Click here to switch to the map view.

The map label for this gene is alaXL [H]

Identifier: 187932858

GI number: 187932858

Start: 1836119

End: 1837318

Strand: Reverse

Name: alaXL [H]

Synonym: CLL_A1751

Alternate gene names: 187932858

Gene position: 1837318-1836119 (Counterclockwise)

Preceding gene: 187934874

Following gene: 187934480

Centisome position: 48.35

GC content: 25.42

Gene sequence:

>1200_bases
ATGCAAAAACTTTACTACGAAGATCAATATTTAAAGAAATTCACTGCTGAAATAGAACAAGTCAAGGAAGTAGGTAATGA
GTTTCATATAGTTTTAAATAAAACTGCATTTTTCCCAGGGGGAGGAGGGCAAAGACATGATTTGGGCAAAATTGATGAAA
TTGAAGTGTTAAATGTCTATGAAAAAGATAATTTAATTTATCATGTACTAGATAGAAAACCTTCGAGAATACATAATGTT
AAATGTTCAATAGATTGGTTAAGACGATTAGATGGAATGAATCATCATTTAGCTCAACATGTACTATCTTCATGTTTCTT
TAGACTCTTTAATATAAATACAATTGCAGTTCATCATGGAAATGAATTTAGTACCATTGACTTTAGTTCTATACTTACAG
AAGAACAGGTACGTTCTTGTGAAAAATATGCTAATAATATTATTGAAAAATCTTTAGATGTAGAATTTCTAACTCCAACT
AAAAGAGAACTTAAAAGTATGAAACTTAGAAGAGATCTTCCAGATACTAATGAACAAATACGTATTGTTAAAATTGATGA
TTTAGACCTTAATGCTTGTTGTGGTGTTCACCCTAAATCAACATTAGATTTAAGACTTATAAAAATAAAAAAATATGAAA
AACATAAAAAAGGATCTAGAATTGAATTTATAACTGGATCAAAAGCTGTAACTGATTCTTTAGAAAAAGATTTATTTGCA
AATTCTATGTGTAAATACTTAAATTGCAATGAAAAAGAAGCTTTAAATGGAATAGAAAATTTAAACTTAGAGTTAAAAAA
TGTACTGAATGAAAATAAAAGTATGAGTATTGAACTTTCAAATTATGAAATTAAAGAAATTATAGAAAAATCAGCTCAAA
TTAATAATATAAACATTATTAAATATGTATATAATAATAAAACCTTAAAATTTGTTAATACAATTGTTTCACAAATAACA
GAAAAAGATAATAAAATTGCTCTAATTGCACTAGTAAATGATGATAAAATAAACCTTATATTTTCATGTTCTAAAAATAT
AAAAAATGTTAATATGAATGAATTATTAAAAGATGCAATAGTTCTTATAGATGGACGTGGCGGTGGAAATCAAACCCTTG
CTCAAGGTGCTGGTAAGAATTTATTAAACTTAGAGAATACCTTAGACTATGCTTTTAATAAGCTTAAAAATATGTTATAA

Upstream 100 bases:

>100_bases
CTCAATATATTTATTTATTAATTAAATGATTTTTTGCAATTACTTGTTATAATGTTCTTATAGTTATATAAAATATCGTT
TAATACAAGGAGAATATATT

Downstream 100 bases:

>100_bases
AATTTATCGAATACTTAATAATTTTATTAAAAATTAAAACTTATCAAGAATAAATTGCTACTTCAGTATTTATTAATTTC
AACTAACTAAATAAGATTTG

Product: dhha1 domain protein

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MQKLYYEDQYLKKFTAEIEQVKEVGNEFHIVLNKTAFFPGGGGQRHDLGKIDEIEVLNVYEKDNLIYHVLDRKPSRIHNV
KCSIDWLRRLDGMNHHLAQHVLSSCFFRLFNINTIAVHHGNEFSTIDFSSILTEEQVRSCEKYANNIIEKSLDVEFLTPT
KRELKSMKLRRDLPDTNEQIRIVKIDDLDLNACCGVHPKSTLDLRLIKIKKYEKHKKGSRIEFITGSKAVTDSLEKDLFA
NSMCKYLNCNEKEALNGIENLNLELKNVLNENKSMSIELSNYEIKEIIEKSAQINNINIIKYVYNNKTLKFVNTIVSQIT
EKDNKIALIALVNDDKINLIFSCSKNIKNVNMNELLKDAIVLIDGRGGGNQTLAQGAGKNLLNLENTLDYAFNKLKNML

Sequences:

>Translated_399_residues
MQKLYYEDQYLKKFTAEIEQVKEVGNEFHIVLNKTAFFPGGGGQRHDLGKIDEIEVLNVYEKDNLIYHVLDRKPSRIHNV
KCSIDWLRRLDGMNHHLAQHVLSSCFFRLFNINTIAVHHGNEFSTIDFSSILTEEQVRSCEKYANNIIEKSLDVEFLTPT
KRELKSMKLRRDLPDTNEQIRIVKIDDLDLNACCGVHPKSTLDLRLIKIKKYEKHKKGSRIEFITGSKAVTDSLEKDLFA
NSMCKYLNCNEKEALNGIENLNLELKNVLNENKSMSIELSNYEIKEIIEKSAQINNINIIKYVYNNKTLKFVNTIVSQIT
EKDNKIALIALVNDDKINLIFSCSKNIKNVNMNELLKDAIVLIDGRGGGNQTLAQGAGKNLLNLENTLDYAFNKLKNML
>Mature_399_residues
MQKLYYEDQYLKKFTAEIEQVKEVGNEFHIVLNKTAFFPGGGGQRHDLGKIDEIEVLNVYEKDNLIYHVLDRKPSRIHNV
KCSIDWLRRLDGMNHHLAQHVLSSCFFRLFNINTIAVHHGNEFSTIDFSSILTEEQVRSCEKYANNIIEKSLDVEFLTPT
KRELKSMKLRRDLPDTNEQIRIVKIDDLDLNACCGVHPKSTLDLRLIKIKKYEKHKKGSRIEFITGSKAVTDSLEKDLFA
NSMCKYLNCNEKEALNGIENLNLELKNVLNENKSMSIELSNYEIKEIIEKSAQINNINIIKYVYNNKTLKFVNTIVSQIT
EKDNKIALIALVNDDKINLIFSCSKNIKNVNMNELLKDAIVLIDGRGGGNQTLAQGAGKNLLNLENTLDYAFNKLKNML

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=361, Percent_Identity=27.4238227146814, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI217416402, Length=361, Percent_Identity=27.4238227146814, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1789048, Length=388, Percent_Identity=22.4226804123711, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324288, Length=254, Percent_Identity=26.3779527559055, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI18543305, Length=253, Percent_Identity=28.4584980237154, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 45814; Mature: 45814

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLYYEDQYLKKFTAEIEQVKEVGNEFHIVLNKTAFFPGGGGQRHDLGKIDEIEVLNVY
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEE
EKDNLIYHVLDRKPSRIHNVKCSIDWLRRLDGMNHHLAQHVLSSCFFRLFNINTIAVHHG
ECCCEEEEEECCCCCHHEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEC
NEFSTIDFSSILTEEQVRSCEKYANNIIEKSLDVEFLTPTKRELKSMKLRRDLPDTNEQI
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCCE
RIVKIDDLDLNACCGVHPKSTLDLRLIKIKKYEKHKKGSRIEFITGSKAVTDSLEKDLFA
EEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHH
NSMCKYLNCNEKEALNGIENLNLELKNVLNENKSMSIELSNYEIKEIIEKSAQINNINII
HHHHHHCCCCHHHHHCCHHHCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCEEEE
KYVYNNKTLKFVNTIVSQITEKDNKIALIALVNDDKINLIFSCSKNIKNVNMNELLKDAI
EEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHCCEE
VLIDGRGGGNQTLAQGAGKNLLNLENTLDYAFNKLKNML
EEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKLYYEDQYLKKFTAEIEQVKEVGNEFHIVLNKTAFFPGGGGQRHDLGKIDEIEVLNVY
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEE
EKDNLIYHVLDRKPSRIHNVKCSIDWLRRLDGMNHHLAQHVLSSCFFRLFNINTIAVHHG
ECCCEEEEEECCCCCHHEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEC
NEFSTIDFSSILTEEQVRSCEKYANNIIEKSLDVEFLTPTKRELKSMKLRRDLPDTNEQI
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCCE
RIVKIDDLDLNACCGVHPKSTLDLRLIKIKKYEKHKKGSRIEFITGSKAVTDSLEKDLFA
EEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHH
NSMCKYLNCNEKEALNGIENLNLELKNVLNENKSMSIELSNYEIKEIIEKSAQINNINII
HHHHHHCCCCHHHHHCCHHHCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCEEEE
KYVYNNKTLKFVNTIVSQITEKDNKIALIALVNDDKINLIFSCSKNIKNVNMNELLKDAI
EEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHCCEE
VLIDGRGGGNQTLAQGAGKNLLNLENTLDYAFNKLKNML
EEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]