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The map label for this gene is topB [H]

Identifier: 187932910

GI number: 187932910

Start: 2744479

End: 2746635

Strand: Reverse

Name: topB [H]

Synonym: CLL_A2636

Alternate gene names: 187932910

Gene position: 2746635-2744479 (Counterclockwise)

Preceding gene: 187935765

Following gene: 187933582

Centisome position: 72.27

GC content: 28.56

Gene sequence:

>2157_bases
ATGGCAAAGGTAATTATTGCAGAAAAGCCTTCTGTTGCAAAAAATATTGCAGATGCGTTAAATATAAAAACTAGAAAAGA
TGGTTATTTTAAAGGTGAAGAGTACTATATCACTTGGGCCTTTGGTCATCTTTTGCAATTATATGATGCAAAAGATTATG
ATGAAAATATGAAGGGGTGGAGAATGGAGAAGTTTCCATTTATTCCTGAATCTTTTCAGTATAAAGTAAAGTGTGATAAT
ATTGATAGAACAGTTGAAGATAAAGGCGCTGCAAAACAAATTAACATAATAAAGTCCTTAATAGAGCAAGAAGATGTAGA
TGGAGTTATATCTGCTACGGATTTTGATAGAGAAGGGCAAGTAATTGCAGATGAATTGTTTAATTATTTAAATATTCAAA
AGCCAATATACAGATTATTGTTAAATGAATGGACTCCAGATGAAGTAAAAAAAGGGATGGGAAGTTTAAAAGAAAATAAA
GAAATGCAATTTTTGCAAGATGCAGGAATAGGCAGGCAATGGACAGATTGGATTATTGGAATAAATTTAACTTCTGTTAG
TACATTGAAGTATAAATTTGAAGAAAACAAAACTATTAACATAGGTAGAGTATTATTACCAACGTTAAAGATAATATATG
ACAGAGATAAAGAAATAGAGAATTTTGAAGCTACTACTTACTATAAATTAACTTCTACATTTAAAAATTCAAGTAATGAA
GAATTTGATGGGTTATATTATGAAAAGGAATCAGAAAAGTTTGAAGATAAAAGTGTTTTAGATAATTTAGTAAAATTACT
AGATGGAACTAATGCAACTATAATAGACAAACAAACTGAAAAAAAGAAAGATTATCCACCATATCTTTTCAACTTATCTA
ATTTACAAGGATATATAACAAGTAAGTATAAAGGATGGACATCAGATAAAGTATTAAAGGTTGCACAAAGCCTTTATGAA
AAGAAATATACAACTTATCCTAGAACTGCAAGCGTGGTCTTAGAAGAGAGCTTAGAGGACAGAGCAAGGAAAGTTTTAAA
TACATTAAAGATAGGATTACCTTACGAAAAAGATATTAAGTTTATAAAAACTAAAAGAGTATTTGATAATTCAAAGGTTG
AAAGCCATAGTGCAATAACACCAACATATATTAAACCAAATGGACTTAGTAAAGATGAAGAAATTGTTTATAATGCTATT
AAAAATAGATTTATAATGCAGTTTATGCCTATTGCAGAGTTTGAAGAAACAAAGATAAGATTAAAACCATCTAATGAGGA
AATACAAGGAGAGTTTATATCAAAAGGAAAAGTTCAACTTGTAGAGGGATGGAGAGTAGTTGAAAAAATAGATACTAAAG
ATACAATACTGCCTATGGTTAATGTAGACGAAAAAGTAGATATAGTATCAAATAAAGTAAATAAGGTAACTAAAAAACCA
CCAAAGCATCATACAGAAAAAACATTACTTAGAGTTATGGAAACTTGCGGTAAGGGGATAGAAGGAAAAGAAGATTCTGA
AGAGATGATGCAATCTATATTAAATGGATTTAGTATAGGAACTCCAGCAACTAGAGCAGAAACTATCAAAAAACTTAAAG
ATATCGGATACATAGGAACTAAAGGAAAAAGTCTTATATGTACGCAATTAGGCATAAATATAGTTGAAATATTTCCAGTT
CAAGAACTTTTGGACCTTGAGTATACAGGTAGACTTGAAAGTACATTATCTGATATAGAAAAAGGCAAATTTAAAAAGTC
TGATTTTATGAGTTTGATATATGATTTTACGAATAATGCTGTTGATAAAATCAAAAAGGATACATCAGCTTTATCTAAAT
TTAAAGTGGAACTTCCAGAAGGTGCAGAGGAAATTGGAAAATGTCCTGTATGTGGAAATTCAGTCATTGAAAATGAAAAG
AACTTTGGATGTACAAATTGGAAAAATGGATGTAAGTATACCATATGGAAAGACGATAAATATATATCATCCTTTGGTAA
AAAAGTTTCTAAAGAAATGGTTGTTTTATTACTTAAGAATGGAAAAGTAGGATTTAGAAATTTAAAGAGTAAAAAAGGAA
ATACATTTTCAGCATATTTTAGATATGAAAAAAATGAGAAAACAGGATATTTTAATTGGAATATTGAATTTATATAA

