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The map label for this gene is pinE [H]

Identifier: 187928933

GI number: 187928933

Start: 1944948

End: 1945541

Strand: Reverse

Name: pinE [H]

Synonym: Rpic_1852

Alternate gene names: 187928933

Gene position: 1945541-1944948 (Counterclockwise)

Preceding gene: 187928938

Following gene: 187928932

Centisome position: 49.35

GC content: 66.5

Gene sequence:

>594_bases
ATGTTGGTAGGTTACATGCGCGTGTCGTCGGACTCCGACCGCCAGAGCACGAACTTGCAGCGCGATGCGCTGCTCGCCGT
CGGCGTCGATGCGCGGCATCTGTTCGAGGATCATGCTTCCGGCGCGAAGGACGACCGCGCGGGCCTGGCGCGGGCGCTCG
AATTCGTTCGCCCTGGCGACGTGTTGGTCGTGTGGAAGCTCGACCGGCTCGGCCGTTCGTTGTCGCACTTGCTCGCCATC
GTGACCTCGCTCAAGAAAAAGCAGGTGGCGTTCCGCTCGCTGACGGAGAACCTGGATACCACGACGCCCTCGGGCGAGTT
TCTGTTCCAGGTGTTCGGCGCGCTCGCGCAGTACGAACGCGCCTTGATCCAGGAACGTGTCGTCGCCGGTCTGGCTGCCG
CCCGCAAACGCGGCCGGATCGGCGGCCGGCCGCAGGCGATCACCGGCGAGAAGCTGGAGGCCATCGTCGCTGCGCTCGAT
GGCGGCATGTCCAAGGCGGCGGTGTGCCGCAACTTCGGCGTCAAGCGAACCACGCTGATCGAGACCCTGGCACGGGTTGG
TTGGACGGGCTCTCGTGGAGCGTCATCGCGATGA

Upstream 100 bases:

>100_bases
CGGTCCTCTGCACCAACCTCCGAGTGGCCGCAAAATTGTGCGGAAAACTCTGTCGCCAGACGCTACCATACGGAAACCTC
GTCTTAATGGTTTTCCGCTT

Downstream 100 bases:

>100_bases
CGACCAAGAGCGAACGATTGACCGTCCTGTCGGACGCCGAGCAGGAAGCCCTGTACGGCCTGCCGGACTTCGACGACGCC
CAGCGGCTGGAATACTTGGC

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MLVGYMRVSSDSDRQSTNLQRDALLAVGVDARHLFEDHASGAKDDRAGLARALEFVRPGDVLVVWKLDRLGRSLSHLLAI
VTSLKKKQVAFRSLTENLDTTTPSGEFLFQVFGALAQYERALIQERVVAGLAAARKRGRIGGRPQAITGEKLEAIVAALD
GGMSKAAVCRNFGVKRTTLIETLARVGWTGSRGASSR

Sequences:

>Translated_197_residues
MLVGYMRVSSDSDRQSTNLQRDALLAVGVDARHLFEDHASGAKDDRAGLARALEFVRPGDVLVVWKLDRLGRSLSHLLAI
VTSLKKKQVAFRSLTENLDTTTPSGEFLFQVFGALAQYERALIQERVVAGLAAARKRGRIGGRPQAITGEKLEAIVAALD
GGMSKAAVCRNFGVKRTTLIETLARVGWTGSRGASSR
>Mature_197_residues
MLVGYMRVSSDSDRQSTNLQRDALLAVGVDARHLFEDHASGAKDDRAGLARALEFVRPGDVLVVWKLDRLGRSLSHLLAI
VTSLKKKQVAFRSLTENLDTTTPSGEFLFQVFGALAQYERALIQERVVAGLAAARKRGRIGGRPQAITGEKLEAIVAALD
GGMSKAAVCRNFGVKRTTLIETLARVGWTGSRGASSR

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=151, Percent_Identity=52.317880794702, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1787827, Length=190, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1787638, Length=190, Percent_Identity=31.0526315789474, Blast_Score=94, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21320; Mature: 21320

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVGYMRVSSDSDRQSTNLQRDALLAVGVDARHLFEDHASGAKDDRAGLARALEFVRPGD
CEEEEEECCCCCCHHHHCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
VLVVWKLDRLGRSLSHLLAIVTSLKKKQVAFRSLTENLDTTTPSGEFLFQVFGALAQYER
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ALIQERVVAGLAAARKRGRIGGRPQAITGEKLEAIVAALDGGMSKAAVCRNFGVKRTTLI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHH
ETLARVGWTGSRGASSR
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLVGYMRVSSDSDRQSTNLQRDALLAVGVDARHLFEDHASGAKDDRAGLARALEFVRPGD
CEEEEEECCCCCCHHHHCHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
VLVVWKLDRLGRSLSHLLAIVTSLKKKQVAFRSLTENLDTTTPSGEFLFQVFGALAQYER
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ALIQERVVAGLAAARKRGRIGGRPQAITGEKLEAIVAALDGGMSKAAVCRNFGVKRTTLI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHH
ETLARVGWTGSRGASSR
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]