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The map label for this gene is graS [H]

Identifier: 187934429

GI number: 187934429

Start: 1155440

End: 1156471

Strand: Reverse

Name: graS [H]

Synonym: CLL_A1125

Alternate gene names: 187934429

Gene position: 1156471-1155440 (Counterclockwise)

Preceding gene: 187935627

Following gene: 187933678

Centisome position: 30.43

GC content: 23.16

Gene sequence:

>1032_bases
ATGAGTTTAAAAGAATATATTAATGGAAATAAAACTTTAATACTTTTAAATCTCTTTTATATTATTCTATTAATTGTATT
TTTAAGGTTATTTAGCATCCCACATTTAGCCATATATTTTCTGTGCACCACATTAGTAAGTATATTGGTATTTTATATTT
TGTTTTCATATTTTATAAGAAAGAATTACTACAATAATCTTTCAAGATTTTTTGATGAAAGTGATTTATTTTCTTTAACT
GAGCTTTTAGAAGAACCTACATTTATAGATGGAAAGCCATTTTATACTGCTCTTTTACTTTTAAGTCATGCATTTAATCA
AGAGCTTTTAAAATATTCAAATCAAAGTAATTCTTATCAAGAATACATAGAACAGTGGGTGCATGAAATTAAAACTCCTA
TTTCTTCATTAAAACTAATTATAGAAAATGACAAAGAACTTCTTGGAAAACTTTATGAAGAAGAACTAACTGAACTTCAA
AAAATAGAAAATTATATAGATCAAACTCTTTACTATTCAAGAATGGAATATTCTCAAAATGATTATTTTGTATCAGAATT
TCCAATTATGAAAGTTATAAAAGGTGTTATTATAAAAAACAGAACTCTTATAAATAAAAGCAATATTTCACTTGATATAA
AAAATAGTGGAGAAACAATTTTTTCAGATGAAAAATGGCTTTCTTTTATAATAAATCAAATTATTTTAAATTCTATTAAA
TATTCTAAACCTGAAGGATCATTAATCTCTATTTATGTAATAAAAAATTCAAATAACATTATATTAAATATAAAAGATGA
TGGTATTGGTATAGGGGCTGATGATTTACCCAGAGTATTTTCTAAAGGCTTTACTGGCAGTAATGGAAGACTTAATGAGA
AATCAACAGGTCTAGGGCTTTATTTAGTAAAAAAAATATGTGAAAAACTTGAACATAAAATAACAATTGAATCAGTTAAA
AATGAATACACCCTAGTTTCACTAATATTTCCAAAATCAAATTATAATCAGGTTTTAAAAAGTATAAATTAA

Upstream 100 bases:

>100_bases
ACACTTTAACTGTTAACATAAACAGACTTAGAAAAAAATTAGAAGAGATTAACATTCATGACTTTATTGAAACTAGGCGT
GGATTGGGGTACATAATATC

Downstream 100 bases:

>100_bases
GGAGTTTATAATGGATACACTACTAAAAATTAAGAATTTAACAAAAATTTATGGAGGAAGAAAAAATATTACTAAAGCCT
TAAATGGTCTTAGTTTTTCA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Glycopeptide resistance-associated protein S [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSLKEYINGNKTLILLNLFYIILLIVFLRLFSIPHLAIYFLCTTLVSILVFYILFSYFIRKNYYNNLSRFFDESDLFSLT
ELLEEPTFIDGKPFYTALLLLSHAFNQELLKYSNQSNSYQEYIEQWVHEIKTPISSLKLIIENDKELLGKLYEEELTELQ
KIENYIDQTLYYSRMEYSQNDYFVSEFPIMKVIKGVIIKNRTLINKSNISLDIKNSGETIFSDEKWLSFIINQIILNSIK
YSKPEGSLISIYVIKNSNNIILNIKDDGIGIGADDLPRVFSKGFTGSNGRLNEKSTGLGLYLVKKICEKLEHKITIESVK
NEYTLVSLIFPKSNYNQVLKSIN

Sequences:

>Translated_343_residues
MSLKEYINGNKTLILLNLFYIILLIVFLRLFSIPHLAIYFLCTTLVSILVFYILFSYFIRKNYYNNLSRFFDESDLFSLT
ELLEEPTFIDGKPFYTALLLLSHAFNQELLKYSNQSNSYQEYIEQWVHEIKTPISSLKLIIENDKELLGKLYEEELTELQ
KIENYIDQTLYYSRMEYSQNDYFVSEFPIMKVIKGVIIKNRTLINKSNISLDIKNSGETIFSDEKWLSFIINQIILNSIK
YSKPEGSLISIYVIKNSNNIILNIKDDGIGIGADDLPRVFSKGFTGSNGRLNEKSTGLGLYLVKKICEKLEHKITIESVK
NEYTLVSLIFPKSNYNQVLKSIN
>Mature_342_residues
SLKEYINGNKTLILLNLFYIILLIVFLRLFSIPHLAIYFLCTTLVSILVFYILFSYFIRKNYYNNLSRFFDESDLFSLTE
LLEEPTFIDGKPFYTALLLLSHAFNQELLKYSNQSNSYQEYIEQWVHEIKTPISSLKLIIENDKELLGKLYEEELTELQK
IENYIDQTLYYSRMEYSQNDYFVSEFPIMKVIKGVIIKNRTLINKSNISLDIKNSGETIFSDEKWLSFIINQIILNSIKY
SKPEGSLISIYVIKNSNNIILNIKDDGIGIGADDLPRVFSKGFTGSNGRLNEKSTGLGLYLVKKICEKLEHKITIESVKN
EYTLVSLIFPKSNYNQVLKSIN

Specific function: Member of the two-component regulatory system graR/graS involved in resistance against cationic antimicrobial peptides (CAMPs). GraS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phospha

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=213, Percent_Identity=24.4131455399061, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=218, Percent_Identity=22.0183486238532, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39919; Mature: 39788

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKEYINGNKTLILLNLFYIILLIVFLRLFSIPHLAIYFLCTTLVSILVFYILFSYFIR
CCCHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNYYNNLSRFFDESDLFSLTELLEEPTFIDGKPFYTALLLLSHAFNQELLKYSNQSNSYQ
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EYIEQWVHEIKTPISSLKLIIENDKELLGKLYEEELTELQKIENYIDQTLYYSRMEYSQN
HHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DYFVSEFPIMKVIKGVIIKNRTLINKSNISLDIKNSGETIFSDEKWLSFIINQIILNSIK
CCCHHCCCHHHHHHHHHHCCCEEEECCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHC
YSKPEGSLISIYVIKNSNNIILNIKDDGIGIGADDLPRVFSKGFTGSNGRLNEKSTGLGL
CCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHH
YLVKKICEKLEHKITIESVKNEYTLVSLIFPKSNYNQVLKSIN
HHHHHHHHHHHHHEEHHHHCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
SLKEYINGNKTLILLNLFYIILLIVFLRLFSIPHLAIYFLCTTLVSILVFYILFSYFIR
CCHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNYYNNLSRFFDESDLFSLTELLEEPTFIDGKPFYTALLLLSHAFNQELLKYSNQSNSYQ
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EYIEQWVHEIKTPISSLKLIIENDKELLGKLYEEELTELQKIENYIDQTLYYSRMEYSQN
HHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DYFVSEFPIMKVIKGVIIKNRTLINKSNISLDIKNSGETIFSDEKWLSFIINQIILNSIK
CCCHHCCCHHHHHHHHHHCCCEEEECCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHC
YSKPEGSLISIYVIKNSNNIILNIKDDGIGIGADDLPRVFSKGFTGSNGRLNEKSTGLGL
CCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHH
YLVKKICEKLEHKITIESVKNEYTLVSLIFPKSNYNQVLKSIN
HHHHHHHHHHHHHEEHHHHCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA