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The map label for this gene is rfbD [H]

Identifier: 187934268

GI number: 187934268

Start: 3404272

End: 3405114

Strand: Reverse

Name: rfbD [H]

Synonym: CLL_A3249

Alternate gene names: 187934268

Gene position: 3405114-3404272 (Counterclockwise)

Preceding gene: 187933644

Following gene: 255987641

Centisome position: 89.6

GC content: 28.35

Gene sequence:

>843_bases
ATGATTTTAGTTACTGGAGTAAATGGTCAACTAGGATATGATGTTGTAAAAGAATTAAATAAAAGAGATATTGAATGTTT
AGGAATAGATATAAAAGATTTAGACATAACAGATGATAAAGCTGTTAATGAATATATTTTAAATTTAAAGCCAGAATGTG
TTATACATTGTGCTGCATATACAGCTGTTGATAAGGCAGAAGATGAAGAAGAAATTTGTACTAAAGTGAATGTATATGGA
ACAGAAAATATAGCAAAGGCATGTAAAGAAATAGATACAAAGATGGTTTACATATCGACTGATTATGTATTTGATGGATT
AGGTGATAAACCATTTGAGGTAGATGGAAATATTAATCCGTTATCAGTTTATGGAAAAACTAAATATAAAGGTGAACTAA
AAGTAAAAGAAATTTTAGATAAATATTTTATAGTTAGAATTTCATGGGTATTTGGTATTAATGGAAATAATTTCATTAAT
ATAATGCTTAGATTGGGAGCAGAAAAAGAAAGTTTAAATGTAGTTTCTGATCAAATTGGGAGTCCAACGTATACAGCTGA
TTTAGCACCACTTTTATGTGATATGGTTATATCAGAAAAATATGGAGTTTATCATGCAACCAATGAAAGGACATGCTCTT
GGGCAGAATTTGCAGAAGAAATTATGAAAATGGCAGGATTAAATTGTAAAATTAATTACATTCAAACAAGTGAATACCCA
ACAAAGGCAGTTAGACCATTTAATTCTAGATTGTCTAAGAAATCATTAGTTGATAATGGATTTGAATTAATGCCTGAGTG
GAAAAATGCTTTGGAAAGATATTTAACAGAATTAAGAGGATAA

Upstream 100 bases:

>100_bases
TTCTATATTAATAGAAACCTTTTTACCGTTTATACCTAATACTATATTTAAATATATTATTAATATGACTAAGAAGGGTT
TACTATAAAGGAGGAAAAAT

Downstream 100 bases:

>100_bases
GTTTAATATGAATAAAAGGAAGTATATAAAATATGTAATATTGATATTTTTAGTGGTTATATCAAATATAGTGGTATTTA
ATTATTGTAACTTAGACAAT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MILVTGVNGQLGYDVVKELNKRDIECLGIDIKDLDITDDKAVNEYILNLKPECVIHCAAYTAVDKAEDEEEICTKVNVYG
TENIAKACKEIDTKMVYISTDYVFDGLGDKPFEVDGNINPLSVYGKTKYKGELKVKEILDKYFIVRISWVFGINGNNFIN
IMLRLGAEKESLNVVSDQIGSPTYTADLAPLLCDMVISEKYGVYHATNERTCSWAEFAEEIMKMAGLNCKINYIQTSEYP
TKAVRPFNSRLSKKSLVDNGFELMPEWKNALERYLTELRG

Sequences:

>Translated_280_residues
MILVTGVNGQLGYDVVKELNKRDIECLGIDIKDLDITDDKAVNEYILNLKPECVIHCAAYTAVDKAEDEEEICTKVNVYG
TENIAKACKEIDTKMVYISTDYVFDGLGDKPFEVDGNINPLSVYGKTKYKGELKVKEILDKYFIVRISWVFGINGNNFIN
IMLRLGAEKESLNVVSDQIGSPTYTADLAPLLCDMVISEKYGVYHATNERTCSWAEFAEEIMKMAGLNCKINYIQTSEYP
TKAVRPFNSRLSKKSLVDNGFELMPEWKNALERYLTELRG
>Mature_280_residues
MILVTGVNGQLGYDVVKELNKRDIECLGIDIKDLDITDDKAVNEYILNLKPECVIHCAAYTAVDKAEDEEEICTKVNVYG
TENIAKACKEIDTKMVYISTDYVFDGLGDKPFEVDGNINPLSVYGKTKYKGELKVKEILDKYFIVRISWVFGINGNNFIN
IMLRLGAEKESLNVVSDQIGSPTYTADLAPLLCDMVISEKYGVYHATNERTCSWAEFAEEIMKMAGLNCKINYIQTSEYP
TKAVRPFNSRLSKKSLVDNGFELMPEWKNALERYLTELRG

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=267, Percent_Identity=26.5917602996255, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI11034825, Length=267, Percent_Identity=26.5917602996255, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788352, Length=292, Percent_Identity=35.6164383561644, Blast_Score=185, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 31643; Mature: 31643

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGVNGQLGYDVVKELNKRDIECLGIDIKDLDITDDKAVNEYILNLKPECVIHCAAY
CEEEEECCCCCCHHHHHHHCCCCCEEEECEEEECCCCCCHHHHHHHHCCCCHHEEEEHHH
TAVDKAEDEEEICTKVNVYGTENIAKACKEIDTKMVYISTDYVFDGLGDKPFEVDGNINP
HHHHCCCCHHHHHHEEEECCCHHHHHHHHHHCCEEEEEEECEEECCCCCCCEEECCCCCE
LSVYGKTKYKGELKVKEILDKYFIVRISWVFGINGNNFINIMLRLGAEKESLNVVSDQIG
EEEECCCCCCCCCHHHHHHHHHEEEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHCC
SPTYTADLAPLLCDMVISEKYGVYHATNERTCSWAEFAEEIMKMAGLNCKINYIQTSEYP
CCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCC
TKAVRPFNSRLSKKSLVDNGFELMPEWKNALERYLTELRG
HHHHCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILVTGVNGQLGYDVVKELNKRDIECLGIDIKDLDITDDKAVNEYILNLKPECVIHCAAY
CEEEEECCCCCCHHHHHHHCCCCCEEEECEEEECCCCCCHHHHHHHHCCCCHHEEEEHHH
TAVDKAEDEEEICTKVNVYGTENIAKACKEIDTKMVYISTDYVFDGLGDKPFEVDGNINP
HHHHCCCCHHHHHHEEEECCCHHHHHHHHHHCCEEEEEEECEEECCCCCCCEEECCCCCE
LSVYGKTKYKGELKVKEILDKYFIVRISWVFGINGNNFINIMLRLGAEKESLNVVSDQIG
EEEECCCCCCCCCHHHHHHHHHEEEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHCC
SPTYTADLAPLLCDMVISEKYGVYHATNERTCSWAEFAEEIMKMAGLNCKINYIQTSEYP
CCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCC
TKAVRPFNSRLSKKSLVDNGFELMPEWKNALERYLTELRG
HHHHCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]