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The map label for this gene is azoB [H]

Identifier: 187927647

GI number: 187927647

Start: 587072

End: 587935

Strand: Reverse

Name: azoB [H]

Synonym: Rpic_0547

Alternate gene names: 187927647

Gene position: 587935-587072 (Counterclockwise)

Preceding gene: 187927649

Following gene: 187927646

Centisome position: 14.91

GC content: 65.05

Gene sequence:

>864_bases
ATGACCATCCTCGTTACTGGTGCTACCGGCCGCGTCGGCCGCCAAGTCGTCCATCAACTTGCCAACCGCGGTGCGGACGT
GCGCGCGCTGGTGCGCGACCCGTCAAAGGCCGACTTCCCGGCCAGCGTGAACGTCGTGCAGGGCGACATGCTGGATCTCG
ACTCGCTCCGGCGTGCCTTCGTTGGTGTCCGGACGCTGTTCCTGCTCAACGCGGTCGCGGGGGACGAATTCACCCAGGCG
CTCATTGCACTGAACGTTGCCCGCGAATCGGGCGTTGAGCGCGTCGTCTATCTGTCCGTCATGCATGCCGAGCGCTTCGT
GAACGTGCCGCACTTTGCGGTGAAGTCCGGCGCGGAACGCATGATCGAGCAGATGGGCTTCAGCGCCACCATCCTGCGTC
CGGCCTACTTCATGGACAACGAGCACATGGTCAAGGACGTCATCGTCAACCATGGTGTCTACCCGATGCCGATCGGCAGC
AAGGGGGTTGCGATGGTCGACACGCGCGACATCGCAGAAGTCGCGGCCATCGAGCTGATCCGCCGGGACGCGGCACCGGG
CAAGCTGCCGGTCGAGACCATCAACCTCGTGGGCCCCGATACGCTGACGGGCCCCGAACTGGCCTCGATCTGGACAGAAA
CGCTCCGCCGCCCCGTGGCCTACGGCGGCGACGACCCGACCGCATTCGAGAGCAACCTGACCAACTTCCTGCCCAAATGG
ATGGCTTATGAGATGCGCCTGATGGCCGAACGCTATGTCAGCGACGGCATGGTGCCGCAGGCGGGCGATGTCGAGCGCCT
GACCAGCATTCTGGGGCGGCCGCTGGGCACGTATCGCGATTTCGCTGCGGCGCTTGCGCAGTGA

Upstream 100 bases:

>100_bases
TTCCGCCAGTGAGATACCTCGTCTCTTTATAGCATCTTCCGGACCACAGATGGATCACCTACCTTAGCTGGGAACCCAAC
CCACTCAAGGAGTGAACATC

Downstream 100 bases:

>100_bases
GCCGCGCGCGGGGCGGGGCAGGCGACAGGCGGGGGCACTGACGCCCGCCTGCCCTCTTCCGCGCCCTTGCGCTGAGCGGG
AACCACGTCAACCGCTCGCC

Product: NmrA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTILVTGATGRVGRQVVHQLANRGADVRALVRDPSKADFPASVNVVQGDMLDLDSLRRAFVGVRTLFLLNAVAGDEFTQA
LIALNVARESGVERVVYLSVMHAERFVNVPHFAVKSGAERMIEQMGFSATILRPAYFMDNEHMVKDVIVNHGVYPMPIGS
KGVAMVDTRDIAEVAAIELIRRDAAPGKLPVETINLVGPDTLTGPELASIWTETLRRPVAYGGDDPTAFESNLTNFLPKW
MAYEMRLMAERYVSDGMVPQAGDVERLTSILGRPLGTYRDFAAALAQ

Sequences:

>Translated_287_residues
MTILVTGATGRVGRQVVHQLANRGADVRALVRDPSKADFPASVNVVQGDMLDLDSLRRAFVGVRTLFLLNAVAGDEFTQA
LIALNVARESGVERVVYLSVMHAERFVNVPHFAVKSGAERMIEQMGFSATILRPAYFMDNEHMVKDVIVNHGVYPMPIGS
KGVAMVDTRDIAEVAAIELIRRDAAPGKLPVETINLVGPDTLTGPELASIWTETLRRPVAYGGDDPTAFESNLTNFLPKW
MAYEMRLMAERYVSDGMVPQAGDVERLTSILGRPLGTYRDFAAALAQ
>Mature_286_residues
TILVTGATGRVGRQVVHQLANRGADVRALVRDPSKADFPASVNVVQGDMLDLDSLRRAFVGVRTLFLLNAVAGDEFTQAL
IALNVARESGVERVVYLSVMHAERFVNVPHFAVKSGAERMIEQMGFSATILRPAYFMDNEHMVKDVIVNHGVYPMPIGSK
GVAMVDTRDIAEVAAIELIRRDAAPGKLPVETINLVGPDTLTGPELASIWTETLRRPVAYGGDDPTAFESNLTNFLPKWM
AYEMRLMAERYVSDGMVPQAGDVERLTSILGRPLGTYRDFAAALAQ

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=284, Percent_Identity=26.4084507042254, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 31372; Mature: 31240

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILVTGATGRVGRQVVHQLANRGADVRALVRDPSKADFPASVNVVQGDMLDLDSLRRAF
CEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHH
VGVRTLFLLNAVAGDEFTQALIALNVARESGVERVVYLSVMHAERFVNVPHFAVKSGAER
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MIEQMGFSATILRPAYFMDNEHMVKDVIVNHGVYPMPIGSKGVAMVDTRDIAEVAAIELI
HHHHCCCCEEEECHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHH
RRDAAPGKLPVETINLVGPDTLTGPELASIWTETLRRPVAYGGDDPTAFESNLTNFLPKW
HHCCCCCCCCHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MAYEMRLMAERYVSDGMVPQAGDVERLTSILGRPLGTYRDFAAALAQ
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TILVTGATGRVGRQVVHQLANRGADVRALVRDPSKADFPASVNVVQGDMLDLDSLRRAF
EEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHH
VGVRTLFLLNAVAGDEFTQALIALNVARESGVERVVYLSVMHAERFVNVPHFAVKSGAER
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MIEQMGFSATILRPAYFMDNEHMVKDVIVNHGVYPMPIGSKGVAMVDTRDIAEVAAIELI
HHHHCCCCEEEECHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHH
RRDAAPGKLPVETINLVGPDTLTGPELASIWTETLRRPVAYGGDDPTAFESNLTNFLPKW
HHCCCCCCCCHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MAYEMRLMAERYVSDGMVPQAGDVERLTSILGRPLGTYRDFAAALAQ
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]