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The map label for this gene is ruvC

Identifier: 188527278

GI number: 188527278

Start: 467410

End: 467883

Strand: Direct

Name: ruvC

Synonym: HPSH_02425

Alternate gene names: 188527278

Gene position: 467410-467883 (Clockwise)

Preceding gene: 188527276

Following gene: 188527280

Centisome position: 29.06

GC content: 43.25

Gene sequence:

>474_bases
ATGCGTATTTTAGGAATAGATCCGGGCAGTAGGAAATGCGGGTATGCTATCGTTTCTCACACTTCTAACAAGCTTTCTTT
AATCACGGCCGGATTCATTAACATCACCACGACACGCTTGCAAGAACAGATTTTAGACTTGATAGAAGCCTTAGATTGCT
TATTGGATCGTTACGAAGTTCATGAAGTGGCGATTGAAGATATTTTCTTTGGGTATAACCCTAAAAGCGTGATCAAGCTT
GCGCAATTCAGGGGGGCGTTGTCCTTAAAGATTTTAGAAAGGATCGGTAACTTTAGCGAATACACGCCCTTACAAGTCAA
AAAAGCCCTAACCGGTAACGGGAAAGCCGCTAAAGAGCAAGTAGCCTTTATGGTCAAACGCTTGCTTAACATCACAAGCG
AAATCAAGCCTTTGGATATTAGCGATGCGATAGCCGTTGCTATCACGCATGCGCAACGCTTAAAACCCCACTAA

Upstream 100 bases:

>100_bases
TAAAAGTTCTTAATGCAATCTTGGTATTTTGCGCTAACAAGAAACATGGATATAATAATAGCGTTTTTTAAACCTTGCGA
TAAACAAACAGGAATGGTTG

Downstream 100 bases:

>100_bases
TCACCATTTGTAAGCCACTTCAAAGCGCGCGTTAAACCCGGGCTCTGCGATCGCTCTTTTAATAGCGTCAGTGCCTGGCA
TGTCCGGGCTCATGACATAA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRILGIDPGSRKCGYAIVSHTSNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEVHEVAIEDIFFGYNPKSVIKL
AQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQVAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKPH

Sequences:

>Translated_157_residues
MRILGIDPGSRKCGYAIVSHTSNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEVHEVAIEDIFFGYNPKSVIKL
AQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQVAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKPH
>Mature_157_residues
MRILGIDPGSRKCGYAIVSHTSNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEVHEVAIEDIFFGYNPKSVIKL
AQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQVAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKPH

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=131, Percent_Identity=32.0610687022901, Blast_Score=74, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 17448; Mature: 17448

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILGIDPGSRKCGYAIVSHTSNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEV
CEEECCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEVAIEDIFFGYNPKSVIKLAQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQ
HHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHH
VAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKPH
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRILGIDPGSRKCGYAIVSHTSNKLSLITAGFINITTTRLQEQILDLIEALDCLLDRYEV
CEEECCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEVAIEDIFFGYNPKSVIKLAQFRGALSLKILERIGNFSEYTPLQVKKALTGNGKAAKEQ
HHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHH
VAFMVKRLLNITSEIKPLDISDAIAVAITHAQRLKPH
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA