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The map label for this gene is hup2 [H]

Identifier: 188584712

GI number: 188584712

Start: 86675

End: 86953

Strand: Direct

Name: hup2 [H]

Synonym: Nther_0070

Alternate gene names: 188584712

Gene position: 86675-86953 (Clockwise)

Preceding gene: 188584711

Following gene: 188584713

Centisome position: 2.74

GC content: 35.13

Gene sequence:

>279_bases
ATGAACAAAACTGAACTTATTAGTAAAGTTGCAGAAAGAAGTGAAATGACTAAAAAAGACACAGAAAAAGTTGTCAACGA
GGTTTTCGATGTAATTTCTGATCAGTTGGCAAAAGAAGACAAAGTGCAGTTGATTGGTTTTGGAACTTTTGAGGTGAGGG
AGAGAGCTGCTAGAGAAGGTCGAAATCCATCTACAGGTGAAGTAATTCAGATTGAAGCTACTAAAGTTCCAGCTTTTAAA
GCAGGAAAAGCTTTAAAAGAAAAAGTGAAAAATGGATAA

Upstream 100 bases:

>100_bases
TTTAGCAGGAATTACAAAAAAATCCTCGAATAGCTAGAAAAGGAGATTAAAGTTCAGTATTGGCGGTTCAAAAGACTAAT
TTTTAGGAGGGATTTACTTT

Downstream 100 bases:

>100_bases
GCAATCGTAAAGTAGAACATAATTTTAAGCGGGCCCATTGGCCCGCTTTTATTATATATATTGTATATCTCCCTACCAAG
TATCAGATACTAATAAAAAA

Product: nucleoid protein Hbs

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 92; Mature: 92

Protein sequence:

>92_residues
MNKTELISKVAERSEMTKKDTEKVVNEVFDVISDQLAKEDKVQLIGFGTFEVRERAAREGRNPSTGEVIQIEATKVPAFK
AGKALKEKVKNG

Sequences:

>Translated_92_residues
MNKTELISKVAERSEMTKKDTEKVVNEVFDVISDQLAKEDKVQLIGFGTFEVRERAAREGRNPSTGEVIQIEATKVPAFK
AGKALKEKVKNG
>Mature_92_residues
MNKTELISKVAERSEMTKKDTEKVVNEVFDVISDQLAKEDKVQLIGFGTFEVRERAAREGRNPSTGEVIQIEATKVPAFK
AGKALKEKVKNG

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=48.314606741573, Blast_Score=94, Evalue=2e-21,
Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=50, Blast_Score=94, Evalue=2e-21,
Organism=Escherichia coli, GI1787141, Length=90, Percent_Identity=37.7777777777778, Blast_Score=75, Evalue=8e-16,
Organism=Escherichia coli, GI1788005, Length=91, Percent_Identity=36.2637362637363, Blast_Score=66, Evalue=3e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 10237; Mature: 10237

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTELISKVAERSEMTKKDTEKVVNEVFDVISDQLAKEDKVQLIGFGTFEVRERAAREG
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCC
RNPSTGEVIQIEATKVPAFKAGKALKEKVKNG
CCCCCCCEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKTELISKVAERSEMTKKDTEKVVNEVFDVISDQLAKEDKVQLIGFGTFEVRERAAREG
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCC
RNPSTGEVIQIEATKVPAFKAGKALKEKVKNG
CCCCCCCEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]