Click here to switch to the map view.

The map label for this gene is hemL

Identifier: 188585757

GI number: 188585757

Start: 1198149

End: 1199441

Strand: Direct

Name: hemL

Synonym: Nther_1131

Alternate gene names: 188585757

Gene position: 1198149-1199441 (Clockwise)

Preceding gene: 188585756

Following gene: 188585758

Centisome position: 37.85

GC content: 39.44

Gene sequence:

>1293_bases
TTGCGTTATGCTAATTCGCAAAAGTATTTTAAAACAGCTTGTGATTTGATGCCCGGAGGAGTTAACAGTCCAGCCCGTGC
TTTTAGCTCGGTAGAGAGTGACCCATTATTTATTAAAGATGGATCAGGTTCTAAGGTTTACGATGTTGATGATAATGAAT
ACATTGACTATGTAGCGTCCTATGGGCCATTAATTTTAGGCCATTGTCATCCCCAAATAAGAGATGCTTTAAAAGAACAA
ATCGATTACGGTACTGGTTATGGATCACCTACAGAACTGGAAAATGAAATGGCAGAACTAGTGATTGAGGCAGTACCTTC
TATAGAAATGGTTAGAATGGTGAATTCCGGGACAGAAGCTACTATGAGTGCATTGAGACTTGCTCGCGGATATACCGGAA
AAAACAAAATAGTCAAACTGACTGGCTGTTATCACGGACATTCTGATAGTTTATTGATAAAAGCAGGATCGGGTGTGACA
ACTCTTGGATTGCCAGATAGTCCCGGGGTACCAGCCAGTATTGCCGAGAATACAATTACAGTACCTTACAATAACTTAGA
AGTTATGGAACAAGTGTTTAATAAATATGGAGATGATATTGCAGGTGTGATATTGGAACCTGTTGCTGGTAATATGGGTG
TGGTTCCGCCTAATGAAGGATATCTGGAAGGGTTGCGAGAACTTACTAGCAAATACGGTTCTTTACTAATTTTTGATGAA
GTGATGACTGGTTTTAGAGTTTCTTATGGTGGTGCACAAGCTTATTACGGAGTTACACCAGATCTAACTTGTCTTGGTAA
GGTTATTGGTGGCGGATTACCTGTTGGTGCTTATGGCGGCAAAGAGGAGATTATGGAGTACATAGCACCAGCTGGATCAA
TTTATCAAGCTGGAACTTTATCAGGTAATCCCATAGCTATGAGGGCAGGAATTGAAACCTTGAAAGTCTTAAGACAAGAG
GGTAGCTACGACCAATTAGAATCAAAAAGTAAACATCTAGCAGAAGGATTGGCAAAAGTGGCAGATAAAAATGGAGTATC
AGTGACTATTAACCGAGTAGGTACAATGGTAGGGATGTATTTCACAGAAGGACCAGTAACTGATTTTGAAAGTGCAACCA
GTTCAGATAACGATAAGTTTGTAAAATATTTTGAGACTATGTTATCTGAAGGTGTGTATTTGGCACCTAGTCAATTTGAA
GCTATGTTTATGTCTTTAGTACACACCCAGGAGGATATTGAAAAAACCATACAAATTGCCGATAAAGCTTTGTCGAAAGC
AGCTCAGGTATAA

Upstream 100 bases:

>100_bases
TATACAGTACAAAGGAGTATAAGAAAACTAGCATGACTTATTTTAATTAATTGTCAAATTTGAATTGTCAAATCTGATAA
GGAATAAGGAGGGATTGATT

Downstream 100 bases:

>100_bases
TTTGAAAAGTCGTCACGCACTCTCGTGACTTTAGTCATAAGAGGTACACAGTATAGTTATACACTGGAATTACTCTAAAT
AAGTAATATCAAACCGGCGA

Product: glutamate-1-semialdehyde 2,1-aminomutase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MRYANSQKYFKTACDLMPGGVNSPARAFSSVESDPLFIKDGSGSKVYDVDDNEYIDYVASYGPLILGHCHPQIRDALKEQ
IDYGTGYGSPTELENEMAELVIEAVPSIEMVRMVNSGTEATMSALRLARGYTGKNKIVKLTGCYHGHSDSLLIKAGSGVT
TLGLPDSPGVPASIAENTITVPYNNLEVMEQVFNKYGDDIAGVILEPVAGNMGVVPPNEGYLEGLRELTSKYGSLLIFDE
VMTGFRVSYGGAQAYYGVTPDLTCLGKVIGGGLPVGAYGGKEEIMEYIAPAGSIYQAGTLSGNPIAMRAGIETLKVLRQE
GSYDQLESKSKHLAEGLAKVADKNGVSVTINRVGTMVGMYFTEGPVTDFESATSSDNDKFVKYFETMLSEGVYLAPSQFE
AMFMSLVHTQEDIEKTIQIADKALSKAAQV

Sequences:

