Click here to switch to the map view.

The map label for this gene is pyrB [H]

Identifier: 187935477

GI number: 187935477

Start: 2691201

End: 2692124

Strand: Reverse

Name: pyrB [H]

Synonym: CLL_A2581

Alternate gene names: 187935477

Gene position: 2692124-2691201 (Counterclockwise)

Preceding gene: 187934313

Following gene: 187932380

Centisome position: 70.84

GC content: 29.98

Gene sequence:

>924_bases
ATGATAAAAGATAAACATTTAATAGACCCAATGGATTTTACAGTAGAAGAATTAGAAGAAATTTTTAAATTAGCACATGA
AATAATTTTAGATCCAGAGAAATTTTCTCATGTCTGTGAAGGGAAAATATTAGGAACTTTATTTTACGAGCCAAGTACAA
GAACTAGATTTAGTTTTGAAGCAGCTATGATGAGACTTGGTGGAAAAATACTTGGCTTTTCTGAGCCAAATTCATCATCA
GCTTCAAAAGGTGAAAGCTTATCAGATACAATCAAAATGGTGTCAATTTATACTGATATAATAGCGATGAGACATCCTAA
AGAGGGATCAGCTAAAGTAGCAAGTCTTTATTCAAGTGTTCCAATCATTAATGCAGGAGATGGTGGACATCAACATCCAA
CTCAAACTCTTACTGATTTATTAACAATAGAAATGCTTAAGGACGGTTTAAGTAACCATACTATCGGAATATGTGGTGAC
TTAAAGTATGGAAGAACAGTTCATTCGTTAATAAAAGCAATGTCTAGATACAAAGGTAATAAATTTTTACTTATCTCTCC
AAAAGAATTAAGAATACCAGATTATATAAGAGAAGAAATACTTAGAAAAAACAATATTGAGTTTTTAGAAGTAGAAACAT
TAGAAGAAGTTATAGATAAAGTAGATATATTATATATGACTAGAATTCAAAAAGAAAGATTCTTCAATGAAGAAGAATAC
TTAAGACTTAGAGATAGTTATATATTAAATAAGGAAAAAATGAATTTAGCAAAAAGCGATATGATAGTTATGCATCCTCT
GCCAAGAGTAAATGAAATTGCATGTGAAGTTGATTACGATAAAAGAGCAGCTTACTTTAAACAAGCTGAATATGGAATGT
ATGCAAGAATGGCATTAATGGCTAAACTTTTGGGGGTGTTATAA

Upstream 100 bases:

>100_bases
TTAAATCTATGAAATATGGCACTATCTAAAAGACTTTTAGATCGCTTTGGTATAACTTTTTATAAGTTTTTCTGTATACT
TAAAAAGGAGTGATATCAAT

Downstream 100 bases:

>100_bases
GATGTTAGAAATCACAAGTTTAAAAAATGGATTAGTTATAGATCATATTGAAGCAGGAATGGGAATAAAAATATTTAACT
ATCTAGGTTTAGATAACAGT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIKDKHLIDPMDFTVEELEEIFKLAHEIILDPEKFSHVCEGKILGTLFYEPSTRTRFSFEAAMMRLGGKILGFSEPNSSS
ASKGESLSDTIKMVSIYTDIIAMRHPKEGSAKVASLYSSVPIINAGDGGHQHPTQTLTDLLTIEMLKDGLSNHTIGICGD
LKYGRTVHSLIKAMSRYKGNKFLLISPKELRIPDYIREEILRKNNIEFLEVETLEEVIDKVDILYMTRIQKERFFNEEEY
LRLRDSYILNKEKMNLAKSDMIVMHPLPRVNEIACEVDYDKRAAYFKQAEYGMYARMALMAKLLGVL

Sequences:

>Translated_307_residues
MIKDKHLIDPMDFTVEELEEIFKLAHEIILDPEKFSHVCEGKILGTLFYEPSTRTRFSFEAAMMRLGGKILGFSEPNSSS
ASKGESLSDTIKMVSIYTDIIAMRHPKEGSAKVASLYSSVPIINAGDGGHQHPTQTLTDLLTIEMLKDGLSNHTIGICGD
LKYGRTVHSLIKAMSRYKGNKFLLISPKELRIPDYIREEILRKNNIEFLEVETLEEVIDKVDILYMTRIQKERFFNEEEY
LRLRDSYILNKEKMNLAKSDMIVMHPLPRVNEIACEVDYDKRAAYFKQAEYGMYARMALMAKLLGVL
>Mature_307_residues
MIKDKHLIDPMDFTVEELEEIFKLAHEIILDPEKFSHVCEGKILGTLFYEPSTRTRFSFEAAMMRLGGKILGFSEPNSSS
ASKGESLSDTIKMVSIYTDIIAMRHPKEGSAKVASLYSSVPIINAGDGGHQHPTQTLTDLLTIEMLKDGLSNHTIGICGD
LKYGRTVHSLIKAMSRYKGNKFLLISPKELRIPDYIREEILRKNNIEFLEVETLEEVIDKVDILYMTRIQKERFFNEEEY
LRLRDSYILNKEKMNLAKSDMIVMHPLPRVNEIACEVDYDKRAAYFKQAEYGMYARMALMAKLLGVL

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=303, Percent_Identity=44.5544554455446, Blast_Score=274, Evalue=7e-74,
Organism=Homo sapiens, GI38788445, Length=315, Percent_Identity=27.3015873015873, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI2367364, Length=303, Percent_Identity=44.8844884488449, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1790703, Length=277, Percent_Identity=25.9927797833935, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=24.251497005988, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=307, Percent_Identity=42.3452768729642, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=44.7712418300654, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6322373, Length=270, Percent_Identity=26.6666666666667, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24642586, Length=304, Percent_Identity=45.3947368421053, Blast_Score=254, Evalue=7e-68,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35157; Mature: 35157

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDKHLIDPMDFTVEELEEIFKLAHEIILDPEKFSHVCEGKILGTLFYEPSTRTRFSFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCCCHHHHH
AAMMRLGGKILGFSEPNSSSASKGESLSDTIKMVSIYTDIIAMRHPKEGSAKVASLYSSV
HHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
PIINAGDGGHQHPTQTLTDLLTIEMLKDGLSNHTIGICGDLKYGRTVHSLIKAMSRYKGN
CEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
KFLLISPKELRIPDYIREEILRKNNIEFLEVETLEEVIDKVDILYMTRIQKERFFNEEEY
EEEEECCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LRLRDSYILNKEKMNLAKSDMIVMHPLPRVNEIACEVDYDKRAAYFKQAEYGMYARMALM
HHHHHHHHCCHHHHHHHHCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
AKLLGVL
HHHHHCC
>Mature Secondary Structure
MIKDKHLIDPMDFTVEELEEIFKLAHEIILDPEKFSHVCEGKILGTLFYEPSTRTRFSFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEECCCCCCHHHHH
AAMMRLGGKILGFSEPNSSSASKGESLSDTIKMVSIYTDIIAMRHPKEGSAKVASLYSSV
HHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
PIINAGDGGHQHPTQTLTDLLTIEMLKDGLSNHTIGICGDLKYGRTVHSLIKAMSRYKGN
CEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
KFLLISPKELRIPDYIREEILRKNNIEFLEVETLEEVIDKVDILYMTRIQKERFFNEEEY
EEEEECCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LRLRDSYILNKEKMNLAKSDMIVMHPLPRVNEIACEVDYDKRAAYFKQAEYGMYARMALM
HHHHHHHHCCHHHHHHHHCCEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
AKLLGVL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA