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The map label for this gene is flhA [H]

Identifier: 187927635

GI number: 187927635

Start: 570719

End: 571825

Strand: Reverse

Name: flhA [H]

Synonym: Rpic_0535

Alternate gene names: 187927635

Gene position: 571825-570719 (Counterclockwise)

Preceding gene: 187927637

Following gene: 187927634

Centisome position: 14.5

GC content: 63.23

Gene sequence:

>1107_bases
ATGAAAACCAAAGCCGCCATCGCCTGGAAAGCCGGTGCTCCGCTCACTGTGGAAGAAGTGGACCTGCAAGGCCCGCGTGC
CGGCGAGGTGCTCGTCGAGATCAAGGCGACCGGCATCTGCCATACCGACTACTACACGCTCTCGGGCGCAGACCCGGAAG
GCATCTTCCCGGCGATCCTCGGGCATGAGGGCGCAGGGGTGGTCCTTGAGGTGGGAGCAGGAGTCACATCGTTGAAAGCC
GGCGACCACGTGATCCCGCTTTACACGCCGGAATGCCGCCAGTGCAAGTTTTGCCTGTCGCGCAAGACGAACCTGTGCCA
GGCCATTCGTGCCACGCAAGGCAAGGGCCTGATGCCGGACGGCACGTCGCGCTTCTCGCTCGATGGCAAGCCGCTGTTTC
ACTACATGGGCACGTCCACGTTTGCCAATCACATCGTGGTGCCGGAGATTGCGCTGGCAAAGATCCGCCCCGACGCGCCG
TTCGACAAGGTCTGCTACATCGGCTGTGGCGTCACCACGGGCGTGGGCGCGGTGGTCTACACGGCCAAGGTGGAACCGGG
CGCCAACGTGGTCGTGTTCGGTCTGGGCGGCATCGGCTTGAACGTGATCCAGGGCGCGAAGATGGTGGGCGCAGACAAGA
TCATCGGCGTGGACCTGAACCCCGCACGCGAGGCGATGGCACGCAAATTCGGCATGACGCATTTCATCAACCCGAGCGAT
GTGGAGAACGTGGTCGATCACATCATCCAGCTCACCGATGGCGGCGCGGATTACTCGTTCGAATGCATCGGCAACACGAA
GGTCATGCGCCAGGCGCTGGAGTGCTGCCACAAGGGCTGGGGCCAATCGATCATCATCGGCGTGGCCGAAGCCGGCGCGG
AGATCAGCACGCGCCCGTTCCAGCTCGTGACCGGCCGTGAGTGGAAGGGCTCCGCGTTCGGCGGCGCCCGCGGCCGCACC
GACGTGCCCAAGATCGTCGACTGGTACATGGAAGGCAAGCTCAACATCGACGACCTCATCACGCACACGCTGCCGCTGGA
GCGCATCAACGAAGGCTTCGAGCTGATGAAGCGCGGTGAATCGATCCGTTCGGTCGTGTTGTATTGA

Upstream 100 bases:

>100_bases
ATGACGTTGTTGTCACGTTGCCGGGGCAGGCAACGTTCTACAATCGGCCTCCCTACACTGCCCACGTCGGGCAGGCTCCA
CTTCAAAAAAAGGAATGCGC

Downstream 100 bases:

>100_bases
TCCTTGGGGATGGAGCCACGCGATGACGATCCCGGTACCGGCACTTGAACTGCTTTCCGAACACGCCTGCTTTGGTGGCG
TGCAGCGCTTCTATCGGCAT

Product: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAIAWKAGAPLTVEEVDLQGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVLEVGAGVTSLKA
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSLDGKPLFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVVYTAKVEPGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFINPSD
VENVVDHIIQLTDGGADYSFECIGNTKVMRQALECCHKGWGQSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKLNIDDLITHTLPLERINEGFELMKRGESIRSVVLY

Sequences:

