAT
TATCACCATTCGTAGTAATTATTTTGCTAGCAGCTTTAGCATGGAGATTAAGTGGACTAGGAGTTGGAATTTTCACTTTA
CTGGGCTTTAGCCTTATAGTAAGTATGGACCTATGGGAACCGACTATGGTTACCTTTTCAATGATCTTAACTGCAGGTAT
TGTGGCTTTAGGACTTGGAATACCCATTGGTATTCTGGCATCAAAAAATGAAGTAGTAAATACAATTGTACGACCGGTTC
TCGACTTTATGCAGACCATGCCAGCTTTCGTCTATCTGATTCCGGCAGTTATATTCTTTAGCCTTGGTTTGGTTCCAGGA
GTTATAGCCACAGTTATCTTTGCTATGCCTCCTGCAATTAGGCTAACTAACTTTGGAATTAGGCAAGTGGATAAGGAAGT
AATTGAGGCAGGTCGAGCTTTTGGATCAACATCAAGTCAGCTATTATTTAAAGTACAACTGCCTTTGGCTGTCCCAACAA
TTATGGCTGGTGTTAACCAAACAATTATGCTGTCACTTTCCATGGTTGTTATCGCATCCATGATCGGTGCCGGTGGTTTA
GGTAATATTGTATTAAGAGGTATAACACAGCTGGACATAGGAACAGGTTTTGAAGGTGGTTTATCTGTAGTTATTTTAGC
AATATTCTTAGATAGAATCACTCAAAGTCTTCAATAA

Upstream 100 bases:

>100_bases
GAGTCAGACTACCCTATAGCAGTCGTAGATGAAGAAGATAAATTGCAAGGGCTGATTGTCAGGGTATCAGTTCTGGCCTC
ATTAGCTGAAGGAGAAGGAA

Downstream 100 bases:

>100_bases
TGTTTCAAAATGAGTGAAAACACCTTTTTAAAAGGTGAACATATAGTTTTCAATCAATCTATGAAGTTCAGTATCAAAAA
AAGGGAGGGGTTAGAATCAT

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MITMIPRIPIGDWFESIIDWIEEVFAVLFDIIDVVIEAIVGSFMYVFDTISVQLSPFVVIILLAALAWRLSGLGVGIFTL
LGFSLIVSMDLWEPTMVTFSMILTAGIVALGLGIPIGILASKNEVVNTIVRPVLDFMQTMPAFVYLIPAVIFFSLGLVPG
VIATVIFAMPPAIRLTNFGIRQVDKEVIEAGRAFGSTSSQLLFKVQLPLAVPTIMAGVNQTIMLSLSMVVIASMIGAGGL
GNIVLRGITQLDIGTGFEGGLSVVILAIFLDRITQSLQ

Sequences:

>Translated_278_residues
MITMIPRIPIGDWFESIIDWIEEVFAVLFDIIDVVIEAIVGSFMYVFDTISVQLSPFVVIILLAALAWRLSGLGVGIFTL
LGFSLIVSMDLWEPTMVTFSMILTAGIVALGLGIPIGILASKNEVVNTIVRPVLDFMQTMPAFVYLIPAVIFFSLGLVPG
VIATVIFAMPPAIRLTNFGIRQVDKEVIEAGRAFGSTSSQLLFKVQLPLAVPTIMAGVNQTIMLSLSMVVIASMIGAGGL
GNIVLRGITQLDIGTGFEGGLSVVILAIFLDRITQSLQ
>Mature_278_residues
MITMIPRIPIGDWFESIIDWIEEVFAVLFDIIDVVIEAIVGSFMYVFDTISVQLSPFVVIILLAALAWRLSGLGVGIFTL
LGFSLIVSMDLWEPTMVTFSMILTAGIVALGLGIPIGILASKNEVVNTIVRPVLDFMQTMPAFVYLIPAVIFFSLGLVPG
VIATVIFAMPPAIRLTNFGIRQVDKEVIEAGRAFGSTSSQLLFKVQLPLAVPTIMAGVNQTIMLSLSMVVIASMIGAGGL
GNIVLRGITQLDIGTGFEGGLSVVILAIFLDRITQSLQ

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=270, Percent_Identity=49.2592592592593, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI1788451, Length=189, Percent_Identity=29.1005291005291, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788449, Length=151, Percent_Identity=31.7880794701987, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786564, Length=169, Percent_Identity=27.2189349112426, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29877; Mature: 29877

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITMIPRIPIGDWFESIIDWIEEVFAVLFDIIDVVIEAIVGSFMYVFDTISVQLSPFVVI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLAALAWRLSGLGVGIFTLLGFSLIVSMDLWEPTMVTFSMILTAGIVALGLGIPIGILA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCC
SKNEVVNTIVRPVLDFMQTMPAFVYLIPAVIFFSLGLVPGVIATVIFAMPPAIRLTNFGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHH
RQVDKEVIEAGRAFGSTSSQLLFKVQLPLAVPTIMAGVNQTIMLSLSMVVIASMIGAGGL
HHHHHHHHHHHHHCCCCCHHEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCH
GNIVLRGITQLDIGTGFEGGLSVVILAIFLDRITQSLQ
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITMIPRIPIGDWFESIIDWIEEVFAVLFDIIDVVIEAIVGSFMYVFDTISVQLSPFVVI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLAALAWRLSGLGVGIFTLLGFSLIVSMDLWEPTMVTFSMILTAGIVALGLGIPIGILA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCC
SKNEVVNTIVRPVLDFMQTMPAFVYLIPAVIFFSLGLVPGVIATVIFAMPPAIRLTNFGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHH
RQVDKEVIEAGRAFGSTSSQLLFKVQLPLAVPTIMAGVNQTIMLSLSMVVIASMIGAGGL
HHHHHHHHHHHHHCCCCCHHEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCH
GNIVLRGITQLDIGTGFEGGLSVVILAIFLDRITQSLQ
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]