Click here to switch to the map view.

The map label for this gene is glyS

Identifier: 188585834

GI number: 188585834

Start: 1277147

End: 1279228

Strand: Direct

Name: glyS

Synonym: Nther_1208

Alternate gene names: 188585834

Gene position: 1277147-1279228 (Clockwise)

Preceding gene: 188585833

Following gene: 188585835

Centisome position: 40.35

GC content: 36.46

Gene sequence:

>2082_bases
ATGCATAATACATTACTATTCGAACTAGGAACTGAAGAATTACCAGCCAGATTTATTCCTGGTCTTATTAAACAGCTAGA
AGAAAATTCCGCTAATTTGCTCACAGAATACAGAATTGAATATGAAAACATAAAAGTAATGGCTACTCCCAGGAGATTAA
CCTTGCTTGTTAACGGGTTAGCGGACAAACAGCAAAGCAGTACCACAATGAAAAGGGGACCTGCTAAAGAGATTGCCTTT
GATGAAAATGGTGAACCAACCAAGGCTGCTATAGGTTTTGCCAGGGGTCAAAATATCGATCCAAAAGACTTGGAGATTAG
AGAAGATAATGGGAAAGAGTATGTATATGCTGTAGCTGAAATTGTAGGTGAAAATTGCCAGGATGTATTGCCAAAATTAC
TAGACGAGTTGATTACTAAATTTAATTTACCGGTTAAAATGTTTTGGAATAACAAACAGGATAAATTTCTACGACCAGTT
AGATGGTTGGTTTGCTTGTTTAATGATCAAGTGTTACCTTTAAACTTCGGAACTGTAGAGGCATCTAATATAAGCCGGGG
ACATCGGTTTCTATCCCAAGGTGATGTGATTATCAATTCAGCTGAAAATTATGAGGAAGTAATGCGTGATTCATGGGTGA
TGGTTGATCACAATGTAAGAGAGGAAACCATCAAAGCTCAGATTGAAGAACTGGCAGACCAAATGAATGCTAGTCCGGAA
ATGCCCCATGACTTATTGGAAGAAGTGAATTTTTTAGTTGAGTGGCCAACAGCTCTACTCGGGAATTTTGCAGAAGAGTT
TTTAGAAATACCCAAAGAGGCATTAATAACAAGCATGCAAGAACATCAAAAATATTTTGCCCTAGTAGACAAAGAGGATT
CTTCAAAATTACTTCCCTATTTTGTTGCAGTTAGAAATGGAGATGACCATGGGATCGATAAAGTAAAGGCAGGTAACGAG
CGAGTTCTCAGGGCTCGTCTTTCAGATGCCAGGTTCTTTTTTGAAGAAGATACAAAAACTTCTCTTGAATCAAAACGGGA
ACAGCTAAAATCTATAATTTACAAAGAACAGCTAGGAAGTGTTGACCAGAAAGTCACCCGCATGGAAGAGTTGGGTAAAA
CTCTATTAGACATCTTGGAATTATCAGAACCTGTACGATCTCTAACTTTGCGGGCAATTGCTCTATCTAAAGCGGATTTA
CCTACTAACATGGTATCAGAATTTCCTCATCTACAAGGGATTATGGGAGAGAAATATGCTAGGATACATGGAGAAGATGA
AAAAGTATGTCTAGGTATTTCGGAGCACTATCTTCCAAGACATACTGGTGATAAATTACCCCAAACAATGGTTGGGACTA
TTACCAGTATAGTTGATAAAGTTGATAATATTGCCAGTAGTTTTGCTATCGGAGAACGCCCCTCGGGTAGCCAAGACCCT
TATGCTTTGAGGCGACAAGCGCTAGGAATTGTCCATATAATTCTTGAAACAGAATTGGCTTTCTCACTAGAACAACTATT
CCACAGGGCTTTATCTCTCATACCCGAAAGTGCTTTAGTGGCACCAATTTCTAATATTCTTGATGATATTTTGGATTTCG
TTAAATTAAGAAGTAGAACAATTTTTAATGAACAGGGACTACCAATAGATATTATTGATAGCACTTTAGAGGTTGATAAA
GCAAATGTTTATAAAGCATATTTGAGAGCCAAAGTACTTAAAGATTACCGAGATTCTCAAGAATTAGAAGATGTTAGGAC
TGCCTATACTAGAGTTAAAAACCTAGCAAACAAAGCAAAGGGCGAAGATGTATATACTGAATTATTACAAGATGAAACAG
AAAAAATGTTATATCACAAATATCTGAATACCGAAGATAAATTATTATCTGATTTAGAACAGGATGAAATATCATCAGCG
ATTTCGGAACTGGCTGGTTTTAAAGATTATATTGATCAGTTTTTTGATGAAGTAATGGTTATGGTCGACGATAAACATTT
ACAAAACAATCGACTTAACCTGATTTACCACATAAAAGAAATGTATAGACAACTTGCTGATTTTTCAATTATCCAGGATT
AA