>Translated_368_residues
MKTKAAIAWKAGAPLTVEEVDLQGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVLEVGAGVTSLKA
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSLDGKPLFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVVYTAKVEPGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFINPSD
VENVVDHIIQLTDGGADYSFECIGNTKVMRQALECCHKGWGQSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKLNIDDLITHTLPLERINEGFELMKRGESIRSVVLY
>Mature_368_residues
MKTKAAIAWKAGAPLTVEEVDLQGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVLEVGAGVTSLKA
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSLDGKPLFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVVYTAKVEPGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFINPSD
VENVVDHIIQLTDGGADYSFECIGNTKVMRQALECCHKGWGQSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKLNIDDLITHTLPLERINEGFELMKRGESIRSVVLY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=62.125340599455, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=46.524064171123, Blast_Score=358, Evalue=3e-99,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=45.7994579945799, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.5284552845528, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=45.2574525745258, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=45.4054054054054, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=45.4054054054054, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=44.8369565217391, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=44.8753462603878, Blast_Score=335, Evalue=3e-92,
Organism=Homo sapiens, GI156627571, Length=358, Percent_Identity=25.9776536312849, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=59.349593495935, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI87082125, Length=382, Percent_Identity=26.9633507853403, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788073, Length=361, Percent_Identity=27.4238227146814, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790045, Length=376, Percent_Identity=28.1914893617021, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1786825, Length=400, Percent_Identity=26.25, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI226510992, Length=373, Percent_Identity=24.6648793565684, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787863, Length=358, Percent_Identity=26.536312849162, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081918, Length=342, Percent_Identity=29.2397660818713, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=26.4305177111717, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=54.6916890080429, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=54.4, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=54.8104956268222, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17562584, Length=367, Percent_Identity=29.700272479564, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17562582, Length=364, Percent_Identity=26.6483516483516, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71988145, Length=372, Percent_Identity=26.0752688172043, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=54.7169811320755, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6319621, Length=370, Percent_Identity=27.5675675675676, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323099, Length=372, Percent_Identity=29.0322580645161, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324486, Length=374, Percent_Identity=26.7379679144385, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=365, Percent_Identity=26.3013698630137, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=365, Percent_Identity=25.7534246575342, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322619, Length=365, Percent_Identity=28.4931506849315, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319955, Length=365, Percent_Identity=28.4931506849315, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319257, Length=362, Percent_Identity=24.8618784530387, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=60.054347826087, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17137530, Length=341, Percent_Identity=28.1524926686217, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17737897, Length=341, Percent_Identity=28.1524926686217, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39392; Mature: 39392

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAIAWKAGAPLTVEEVDLQGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEECCCCCCEEEEECCCCCCCCCEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGVVLEVGAGVTSLKAGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPD
CCCCCCEEEEECCCEEEECCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
GTSRFSLDGKPLFHYMGTSTFANHIVVPEIALAKIRPDAPFDKVCYIGCGVTTGVGAVVY
CCCEEECCCCEEEEECCCHHHHCCEECCCEEEEEECCCCCCCCEEEEECCCHHCCCEEEE
TAKVEPGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFINPSD
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCCCCCCHHH
VENVVDHIIQLTDGGADYSFECIGNTKVMRQALECCHKGWGQSIIIGVAEAGAEISTRPF
HHHHHHHHHEECCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHTLPLERINEGFELMKRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCC
SIRSVVLY
HHHHEECC
>Mature Secondary Structure
MKTKAAIAWKAGAPLTVEEVDLQGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEECCCCCCEEEEECCCCCCCCCEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGVVLEVGAGVTSLKAGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPD
CCCCCCEEEEECCCEEEECCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
GTSRFSLDGKPLFHYMGTSTFANHIVVPEIALAKIRPDAPFDKVCYIGCGVTTGVGAVVY
CCCEEECCCCEEEEECCCHHHHCCEECCCEEEEEECCCCCCCCEEEEECCCHHCCCEEEE
TAKVEPGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFINPSD
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCHHHHHHHHHHCCCCCCCHHH
VENVVDHIIQLTDGGADYSFECIGNTKVMRQALECCHKGWGQSIIIGVAEAGAEISTRPF
HHHHHHHHHEECCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHTLPLERINEGFELMKRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCC
SIRSVVLY
HHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]