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The map label for this gene is glcF [H]

Identifier: 188527485

GI number: 188527485

Start: 675703

End: 676992

Strand: Reverse

Name: glcF [H]

Synonym: HPSH_03525

Alternate gene names: 188527485

Gene position: 676992-675703 (Counterclockwise)

Preceding gene: 188527486

Following gene: 188527484

Centisome position: 42.09

GC content: 39.15

Gene sequence:

>1290_bases
ATGAATGAAAATATTTTTGAAGAAGTAGGGGACGCTTGCGTTAAATGCGCTAAATGCGTGCCAGGTTGCACCATATACCG
CATCCATAAAGACGAAACGACTTCGCCTAGAGGCTTTTTAGATTTGATGCGCCTAAACGCTCAAAACAAGCTCAAATTAG
ACACGAATTTAAAACACCTTTTAGAAACTTGTTTTTTATGCACCGCTTGCGTGGAGATTTGCCCCTTTCATTTGCCTATA
GACACTTTAATAGAAAAAGCCAGAGAAAAAATCGCTCAAAAATACGGCATTGCTTGGTATAAAAAATCCTATTTTTCCCT
TTTAAAAAACCGCAAAAAAATGGATAAGGTGTTTTCGGTGGCGCATTTTTTAGCCCCTTGTATCTTCAAGCAAGTAGGGG
ATAGTTTAGAGCCTAGAATGGCGTTTAAAGGCTTATTCAAACGCTTTAAAAAAAGCACACTGCCTCCCTTAAACCAAAAA
AGTTTTTTGCAAAAGCATGCAGAAATAAAGCCTTTAGAAAACCCCATTCAAAAAGTGGCGGTTTTTATAGGGTGTTTAAG
CAATTACCATTACCAGCAAGTGGGGGAAAGCTTGTTGTATATTTTAGAAAAACTCAACATTCAAGCGATCATCCCTAAGC
AAGAATGCTGCTCAGCGCCGGCGTATTTTACCGGCGATAAAGACACCACGCTTTTTTTAGTGAAAAAAAACATAGAATGG
TTTGAAAGCTATTTAGATGAAGTGGATGCGATCATTGTGCCTGAAGCCACATGCGCTAGCATGCTCATCAACGATTATTA
CAAGGTGTTTTTAGGCGAAAAAGATAAGGATTTGTATGTGAAGCGCTTGGAAAAAATCACGCCTAAAATCTATCTGGCGA
GCGTGTTTTTAGAGAGACACACCCCTTTAAAACATCTTTTAGAAAAAATCCCTAAGGGAAAAAAAGAGACTATCACCTAT
CATAACCCTTGCCATGCCAAAAAAACCCTAAACGCTCATAAAGAAGTGCGCAACTTGCTCAATTCGCATTATGAAATTAA
AGAAATGCCGGATCATTGTTGCGGTTTTGGGGGGATTACGATGCAAACAGAAAAGGCGGGATTTTCTTTAAAAGTAGGTC
TTCTTAGGGCTAAGGAAATCATAGACACTAAAGCTGAAATTTTAAGCGCTGAATGCGGGGCATGCCACATGCAATTAAAC
AACGCCTTAAAGTCTTTAGACGACCCTAACACCCCGCAATTTTCACACCCTTTAGAACTCATCGCTAAAGCTTTAAAAAG
CGCTGAATAA

Upstream 100 bases:

>100_bases
GCTTTGAGATGACCAAAACAGGGGGCATGCTCGTAAGAAACATGGCTATGGAGTTTGACGCGTATTTGCGTGGGGGCGAA
AAACATTTCAGTAAAACGCT

Downstream 100 bases:

>100_bases
AAAGCCTTTTTAACCCCACTTTCTAACATCTTTTTATATAATACAGAACTTTAACGCCACTATTTAAGGAATGAAAAATG
TCAGAAATCAGCACCTACAA

Product: anaerobic glycerol-3-phosphate dehydrogenase, subunit C (glpC)

Products: FADH2; dihydroxy-acetone-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNENIFEEVGDACVKCAKCVPGCTIYRIHKDETTSPRGFLDLMRLNAQNKLKLDTNLKHLLETCFLCTACVEICPFHLPI
DTLIEKAREKIAQKYGIAWYKKSYFSLLKNRKKMDKVFSVAHFLAPCIFKQVGDSLEPRMAFKGLFKRFKKSTLPPLNQK
SFLQKHAEIKPLENPIQKVAVFIGCLSNYHYQQVGESLLYILEKLNIQAIIPKQECCSAPAYFTGDKDTTLFLVKKNIEW
FESYLDEVDAIIVPEATCASMLINDYYKVFLGEKDKDLYVKRLEKITPKIYLASVFLERHTPLKHLLEKIPKGKKETITY
HNPCHAKKTLNAHKEVRNLLNSHYEIKEMPDHCCGFGGITMQTEKAGFSLKVGLLRAKEIIDTKAEILSAECGACHMQLN
NALKSLDDPNTPQFSHPLELIAKALKSAE

Sequences:

>Translated_429_residues
MNENIFEEVGDACVKCAKCVPGCTIYRIHKDETTSPRGFLDLMRLNAQNKLKLDTNLKHLLETCFLCTACVEICPFHLPI
DTLIEKAREKIAQKYGIAWYKKSYFSLLKNRKKMDKVFSVAHFLAPCIFKQVGDSLEPRMAFKGLFKRFKKSTLPPLNQK
SFLQKHAEIKPLENPIQKVAVFIGCLSNYHYQQVGESLLYILEKLNIQAIIPKQECCSAPAYFTGDKDTTLFLVKKNIEW
FESYLDEVDAIIVPEATCASMLINDYYKVFLGEKDKDLYVKRLEKITPKIYLASVFLERHTPLKHLLEKIPKGKKETITY
HNPCHAKKTLNAHKEVRNLLNSHYEIKEMPDHCCGFGGITMQTEKAGFSLKVGLLRAKEIIDTKAEILSAECGACHMQLN
NALKSLDDPNTPQFSHPLELIAKALKSAE
>Mature_429_residues
MNENIFEEVGDACVKCAKCVPGCTIYRIHKDETTSPRGFLDLMRLNAQNKLKLDTNLKHLLETCFLCTACVEICPFHLPI
DTLIEKAREKIAQKYGIAWYKKSYFSLLKNRKKMDKVFSVAHFLAPCIFKQVGDSLEPRMAFKGLFKRFKKSTLPPLNQK
SFLQKHAEIKPLENPIQKVAVFIGCLSNYHYQQVGESLLYILEKLNIQAIIPKQECCSAPAYFTGDKDTTLFLVKKNIEW
FESYLDEVDAIIVPEATCASMLINDYYKVFLGEKDKDLYVKRLEKITPKIYLASVFLERHTPLKHLLEKIPKGKKETITY
HNPCHAKKTLNAHKEVRNLLNSHYEIKEMPDHCCGFGGITMQTEKAGFSLKVGLLRAKEIIDTKAEILSAECGACHMQLN
NALKSLDDPNTPQFSHPLELIAKALKSAE

Specific function: Electron Transfer Protein; May Also Function As The Membrane Anchor For The Glpab Dimer. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Loosely Bound To The Cytoplasm Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788576, Length=438, Percent_Identity=24.2009132420091, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI48994913, Length=360, Percent_Identity=24.1666666666667, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 48941; Mature: 48941

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENIFEEVGDACVKCAKCVPGCTIYRIHKDETTSPRGFLDLMRLNAQNKLKLDTNLKHL
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEECCCHHHH
LETCFLCTACVEICPFHLPIDTLIEKAREKIAQKYGIAWYKKSYFSLLKNRKKMDKVFSV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
AHFLAPCIFKQVGDSLEPRMAFKGLFKRFKKSTLPPLNQKSFLQKHAEIKPLENPIQKVA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHH
VFIGCLSNYHYQQVGESLLYILEKLNIQAIIPKQECCSAPAYFTGDKDTTLFLVKKNIEW
HHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCHHHHCCCCCEEECCCCCEEEEEECCHHH
FESYLDEVDAIIVPEATCASMLINDYYKVFLGEKDKDLYVKRLEKITPKIYLASVFLERH
HHHHHHHHCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHC
TPLKHLLEKIPKGKKETITYHNPCHAKKTLNAHKEVRNLLNSHYEIKEMPDHCCGFGGIT
CCHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHCCCHHHCCCCCCE
MQTEKAGFSLKVGLLRAKEIIDTKAEILSAECGACHMQLNNALKSLDDPNTPQFSHPLEL
EEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHH
IAKALKSAE
HHHHHHCCC
>Mature Secondary Structure
MNENIFEEVGDACVKCAKCVPGCTIYRIHKDETTSPRGFLDLMRLNAQNKLKLDTNLKHL
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEECCCHHHH
LETCFLCTACVEICPFHLPIDTLIEKAREKIAQKYGIAWYKKSYFSLLKNRKKMDKVFSV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
AHFLAPCIFKQVGDSLEPRMAFKGLFKRFKKSTLPPLNQKSFLQKHAEIKPLENPIQKVA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHH
VFIGCLSNYHYQQVGESLLYILEKLNIQAIIPKQECCSAPAYFTGDKDTTLFLVKKNIEW
HHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCHHHHCCCCCEEECCCCCEEEEEECCHHH
FESYLDEVDAIIVPEATCASMLINDYYKVFLGEKDKDLYVKRLEKITPKIYLASVFLERH
HHHHHHHHCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHC
TPLKHLLEKIPKGKKETITYHNPCHAKKTLNAHKEVRNLLNSHYEIKEMPDHCCGFGGIT
CCHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHCCCHHHCCCCCCE
MQTEKAGFSLKVGLLRAKEIIDTKAEILSAECGACHMQLNNALKSLDDPNTPQFSHPLEL
EEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHH
IAKALKSAE
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: FAD; glycerol-3-phosphate [C]

Specific reaction: FAD + glycerol-3-phosphate = FADH2 + dihydroxy-acetone-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]