Upstream 100 bases:

>100_bases
TATATCGGCAGAGTTCGTGATTTGGCCAGAATATGTGCAAAGGCTTTTTTAAGCCAACGGGAAGAACTCAATTATCCTCT
TTTGAAGGAGGGTATCAGCT

Downstream 100 bases:

>100_bases
TTAAAGTTTTAAAAAGAGGCCAAATTTTTTGGTCTCTTTTTTTTAAAATTAGCAGGAAAAATTAAATATAAATAGTATAT
AATATATTGGCGTTTATTTT

Product: glycyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MHNTLLFELGTEELPARFIPGLIKQLEENSANLLTEYRIEYENIKVMATPRRLTLLVNGLADKQQSSTTMKRGPAKEIAF
DENGEPTKAAIGFARGQNIDPKDLEIREDNGKEYVYAVAEIVGENCQDVLPKLLDELITKFNLPVKMFWNNKQDKFLRPV
RWLVCLFNDQVLPLNFGTVEASNISRGHRFLSQGDVIINSAENYEEVMRDSWVMVDHNVREETIKAQIEELADQMNASPE
MPHDLLEEVNFLVEWPTALLGNFAEEFLEIPKEALITSMQEHQKYFALVDKEDSSKLLPYFVAVRNGDDHGIDKVKAGNE
RVLRARLSDARFFFEEDTKTSLESKREQLKSIIYKEQLGSVDQKVTRMEELGKTLLDILELSEPVRSLTLRAIALSKADL
PTNMVSEFPHLQGIMGEKYARIHGEDEKVCLGISEHYLPRHTGDKLPQTMVGTITSIVDKVDNIASSFAIGERPSGSQDP
YALRRQALGIVHIILETELAFSLEQLFHRALSLIPESALVAPISNILDDILDFVKLRSRTIFNEQGLPIDIIDSTLEVDK
ANVYKAYLRAKVLKDYRDSQELEDVRTAYTRVKNLANKAKGEDVYTELLQDETEKMLYHKYLNTEDKLLSDLEQDEISSA
ISELAGFKDYIDQFFDEVMVMVDDKHLQNNRLNLIYHIKEMYRQLADFSIIQD

Sequences:

>Translated_693_residues
MHNTLLFELGTEELPARFIPGLIKQLEENSANLLTEYRIEYENIKVMATPRRLTLLVNGLADKQQSSTTMKRGPAKEIAF
DENGEPTKAAIGFARGQNIDPKDLEIREDNGKEYVYAVAEIVGENCQDVLPKLLDELITKFNLPVKMFWNNKQDKFLRPV
RWLVCLFNDQVLPLNFGTVEASNISRGHRFLSQGDVIINSAENYEEVMRDSWVMVDHNVREETIKAQIEELADQMNASPE
MPHDLLEEVNFLVEWPTALLGNFAEEFLEIPKEALITSMQEHQKYFALVDKEDSSKLLPYFVAVRNGDDHGIDKVKAGNE
RVLRARLSDARFFFEEDTKTSLESKREQLKSIIYKEQLGSVDQKVTRMEELGKTLLDILELSEPVRSLTLRAIALSKADL
PTNMVSEFPHLQGIMGEKYARIHGEDEKVCLGISEHYLPRHTGDKLPQTMVGTITSIVDKVDNIASSFAIGERPSGSQDP
YALRRQALGIVHIILETELAFSLEQLFHRALSLIPESALVAPISNILDDILDFVKLRSRTIFNEQGLPIDIIDSTLEVDK
ANVYKAYLRAKVLKDYRDSQELEDVRTAYTRVKNLANKAKGEDVYTELLQDETEKMLYHKYLNTEDKLLSDLEQDEISSA
ISELAGFKDYIDQFFDEVMVMVDDKHLQNNRLNLIYHIKEMYRQLADFSIIQD
>Mature_693_residues
MHNTLLFELGTEELPARFIPGLIKQLEENSANLLTEYRIEYENIKVMATPRRLTLLVNGLADKQQSSTTMKRGPAKEIAF
DENGEPTKAAIGFARGQNIDPKDLEIREDNGKEYVYAVAEIVGENCQDVLPKLLDELITKFNLPVKMFWNNKQDKFLRPV
RWLVCLFNDQVLPLNFGTVEASNISRGHRFLSQGDVIINSAENYEEVMRDSWVMVDHNVREETIKAQIEELADQMNASPE
MPHDLLEEVNFLVEWPTALLGNFAEEFLEIPKEALITSMQEHQKYFALVDKEDSSKLLPYFVAVRNGDDHGIDKVKAGNE
RVLRARLSDARFFFEEDTKTSLESKREQLKSIIYKEQLGSVDQKVTRMEELGKTLLDILELSEPVRSLTLRAIALSKADL
PTNMVSEFPHLQGIMGEKYARIHGEDEKVCLGISEHYLPRHTGDKLPQTMVGTITSIVDKVDNIASSFAIGERPSGSQDP
YALRRQALGIVHIILETELAFSLEQLFHRALSLIPESALVAPISNILDDILDFVKLRSRTIFNEQGLPIDIIDSTLEVDK
ANVYKAYLRAKVLKDYRDSQELEDVRTAYTRVKNLANKAKGEDVYTELLQDETEKMLYHKYLNTEDKLLSDLEQDEISSA
ISELAGFKDYIDQFFDEVMVMVDDKHLQNNRLNLIYHIKEMYRQLADFSIIQD

