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The map label for this gene is ytzG [H]

Identifier: 187933363

GI number: 187933363

Start: 3495287

End: 3496081

Strand: Reverse

Name: ytzG [H]

Synonym: CLL_A3322

Alternate gene names: 187933363

Gene position: 3496081-3495287 (Counterclockwise)

Preceding gene: 187934768

Following gene: 187934355

Centisome position: 91.99

GC content: 27.17

Gene sequence:

>795_bases
ATGAGATTAGATAAATTTTTGGCAGAATCAGCTGTTGGTAGAAGAAAAATTGTTAGAACTTATATTAAAGATGGAATGGT
AAAGGTTAATGGTGAAATTATAACTGAACCTTCAATAGATATTAATGAAAAACTTGATGATGTAGAATACCTTGAAAAAA
AAGTTGTTCATACGGGCAAGGTATATTATATGTTTAATAAACCATCAGGATGCATTACTGCAACAAAAGATAATATTCAT
AGAACAGTATTTGATTATTTTGATGAAGATATGAATGGAGTATTTCATGTTGGAAGACTAGATAAAGATACAGAAGGTTT
ATTATTACTTACTAATGATGGTGAGTTTGAACATAAAGTAATGCATCCAGAAAAACATGTTGAAAAAACTTATTTTTTTT
GGGCATTAGGTTCTTTAGATGATGAAGATAAAAAGCAATTACAGGAAGGTGTTTTTATTGGAAGTAATGAAGCATTAACA
AAGCCTGCAAAAATAGATATACATAAATCAGGATCATATATAGAATTTAAAGAAGAAATTCCTATTAATAGTTTAAAGAA
TAAAGATCATCAGCCTGTTGTTTCAGGATATCTTACTATTTCAGAGGGGCAAAAACATCAGGTTAAACGAATGTTAAAGG
CAGTAGGTTGTTATGTAATATATTTGAAAAGAATATCTATTGGTGGATTAAAGTTAGATGAATCTTTAAAAATGGGTGAT
TATAGGGATTTAACAAAGAAAGAAATTAAAAGAATATTTGAAATAAATGATATAAATAATTTAAAAATGAGGTGA

Upstream 100 bases:

>100_bases
TAGAATATGTAGAAGATTAAATAAAAATTATGCAGTTAAATTATACAATGATTATCTTGAGAGATATGAAAATTTACAAA
GGAAGCGAAATTATAAACGT

Downstream 100 bases:

>100_bases
TTATATGAAAAACAACGTTTCATTTGGGGCATCCTTACTTAAATTCAGCGACAATAGCTGGAATATTAAGAATGACATTT
AAGAGATATGATAGTGAGCA

Product: RNA pseudouridine synthase family protein

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRLDKFLAESAVGRRKIVRTYIKDGMVKVNGEIITEPSIDINEKLDDVEYLEKKVVHTGKVYYMFNKPSGCITATKDNIH
RTVFDYFDEDMNGVFHVGRLDKDTEGLLLLTNDGEFEHKVMHPEKHVEKTYFFWALGSLDDEDKKQLQEGVFIGSNEALT
KPAKIDIHKSGSYIEFKEEIPINSLKNKDHQPVVSGYLTISEGQKHQVKRMLKAVGCYVIYLKRISIGGLKLDESLKMGD
YRDLTKKEIKRIFEINDINNLKMR

Sequences:

>Translated_264_residues
MRLDKFLAESAVGRRKIVRTYIKDGMVKVNGEIITEPSIDINEKLDDVEYLEKKVVHTGKVYYMFNKPSGCITATKDNIH
RTVFDYFDEDMNGVFHVGRLDKDTEGLLLLTNDGEFEHKVMHPEKHVEKTYFFWALGSLDDEDKKQLQEGVFIGSNEALT
KPAKIDIHKSGSYIEFKEEIPINSLKNKDHQPVVSGYLTISEGQKHQVKRMLKAVGCYVIYLKRISIGGLKLDESLKMGD
YRDLTKKEIKRIFEINDINNLKMR
>Mature_264_residues
MRLDKFLAESAVGRRKIVRTYIKDGMVKVNGEIITEPSIDINEKLDDVEYLEKKVVHTGKVYYMFNKPSGCITATKDNIH
RTVFDYFDEDMNGVFHVGRLDKDTEGLLLLTNDGEFEHKVMHPEKHVEKTYFFWALGSLDDEDKKQLQEGVFIGSNEALT
KPAKIDIHKSGSYIEFKEEIPINSLKNKDHQPVVSGYLTISEGQKHQVKRMLKAVGCYVIYLKRISIGGLKLDESLKMGD
YRDLTKKEIKRIFEINDINNLKMR

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=254, Percent_Identity=36.6141732283465, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787524, Length=255, Percent_Identity=30.1960784313725, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081838, Length=184, Percent_Identity=29.8913043478261, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790453, Length=259, Percent_Identity=26.6409266409266, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 30481; Mature: 30481

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFLAESAVGRRKIVRTYIKDGMVKVNGEIITEPSIDINEKLDDVEYLEKKVVHTGK
CCHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEECCCCCHHHCCHHHHHHHHHHHCCCE
VYYMFNKPSGCITATKDNIHRTVFDYFDEDMNGVFHVGRLDKDTEGLLLLTNDGEFEHKV
EEEEEECCCCCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCHHC
MHPEKHVEKTYFFWALGSLDDEDKKQLQEGVFIGSNEALTKPAKIDIHKSGSYIEFKEEI
CCCHHHHHHEEEEEEECCCCCHHHHHHHCCEEECCCCCCCCCCEEEEECCCCEEEEECCC
PINSLKNKDHQPVVSGYLTISEGQKHQVKRMLKAVGCYVIYLKRISIGGLKLDESLKMGD
CCHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHEEEEHHCCCCEEECCCCCCCC
YRDLTKKEIKRIFEINDINNLKMR
CHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRLDKFLAESAVGRRKIVRTYIKDGMVKVNGEIITEPSIDINEKLDDVEYLEKKVVHTGK
CCHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEECCCCCHHHCCHHHHHHHHHHHCCCE
VYYMFNKPSGCITATKDNIHRTVFDYFDEDMNGVFHVGRLDKDTEGLLLLTNDGEFEHKV
EEEEEECCCCCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCHHC
MHPEKHVEKTYFFWALGSLDDEDKKQLQEGVFIGSNEALTKPAKIDIHKSGSYIEFKEEI
CCCHHHHHHEEEEEEECCCCCHHHHHHHCCEEECCCCCCCCCCEEEEECCCCEEEEECCC
PINSLKNKDHQPVVSGYLTISEGQKHQVKRMLKAVGCYVIYLKRISIGGLKLDESLKMGD
CCHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHEEEEHHCCCCEEECCCCCCCC
YRDLTKKEIKRIFEINDINNLKMR
CHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]