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The map label for this gene is lepA [H]

Identifier: 188585805

GI number: 188585805

Start: 1249556

End: 1251355

Strand: Direct

Name: lepA [H]

Synonym: Nther_1179

Alternate gene names: 188585805

Gene position: 1249556-1251355 (Clockwise)

Preceding gene: 188585804

Following gene: 188585806

Centisome position: 39.47

GC content: 38.06

Gene sequence:

>1800_bases
ATGACACAAGAACGAATTAGAAACTTTTCGATCATTGCACATATCGATCATGGTAAATCAACACTAGCTGATCGGTTGTT
AGAATATACAAAAACCCTAACAGAACGAGAGATGCAGGAACAATTTCTTGACAATATGGATTTGGAAAGAGAACGAGGTA
TAACCATTAAACTGCAAGCGGTTACAATGAACTACCAGGCAAAGGATGGGAAAGAATATTATCTTAATGTAATTGACACT
CCTGGTCATGTTGATTTCACCTATGAAGTTTCTCGCAGTTTAAAAGCATGTGAGGGTGTATTATTAGTTGTAGATGCTGC
TCAGGGCATTGAAGCTCAAACCCTTGCGAATATTTATTTAGCTCTTGAACAAAACTTAGAAATTATACCAGTGATTAATA
AGATTGATTTACCTAATGCTGATGTAGAAAAAGTTACTAGAGAATTGGAAGAAATCGTAGGGATTGACGAAGATGAAGTT
ATTTTAGCTTCAGCTAAAGATGGTACAGGAATTGAGGACATTTTAGAAGCTATCGTGGAACGAATTCCGGCTCCCGATGA
TAAAGAAGATGACCATTTAAAAGCCCTAATTTTTGATTCTTATTACGACACCTATCGGGGAGTGATCACTTATCTTAGAG
TGTTTTCTGGTCAATTGGTTCCCGGAATGGAAATAAAGATGATGCAAACCGGAAAAAAATTCGAGGCCTCGGAAATTGGC
ATATTAAATCCGGGCCCTAAAAAAGTGGATGCCTTAAATGCTGGGGAAGTTGGATTTATGGCTGCCAATATAAAAGATGT
AAAGGATAGCCGGGTCGGAGATACTATTACTTCATATAAGAAACCAGCTCCAGAGCCTTTAGCAGGCTATAAGCAAGTAA
AACCAATGGTCTACTGTGGCTTGTTTCCTGCTGACGGAGAAGATTATGAAGATCTAAGGGATGCTTTGGATAAACTACAG
TTAAATGACGCTTCATTAGTATATGAACAAGAAAATTCCGCTGCCTTAGGATTTGGTTTCAGATGTGGATTTTTAGGACT
ACTTCATATGGAGATAATTCAAGAAAGGCTTGAAAGAGAATATGGGATTTCGCTAGTTACAACGGCTCCCAATGTTGTAT
ATAGAGTAGAACTAGTCGAAGGAGAAGAAATAACAATTGAAAATCCCACGGATCTACCAGAGAGAAACGAAATTAACGCA
ATCTATGAACCAGTAGTAAGAGCTAATTTTATGGTGCCGCAGGACTATATTGGCCCAGTCATGGAATTATGTGAAGAAAA
AAGAGGGGAATATGAAACCTTGGAATACTTGGATTCATTGCGGGTACTGCTCAAGTACAAGCTTCCCTTAAGTGAAATTG
TTTTTGATTTCTTTGATCTTCTTAAGAGTAAGACCAGGGGTTATGCTTCCTTTGATTACGAGATCATTGGTTACGAGGGA
GCGGATTTAGTTAAAGTGGATATCTTGGTGAACAAGGAACCTGTGGATGCATTATCCTTTATAGTTCATCAAGATAAAGC
TTATTATAAAGGAAGACAGCTGGCAGGTAAACTAAGAGAAATGCTGCCAAGACAAATGTTCGATGTTCCAGTACAAGCAG
CTATCGGTAATAAGGTAATTGCCAGGGAAACGGTGAAAGCAGTCAAAAAGAATGTCTTGGCAAAATGTTACGGTGGAGAT
ATCACTCGTAAGAGAAAATTATTAGAAAAACAAAAAGAAGGGAAACAGAGAATGAAACAAGTCGGGAAGGTAGATATACC
TCAAGAAGCCTTTATGGCGGTGTTGAGCATTGATAAATAA