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=693, Percent_Identity=35.4978354978355, Blast_Score=422, Evalue=1e-119,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_NATTJ (B2A1Y6)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917379.1
- ProteinModelPortal:   B2A1Y6
- GeneID:   6316717
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1208
- HOGENOM:   HBG586599
- OMA:   SNITYGH
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 79271; Mature: 79271

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNTLLFELGTEELPARFIPGLIKQLEENSANLLTEYRIEYENIKVMATPRRLTLLVNGL
CCCCEEEECCCHHCCHHHHHHHHHHHHCCCHHHHHHHEEEECCEEEEECCHHHHHHHHHH
ADKQQSSTTMKRGPAKEIAFDENGEPTKAAIGFARGQNIDPKDLEIREDNGKEYVYAVAE
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
IVGENCQDVLPKLLDELITKFNLPVKMFWNNKQDKFLRPVRWLVCLFNDQVLPLNFGTVE
HHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCEE
ASNISRGHRFLSQGDVIINSAENYEEVMRDSWVMVDHNVREETIKAQIEELADQMNASPE
CCCCHHHHHHHHCCCEEEECHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCC
MPHDLLEEVNFLVEWPTALLGNFAEEFLEIPKEALITSMQEHQKYFALVDKEDSSKLLPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCCEE
FVAVRNGDDHGIDKVKAGNERVLRARLSDARFFFEEDTKTSLESKREQLKSIIYKEQLGS
EEEEECCCCCCCCHHCCCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCH
VDQKVTRMEELGKTLLDILELSEPVRSLTLRAIALSKADLPTNMVSEFPHLQGIMGEKYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHCCHHH
RIHGEDEKVCLGISEHYLPRHTGDKLPQTMVGTITSIVDKVDNIASSFAIGERPSGSQDP
HHCCCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
YALRRQALGIVHIILETELAFSLEQLFHRALSLIPESALVAPISNILDDILDFVKLRSRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IFNEQGLPIDIIDSTLEVDKANVYKAYLRAKVLKDYRDSQELEDVRTAYTRVKNLANKAK
HCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
GEDVYTELLQDETEKMLYHKYLNTEDKLLSDLEQDEISSAISELAGFKDYIDQFFDEVMV
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVDDKHLQNNRLNLIYHIKEMYRQLADFSIIQD
HHCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHNTLLFELGTEELPARFIPGLIKQLEENSANLLTEYRIEYENIKVMATPRRLTLLVNGL
CCCCEEEECCCHHCCHHHHHHHHHHHHCCCHHHHHHHEEEECCEEEEECCHHHHHHHHHH
ADKQQSSTTMKRGPAKEIAFDENGEPTKAAIGFARGQNIDPKDLEIREDNGKEYVYAVAE
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
IVGENCQDVLPKLLDELITKFNLPVKMFWNNKQDKFLRPVRWLVCLFNDQVLPLNFGTVE
HHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCEE
ASNISRGHRFLSQGDVIINSAENYEEVMRDSWVMVDHNVREETIKAQIEELADQMNASPE
CCCCHHHHHHHHCCCEEEECHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCCC
MPHDLLEEVNFLVEWPTALLGNFAEEFLEIPKEALITSMQEHQKYFALVDKEDSSKLLPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCCEE
FVAVRNGDDHGIDKVKAGNERVLRARLSDARFFFEEDTKTSLESKREQLKSIIYKEQLGS
EEEEECCCCCCCCHHCCCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHCH
VDQKVTRMEELGKTLLDILELSEPVRSLTLRAIALSKADLPTNMVSEFPHLQGIMGEKYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHHHHCCHHH
RIHGEDEKVCLGISEHYLPRHTGDKLPQTMVGTITSIVDKVDNIASSFAIGERPSGSQDP
HHCCCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
YALRRQALGIVHIILETELAFSLEQLFHRALSLIPESALVAPISNILDDILDFVKLRSRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IFNEQGLPIDIIDSTLEVDKANVYKAYLRAKVLKDYRDSQELEDVRTAYTRVKNLANKAK
HCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
GEDVYTELLQDETEKMLYHKYLNTEDKLLSDLEQDEISSAISELAGFKDYIDQFFDEVMV
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVDDKHLQNNRLNLIYHIKEMYRQLADFSIIQD
HHCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA