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The map label for this gene is asnB [H]

Identifier: 187934430

GI number: 187934430

Start: 2650024

End: 2651868

Strand: Reverse

Name: asnB [H]

Synonym: CLL_A2544

Alternate gene names: 187934430

Gene position: 2651868-2650024 (Counterclockwise)

Preceding gene: 187934558

Following gene: 187932439

Centisome position: 69.78

GC content: 28.08

Gene sequence:

>1845_bases
ATGTGTGGTATTGCTGGATTAGTAAATTTTAAAGAAGACATATCAAAATATAAAAATATATTAGAGGATATGGTAAAAAC
ATTAAAAAAAAGAGGCCCAGATAGTCAAGGATATTATGTTGATAAAAATGTTCTGTTAGGTCATAGAAGATTAATAGTTG
TAGATCCGGATGGTGGTAAACAGCCGATGATTAAGATATTTGAAGGCAATAAATATGTTTTAATTTATAATGGGGAGTTA
TATAATACGGAAGAATTAAGAAAAGAATTAAAGAACGAAGGATTTTATTTTGATTCATATTCAGATACAGAAGTATTAAT
TACATCATATATACGTTGGGGGAAAAATTGTATTAATAAATTAAATGGAATATTTGCTTTTGCTATTTTTGATGAAAAAA
ATAATGAAGTTTTTTTAGCTAGAGACCAAATGGGAGTAAAACCATTATTTTATACAGTATATAATGAAACTTTAATATTT
GGATCAGAAATAAAAACAATTTTAGCAAATTCAAATATAAAAAGAGAAATAAATGAAGATGGATTAACAGAATTATTTGC
ATTAGGACCAGCTGTAACTCCAGGAAAAGCTATATTTAAGGATATAAAAGAGATAGCACCTGCTAATTGCTTATTGATTT
CAAAAACTGGAGATATAAAAACATGGGAATATTGGAGCGTTGAGGCAAAAGAGTTTAAAGAAACATCAGCTGAAGCTATA
GATCATACTAGATATTTGTTAGTGGATGCAATAAAAAGACAATTGGTAGGGGATGTACCAATATGCACATTTCTTTCTGG
AGGGCTAGATTCTTCTGCAATTTCTGCAATAGCAGCACAAGAATATAGTAAGAAAGGAAAAACATTAACTACGTATTCTA
TAGATTATGAAGATAATTCTAAATATTTTAAGTCATCTTTATTTCAACCGACTACTGATGAATATTGGGCAGAGATTATG
GCAAAAGACATAGGAAGCGATCATAAAAAAATTACATTAAACCATGAAGATCTAGCAAATGCTTTAAAAGATGCAGTTAT
AGCTAGAGATTTACCAGGAATGGCAGATATAGATTCATCATTATTATTGTTTTGTAAAGAAATTCGTAAAGATTTTGTGG
TAGGTCTTTCAGGGGAATGTGCAGATGAAATATTTGCAGGGTATCCATGGTTTACAAACAAAGAGATGTTTTATATGGAT
ACTTTCCCATGGTCAAGATTTATTAATGATAGAAAATCTATAATTAATAATAAGTTAAAAAATATAAGAATAGAAGATAG
AGTGAGATATGAATATGAAAAATCATTAAGTAAGGTTCCGCATTTAGAGAATGAAAGTAAAAGAGACTATAGGATGAGAG
AAGTATCATATTTAAATTTAAAATGGTTTATGGTTAATCTACTAAACAGAAAAGATAGAGTAAGTATGTATAATGGTTTA
GAAGTTAGAGTTCCTTTTGCAGATATTAGATTAGTAAATTATGCATTTAATTTACCAGCAGAGATCAAATTATACAAAGG
AAGAGAAAAAGGATTATTAAGAGCGGCTTTAGAAGGTATTCTTCCAAATGAAATTATTTATAGAAAAAAGAGTCCATATC
CTAAAACTCATAATCCTATATATACAGATATAGTTTGTAATATGATGAATGATATATTGAAGAAAAAAGCATCACCTATT
CATGAAATTATTGATGAAGATACAGTAAGAAAAATTGTTGATACAAGAGGAGAAGCGTATAAATCACCTTGGTATGGTCA
ACTTATGACAGGTCCTCAGTTAATAGCGTATTTGATTCAGATAAATACTTGGTTAGAAGAATATAATATAAATATATTAA
TTTAA

Upstream 100 bases:

>100_bases
ATCTTGCACAAAAAAAATATATTAATCATATTTTATAATGCAAAGTGTTAAAAATATAAAATATAAAGTATGAATTAGAT
TTAAATGGGAGATGAACATT

Downstream 100 bases:

>100_bases
GGCTCTGTTTTAAATATATATATTTAAATGTTACTCTAATATAGTCTAATTTAAAAATAAAAAATGATTACCAAATAATA
AATTAGTAATCATTTTTTAT

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MCGIAGLVNFKEDISKYKNILEDMVKTLKKRGPDSQGYYVDKNVLLGHRRLIVVDPDGGKQPMIKIFEGNKYVLIYNGEL
YNTEELRKELKNEGFYFDSYSDTEVLITSYIRWGKNCINKLNGIFAFAIFDEKNNEVFLARDQMGVKPLFYTVYNETLIF
GSEIKTILANSNIKREINEDGLTELFALGPAVTPGKAIFKDIKEIAPANCLLISKTGDIKTWEYWSVEAKEFKETSAEAI
DHTRYLLVDAIKRQLVGDVPICTFLSGGLDSSAISAIAAQEYSKKGKTLTTYSIDYEDNSKYFKSSLFQPTTDEYWAEIM
AKDIGSDHKKITLNHEDLANALKDAVIARDLPGMADIDSSLLLFCKEIRKDFVVGLSGECADEIFAGYPWFTNKEMFYMD
TFPWSRFINDRKSIINNKLKNIRIEDRVRYEYEKSLSKVPHLENESKRDYRMREVSYLNLKWFMVNLLNRKDRVSMYNGL
EVRVPFADIRLVNYAFNLPAEIKLYKGREKGLLRAALEGILPNEIIYRKKSPYPKTHNPIYTDIVCNMMNDILKKKASPI
HEIIDEDTVRKIVDTRGEAYKSPWYGQLMTGPQLIAYLIQINTWLEEYNINILI

Sequences:

>Translated_614_residues
MCGIAGLVNFKEDISKYKNILEDMVKTLKKRGPDSQGYYVDKNVLLGHRRLIVVDPDGGKQPMIKIFEGNKYVLIYNGEL
YNTEELRKELKNEGFYFDSYSDTEVLITSYIRWGKNCINKLNGIFAFAIFDEKNNEVFLARDQMGVKPLFYTVYNETLIF
GSEIKTILANSNIKREINEDGLTELFALGPAVTPGKAIFKDIKEIAPANCLLISKTGDIKTWEYWSVEAKEFKETSAEAI
DHTRYLLVDAIKRQLVGDVPICTFLSGGLDSSAISAIAAQEYSKKGKTLTTYSIDYEDNSKYFKSSLFQPTTDEYWAEIM
AKDIGSDHKKITLNHEDLANALKDAVIARDLPGMADIDSSLLLFCKEIRKDFVVGLSGECADEIFAGYPWFTNKEMFYMD
TFPWSRFINDRKSIINNKLKNIRIEDRVRYEYEKSLSKVPHLENESKRDYRMREVSYLNLKWFMVNLLNRKDRVSMYNGL
EVRVPFADIRLVNYAFNLPAEIKLYKGREKGLLRAALEGILPNEIIYRKKSPYPKTHNPIYTDIVCNMMNDILKKKASPI
HEIIDEDTVRKIVDTRGEAYKSPWYGQLMTGPQLIAYLIQINTWLEEYNINILI
>Mature_614_residues
MCGIAGLVNFKEDISKYKNILEDMVKTLKKRGPDSQGYYVDKNVLLGHRRLIVVDPDGGKQPMIKIFEGNKYVLIYNGEL
YNTEELRKELKNEGFYFDSYSDTEVLITSYIRWGKNCINKLNGIFAFAIFDEKNNEVFLARDQMGVKPLFYTVYNETLIF
GSEIKTILANSNIKREINEDGLTELFALGPAVTPGKAIFKDIKEIAPANCLLISKTGDIKTWEYWSVEAKEFKETSAEAI
DHTRYLLVDAIKRQLVGDVPICTFLSGGLDSSAISAIAAQEYSKKGKTLTTYSIDYEDNSKYFKSSLFQPTTDEYWAEIM
AKDIGSDHKKITLNHEDLANALKDAVIARDLPGMADIDSSLLLFCKEIRKDFVVGLSGECADEIFAGYPWFTNKEMFYMD
TFPWSRFINDRKSIINNKLKNIRIEDRVRYEYEKSLSKVPHLENESKRDYRMREVSYLNLKWFMVNLLNRKDRVSMYNGL
EVRVPFADIRLVNYAFNLPAEIKLYKGREKGLLRAALEGILPNEIIYRKKSPYPKTHNPIYTDIVCNMMNDILKKKASPI
HEIIDEDTVRKIVDTRGEAYKSPWYGQLMTGPQLIAYLIQINTWLEEYNINILI

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=409, Percent_Identity=29.5843520782396, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI168229252, Length=409, Percent_Identity=29.5843520782396, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI168229250, Length=409, Percent_Identity=29.5843520782396, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI296010848, Length=382, Percent_Identity=29.3193717277487, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI296010852, Length=309, Percent_Identity=28.1553398058252, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI296010850, Length=309, Percent_Identity=28.1553398058252, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1786889, Length=399, Percent_Identity=29.0726817042606, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17560178, Length=604, Percent_Identity=28.1456953642384, Blast_Score=152, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI25147557, Length=565, Percent_Identity=27.9646017699115, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71993933, Length=558, Percent_Identity=28.1362007168459, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25147560, Length=340, Percent_Identity=28.8235294117647, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325403, Length=574, Percent_Identity=26.1324041811847, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6321563, Length=647, Percent_Identity=25.5023183925811, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45553209, Length=526, Percent_Identity=27.1863117870722, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70798; Mature: 70798

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGLVNFKEDISKYKNILEDMVKTLKKRGPDSQGYYVDKNVLLGHRRLIVVDPDGGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCEEEEEECCCCCC
QPMIKIFEGNKYVLIYNGELYNTEELRKELKNEGFYFDSYSDTEVLITSYIRWGKNCINK
CCEEEEEECCEEEEEECCCEECHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFAIFDEKNNEVFLARDQMGVKPLFYTVYNETLIFGSEIKTILANSNIKREINED
HCCEEEEEEEECCCCEEEEEECCCCCCCEEEEEECCEEEECHHHHHHHHCCCCHHHCCCC
GLTELFALGPAVTPGKAIFKDIKEIAPANCLLISKTGDIKTWEYWSVEAKEFKETSAEAI
HHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHH
DHTRYLLVDAIKRQLVGDVPICTFLSGGLDSSAISAIAAQEYSKKGKTLTTYSIDYEDNS
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
KYFKSSLFQPTTDEYWAEIMAKDIGSDHKKITLNHEDLANALKDAVIARDLPGMADIDSS
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCHH
LLLFCKEIRKDFVVGLSGECADEIFAGYPWFTNKEMFYMDTFPWSRFINDRKSIINNKLK
HHHHHHHHHHHHEECCCCCHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
NIRIEDRVRYEYEKSLSKVPHLENESKRDYRMREVSYLNLKWFMVNLLNRKDRVSMYNGL
CCEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEEHHHHHHHHHCCCCHHHHCCCC
EVRVPFADIRLVNYAFNLPAEIKLYKGREKGLLRAALEGILPNEIIYRKKSPYPKTHNPI
EEEECHHHEEEEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCHHEECCCCCCCCCCCCC
YTDIVCNMMNDILKKKASPIHEIIDEDTVRKIVDTRGEAYKSPWYGQLMTGPQLIAYLIQ
HHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHH
INTWLEEYNINILI
HHHHHHHCCCEEEC
>Mature Secondary Structure
MCGIAGLVNFKEDISKYKNILEDMVKTLKKRGPDSQGYYVDKNVLLGHRRLIVVDPDGGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCEEEEEECCCCCC
QPMIKIFEGNKYVLIYNGELYNTEELRKELKNEGFYFDSYSDTEVLITSYIRWGKNCINK
CCEEEEEECCEEEEEECCCEECHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHH
LNGIFAFAIFDEKNNEVFLARDQMGVKPLFYTVYNETLIFGSEIKTILANSNIKREINED
HCCEEEEEEEECCCCEEEEEECCCCCCCEEEEEECCEEEECHHHHHHHHCCCCHHHCCCC
GLTELFALGPAVTPGKAIFKDIKEIAPANCLLISKTGDIKTWEYWSVEAKEFKETSAEAI
HHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHH
DHTRYLLVDAIKRQLVGDVPICTFLSGGLDSSAISAIAAQEYSKKGKTLTTYSIDYEDNS
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
KYFKSSLFQPTTDEYWAEIMAKDIGSDHKKITLNHEDLANALKDAVIARDLPGMADIDSS
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCHH
LLLFCKEIRKDFVVGLSGECADEIFAGYPWFTNKEMFYMDTFPWSRFINDRKSIINNKLK
HHHHHHHHHHHHEECCCCCHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
NIRIEDRVRYEYEKSLSKVPHLENESKRDYRMREVSYLNLKWFMVNLLNRKDRVSMYNGL
CCEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEEHHHHHHHHHCCCCHHHHCCCC
EVRVPFADIRLVNYAFNLPAEIKLYKGREKGLLRAALEGILPNEIIYRKKSPYPKTHNPI
EEEECHHHEEEEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCHHEECCCCCCCCCCCCC
YTDIVCNMMNDILKKKASPIHEIIDEDTVRKIVDTRGEAYKSPWYGQLMTGPQLIAYLIQ
HHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHH
INTWLEEYNINILI
HHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]