Upstream 100 bases:

>100_bases
AGCTGAAGCTCTGAAAAAGGCGGCATTTGCTGGTGATTGACGACTTGTCACCCTCATGTCCAGGTGTTATAATAAGGTGA
GATAAAAGGGGGACCAGAAT

Downstream 100 bases:

>100_bases
AACGGATTACAAAAAGGGACTGTACATTCATGTCCCTTTTTGTTTATCTAAATGTAAATACTGCGATTTTAATAGTAGAG
AACTTCAATCCCAGGATGAA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTQERIRNFSIIAHIDHGKSTLADRLLEYTKTLTEREMQEQFLDNMDLERERGITIKLQAVTMNYQAKDGKEYYLNVIDT
PGHVDFTYEVSRSLKACEGVLLVVDAAQGIEAQTLANIYLALEQNLEIIPVINKIDLPNADVEKVTRELEEIVGIDEDEV
ILASAKDGTGIEDILEAIVERIPAPDDKEDDHLKALIFDSYYDTYRGVITYLRVFSGQLVPGMEIKMMQTGKKFEASEIG
ILNPGPKKVDALNAGEVGFMAANIKDVKDSRVGDTITSYKKPAPEPLAGYKQVKPMVYCGLFPADGEDYEDLRDALDKLQ
LNDASLVYEQENSAALGFGFRCGFLGLLHMEIIQERLEREYGISLVTTAPNVVYRVELVEGEEITIENPTDLPERNEINA
IYEPVVRANFMVPQDYIGPVMELCEEKRGEYETLEYLDSLRVLLKYKLPLSEIVFDFFDLLKSKTRGYASFDYEIIGYEG
ADLVKVDILVNKEPVDALSFIVHQDKAYYKGRQLAGKLREMLPRQMFDVPVQAAIGNKVIARETVKAVKKNVLAKCYGGD
ITRKRKLLEKQKEGKQRMKQVGKVDIPQEAFMAVLSIDK

Sequences:

>Translated_599_residues
MTQERIRNFSIIAHIDHGKSTLADRLLEYTKTLTEREMQEQFLDNMDLERERGITIKLQAVTMNYQAKDGKEYYLNVIDT
PGHVDFTYEVSRSLKACEGVLLVVDAAQGIEAQTLANIYLALEQNLEIIPVINKIDLPNADVEKVTRELEEIVGIDEDEV
ILASAKDGTGIEDILEAIVERIPAPDDKEDDHLKALIFDSYYDTYRGVITYLRVFSGQLVPGMEIKMMQTGKKFEASEIG
ILNPGPKKVDALNAGEVGFMAANIKDVKDSRVGDTITSYKKPAPEPLAGYKQVKPMVYCGLFPADGEDYEDLRDALDKLQ
LNDASLVYEQENSAALGFGFRCGFLGLLHMEIIQERLEREYGISLVTTAPNVVYRVELVEGEEITIENPTDLPERNEINA
IYEPVVRANFMVPQDYIGPVMELCEEKRGEYETLEYLDSLRVLLKYKLPLSEIVFDFFDLLKSKTRGYASFDYEIIGYEG
ADLVKVDILVNKEPVDALSFIVHQDKAYYKGRQLAGKLREMLPRQMFDVPVQAAIGNKVIARETVKAVKKNVLAKCYGGD
ITRKRKLLEKQKEGKQRMKQVGKVDIPQEAFMAVLSIDK
>Mature_598_residues
TQERIRNFSIIAHIDHGKSTLADRLLEYTKTLTEREMQEQFLDNMDLERERGITIKLQAVTMNYQAKDGKEYYLNVIDTP
GHVDFTYEVSRSLKACEGVLLVVDAAQGIEAQTLANIYLALEQNLEIIPVINKIDLPNADVEKVTRELEEIVGIDEDEVI
LASAKDGTGIEDILEAIVERIPAPDDKEDDHLKALIFDSYYDTYRGVITYLRVFSGQLVPGMEIKMMQTGKKFEASEIGI
LNPGPKKVDALNAGEVGFMAANIKDVKDSRVGDTITSYKKPAPEPLAGYKQVKPMVYCGLFPADGEDYEDLRDALDKLQL
NDASLVYEQENSAALGFGFRCGFLGLLHMEIIQERLEREYGISLVTTAPNVVYRVELVEGEEITIENPTDLPERNEINAI
YEPVVRANFMVPQDYIGPVMELCEEKRGEYETLEYLDSLRVLLKYKLPLSEIVFDFFDLLKSKTRGYASFDYEIIGYEGA
DLVKVDILVNKEPVDALSFIVHQDKAYYKGRQLAGKLREMLPRQMFDVPVQAAIGNKVIARETVKAVKKNVLAKCYGGDI
TRKRKLLEKQKEGKQRMKQVGKVDIPQEAFMAVLSIDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=600, Percent_Identity=51.3333333333333, Blast_Score=646, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=43.1818181818182, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI25306283, Length=150, Percent_Identity=43.3333333333333, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI25306287, Length=150, Percent_Identity=42.6666666666667, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI19923640, Length=150, Percent_Identity=43.3333333333333, Blast_Score=109, Evalue=9e-24,
Organism=Homo sapiens, GI4503483, Length=143, Percent_Identity=39.1608391608392, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI18390331, Length=184, Percent_Identity=34.2391304347826, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI217272892, Length=136, Percent_Identity=37.5, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI217272894, Length=136, Percent_Identity=37.5, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=39.4495412844037, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=39.4495412844037, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=39.4495412844037, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI94966752, Length=66, Percent_Identity=48.4848484848485, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI53729339, Length=220, Percent_Identity=28.1818181818182, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=220, Percent_Identity=28.1818181818182, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=56.6889632107023, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=508, Percent_Identity=30.511811023622, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1789738, Length=179, Percent_Identity=35.195530726257, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1790835, Length=137, Percent_Identity=30.6569343065693, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789559, Length=223, Percent_Identity=29.1479820627803, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=614, Percent_Identity=41.85667752443, Blast_Score=503, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17533571, Length=184, Percent_Identity=35.8695652173913, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=37.9310344827586, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=154, Percent_Identity=34.4155844155844, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=158, Percent_Identity=34.1772151898734, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=36.5671641791045, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=36.5671641791045, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25141371, Length=292, Percent_Identity=27.3972602739726, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=152, Percent_Identity=34.2105263157895, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=142, Percent_Identity=30.2816901408451, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17552884, Length=361, Percent_Identity=26.8698060941828, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17569207, Length=361, Percent_Identity=26.8698060941828, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI32566303, Length=332, Percent_Identity=27.710843373494, Blast_Score=65, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323320, Length=600, Percent_Identity=45.8333333333333, Blast_Score=537, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6323098, Length=202, Percent_Identity=34.1584158415842, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=39.8601398601399, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=39.8601398601399, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=114, Percent_Identity=40.3508771929825, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=36.1702127659575, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=244, Percent_Identity=29.5081967213115, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6322675, Length=141, Percent_Identity=31.2056737588652, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI78706572, Length=603, Percent_Identity=47.0978441127695, Blast_Score=578, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24582462, Length=154, Percent_Identity=40.2597402597403, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=39.568345323741, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=41.7218543046358, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585711, Length=188, Percent_Identity=34.0425531914894, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585713, Length=188, Percent_Identity=34.0425531914894, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585709, Length=188, Percent_Identity=34.0425531914894, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21357743, Length=166, Percent_Identity=35.5421686746988, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28572034, Length=229, Percent_Identity=30.5676855895196, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281363316, Length=255, Percent_Identity=27.4509803921569, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17864358, Length=255, Percent_Identity=27.4509803921569, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67707; Mature: 67575

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQERIRNFSIIAHIDHGKSTLADRLLEYTKTLTEREMQEQFLDNMDLERERGITIKLQA
CCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEE
VTMNYQAKDGKEYYLNVIDTPGHVDFTYEVSRSLKACEGVLLVVDAAQGIEAQTLANIYL
EEEEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
ALEQNLEIIPVINKIDLPNADVEKVTRELEEIVGIDEDEVILASAKDGTGIEDILEAIVE
HHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHH
RIPAPDDKEDDHLKALIFDSYYDTYRGVITYLRVFSGQLVPGMEIKMMQTGKKFEASEIG
HCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCC
ILNPGPKKVDALNAGEVGFMAANIKDVKDSRVGDTITSYKKPAPEPLAGYKQVKPMVYCG
CCCCCCCCCCCCCCCCCEEEECCCHHHCCCCCCCHHHHCCCCCCCCCCCHHHCCCEEEEE
LFPADGEDYEDLRDALDKLQLNDASLVYEQENSAALGFGFRCGFLGLLHMEIIQERLERE
ECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
YGISLVTTAPNVVYRVELVEGEEITIENPTDLPERNEINAIYEPVVRANFMVPQDYIGPV
HCCEEEEECCCEEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHH
MELCEEKRGEYETLEYLDSLRVLLKYKLPLSEIVFDFFDLLKSKTRGYASFDYEIIGYEG
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECEEEEECCC
ADLVKVDILVNKEPVDALSFIVHQDKAYYKGRQLAGKLREMLPRQMFDVPVQAAIGNKVI
CCEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHH
ARETVKAVKKNVLAKCYGGDITRKRKLLEKQKEGKQRMKQVGKVDIPQEAFMAVLSIDK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TQERIRNFSIIAHIDHGKSTLADRLLEYTKTLTEREMQEQFLDNMDLERERGITIKLQA
CHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEE
VTMNYQAKDGKEYYLNVIDTPGHVDFTYEVSRSLKACEGVLLVVDAAQGIEAQTLANIYL
EEEEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
ALEQNLEIIPVINKIDLPNADVEKVTRELEEIVGIDEDEVILASAKDGTGIEDILEAIVE
HHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHH
RIPAPDDKEDDHLKALIFDSYYDTYRGVITYLRVFSGQLVPGMEIKMMQTGKKFEASEIG
HCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCC
ILNPGPKKVDALNAGEVGFMAANIKDVKDSRVGDTITSYKKPAPEPLAGYKQVKPMVYCG
CCCCCCCCCCCCCCCCCEEEECCCHHHCCCCCCCHHHHCCCCCCCCCCCHHHCCCEEEEE
LFPADGEDYEDLRDALDKLQLNDASLVYEQENSAALGFGFRCGFLGLLHMEIIQERLERE
ECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
YGISLVTTAPNVVYRVELVEGEEITIENPTDLPERNEINAIYEPVVRANFMVPQDYIGPV
HCCEEEEECCCEEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHH
MELCEEKRGEYETLEYLDSLRVLLKYKLPLSEIVFDFFDLLKSKTRGYASFDYEIIGYEG
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECEEEEECCC
ADLVKVDILVNKEPVDALSFIVHQDKAYYKGRQLAGKLREMLPRQMFDVPVQAAIGNKVI
CCEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHH
ARETVKAVKKNVLAKCYGGDITRKRKLLEKQKEGKQRMKQVGKVDIPQEAFMAVLSIDK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA