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The map label for this gene is pyrB

Identifier: 188527171

GI number: 188527171

Start: 362121

End: 363044

Strand: Direct

Name: pyrB

Synonym: HPSH_01890

Alternate gene names: 188527171

Gene position: 362121-363044 (Clockwise)

Preceding gene: 188527162

Following gene: 188527173

Centisome position: 22.51

GC content: 41.34

Gene sequence:

>924_bases
ATGCCAAAAAAATGCCGACACTTGCTCCAAACCAGCGATTTAAGCCTAGATGAAATCAAGCTTTTATTAAAAAAAGCGAG
CGTTTATGCGAACGATTTTAACGCCGTATCTTTAGAAACAAAAGAAAAAATGCAAAATAAAATCATCGTGGCTTTATTTT
TTGAAAATTCCACCAGAACGGTGTCTAGTTTTGAAATCGCAAGCTTGAGATTGGGGGCAAAAATCGTGAAATTAAACATG
CAAACAAGCTCCGCTTCAAAGGGTGAAACCCTAACAGACACTTTTAAAAATATCTATGCCATGCAGCCTGACGCTATCAT
CACACGGCATGCTTTTTCAAGCGCGCCTTTTAAATTAGCCGAATTTTCACAATGCCCCTTGATTAACGCAGGAAGCGGCG
TAAGCGCTCATCCTACCCAAGCGTTATTAGATTTGCTCACCCTTTATCAGCATTTTGGCAGTTTAGAAAATTTAAAAGGG
AAGAAAATCGCTTTCATAGGCGATGTGAAAAATTCCAGAGTGGCTAATAGCAACATTAAATTGCTCCAAAGGCTAGGGCT
TGAGATCATGCTGTGCGCTCCAAGCTCCATGCTCCCTAGCGTTTCTTTAAAAACGACGCATAACATTGAAGAAGCGATAG
AGTTTGCAGACATTTTAATGAGTTTGAGGACTCAAACCGAACGGCACAACGCGCCCATTTTTGCGAGCTTGAAAGATTAT
GGAAACGCTTATTGCATCACCCAAAAACGCCTAGAGGCTCACGCTAAAAATAAAGAGATCATTATTTTACACCCAGGCCC
GGTGCATAGGGATATTGATATAGAAAGCGCGGTGTTAGAAGATAAGCGATCTAAAGTTTTAGAGCAGGTCAAAAACGGCG
TGGCGATGCGCATGGCGGTGTTGGAGTTTTTGCTATTAGATTGA

Upstream 100 bases:

>100_bases
ACACCAGAAGCGTTGAGAGATTTTTCAAGCCCATTCCTTGCTAAAACCCATGCCATTTTTAGCTAAAATAAAGTCTATAT
TATAACTAAAAGGGGGTTTT

Downstream 100 bases:

>100_bases
TTGTCAAACTTAAAAAATGCTTTTTGGCATTTTTAAAATCAATGTGAGAGCTTTCATATTAAAAAAATGGGTTTGGTTAT
AAAACAGCACAAAAATCCCA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPKKCRHLLQTSDLSLDEIKLLLKKASVYANDFNAVSLETKEKMQNKIIVALFFENSTRTVSSFEIASLRLGAKIVKLNM
QTSSASKGETLTDTFKNIYAMQPDAIITRHAFSSAPFKLAEFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKG
KKIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPSVSLKTTHNIEEAIEFADILMSLRTQTERHNAPIFASLKDY
GNAYCITQKRLEAHAKNKEIIILHPGPVHRDIDIESAVLEDKRSKVLEQVKNGVAMRMAVLEFLLLD

Sequences:

>Translated_307_residues
MPKKCRHLLQTSDLSLDEIKLLLKKASVYANDFNAVSLETKEKMQNKIIVALFFENSTRTVSSFEIASLRLGAKIVKLNM
QTSSASKGETLTDTFKNIYAMQPDAIITRHAFSSAPFKLAEFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKG
KKIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPSVSLKTTHNIEEAIEFADILMSLRTQTERHNAPIFASLKDY
GNAYCITQKRLEAHAKNKEIIILHPGPVHRDIDIESAVLEDKRSKVLEQVKNGVAMRMAVLEFLLLD
>Mature_306_residues
PKKCRHLLQTSDLSLDEIKLLLKKASVYANDFNAVSLETKEKMQNKIIVALFFENSTRTVSSFEIASLRLGAKIVKLNMQ
TSSASKGETLTDTFKNIYAMQPDAIITRHAFSSAPFKLAEFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKGK
KIAFIGDVKNSRVANSNIKLLQRLGLEIMLCAPSSMLPSVSLKTTHNIEEAIEFADILMSLRTQTERHNAPIFASLKDYG
NAYCITQKRLEAHAKNKEIIILHPGPVHRDIDIESAVLEDKRSKVLEQVKNGVAMRMAVLEFLLLD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=278, Percent_Identity=34.1726618705036, Blast_Score=141, Evalue=9e-34,
Organism=Escherichia coli, GI2367364, Length=320, Percent_Identity=32.5, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1790703, Length=330, Percent_Identity=26.0606060606061, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994908, Length=250, Percent_Identity=30, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786469, Length=204, Percent_Identity=29.9019607843137, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=288, Percent_Identity=32.9861111111111, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=318, Percent_Identity=35.5345911949685, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322373, Length=341, Percent_Identity=24.3401759530792, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24642586, Length=283, Percent_Identity=33.2155477031802, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34140; Mature: 34009

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKCRHLLQTSDLSLDEIKLLLKKASVYANDFNAVSLETKEKMQNKIIVALFFENSTRT
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCEEEEEEECCCCCE
VSSFEIASLRLGAKIVKLNMQTSSASKGETLTDTFKNIYAMQPDAIITRHAFSSAPFKLA
EHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHH
EFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKGKKIAFIGDVKNSRVANSNIK
HHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEECCCCCCCCCCHHH
LLQRLGLEIMLCAPSSMLPSVSLKTTHNIEEAIEFADILMSLRTQTERHNAPIFASLKDY
HHHHCCCEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHC
GNAYCITQKRLEAHAKNKEIIILHPGPVHRDIDIESAVLEDKRSKVLEQVKNGVAMRMAV
CCEEEEEHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LEFLLLD
HHHHHCC
>Mature Secondary Structure 
PKKCRHLLQTSDLSLDEIKLLLKKASVYANDFNAVSLETKEKMQNKIIVALFFENSTRT
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHCCCEEEEEEECCCCCE
VSSFEIASLRLGAKIVKLNMQTSSASKGETLTDTFKNIYAMQPDAIITRHAFSSAPFKLA
EHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHH
EFSQCPLINAGSGVSAHPTQALLDLLTLYQHFGSLENLKGKKIAFIGDVKNSRVANSNIK
HHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEEEECCCCCCCCCCHHH
LLQRLGLEIMLCAPSSMLPSVSLKTTHNIEEAIEFADILMSLRTQTERHNAPIFASLKDY
HHHHCCCEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHC
GNAYCITQKRLEAHAKNKEIIILHPGPVHRDIDIESAVLEDKRSKVLEQVKNGVAMRMAV
CCEEEEEHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
LEFLLLD
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA