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The map label for this gene is adh1 [H]

Identifier: 187934513

GI number: 187934513

Start: 3349486

End: 3350652

Strand: Reverse

Name: adh1 [H]

Synonym: CLL_A3215

Alternate gene names: 187934513

Gene position: 3350652-3349486 (Counterclockwise)

Preceding gene: 187935526

Following gene: 187933886

Centisome position: 88.17

GC content: 33.5

Gene sequence:

>1167_bases
ATGGCACGTTTTACATTACCAAGAGATTTATATCATGGTGAAGGATCTTTAGAAACACTTAAAACATTAAAAGGAAAGAA
AGCATTCGTAGTAGTTGGTGGTGGATCAATGAAAAGATTTGGCTTCCTTCAAAAAGTTGAAGATTATTTAAAAGAAGCAG
GAATGGAAGTACAATTATTTGAAGGTGTTGAACCAGACCCATCAGTAGAAACAGTAATGAAGGGTGCAGAAGCTATGAGA
AACTTCGAACCTGATTGGATAGTTGCAATGGGTGGAGGATCACCAATAGATGCTGCAAAAGCTATGTGGATATTCTATGA
ATACCCAGATTTTACTTTTGAACAAGCTGTTGTTCCATTTGGATTACCAGATTTAAGACAAAAAGCTAAATTTGTAGCTA
TTCCATCAACAAGTGGTACTGCTACAGAAGTTACAGCATTCTCAGTAATAACAGATTACAAAGCAAAAATTAAATATCCT
TTAGCTGACTTTAATATAACTCCAGATATAGCTATAGTTGATCCAGAATTAGCTCAAACAATGCCAGCTAAATTAGTAGC
TCATACTGGAATGGATGCATTAACTCATGCTATAGAAGCATATACTGCATCTCTAAGATCAAACTTCTCAGATCCATTAG
CTATTAAAGCAATAGAAATGGTAAACGAACATTTAGTTAAATCATTTGAAGGAGACAAAGAAGCTAGAAACTTAATGCAT
GAAGCACAATGTTTAGCAGGAATGGCTTTCTCAAATGCATTACTAGGAATAGTTCACTCTATGGCTCATAAAGTAGGAGC
TGTATTCCATATCCCTCATGGATGTGCTAATGCAATATTCTTACCATATGTAATTCAATATAACAGAATCGAATGTGAAG
ATAGATATGGTGATATTGCTAGAGCATTAAAATTAGAAGGAAATACTGATGCTGAATTAACTGATTCATTAATTAAATTA
ATCAATGATTTCAATGATGCATTAAATATTCCTCATTCAATGAAAGAATATGGTGTTGAAGAAGCAGACTTTAAGGCTAA
CGTTGAATTCATAGCTCATAATGCAGTATTAGATGCATGTACAGGATCAAGTCCTAGAGCAATAGATGACAAGACAATGG
AAAAATTACTTGGATGTACATTCTATGGAACTAAAGTTAATTTCTAA

Upstream 100 bases:

>100_bases
ATATTTATGTAAGTTGTTTAATTTTAAAATAACAAAAAACAATATATAAAATAATAAAAGCAATTAAGAAAATAAAGATA
TATTTTAGGAGGAACAGATT

Downstream 100 bases:

>100_bases
TACTTAGAAGTTAATAAGAAATTTATATATTAAAAAATTTACTAGGTAATTTAGTAGCACAAATTTGTTTATAGTTGATT
TTCAAATTTAATATATAGCA

Product: NADPH-dependent butanol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MARFTLPRDLYHGEGSLETLKTLKGKKAFVVVGGGSMKRFGFLQKVEDYLKEAGMEVQLFEGVEPDPSVETVMKGAEAMR
NFEPDWIVAMGGGSPIDAAKAMWIFYEYPDFTFEQAVVPFGLPDLRQKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYP
LADFNITPDIAIVDPELAQTMPAKLVAHTGMDALTHAIEAYTASLRSNFSDPLAIKAIEMVNEHLVKSFEGDKEARNLMH
EAQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCANAIFLPYVIQYNRIECEDRYGDIARALKLEGNTDAELTDSLIKL
INDFNDALNIPHSMKEYGVEEADFKANVEFIAHNAVLDACTGSSPRAIDDKTMEKLLGCTFYGTKVNF

Sequences:

>Translated_388_residues
MARFTLPRDLYHGEGSLETLKTLKGKKAFVVVGGGSMKRFGFLQKVEDYLKEAGMEVQLFEGVEPDPSVETVMKGAEAMR
NFEPDWIVAMGGGSPIDAAKAMWIFYEYPDFTFEQAVVPFGLPDLRQKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYP
LADFNITPDIAIVDPELAQTMPAKLVAHTGMDALTHAIEAYTASLRSNFSDPLAIKAIEMVNEHLVKSFEGDKEARNLMH
EAQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCANAIFLPYVIQYNRIECEDRYGDIARALKLEGNTDAELTDSLIKL
INDFNDALNIPHSMKEYGVEEADFKANVEFIAHNAVLDACTGSSPRAIDDKTMEKLLGCTFYGTKVNF
>Mature_387_residues
ARFTLPRDLYHGEGSLETLKTLKGKKAFVVVGGGSMKRFGFLQKVEDYLKEAGMEVQLFEGVEPDPSVETVMKGAEAMRN
FEPDWIVAMGGGSPIDAAKAMWIFYEYPDFTFEQAVVPFGLPDLRQKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYPL
ADFNITPDIAIVDPELAQTMPAKLVAHTGMDALTHAIEAYTASLRSNFSDPLAIKAIEMVNEHLVKSFEGDKEARNLMHE
AQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCANAIFLPYVIQYNRIECEDRYGDIARALKLEGNTDAELTDSLIKLI
NDFNDALNIPHSMKEYGVEEADFKANVEFIAHNAVLDACTGSSPRAIDDKTMEKLLGCTFYGTKVNF

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=368, Percent_Identity=26.9021739130435, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1787493, Length=414, Percent_Identity=41.7874396135266, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI1789163, Length=380, Percent_Identity=35.7894736842105, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI87082107, Length=384, Percent_Identity=34.8958333333333, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI48994951, Length=341, Percent_Identity=35.4838709677419, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789386, Length=357, Percent_Identity=25.7703081232493, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17537053, Length=401, Percent_Identity=25.6857855361596, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321181, Length=375, Percent_Identity=35.7333333333333, Blast_Score=222, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24657991, Length=369, Percent_Identity=26.5582655826558, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 42670; Mature: 42539

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFTLPRDLYHGEGSLETLKTLKGKKAFVVVGGGSMKRFGFLQKVEDYLKEAGMEVQLF
CCCCCCCHHHHCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
EGVEPDPSVETVMKGAEAMRNFEPDWIVAMGGGSPIDAAKAMWIFYEYPDFTFEQAVVPF
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHCCCCC
GLPDLRQKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYPLADFNITPDIAIVDPELAQT
CCHHHHHCCEEEEECCCCCCCHHHEEHHHHHCCHHEEECCEECCCCCCCEEEECHHHHHH
MPAKLVAHTGMDALTHAIEAYTASLRSNFSDPLAIKAIEMVNEHLVKSFEGDKEARNLMH
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EAQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCANAIFLPYVIQYNRIECEDRYGDIA
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHEECCCEECHHHHHHHH
RALKLEGNTDAELTDSLIKLINDFNDALNIPHSMKEYGVEEADFKANVEFIAHNAVLDAC
HHEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEHHHHHHHC
TGSSPRAIDDKTMEKLLGCTFYGTKVNF
CCCCCCCCCHHHHHHHHCCEEECCEECC
>Mature Secondary Structure 
ARFTLPRDLYHGEGSLETLKTLKGKKAFVVVGGGSMKRFGFLQKVEDYLKEAGMEVQLF
CCCCCCHHHHCCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
EGVEPDPSVETVMKGAEAMRNFEPDWIVAMGGGSPIDAAKAMWIFYEYPDFTFEQAVVPF
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHCCCCC
GLPDLRQKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYPLADFNITPDIAIVDPELAQT
CCHHHHHCCEEEEECCCCCCCHHHEEHHHHHCCHHEEECCEECCCCCCCEEEECHHHHHH
MPAKLVAHTGMDALTHAIEAYTASLRSNFSDPLAIKAIEMVNEHLVKSFEGDKEARNLMH
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EAQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCANAIFLPYVIQYNRIECEDRYGDIA
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHEECCCEECHHHHHHHH
RALKLEGNTDAELTDSLIKLINDFNDALNIPHSMKEYGVEEADFKANVEFIAHNAVLDAC
HHEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEHHHHHHHC
TGSSPRAIDDKTMEKLLGCTFYGTKVNF
CCCCCCCCCHHHHHHHHCCEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]