Upstream 100 bases:

>100_bases
CAGACGGGAATGCAGTAAGCTAGCCTTATTTTTGTTTTAATGTTAATATATATGTTGGTAAAAGATAATAAATAGAGTAG
TGATTTTAAGGAGGTAAGGC

Downstream 100 bases:

>100_bases
AAGGCTCTGTTTTAAATATGTATATATCAACACGCTACTAAAACATAGCCATTTAAAAATAGACGCTGTAAAATTAACTT
TAAGTTGATTTTACAACGTC

Product: DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MAKVIIAEKPSVAKNIADALNIKTRKDGYFKGEEYYITWAFGHLLQLYDAKDYDENMKGWRMEKFPFIPESFQYKVKCDN
IDRTVEDKGAAKQINIIKSLIEQEDVDGVISATDFDREGQVIADELFNYLNIQKPIYRLLLNEWTPDEVKKGMGSLKENK
EMQFLQDAGIGRQWTDWIIGINLTSVSTLKYKFEENKTINIGRVLLPTLKIIYDRDKEIENFEATTYYKLTSTFKNSSNE
EFDGLYYEKESEKFEDKSVLDNLVKLLDGTNATIIDKQTEKKKDYPPYLFNLSNLQGYITSKYKGWTSDKVLKVAQSLYE
KKYTTYPRTASVVLEESLEDRARKVLNTLKIGLPYEKDIKFIKTKRVFDNSKVESHSAITPTYIKPNGLSKDEEIVYNAI
KNRFIMQFMPIAEFEETKIRLKPSNEEIQGEFISKGKVQLVEGWRVVEKIDTKDTILPMVNVDEKVDIVSNKVNKVTKKP
PKHHTEKTLLRVMETCGKGIEGKEDSEEMMQSILNGFSIGTPATRAETIKKLKDIGYIGTKGKSLICTQLGINIVEIFPV
QELLDLEYTGRLESTLSDIEKGKFKKSDFMSLIYDFTNNAVDKIKKDTSALSKFKVELPEGAEEIGKCPVCGNSVIENEK
NFGCTNWKNGCKYTIWKDDKYISSFGKKVSKEMVVLLLKNGKVGFRNLKSKKGNTFSAYFRYEKNEKTGYFNWNIEFI

Sequences:

>Translated_718_residues
MAKVIIAEKPSVAKNIADALNIKTRKDGYFKGEEYYITWAFGHLLQLYDAKDYDENMKGWRMEKFPFIPESFQYKVKCDN
IDRTVEDKGAAKQINIIKSLIEQEDVDGVISATDFDREGQVIADELFNYLNIQKPIYRLLLNEWTPDEVKKGMGSLKENK
EMQFLQDAGIGRQWTDWIIGINLTSVSTLKYKFEENKTINIGRVLLPTLKIIYDRDKEIENFEATTYYKLTSTFKNSSNE
EFDGLYYEKESEKFEDKSVLDNLVKLLDGTNATIIDKQTEKKKDYPPYLFNLSNLQGYITSKYKGWTSDKVLKVAQSLYE
KKYTTYPRTASVVLEESLEDRARKVLNTLKIGLPYEKDIKFIKTKRVFDNSKVESHSAITPTYIKPNGLSKDEEIVYNAI
KNRFIMQFMPIAEFEETKIRLKPSNEEIQGEFISKGKVQLVEGWRVVEKIDTKDTILPMVNVDEKVDIVSNKVNKVTKKP
PKHHTEKTLLRVMETCGKGIEGKEDSEEMMQSILNGFSIGTPATRAETIKKLKDIGYIGTKGKSLICTQLGINIVEIFPV
QELLDLEYTGRLESTLSDIEKGKFKKSDFMSLIYDFTNNAVDKIKKDTSALSKFKVELPEGAEEIGKCPVCGNSVIENEK
NFGCTNWKNGCKYTIWKDDKYISSFGKKVSKEMVVLLLKNGKVGFRNLKSKKGNTFSAYFRYEKNEKTGYFNWNIEFI
>Mature_717_residues
AKVIIAEKPSVAKNIADALNIKTRKDGYFKGEEYYITWAFGHLLQLYDAKDYDENMKGWRMEKFPFIPESFQYKVKCDNI
DRTVEDKGAAKQINIIKSLIEQEDVDGVISATDFDREGQVIADELFNYLNIQKPIYRLLLNEWTPDEVKKGMGSLKENKE
MQFLQDAGIGRQWTDWIIGINLTSVSTLKYKFEENKTINIGRVLLPTLKIIYDRDKEIENFEATTYYKLTSTFKNSSNEE
FDGLYYEKESEKFEDKSVLDNLVKLLDGTNATIIDKQTEKKKDYPPYLFNLSNLQGYITSKYKGWTSDKVLKVAQSLYEK
KYTTYPRTASVVLEESLEDRARKVLNTLKIGLPYEKDIKFIKTKRVFDNSKVESHSAITPTYIKPNGLSKDEEIVYNAIK
NRFIMQFMPIAEFEETKIRLKPSNEEIQGEFISKGKVQLVEGWRVVEKIDTKDTILPMVNVDEKVDIVSNKVNKVTKKPP
KHHTEKTLLRVMETCGKGIEGKEDSEEMMQSILNGFSIGTPATRAETIKKLKDIGYIGTKGKSLICTQLGINIVEIFPVQ
ELLDLEYTGRLESTLSDIEKGKFKKSDFMSLIYDFTNNAVDKIKKDTSALSKFKVELPEGAEEIGKCPVCGNSVIENEKN
FGCTNWKNGCKYTIWKDDKYISSFGKKVSKEMVVLLLKNGKVGFRNLKSKKGNTFSAYFRYEKNEKTGYFNWNIEFI

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=735, Percent_Identity=24.4897959183673, Blast_Score=123, Evalue=8e-28,
Organism=Homo sapiens, GI4507635, Length=542, Percent_Identity=23.0627306273063, Blast_Score=77, Evalue=8e-14,
Organism=Escherichia coli, GI1788061, Length=634, Percent_Identity=28.0757097791798, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1787529, Length=599, Percent_Identity=23.5392320534224, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=632, Percent_Identity=22.9430379746835, Blast_Score=100, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17555378, Length=600, Percent_Identity=23.5, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 82672; Mature: 82540

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVIIAEKPSVAKNIADALNIKTRKDGYFKGEEYYITWAFGHLLQLYDAKDYDENMKGW
CCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCC
RMEKFPFIPESFQYKVKCDNIDRTVEDKGAAKQINIIKSLIEQEDVDGVISATDFDREGQ
EECCCCCCCCCCEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
VIADELFNYLNIQKPIYRLLLNEWTPDEVKKGMGSLKENKEMQFLQDAGIGRQWTDWIIG
CHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHCCHHCCCHHHHHHHCCCCCCCCCEEEE
INLTSVSTLKYKFEENKTINIGRVLLPTLKIIYDRDKEIENFEATTYYKLTSTFKNSSNE
EEEEEEEEEEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCCC
EFDGLYYEKESEKFEDKSVLDNLVKLLDGTNATIIDKQTEKKKDYPPYLFNLSNLQGYIT
CCCCEEEECCCHHCHHHHHHHHHHHHHCCCCCEEECCCHHCCCCCCCEEEECCCCCEEEE
SKYKGWTSDKVLKVAQSLYEKKYTTYPRTASVVLEESLEDRARKVLNTLKIGLPYEKDIK
ECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FIKTKRVFDNSKVESHSAITPTYIKPNGLSKDEEIVYNAIKNRFIMQFMPIAEFEETKIR
HHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LKPSNEEIQGEFISKGKVQLVEGWRVVEKIDTKDTILPMVNVDEKVDIVSNKVNKVTKKP
ECCCCCCCCCHHHCCCCEEEEHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCC
PKHHTEKTLLRVMETCGKGIEGKEDSEEMMQSILNGFSIGTPATRAETIKKLKDIGYIGT
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCC
KGKSLICTQLGINIVEIFPVQELLDLEYTGRLESTLSDIEKGKFKKSDFMSLIYDFTNNA
CCCEEEEEHHCCCEEEEECHHHHHCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VDKIKKDTSALSKFKVELPEGAEEIGKCPVCGNSVIENEKNFGCTNWKNGCKYTIWKDDK
HHHHHHHHHHHHHEEEECCCCHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCHH
YISSFGKKVSKEMVVLLLKNGKVGFRNLKSKKGNTFSAYFRYEKNEKTGYFNWNIEFI
HHHHHHHHHHHHHEEEEEECCCCCHHHHHCCCCCEEEEEEEEECCCCCEEEEEEEEEC
>Mature Secondary Structure 
AKVIIAEKPSVAKNIADALNIKTRKDGYFKGEEYYITWAFGHLLQLYDAKDYDENMKGW
CEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCC
RMEKFPFIPESFQYKVKCDNIDRTVEDKGAAKQINIIKSLIEQEDVDGVISATDFDREGQ
EECCCCCCCCCCEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
VIADELFNYLNIQKPIYRLLLNEWTPDEVKKGMGSLKENKEMQFLQDAGIGRQWTDWIIG
CHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHCCHHCCCHHHHHHHCCCCCCCCCEEEE
INLTSVSTLKYKFEENKTINIGRVLLPTLKIIYDRDKEIENFEATTYYKLTSTFKNSSNE
EEEEEEEEEEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCCC
EFDGLYYEKESEKFEDKSVLDNLVKLLDGTNATIIDKQTEKKKDYPPYLFNLSNLQGYIT
CCCCEEEECCCHHCHHHHHHHHHHHHHCCCCCEEECCCHHCCCCCCCEEEECCCCCEEEE
SKYKGWTSDKVLKVAQSLYEKKYTTYPRTASVVLEESLEDRARKVLNTLKIGLPYEKDIK
ECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FIKTKRVFDNSKVESHSAITPTYIKPNGLSKDEEIVYNAIKNRFIMQFMPIAEFEETKIR
HHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LKPSNEEIQGEFISKGKVQLVEGWRVVEKIDTKDTILPMVNVDEKVDIVSNKVNKVTKKP
ECCCCCCCCCHHHCCCCEEEEHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCC
PKHHTEKTLLRVMETCGKGIEGKEDSEEMMQSILNGFSIGTPATRAETIKKLKDIGYIGT
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCC
KGKSLICTQLGINIVEIFPVQELLDLEYTGRLESTLSDIEKGKFKKSDFMSLIYDFTNNA
CCCEEEEEHHCCCEEEEECHHHHHCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VDKIKKDTSALSKFKVELPEGAEEIGKCPVCGNSVIENEKNFGCTNWKNGCKYTIWKDDK
HHHHHHHHHHHHHEEEECCCCHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECCHH
YISSFGKKVSKEMVVLLLKNGKVGFRNLKSKKGNTFSAYFRYEKNEKTGYFNWNIEFI
HHHHHHHHHHHHHEEEEEECCCCCHHHHHCCCCCEEEEEEEEECCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA