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The map label for this gene is rfbA [H]

Identifier: 187935321

GI number: 187935321

Start: 3407802

End: 3408680

Strand: Reverse

Name: rfbA [H]

Synonym: CLL_A3253

Alternate gene names: 187935321

Gene position: 3408680-3407802 (Counterclockwise)

Preceding gene: 187934651

Following gene: 187935603

Centisome position: 89.69

GC content: 30.49

Gene sequence:

>879_bases
ATGAAGGGCATAATACTTGCAGGTGGAAGTGGGACAAGACTTTATCCACTAACAATGGTTACATCAAAACAACTTTTACC
GGTATATGACAAACCGATGATTTATTATCCACTATCAACTTTAATGTTAGCAGGAATTAAAGATATATTAATAATTTCAA
CGCCTAATGATTTACCTAATTTTCAAAAGTTATTAGGAGATGGATCAAGATATGGAATGAATTTATCTTATAAAGTTCAG
CCATCTCCAGATGGTTTAGCTCAAGCTTTTACATTAGGAGAAGAGTTTATAGGAAATGATAGTTGTGCAATGATACTTGG
AGATAATATATTCCATGGAAATGGTTTAACAGCACATTTAAAAAGAGCAGTTGAAAATGAAGGACGTGCAACAGTATTTG
GATATTATGTAGATGATCCAGAACGTTTTGGAGTAGTAGAATTTGATGATAAAGGAAAAGCAATATCATTAGAAGAAAAG
CCAGAAGTTCCAAAATCTAATTATGCTGTTACAGGACTTTATTTTTATGATAATAAAGTATGTGAATATGCTAAGAACTT
AAAGCCATCAGCTAGAGGTGAATTAGAAATTACAGATTTAAATAGAATATATCTTGAACAAGGTAAACTTGATGTAATAA
CACTTGGAAGAGGTTATGGATGGCTTGATACAGGAACAGTTGATAGTTTAACTGAAGCAACTGAATATGTAAAAGTTATT
GAAACTAGACAAGGTCTTAAGATTGCATGTTTAGAAGAAATTTCATATAAAAATGGTTGGATAGATAAAGAAACTTTACT
TGAAAGTGCTAAACAATATGGTAAAAGTCCATATGGGAAGCACTTATATAATGTAGCAAATAATAAAGTTATTTATTAA

Upstream 100 bases:

>100_bases
CGTAAATAAATGTAAACTACATTTATTTATTTGGCCCTTTATGTTAAAATAATAAGAAGAATGTTTAAAATTAAAAAAAT
AGAAAGAAATGAGGATTTTT

Downstream 100 bases:

>100_bases
ATGACAATAATCAATTGTCTATTGCAAGAGAGGTGCGTATAACAATGAATTTAATTAAAACTAAATTAGATGGTGTTTAT
ATAGTAGAACCACAAGTTTT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKGIILAGGSGTRLYPLTMVTSKQLLPVYDKPMIYYPLSTLMLAGIKDILIISTPNDLPNFQKLLGDGSRYGMNLSYKVQ
PSPDGLAQAFTLGEEFIGNDSCAMILGDNIFHGNGLTAHLKRAVENEGRATVFGYYVDDPERFGVVEFDDKGKAISLEEK
PEVPKSNYAVTGLYFYDNKVCEYAKNLKPSARGELEITDLNRIYLEQGKLDVITLGRGYGWLDTGTVDSLTEATEYVKVI
ETRQGLKIACLEEISYKNGWIDKETLLESAKQYGKSPYGKHLYNVANNKVIY

Sequences:

>Translated_292_residues
MKGIILAGGSGTRLYPLTMVTSKQLLPVYDKPMIYYPLSTLMLAGIKDILIISTPNDLPNFQKLLGDGSRYGMNLSYKVQ
PSPDGLAQAFTLGEEFIGNDSCAMILGDNIFHGNGLTAHLKRAVENEGRATVFGYYVDDPERFGVVEFDDKGKAISLEEK
PEVPKSNYAVTGLYFYDNKVCEYAKNLKPSARGELEITDLNRIYLEQGKLDVITLGRGYGWLDTGTVDSLTEATEYVKVI
ETRQGLKIACLEEISYKNGWIDKETLLESAKQYGKSPYGKHLYNVANNKVIY
>Mature_292_residues
MKGIILAGGSGTRLYPLTMVTSKQLLPVYDKPMIYYPLSTLMLAGIKDILIISTPNDLPNFQKLLGDGSRYGMNLSYKVQ
PSPDGLAQAFTLGEEFIGNDSCAMILGDNIFHGNGLTAHLKRAVENEGRATVFGYYVDDPERFGVVEFDDKGKAISLEEK
PEVPKSNYAVTGLYFYDNKVCEYAKNLKPSARGELEITDLNRIYLEQGKLDVITLGRGYGWLDTGTVDSLTEATEYVKVI
ETRQGLKIACLEEISYKNGWIDKETLLESAKQYGKSPYGKHLYNVANNKVIY

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761619, Length=247, Percent_Identity=24.2914979757085, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI11761621, Length=247, Percent_Identity=24.2914979757085, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788351, Length=287, Percent_Identity=68.9895470383275, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1790224, Length=289, Percent_Identity=62.6297577854671, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI133931050, Length=196, Percent_Identity=29.5918367346939, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320148, Length=278, Percent_Identity=27.3381294964029, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355443, Length=251, Percent_Identity=24.3027888446215, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24644084, Length=251, Percent_Identity=24.3027888446215, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32490; Mature: 32490

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLYPLTMVTSKQLLPVYDKPMIYYPLSTLMLAGIKDILIISTPNDLPN
CCCEEEECCCCCEEEEEEEECCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCHH
FQKLLGDGSRYGMNLSYKVQPSPDGLAQAFTLGEEFIGNDSCAMILGDNIFHGNGLTAHL
HHHHHCCCCHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEECCCHHHHH
KRAVENEGRATVFGYYVDDPERFGVVEFDDKGKAISLEEKPEVPKSNYAVTGLYFYDNKV
HHHHHCCCCEEEEEEEECCHHHCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCHH
CEYAKNLKPSARGELEITDLNRIYLEQGKLDVITLGRGYGWLDTGTVDSLTEATEYVKVI
HHHHHCCCCCCCCCEEEEECCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
ETRQGLKIACLEEISYKNGWIDKETLLESAKQYGKSPYGKHLYNVANNKVIY
HHCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEEC
>Mature Secondary Structure
MKGIILAGGSGTRLYPLTMVTSKQLLPVYDKPMIYYPLSTLMLAGIKDILIISTPNDLPN
CCCEEEECCCCCEEEEEEEECCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCHH
FQKLLGDGSRYGMNLSYKVQPSPDGLAQAFTLGEEFIGNDSCAMILGDNIFHGNGLTAHL
HHHHHCCCCHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEECCCHHHHH
KRAVENEGRATVFGYYVDDPERFGVVEFDDKGKAISLEEKPEVPKSNYAVTGLYFYDNKV
HHHHHCCCCEEEEEEEECCHHHCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCHH
CEYAKNLKPSARGELEITDLNRIYLEQGKLDVITLGRGYGWLDTGTVDSLTEATEYVKVI
HHHHHCCCCCCCCCEEEEECCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
ETRQGLKIACLEEISYKNGWIDKETLLESAKQYGKSPYGKHLYNVANNKVIY
HHCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]