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The map label for this gene is gatB

Identifier: 188527493

GI number: 188527493

Start: 683458

End: 684882

Strand: Reverse

Name: gatB

Synonym: HPSH_03565

Alternate gene names: 188527493

Gene position: 684882-683458 (Counterclockwise)

Preceding gene: 188527494

Following gene: 188527492

Centisome position: 42.58

GC content: 41.26

Gene sequence:

>1425_bases
ATGCCATTTGAAGCTGTAATCGGGCTAGAAGTCCATGTCCAACTCAACACCAAAACCAAAATCTTTTGCTCTTGCTCTAC
AAGCTTTGGAGAATCCCCTAATTCTAACACTTGCCCTGTGTGTTTGGGCTTACCGGGAGCTTTGCCGGTATTGAATAAAG
AAGTGGTTAAAAAAGCCATCCAATTAGGCACAGCCATTGAAGCCAACATCAACCAATATTCCATTTTTGCAAGGAAAAAT
TATTTTTACCCTGATTTGCCTAAGGCTTATCAAATTTCGCAGTTTGAAGTCCCTATTGTGAGCGATGGGAAATTAGAGAT
TGACACTAAAGAGGGCGCAAAAATCGTGCGTATTGAAAGAGCCCACATGGAAGAAGACGCTGGTAAAAATATCCATGAGG
GCAGCTGTTCTTTAGTGGATTTGAACCGCGCTTGCACCCCTTTATTAGAAATTGTCAGTAAGCCGGACATGCGAAATAGT
GAAGAAGCCATAGCGTATTTGAAAAAGCTCCATGCTATCGTGCGTTTTATAGGGATTTCTGATGCGAACATGCAAGAGGG
GAATTTCAGGTGCGATGCGAATGTATCCATTAGACCCAAAGGCGATGAAAAGCTTTACACGAGGGTGGAGATTAAAAACT
TAAATAGCTTTAGATTCATCGCTAAAGCGATTGAATACGAAATAGAGCGCCAAAGCACGGCGTGGGAAAGCGGGCGTTAT
CATGAAGAAGTGGTTCAAGAAACGCGCCTTTTTGACACCCATAAAGGGATCACCCTTTCTATGCGCAATAAAGAAGAATC
AGCGGATTACCGCTATTTTAAAGATCCGGATTTGTATCCTGTTTTTATTGATGAAAAATTTTTAAAAGAAGCTCAAAAGA
TCAATGAATTGCCTAGCGCGAAAAAAATCCGCTACATGAAAGATTTTAATATTAAAGAAGACGATGCGAATTTATTGGTG
AGCGATCCTTTATTGGCAGAATATTTTGAAAGCATGCTCAATCTTGGGGTTAAGGCTAAAACGAGTGTAACATGGCTTTG
CGTGGAATTGTTAGGGCGCTTGAAAGCCGAAATCACTTTAGAAAATTGCGGAGTTAGCGCTCACATGTTAGGCGTTTTAG
CCAAACGCATTGATGAGGGCAAGATTTCTGGCAAGAGCGCTAAAGATGTGTTAGACAGGCTTTTAGAAGAACAAGGGGGC
GATGTGGATGCGCTCATTGAACAAATGGGCTTATCTCAAGTCAATGACACAGAAGCGATTGTTAAAGTGATAGAAGAGGT
GCTTAAAAACAACGCTGATAAGGTGCTTGAATACAAAAGCGGTAAGGACAAGCTTTTTGGGTTTTTTGTGGGGCAGGCGA
TGAAAAATTTAAAAGGCGCTAATCCTAGCGTGGTGAATGCTATTTTGAAAGAGAAATTGGATTGA

Upstream 100 bases:

>100_bases
AAAAAATGAGTTTGAAAGAGATCAATGATTTCATTAAAGCCCATACCGAAATCAACGACTTGACTTTTGCCATTGTGAGC
AACAAAAAGAAGGACAAATG

Downstream 100 bases:

>100_bases
TGAGGAAAATTTTTTCTTATGTTTTGAAGGCTTTGTTGTTTTTTGGGATCGCTCATGCAGAGCCTGAATCTAAAGTAGAA
GCCTTAGAAGGGAAGAAACA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MPFEAVIGLEVHVQLNTKTKIFCSCSTSFGESPNSNTCPVCLGLPGALPVLNKEVVKKAIQLGTAIEANINQYSIFARKN
YFYPDLPKAYQISQFEVPIVSDGKLEIDTKEGAKIVRIERAHMEEDAGKNIHEGSCSLVDLNRACTPLLEIVSKPDMRNS
EEAIAYLKKLHAIVRFIGISDANMQEGNFRCDANVSIRPKGDEKLYTRVEIKNLNSFRFIAKAIEYEIERQSTAWESGRY
HEEVVQETRLFDTHKGITLSMRNKEESADYRYFKDPDLYPVFIDEKFLKEAQKINELPSAKKIRYMKDFNIKEDDANLLV
SDPLLAEYFESMLNLGVKAKTSVTWLCVELLGRLKAEITLENCGVSAHMLGVLAKRIDEGKISGKSAKDVLDRLLEEQGG
DVDALIEQMGLSQVNDTEAIVKVIEEVLKNNADKVLEYKSGKDKLFGFFVGQAMKNLKGANPSVVNAILKEKLD

Sequences:

>Translated_474_residues
MPFEAVIGLEVHVQLNTKTKIFCSCSTSFGESPNSNTCPVCLGLPGALPVLNKEVVKKAIQLGTAIEANINQYSIFARKN
YFYPDLPKAYQISQFEVPIVSDGKLEIDTKEGAKIVRIERAHMEEDAGKNIHEGSCSLVDLNRACTPLLEIVSKPDMRNS
EEAIAYLKKLHAIVRFIGISDANMQEGNFRCDANVSIRPKGDEKLYTRVEIKNLNSFRFIAKAIEYEIERQSTAWESGRY
HEEVVQETRLFDTHKGITLSMRNKEESADYRYFKDPDLYPVFIDEKFLKEAQKINELPSAKKIRYMKDFNIKEDDANLLV
SDPLLAEYFESMLNLGVKAKTSVTWLCVELLGRLKAEITLENCGVSAHMLGVLAKRIDEGKISGKSAKDVLDRLLEEQGG
DVDALIEQMGLSQVNDTEAIVKVIEEVLKNNADKVLEYKSGKDKLFGFFVGQAMKNLKGANPSVVNAILKEKLD
>Mature_473_residues
PFEAVIGLEVHVQLNTKTKIFCSCSTSFGESPNSNTCPVCLGLPGALPVLNKEVVKKAIQLGTAIEANINQYSIFARKNY
FYPDLPKAYQISQFEVPIVSDGKLEIDTKEGAKIVRIERAHMEEDAGKNIHEGSCSLVDLNRACTPLLEIVSKPDMRNSE
EAIAYLKKLHAIVRFIGISDANMQEGNFRCDANVSIRPKGDEKLYTRVEIKNLNSFRFIAKAIEYEIERQSTAWESGRYH
EEVVQETRLFDTHKGITLSMRNKEESADYRYFKDPDLYPVFIDEKFLKEAQKINELPSAKKIRYMKDFNIKEDDANLLVS
DPLLAEYFESMLNLGVKAKTSVTWLCVELLGRLKAEITLENCGVSAHMLGVLAKRIDEGKISGKSAKDVLDRLLEEQGGD
VDALIEQMGLSQVNDTEAIVKVIEEVLKNNADKVLEYKSGKDKLFGFFVGQAMKNLKGANPSVVNAILKEKLD

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=34.5381526104418, Blast_Score=271, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI115534094, Length=306, Percent_Identity=39.8692810457516, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319391, Length=499, Percent_Identity=30.8617234468938, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45550808, Length=474, Percent_Identity=33.3333333333333, Blast_Score=249, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53220; Mature: 53089

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFEAVIGLEVHVQLNTKTKIFCSCSTSFGESPNSNTCPVCLGLPGALPVLNKEVVKKAI
CCCCEEEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
QLGTAIEANINQYSIFARKNYFYPDLPKAYQISQFEVPIVSDGKLEIDTKEGAKIVRIER
HHCCCEECCCCEEEEEEECCCCCCCCCCCEECCEEECEEECCCEEEEECCCCCEEEEEEH
AHMEEDAGKNIHEGSCSLVDLNRACTPLLEIVSKPDMRNSEEAIAYLKKLHAIVRFIGIS
HHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DANMQEGNFRCDANVSIRPKGDEKLYTRVEIKNLNSFRFIAKAIEYEIERQSTAWESGRY
CCCCCCCCEEECCCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCH
HEEVVQETRLFDTHKGITLSMRNKEESADYRYFKDPDLYPVFIDEKFLKEAQKINELPSA
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCH
KKIRYMKDFNIKEDDANLLVSDPLLAEYFESMLNLGVKAKTSVTWLCVELLGRLKAEITL
HHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEE
ENCGVSAHMLGVLAKRIDEGKISGKSAKDVLDRLLEEQGGDVDALIEQMGLSQVNDTEAI
ECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHH
VKVIEEVLKNNADKVLEYKSGKDKLFGFFVGQAMKNLKGANPSVVNAILKEKLD
HHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PFEAVIGLEVHVQLNTKTKIFCSCSTSFGESPNSNTCPVCLGLPGALPVLNKEVVKKAI
CCCEEEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
QLGTAIEANINQYSIFARKNYFYPDLPKAYQISQFEVPIVSDGKLEIDTKEGAKIVRIER
HHCCCEECCCCEEEEEEECCCCCCCCCCCEECCEEECEEECCCEEEEECCCCCEEEEEEH
AHMEEDAGKNIHEGSCSLVDLNRACTPLLEIVSKPDMRNSEEAIAYLKKLHAIVRFIGIS
HHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DANMQEGNFRCDANVSIRPKGDEKLYTRVEIKNLNSFRFIAKAIEYEIERQSTAWESGRY
CCCCCCCCEEECCCEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCH
HEEVVQETRLFDTHKGITLSMRNKEESADYRYFKDPDLYPVFIDEKFLKEAQKINELPSA
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCH
KKIRYMKDFNIKEDDANLLVSDPLLAEYFESMLNLGVKAKTSVTWLCVELLGRLKAEITL
HHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEE
ENCGVSAHMLGVLAKRIDEGKISGKSAKDVLDRLLEEQGGDVDALIEQMGLSQVNDTEAI
ECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHH
VKVIEEVLKNNADKVLEYKSGKDKLFGFFVGQAMKNLKGANPSVVNAILKEKLD
HHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA