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The map label for this gene is nuoE [H]

Identifier: 188584738

GI number: 188584738

Start: 111915

End: 112403

Strand: Direct

Name: nuoE [H]

Synonym: Nther_0096

Alternate gene names: 188584738

Gene position: 111915-112403 (Clockwise)

Preceding gene: 188584737

Following gene: 188584739

Centisome position: 3.54

GC content: 40.08

Gene sequence:

>489_bases
ATGGCTGAATGTAAATGCGGTAGCGCTGCTAAGAAAAAAGAAGAACAACAGCTCGAAGAAATTTTAGCCCAATACAAAAA
CGAAAAAGGAGCTTTAATTACTGCTTTACAGAAAGTTCAAGAGTTTTATGGCTATTTGCCTGAAGAAGGTATGCAAAAAG
TAGCTGAAGCCTTTGACATGCCTGAAAGTAAAGTCTTTGGGGTAGCAACATTTTATGCCCAGTTCCATTTACAACCTCGA
GGTGAATGGGTGATCCGGGTTTGTACTGGAACGGCTTGTCATGTGCGAGGGGCAGAGAAAATTATGAATAAGTTGATTGA
AGAACTGAATATTGAACCTGGTGAAACTACAGAGGATTTAAAATTCACTCTCGAGCCTGTGGCATGTATCGGGTGTTGTG
GTCTGGCCCCCGTTATTATGGTGAATGATAACACTCATGGTCGATTAGTTCCAGATCAAATTCCAGAGATTCTAGACAAA
TATAAATAA

Upstream 100 bases:

>100_bases
TCAAGGATTCAGGGAGGTGATACTTAATTAAAGGGGGATACGGTAAAAATTTATTAAAATTAGATATTAGACTTTGATTT
TATAGAGAGGAGGAATAGAT

Downstream 100 bases:

>100_bases
TAATGAAGGAACTTTCATTACATCTGTTAGATTTAATACAAAATGCCATTGAGGCTTCTGCTACTAAAATTCAAGTTGAC
TTTTACTTGAGTTATCAAGA

Product: NADH-quinone oxidoreductase, E subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5 [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MAECKCGSAAKKKEEQQLEEILAQYKNEKGALITALQKVQEFYGYLPEEGMQKVAEAFDMPESKVFGVATFYAQFHLQPR
GEWVIRVCTGTACHVRGAEKIMNKLIEELNIEPGETTEDLKFTLEPVACIGCCGLAPVIMVNDNTHGRLVPDQIPEILDK
YK

Sequences:

>Translated_162_residues
MAECKCGSAAKKKEEQQLEEILAQYKNEKGALITALQKVQEFYGYLPEEGMQKVAEAFDMPESKVFGVATFYAQFHLQPR
GEWVIRVCTGTACHVRGAEKIMNKLIEELNIEPGETTEDLKFTLEPVACIGCCGLAPVIMVNDNTHGRLVPDQIPEILDK
YK
>Mature_161_residues
AECKCGSAAKKKEEQQLEEILAQYKNEKGALITALQKVQEFYGYLPEEGMQKVAEAFDMPESKVFGVATFYAQFHLQPRG
EWVIRVCTGTACHVRGAEKIMNKLIEELNIEPGETTEDLKFTLEPVACIGCCGLAPVIMVNDNTHGRLVPDQIPEILDKY
K

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=149, Percent_Identity=37.5838926174497, Blast_Score=107, Evalue=6e-24,
Organism=Escherichia coli, GI1788621, Length=149, Percent_Identity=40.9395973154362, Blast_Score=132, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17561328, Length=153, Percent_Identity=38.562091503268, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18859877, Length=151, Percent_Identity=38.4105960264901, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21355353, Length=149, Percent_Identity=36.9127516778523, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18126; Mature: 17995

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAECKCGSAAKKKEEQQLEEILAQYKNEKGALITALQKVQEFYGYLPEEGMQKVAEAFDM
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
PESKVFGVATFYAQFHLQPRGEWVIRVCTGTACHVRGAEKIMNKLIEELNIEPGETTEDL
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHH
KFTLEPVACIGCCGLAPVIMVNDNTHGRLVPDQIPEILDKYK
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
AECKCGSAAKKKEEQQLEEILAQYKNEKGALITALQKVQEFYGYLPEEGMQKVAEAFDM
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
PESKVFGVATFYAQFHLQPRGEWVIRVCTGTACHVRGAEKIMNKLIEELNIEPGETTEDL
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHH
KFTLEPVACIGCCGLAPVIMVNDNTHGRLVPDQIPEILDKYK
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]