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The map label for this gene is rfbB [H]

Identifier: 187934855

GI number: 187934855

Start: 3406082

End: 3407101

Strand: Reverse

Name: rfbB [H]

Synonym: CLL_A3251

Alternate gene names: 187934855

Gene position: 3407101-3406082 (Counterclockwise)

Preceding gene: 187935603

Following gene: 187933644

Centisome position: 89.65

GC content: 28.82

Gene sequence:

>1020_bases
ATGAAAATAGTAGTAACTGGTGGAGCAGGATTTATTGGTGGAAATTTTGTTCATTATATGCTTAATAAATATAATGATTA
TAAAATAATTTGTGTTGATGCTTTAACTTATGCTGGAAATATGGAAACATTAGAAAGTGTTAAAGATAGTAAAAACTTTA
GTTTTCATAAAATAGATATAGCAGATAGAAAAGCTGTATATGATATGTTTAAAAAAGAAAATCCAGATATAGTAGTTAAT
TTTGCAGCAGAGAGTCATGTGGATAGATCAATAGAAAATCCAGAAATATTTTTAAAAACTAATATTATGGGTACTGCTGT
TTTAATGGATGCTTGTATAAAATACGGAATAAAAAGATATCATCAAGTATCAACAGATGAAGTATATGGGGATTTACCAA
TTGACAGACCAGATTTATTCTTTACAGAAGAAACACCAATACATACATCAAGCCCATATTCTTCAAGTAAGGCATCAGCA
GATCTTTTAGTTGGAGCATATAACAGAACTTATGGATTACAAACTACAATTTCAAGATGTTCAAATAACTATGGACCATA
CCATTTTCCAGAAAAGTTGATTCCATTAATGATAGCAAATGCATTAAATGATAAAGAATTACCTGTATACGGAAGTGGAG
AAAATATCAGAGATTGGCTTTATGTTGAAGATCACTGTAGAGCTATTGATATTATTATTCATAACGGAACAATAGGAGAA
GTTTATAATATTGGTGGACATAATGAAAGAAGTAATTTAGATGTAGTTAAAACAATTATTCATGAATTAGGAAAGTCTGA
AGAGTTAATTAAATTTGTAGGTGATAGAAAAGGTCATGATATGAGATATGCAATAGATCCTAGCAAGATTCATAGTGAAC
TTGGATGGCTTCCAACTACAAGTTTTGATGAAGGAATTAAAAAGACTATAAAATGGTATTTAGATAATAGAACTTGGTGG
GAAAATATATTAAGTGGTGAATATTCAAACTACTATGAGAATATGTATGCAAACAGATAA

Upstream 100 bases:

>100_bases
TAAGTAATATCTATGCATATTACTAAGATTAAGTTCAGACAAATAGAATTTGTTAAATTTATTATAGAGTTTTAGATAAA
TGGTTAGAGGAGATAAATAT

Downstream 100 bases:

>100_bases
GTGCTTAGTATCTATATTATTAGCTGTCTATAAGCCAAATGAAAACTGGCTTATAGAACAGCTCATTTCATTAAATAGTC
AAACTTATGGTAATTTAGAA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKIVVTGGAGFIGGNFVHYMLNKYNDYKIICVDALTYAGNMETLESVKDSKNFSFHKIDIADRKAVYDMFKKENPDIVVN
FAAESHVDRSIENPEIFLKTNIMGTAVLMDACIKYGIKRYHQVSTDEVYGDLPIDRPDLFFTEETPIHTSSPYSSSKASA
DLLVGAYNRTYGLQTTISRCSNNYGPYHFPEKLIPLMIANALNDKELPVYGSGENIRDWLYVEDHCRAIDIIIHNGTIGE
VYNIGGHNERSNLDVVKTIIHELGKSEELIKFVGDRKGHDMRYAIDPSKIHSELGWLPTTSFDEGIKKTIKWYLDNRTWW
ENILSGEYSNYYENMYANR

Sequences:

>Translated_339_residues
MKIVVTGGAGFIGGNFVHYMLNKYNDYKIICVDALTYAGNMETLESVKDSKNFSFHKIDIADRKAVYDMFKKENPDIVVN
FAAESHVDRSIENPEIFLKTNIMGTAVLMDACIKYGIKRYHQVSTDEVYGDLPIDRPDLFFTEETPIHTSSPYSSSKASA
DLLVGAYNRTYGLQTTISRCSNNYGPYHFPEKLIPLMIANALNDKELPVYGSGENIRDWLYVEDHCRAIDIIIHNGTIGE
VYNIGGHNERSNLDVVKTIIHELGKSEELIKFVGDRKGHDMRYAIDPSKIHSELGWLPTTSFDEGIKKTIKWYLDNRTWW
ENILSGEYSNYYENMYANR
>Mature_339_residues
MKIVVTGGAGFIGGNFVHYMLNKYNDYKIICVDALTYAGNMETLESVKDSKNFSFHKIDIADRKAVYDMFKKENPDIVVN
FAAESHVDRSIENPEIFLKTNIMGTAVLMDACIKYGIKRYHQVSTDEVYGDLPIDRPDLFFTEETPIHTSSPYSSSKASA
DLLVGAYNRTYGLQTTISRCSNNYGPYHFPEKLIPLMIANALNDKELPVYGSGENIRDWLYVEDHCRAIDIIIHNGTIGE
VYNIGGHNERSNLDVVKTIIHELGKSEELIKFVGDRKGHDMRYAIDPSKIHSELGWLPTTSFDEGIKKTIKWYLDNRTWW
ENILSGEYSNYYENMYANR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=328, Percent_Identity=37.5, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI42516563, Length=315, Percent_Identity=25.3968253968254, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56237023, Length=349, Percent_Identity=27.2206303724928, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI56118217, Length=349, Percent_Identity=27.2206303724928, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI189083684, Length=349, Percent_Identity=27.2206303724928, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4504031, Length=337, Percent_Identity=24.6290801186944, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=54.5977011494253, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1788353, Length=363, Percent_Identity=52.0661157024793, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1786974, Length=337, Percent_Identity=25.8160237388724, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788366, Length=350, Percent_Identity=24.8571428571429, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788365, Length=310, Percent_Identity=24.5161290322581, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=33.8509316770186, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI115532424, Length=320, Percent_Identity=32.1875, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI71982035, Length=356, Percent_Identity=27.8089887640449, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=358, Percent_Identity=27.6536312849162, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17539532, Length=317, Percent_Identity=25.5520504731861, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507723, Length=340, Percent_Identity=22.9411764705882, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901786, Length=338, Percent_Identity=23.3727810650888, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=332, Percent_Identity=23.4939759036145, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=340, Percent_Identity=22.6470588235294, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=330, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=330, Percent_Identity=24.2424242424242, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=343, Percent_Identity=25.9475218658892, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19923002, Length=354, Percent_Identity=28.8135593220339, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21356223, Length=315, Percent_Identity=25.7142857142857, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24158427, Length=340, Percent_Identity=23.5294117647059, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38722; Mature: 38722

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVTGGAGFIGGNFVHYMLNKYNDYKIICVDALTYAGNMETLESVKDSKNFSFHKIDI
CEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEEEHHCCCHHHHHHHHHCCCCCEEEEEC
ADRKAVYDMFKKENPDIVVNFAAESHVDRSIENPEIFLKTNIMGTAVLMDACIKYGIKRY
HHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
HQVSTDEVYGDLPIDRPDLFFTEETPIHTSSPYSSSKASADLLVGAYNRTYGLQTTISRC
HCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
SNNYGPYHFPEKLIPLMIANALNDKELPVYGSGENIRDWLYVEDHCRAIDIIIHNGTIGE
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCEEEEEEECCCCCE
VYNIGGHNERSNLDVVKTIIHELGKSEELIKFVGDRKGHDMRYAIDPSKIHSELGWLPTT
EEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCCC
SFDEGIKKTIKWYLDNRTWWENILSGEYSNYYENMYANR
CHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKIVVTGGAGFIGGNFVHYMLNKYNDYKIICVDALTYAGNMETLESVKDSKNFSFHKIDI
CEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEEEHHCCCHHHHHHHHHCCCCCEEEEEC
ADRKAVYDMFKKENPDIVVNFAAESHVDRSIENPEIFLKTNIMGTAVLMDACIKYGIKRY
HHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
HQVSTDEVYGDLPIDRPDLFFTEETPIHTSSPYSSSKASADLLVGAYNRTYGLQTTISRC
HCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
SNNYGPYHFPEKLIPLMIANALNDKELPVYGSGENIRDWLYVEDHCRAIDIIIHNGTIGE
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCEEEEEEECCCCCE
VYNIGGHNERSNLDVVKTIIHELGKSEELIKFVGDRKGHDMRYAIDPSKIHSELGWLPTT
EEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCCCCC
SFDEGIKKTIKWYLDNRTWWENILSGEYSNYYENMYANR
CHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]