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The map label for this gene is prfC [H]

Identifier: 187934298

GI number: 187934298

Start: 3354898

End: 3356493

Strand: Reverse

Name: prfC [H]

Synonym: CLL_A3219

Alternate gene names: 187934298

Gene position: 3356493-3354898 (Counterclockwise)

Preceding gene: 187934652

Following gene: 187933328

Centisome position: 88.32

GC content: 32.46

Gene sequence:

>1596_bases
TTGGCTGATTATATAAAAGAAATTGAAAAGAGAAGAACCTTCGCGATTATTTCCCATCCCGATGCGGGAAAAACTACATT
AACAGAAAAGTTCCTATTATACGGAGGAGCTATTAGACTTGCAGGTTCTGTTAAAGCAAGAAAGGCATCTAAACATGCAG
TTTCTGACTGGATGGAAATTGAAAAACAAAGAGGTATCTCAGTTACTTCATCTGTTATGCAATTTAACTACGATGGTCAT
TGCATTAACATATTAGATACTCCAGGTCACCAAGACTTCTCAGAAGATACATATAGAACACTTATGGCAGCAGATAGTGC
AGTAATGGTTGTAGATGCTGCAAAAGGTATAGAAGATCAAACAAGAAAATTATTTCATGTTGCTTCTTTAAGAGAAATGC
CTATATTTACTTTTGTAAATAAGATGGATAGAGAAGCTAGAGATCCATTTGGTTTACTTGAAGATATCGAAAATGAATTA
GGAATTAAAACTTATCCAATGAACTGGCCAATTGGTTCTGGTAAGGATTTTAAGGGTGTATATGAAAGAGATAATAATAG
AATCATAGCCTTTAATGGAGGAAACCATGGTCAAAATGAAGTTGAAGCCATAGAAGGTTCAGCAGATGATCCTAAATTTA
GAGAAATTTTAGGAGAAGCACTTCATGATAAGTTAATGGAAGATATTGAACTTCTAGACATTGCTGGGGATGATTTAGAT
TTAGAGGCTGTAAGAAGTGGGGAATTAACTCCTGTATTCTTTGGTTCAGCATTAACAAACTTTGGTGTAGAACCATTCTT
AGAACATTTCTTAAAAATGACTACATCACCACTTGCAAGAAACTCATCAGAAGGGGTTATTGACCCATTTGAAGATAAAT
TCTCAGCCTTTGTATTTAAGATTCAAGCTAACATGAATAAAGCTCATAGGGATAGAATTGCATTTATGAGAATTTGTTCA
GGTAAGTTTAACAAAGGTGAAGATGTATATCATATGCAAGGCGGAAAGAAAATTAAATTAGCTCAACCTCAACAATTTAT
GGCTCAAGATAGAGAAATTGTTGAAGAAGCTTATGCAGGAGATATAATTGGGGTATTTGATCCAGGTATATTTTCAATAG
GGGATACATTATGTGCTCCATCAAAGAAATTTAAGTTTGAAGGAATTCCAATATTTGCACCAGAACATTTTGCAAGAGTA
AGACCAGTTGATACTATGAAGAGAAAACAATTTATAAAGGGTGTTACTCAAATTGCTCAAGAAGGTGCAATACAAGTATT
TAAAGAAATTCATATAGGTATGGAAGAAATAATAGTTGGAGTTGTTGGTGTACTTCAATTTGAAGTTCTAGAATACAGAT
TAAAGAATGAATATAATGTAGACATTAAAATGGATAGATTATCTTATAGATATGTAAGATGGATAGAAAATAAAAATATT
GATATGGATAAGTTAAACTTAACTTCAGATACTAAGAAGGTTAAAGATTTAAGAGATAGAAACTTACTTATATTCCAAAA
TGATTGGGGAATAAGTTGGGCTTTAGATCATAATAAAGACGTTATTCTTTCTGATGTAGGTAAGAGTGAAGATTAA

Upstream 100 bases:

>100_bases
GTATACATCAAAATCTTTGATTAAAATATTTTAAAAGCAATAAATTTTAAGAATGTTTATAATATAATTAATGAAATTTT
GAATTAGGGAGGATTTACAT

Downstream 100 bases:

>100_bases
AAATATAGATATTAATAAAAATAGTAGCTGACTTAAATGAGTAAAGCTACTATTTTTTTGCATTAAACTTAGTGTTTGAG
GGCAATATTATAAATTAATA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MADYIKEIEKRRTFAIISHPDAGKTTLTEKFLLYGGAIRLAGSVKARKASKHAVSDWMEIEKQRGISVTSSVMQFNYDGH
CINILDTPGHQDFSEDTYRTLMAADSAVMVVDAAKGIEDQTRKLFHVASLREMPIFTFVNKMDREARDPFGLLEDIENEL
GIKTYPMNWPIGSGKDFKGVYERDNNRIIAFNGGNHGQNEVEAIEGSADDPKFREILGEALHDKLMEDIELLDIAGDDLD
LEAVRSGELTPVFFGSALTNFGVEPFLEHFLKMTTSPLARNSSEGVIDPFEDKFSAFVFKIQANMNKAHRDRIAFMRICS
GKFNKGEDVYHMQGGKKIKLAQPQQFMAQDREIVEEAYAGDIIGVFDPGIFSIGDTLCAPSKKFKFEGIPIFAPEHFARV
RPVDTMKRKQFIKGVTQIAQEGAIQVFKEIHIGMEEIIVGVVGVLQFEVLEYRLKNEYNVDIKMDRLSYRYVRWIENKNI
DMDKLNLTSDTKKVKDLRDRNLLIFQNDWGISWALDHNKDVILSDVGKSED

Sequences:

>Translated_531_residues
MADYIKEIEKRRTFAIISHPDAGKTTLTEKFLLYGGAIRLAGSVKARKASKHAVSDWMEIEKQRGISVTSSVMQFNYDGH
CINILDTPGHQDFSEDTYRTLMAADSAVMVVDAAKGIEDQTRKLFHVASLREMPIFTFVNKMDREARDPFGLLEDIENEL
GIKTYPMNWPIGSGKDFKGVYERDNNRIIAFNGGNHGQNEVEAIEGSADDPKFREILGEALHDKLMEDIELLDIAGDDLD
LEAVRSGELTPVFFGSALTNFGVEPFLEHFLKMTTSPLARNSSEGVIDPFEDKFSAFVFKIQANMNKAHRDRIAFMRICS
GKFNKGEDVYHMQGGKKIKLAQPQQFMAQDREIVEEAYAGDIIGVFDPGIFSIGDTLCAPSKKFKFEGIPIFAPEHFARV
RPVDTMKRKQFIKGVTQIAQEGAIQVFKEIHIGMEEIIVGVVGVLQFEVLEYRLKNEYNVDIKMDRLSYRYVRWIENKNI
DMDKLNLTSDTKKVKDLRDRNLLIFQNDWGISWALDHNKDVILSDVGKSED
>Mature_530_residues
ADYIKEIEKRRTFAIISHPDAGKTTLTEKFLLYGGAIRLAGSVKARKASKHAVSDWMEIEKQRGISVTSSVMQFNYDGHC
INILDTPGHQDFSEDTYRTLMAADSAVMVVDAAKGIEDQTRKLFHVASLREMPIFTFVNKMDREARDPFGLLEDIENELG
IKTYPMNWPIGSGKDFKGVYERDNNRIIAFNGGNHGQNEVEAIEGSADDPKFREILGEALHDKLMEDIELLDIAGDDLDL
EAVRSGELTPVFFGSALTNFGVEPFLEHFLKMTTSPLARNSSEGVIDPFEDKFSAFVFKIQANMNKAHRDRIAFMRICSG
KFNKGEDVYHMQGGKKIKLAQPQQFMAQDREIVEEAYAGDIIGVFDPGIFSIGDTLCAPSKKFKFEGIPIFAPEHFARVR
PVDTMKRKQFIKGVTQIAQEGAIQVFKEIHIGMEEIIVGVVGVLQFEVLEYRLKNEYNVDIKMDRLSYRYVRWIENKNID
MDKLNLTSDTKKVKDLRDRNLLIFQNDWGISWALDHNKDVILSDVGKSED

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=481, Percent_Identity=30.7692307692308, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI19923640, Length=532, Percent_Identity=26.5037593984962, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=29.923273657289, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI25306287, Length=295, Percent_Identity=32.8813559322034, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=33.7579617834395, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=33.0935251798561, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=29.5302013422819, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=33.6206896551724, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=33.6206896551724, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=33.6206896551724, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=502, Percent_Identity=49.003984063745, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1789738, Length=511, Percent_Identity=28.5714285714286, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1788922, Length=178, Percent_Identity=34.2696629213483, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994988, Length=142, Percent_Identity=36.6197183098592, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=498, Percent_Identity=27.9116465863454, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17556745, Length=498, Percent_Identity=24.0963855421687, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=30.2631578947368, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=31.055900621118, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=143, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=143, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=500, Percent_Identity=28.2, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=522, Percent_Identity=25.8620689655172, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6323320, Length=142, Percent_Identity=29.5774647887324, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=215, Percent_Identity=26.046511627907, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=215, Percent_Identity=26.046511627907, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24582462, Length=489, Percent_Identity=28.4253578732106, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI221458488, Length=514, Percent_Identity=24.9027237354086, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI78706572, Length=171, Percent_Identity=35.672514619883, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24585711, Length=219, Percent_Identity=27.3972602739726, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585713, Length=219, Percent_Identity=27.3972602739726, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585709, Length=219, Percent_Identity=27.3972602739726, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60236; Mature: 60105

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYIKEIEKRRTFAIISHPDAGKTTLTEKFLLYGGAIRLAGSVKARKASKHAVSDWMEI
CCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHH
EKQRGISVTSSVMQFNYDGHCINILDTPGHQDFSEDTYRTLMAADSAVMVVDAAKGIEDQ
HHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHH
TRKLFHVASLREMPIFTFVNKMDREARDPFGLLEDIENELGIKTYPMNWPIGSGKDFKGV
HHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCC
YERDNNRIIAFNGGNHGQNEVEAIEGSADDPKFREILGEALHDKLMEDIELLDIAGDDLD
EECCCCEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
LEAVRSGELTPVFFGSALTNFGVEPFLEHFLKMTTSPLARNSSEGVIDPFEDKFSAFVFK
HHHHCCCCCCEEEEHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHEEEEEE
IQANMNKAHRDRIAFMRICSGKFNKGEDVYHMQGGKKIKLAQPQQFMAQDREIVEEAYAG
EECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHCC
DIIGVFDPGIFSIGDTLCAPSKKFKFEGIPIFAPEHFARVRPVDTMKRKQFIKGVTQIAQ
CEEEECCCCHHHCCHHHCCCCCCCEECCCEEECCHHHHHCCCHHHHHHHHHHHHHHHHHH
EGAIQVFKEIHIGMEEIIVGVVGVLQFEVLEYRLKNEYNVDIKMDRLSYRYVRWIENKNI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCC
DMDKLNLTSDTKKVKDLRDRNLLIFQNDWGISWALDHNKDVILSDVGKSED
CCCEECCCCHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEEEECCCCCCC
>Mature Secondary Structure 
ADYIKEIEKRRTFAIISHPDAGKTTLTEKFLLYGGAIRLAGSVKARKASKHAVSDWMEI
CHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHH
EKQRGISVTSSVMQFNYDGHCINILDTPGHQDFSEDTYRTLMAADSAVMVVDAAKGIEDQ
HHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHH
TRKLFHVASLREMPIFTFVNKMDREARDPFGLLEDIENELGIKTYPMNWPIGSGKDFKGV
HHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCC
YERDNNRIIAFNGGNHGQNEVEAIEGSADDPKFREILGEALHDKLMEDIELLDIAGDDLD
EECCCCEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
LEAVRSGELTPVFFGSALTNFGVEPFLEHFLKMTTSPLARNSSEGVIDPFEDKFSAFVFK
HHHHCCCCCCEEEEHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHEEEEEE
IQANMNKAHRDRIAFMRICSGKFNKGEDVYHMQGGKKIKLAQPQQFMAQDREIVEEAYAG
EECCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHCC
DIIGVFDPGIFSIGDTLCAPSKKFKFEGIPIFAPEHFARVRPVDTMKRKQFIKGVTQIAQ
CEEEECCCCHHHCCHHHCCCCCCCEECCCEEECCHHHHHCCCHHHHHHHHHHHHHHHHHH
EGAIQVFKEIHIGMEEIIVGVVGVLQFEVLEYRLKNEYNVDIKMDRLSYRYVRWIENKNI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCC
DMDKLNLTSDTKKVKDLRDRNLLIFQNDWGISWALDHNKDVILSDVGKSED
CCCEECCCCHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA