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The map label for this gene is nitA [H]

Identifier: 188585699

GI number: 188585699

Start: 1129814

End: 1130743

Strand: Direct

Name: nitA [H]

Synonym: Nther_1072

Alternate gene names: 188585699

Gene position: 1129814-1130743 (Clockwise)

Preceding gene: 188585698

Following gene: 188585701

Centisome position: 35.69

GC content: 36.02

Gene sequence:

>930_bases
TTGAAAACCAATCAATATGTAAGAACAGCAGTAATTCAAGCAGCACCAATTATAATGGATAGAGAGAAAACTTTAAATAA
AGCTTTGGATTTAATCAAAGAAGCGAAAAATGAACAAGCTGAAATAGTGGTTTTTCCTGAAGCCTTTATACCCGCTTATC
CACGAGGGCTTTCCTTTGGATATTTAGTAGGAAGTCGGACAATGGAAGGAAGAAACGATTTTTTGAGATATTATAAAAAT
GCTGTCCCAGTTCCTGGTGATACTACTGAAAAATTGGGAGAAGCAGCAAGAAAAGCAGGAATTTATTTAGTGATTGGAGT
TACGGAAATTGATACTGAAAAAAATAATAACACTTTATATTGTACAATTTTGTACTTTGGCCCCGATGGAAGACTTTTAG
ATAAGCATCAAAAATTAAAACCTACTGGGTCTGAAAGACTTATATGGGGAGAAGGGGATGGTAGTACGCTATCAGTTGTA
AATACTCCCCATGGTAAATTAGGTGGTTTGATTTGCTGGGAAAATTACATGCCTCTTGCACGAACCGCTATGTATCAAAA
AGGTGTCAGTTTATATGTTGCTCCCACAGCAGATGCCAGATCAGTTTGGCAATCAACTATTAGGCATATAGCCTTAGAAG
GCAGATGTTATGTGCTGTCTTGTAATCAGTTTGTAACCAAGGATATGTATCCTGATGATCTTGAATATTATCATGAACTA
CAAAATCAGCCCAATATTATGTGTTCAGGAGGTAGTGCCATAGTCAATCCTATGGGTGAATATGTAGTGGAACCTGTGTA
TAATAAGGAAGAAATGTTGTTGGCAGACTTGAATATGGATCTAGTTATAAAAGGAAAGATGGACTTTGATGTAGTTGGTC
ACTATGACCGAACTGATGTATTTCAGCTGAAAATAAATGAAAATGAATAA

Upstream 100 bases:

>100_bases
CATAGGAGCTTTTTAAATCACTGGGCTAAGTTATAAACTTGTCATAAAATATATTTGGGGGATAATATTAAAGTTATGAT
TAACTTGGGAGGAATATAAA

Downstream 100 bases:

>100_bases
GAAGTAGTTATTCTATAAGTAAGGCCAATTCTAAGGTCAATTTAGTCAATTTAAGCTATTTGAGATCAATCAGTTTTACT
TGGATTTGAGATTTAAAGTA

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKTNQYVRTAVIQAAPIIMDREKTLNKALDLIKEAKNEQAEIVVFPEAFIPAYPRGLSFGYLVGSRTMEGRNDFLRYYKN
AVPVPGDTTEKLGEAARKAGIYLVIGVTEIDTEKNNNTLYCTILYFGPDGRLLDKHQKLKPTGSERLIWGEGDGSTLSVV
NTPHGKLGGLICWENYMPLARTAMYQKGVSLYVAPTADARSVWQSTIRHIALEGRCYVLSCNQFVTKDMYPDDLEYYHEL
QNQPNIMCSGGSAIVNPMGEYVVEPVYNKEEMLLADLNMDLVIKGKMDFDVVGHYDRTDVFQLKINENE

Sequences:

>Translated_309_residues
MKTNQYVRTAVIQAAPIIMDREKTLNKALDLIKEAKNEQAEIVVFPEAFIPAYPRGLSFGYLVGSRTMEGRNDFLRYYKN
AVPVPGDTTEKLGEAARKAGIYLVIGVTEIDTEKNNNTLYCTILYFGPDGRLLDKHQKLKPTGSERLIWGEGDGSTLSVV
NTPHGKLGGLICWENYMPLARTAMYQKGVSLYVAPTADARSVWQSTIRHIALEGRCYVLSCNQFVTKDMYPDDLEYYHEL
QNQPNIMCSGGSAIVNPMGEYVVEPVYNKEEMLLADLNMDLVIKGKMDFDVVGHYDRTDVFQLKINENE
>Mature_309_residues
MKTNQYVRTAVIQAAPIIMDREKTLNKALDLIKEAKNEQAEIVVFPEAFIPAYPRGLSFGYLVGSRTMEGRNDFLRYYKN
AVPVPGDTTEKLGEAARKAGIYLVIGVTEIDTEKNNNTLYCTILYFGPDGRLLDKHQKLKPTGSERLIWGEGDGSTLSVV
NTPHGKLGGLICWENYMPLARTAMYQKGVSLYVAPTADARSVWQSTIRHIALEGRCYVLSCNQFVTKDMYPDDLEYYHEL
QNQPNIMCSGGSAIVNPMGEYVVEPVYNKEEMLLADLNMDLVIKGKMDFDVVGHYDRTDVFQLKINENE

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=313, Percent_Identity=26.517571884984, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17557111, Length=301, Percent_Identity=45.8471760797342, Blast_Score=283, Evalue=7e-77,
Organism=Saccharomyces cerevisiae, GI6322027, Length=197, Percent_Identity=42.6395939086294, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6322026, Length=110, Percent_Identity=35.4545454545455, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323383, Length=298, Percent_Identity=25.1677852348993, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 34815; Mature: 34815

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNQYVRTAVIQAAPIIMDREKTLNKALDLIKEAKNEQAEIVVFPEAFIPAYPRGLSFG
CCCCHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCEEE
YLVGSRTMEGRNDFLRYYKNAVPVPGDTTEKLGEAARKAGIYLVIGVTEIDTEKNNNTLY
EEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCEEE
CTILYFGPDGRLLDKHQKLKPTGSERLIWGEGDGSTLSVVNTPHGKLGGLICWENYMPLA
EEEEEECCCCCEECCHHCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCHH
RTAMYQKGVSLYVAPTADARSVWQSTIRHIALEGRCYVLSCNQFVTKDMYPDDLEYYHEL
HHHHHHCCCEEEEECCCCHHHHHHHHHHHEEECCEEEEEECCHHHHCCCCCCHHHHHHHH
QNQPNIMCSGGSAIVNPMGEYVVEPVYNKEEMLLADLNMDLVIKGKMDFDVVGHYDRTDV
CCCCCEEECCCCHHHCCCHHHHCCCCCCCCEEEEEECCCCEEEECCCCEEEECCCCCCEE
FQLKINENE
EEEEECCCC
>Mature Secondary Structure
MKTNQYVRTAVIQAAPIIMDREKTLNKALDLIKEAKNEQAEIVVFPEAFIPAYPRGLSFG
CCCCHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCEEE
YLVGSRTMEGRNDFLRYYKNAVPVPGDTTEKLGEAARKAGIYLVIGVTEIDTEKNNNTLY
EEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCEEE
CTILYFGPDGRLLDKHQKLKPTGSERLIWGEGDGSTLSVVNTPHGKLGGLICWENYMPLA
EEEEEECCCCCEECCHHCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCHH
RTAMYQKGVSLYVAPTADARSVWQSTIRHIALEGRCYVLSCNQFVTKDMYPDDLEYYHEL
HHHHHHCCCEEEEECCCCHHHHHHHHHHHEEECCEEEEEECCHHHHCCCCCCHHHHHHHH
QNQPNIMCSGGSAIVNPMGEYVVEPVYNKEEMLLADLNMDLVIKGKMDFDVVGHYDRTDV
CCCCCEEECCCCHHHCCCHHHHCCCCCCCCEEEEEECCCCEEEECCCCEEEECCCCCCEE
FQLKINENE
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]