>Translated_430_residues
MRYANSQKYFKTACDLMPGGVNSPARAFSSVESDPLFIKDGSGSKVYDVDDNEYIDYVASYGPLILGHCHPQIRDALKEQ
IDYGTGYGSPTELENEMAELVIEAVPSIEMVRMVNSGTEATMSALRLARGYTGKNKIVKLTGCYHGHSDSLLIKAGSGVT
TLGLPDSPGVPASIAENTITVPYNNLEVMEQVFNKYGDDIAGVILEPVAGNMGVVPPNEGYLEGLRELTSKYGSLLIFDE
VMTGFRVSYGGAQAYYGVTPDLTCLGKVIGGGLPVGAYGGKEEIMEYIAPAGSIYQAGTLSGNPIAMRAGIETLKVLRQE
GSYDQLESKSKHLAEGLAKVADKNGVSVTINRVGTMVGMYFTEGPVTDFESATSSDNDKFVKYFETMLSEGVYLAPSQFE
AMFMSLVHTQEDIEKTIQIADKALSKAAQV
>Mature_430_residues
MRYANSQKYFKTACDLMPGGVNSPARAFSSVESDPLFIKDGSGSKVYDVDDNEYIDYVASYGPLILGHCHPQIRDALKEQ
IDYGTGYGSPTELENEMAELVIEAVPSIEMVRMVNSGTEATMSALRLARGYTGKNKIVKLTGCYHGHSDSLLIKAGSGVT
TLGLPDSPGVPASIAENTITVPYNNLEVMEQVFNKYGDDIAGVILEPVAGNMGVVPPNEGYLEGLRELTSKYGSLLIFDE
VMTGFRVSYGGAQAYYGVTPDLTCLGKVIGGGLPVGAYGGKEEIMEYIAPAGSIYQAGTLSGNPIAMRAGIETLKVLRQE
GSYDQLESKSKHLAEGLAKVADKNGVSVTINRVGTMVGMYFTEGPVTDFESATSSDNDKFVKYFETMLSEGVYLAPSQFE
AMFMSLVHTQEDIEKTIQIADKALSKAAQV

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=299, Percent_Identity=34.4481605351171, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI13994255, Length=369, Percent_Identity=24.9322493224932, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI24119277, Length=359, Percent_Identity=28.9693593314763, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI284507298, Length=324, Percent_Identity=28.7037037037037, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI37574042, Length=347, Percent_Identity=27.0893371757925, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI226442709, Length=314, Percent_Identity=27.7070063694268, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI226442705, Length=346, Percent_Identity=26.0115606936416, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=54.7169811320755, Blast_Score=496, Evalue=1e-142,
Organism=Escherichia coli, GI1788044, Length=333, Percent_Identity=31.8318318318318, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI145693181, Length=314, Percent_Identity=31.5286624203822, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789759, Length=329, Percent_Identity=30.3951367781155, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789016, Length=346, Percent_Identity=28.6127167630058, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787560, Length=379, Percent_Identity=26.3852242744063, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1786991, Length=380, Percent_Identity=25.5263157894737, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144271, Length=333, Percent_Identity=30.6306306306306, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71992977, Length=373, Percent_Identity=27.8820375335121, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32564660, Length=371, Percent_Identity=27.7628032345013, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=185, Percent_Identity=35.1351351351351, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323470, Length=342, Percent_Identity=29.2397660818713, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324432, Length=364, Percent_Identity=26.9230769230769, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357415, Length=299, Percent_Identity=31.1036789297659, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161085790, Length=444, Percent_Identity=27.2522522522523, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI28574759, Length=410, Percent_Identity=28.0487804878049, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_NATTJ (B2A1H4)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917302.1
- ProteinModelPortal:   B2A1H4
- SMR:   B2A1H4
- GeneID:   6315390
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1131
- HOGENOM:   HBG725944
- OMA:   SVKHTEE
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46203; Mature: 46203

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYANSQKYFKTACDLMPGGVNSPARAFSSVESDPLFIKDGSGSKVYDVDDNEYIDYVAS
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCCEEEECCCCHHHHHHHH
YGPLILGHCHPQIRDALKEQIDYGTGYGSPTELENEMAELVIEAVPSIEMVRMVNSGTEA
CCCEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHH
TMSALRLARGYTGKNKIVKLTGCYHGHSDSLLIKAGSGVTTLGLPDSPGVPASIAENTIT
HHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEECCCEEEECCCCCCCCCHHHCCCEEE
VPYNNLEVMEQVFNKYGDDIAGVILEPVAGNMGVVPPNEGYLEGLRELTSKYGSLLIFDE
ECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEHH
VMTGFRVSYGGAQAYYGVTPDLTCLGKVIGGGLPVGAYGGKEEIMEYIAPAGSIYQAGTL
HHHCCEEECCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPIAMRAGIETLKVLRQEGSYDQLESKSKHLAEGLAKVADKNGVSVTINRVGTMVGMY
CCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHH
FTEGPVTDFESATSSDNDKFVKYFETMLSEGVYLAPSQFEAMFMSLVHTQEDIEKTIQIA
CCCCCCCCCHHCCCCCCHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
DKALSKAAQV
HHHHHHHHCC
>Mature Secondary Structure
MRYANSQKYFKTACDLMPGGVNSPARAFSSVESDPLFIKDGSGSKVYDVDDNEYIDYVAS
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCCEEEECCCCHHHHHHHH
YGPLILGHCHPQIRDALKEQIDYGTGYGSPTELENEMAELVIEAVPSIEMVRMVNSGTEA
CCCEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHH
TMSALRLARGYTGKNKIVKLTGCYHGHSDSLLIKAGSGVTTLGLPDSPGVPASIAENTIT
HHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEECCCEEEECCCCCCCCCHHHCCCEEE
VPYNNLEVMEQVFNKYGDDIAGVILEPVAGNMGVVPPNEGYLEGLRELTSKYGSLLIFDE
ECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEHH
VMTGFRVSYGGAQAYYGVTPDLTCLGKVIGGGLPVGAYGGKEEIMEYIAPAGSIYQAGTL
HHHCCEEECCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPIAMRAGIETLKVLRQEGSYDQLESKSKHLAEGLAKVADKNGVSVTINRVGTMVGMY
CCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHH
FTEGPVTDFESATSSDNDKFVKYFETMLSEGVYLAPSQFEAMFMSLVHTQEDIEKTIQIA
CCCCCCCCCHHCCCCCCHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
DKALSKAAQV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA