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The map label for this gene is dacC [H]

Identifier: 187927600

GI number: 187927600

Start: 532507

End: 534045

Strand: Reverse

Name: dacC [H]

Synonym: Rpic_0500

Alternate gene names: 187927600

Gene position: 534045-532507 (Counterclockwise)

Preceding gene: 187927601

Following gene: 187927599

Centisome position: 13.55

GC content: 66.6

Gene sequence:

>1539_bases
ATGGCCCGAATTCGTCCGACCCGTTTGCTGACCCTGTCGCGCAGCGCGCTTGCCTGCGCCGTGCTCTTGACCATTGCGGC
CTCGCCGCTGGCGCACGCCAAGAAAGCCAAGACCCAGGCGGCACCGCGCACCACCGCCGCGCAAACACGCCAGGCTGCAC
ACAACCCTGCCGGGTTGCCCGCCAACGTGGCGCTGGCTTTTGCGCGCGCGCATATCCCGCTGGATGCGGTGAGCGTGTTC
GTGATCCGCACCGGCACCGCCACGCCCATCCTGCAATGGAATGCCGACGCCGGAATGAACCCGGCGTCGACCATGAAGCT
GCTGACCACCTTCGCGGGGCTGGACCTGCTTGGCCCGGATTTTCGCTGGAAGACCACGGCCTACGCCGACAGCGCTCCGG
TCAACGGCACGCTCAACGGCAACCTGTACCTACGTGGCCAGGGCGATCCGAAGCTGATTCCCGAAGAGCTCGTCAAGCTG
GTGACCGACGTGCGCCGCGCCGGCGTGGATGAACTGGCGGGCAACATCGTGCTGGATCGCACCTACTTCGAGGGCGATCT
GTCGGAAGCACCGCCGCTGGACGGCGACAGCGCGCGTGCCTACAACGTCGCGCCCGACGCGCTGCTGTATTCATTCAAGA
CGCTCACGTTCACGCTCTCGCCGGATGCCGGCAACGGCGCGGTCAACATCGATGTATCACCGCCGCTGGCGCAGTTGCAG
ATCGATAACCAGTTGCGCACCACGCGCGGCGGCTGCGGCGACTGGAAGACGACCTCCGGCGCCAACATCTCCACGCAGAC
CGACGGCCACGTCATAGCCAGCTTCGACGGCCGCTATGCCGCCGCGTGCGGCGAACGCGTCTTCAATATCGCCGCGCTCA
CGCATGCCGACTTCATCTGGGGTGGCTTCCTGGCGCTATGGCAGCAGGCCGGTGGCACGACGCGTTTCACGCCGGGCTTG
CGCGAAGGCAAGGTACCGCGTCAGGCCGTTCTGCTGGCCACGCACTACGGCCCGCCGCTGTCGGAAGTCGTGCACGACAT
CGACAAATATTCGAACAACGTGATGGCGCGCCAGCTCTTCCTCACCATCGGCGCGGAAATCGGGCGCAAGCCCGCATCGG
TGCGGCAATCGACCGAGGTCATCAACCGCTGGCTCGCCAAACAGAACCTCACGATGCCGGAGCTGGTGCTGGAGAACGGT
TCGGGCCTGTCACGCACCGAGCGCATCAGCGCACGCAACATGGGGCGCCTGCTGCAGCAGGCCGACGCCAACCCGAACGG
CGGCGTGCTGCGCGATGCGCTACCCGTTGTCGGCGTGGACGGCACCATGCGCAATCGCCTGACACGGGCCGGGGTGGCCG
GCAATGCCGAGATCAAGACCGGCACACTCAACGGCGTGCGCGCCATCGCCGGGTATGTGGAAGGCGAAGACGGCCAGCGC
TTCGTGGTGGTCAGCATGATCAACCACCCGAACGCCAGCGGCGGCCAAGCCGCGCACGATGCACTGCTGCAATGGATCTA
CCAGGGCGCGCCGCGATGA

Upstream 100 bases:

>100_bases
ACGCCCCATTGCTTGTCGGCCGAAGTGCGGGGCCACTGCGTTAACATGCCTGCGTCTGCACTTGGCGCATGTGTGCGCGC
CCCACTCTGCCCCCCCGCCG

Downstream 100 bases:

>100_bases
GCTCACGCAAGTCACTGCTGGGCATCACCGGCACGTCGGGCAGCGGCAAGACCACGCTCGTCGAGCAACTCATCGCCCGT
TTCGTGCACGACGGGCGCCG

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 4a; PBP-4a [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MARIRPTRLLTLSRSALACAVLLTIAASPLAHAKKAKTQAAPRTTAAQTRQAAHNPAGLPANVALAFARAHIPLDAVSVF
VIRTGTATPILQWNADAGMNPASTMKLLTTFAGLDLLGPDFRWKTTAYADSAPVNGTLNGNLYLRGQGDPKLIPEELVKL
VTDVRRAGVDELAGNIVLDRTYFEGDLSEAPPLDGDSARAYNVAPDALLYSFKTLTFTLSPDAGNGAVNIDVSPPLAQLQ
IDNQLRTTRGGCGDWKTTSGANISTQTDGHVIASFDGRYAAACGERVFNIAALTHADFIWGGFLALWQQAGGTTRFTPGL
REGKVPRQAVLLATHYGPPLSEVVHDIDKYSNNVMARQLFLTIGAEIGRKPASVRQSTEVINRWLAKQNLTMPELVLENG
SGLSRTERISARNMGRLLQQADANPNGGVLRDALPVVGVDGTMRNRLTRAGVAGNAEIKTGTLNGVRAIAGYVEGEDGQR
FVVVSMINHPNASGGQAAHDALLQWIYQGAPR

Sequences:

>Translated_512_residues
MARIRPTRLLTLSRSALACAVLLTIAASPLAHAKKAKTQAAPRTTAAQTRQAAHNPAGLPANVALAFARAHIPLDAVSVF
VIRTGTATPILQWNADAGMNPASTMKLLTTFAGLDLLGPDFRWKTTAYADSAPVNGTLNGNLYLRGQGDPKLIPEELVKL
VTDVRRAGVDELAGNIVLDRTYFEGDLSEAPPLDGDSARAYNVAPDALLYSFKTLTFTLSPDAGNGAVNIDVSPPLAQLQ
IDNQLRTTRGGCGDWKTTSGANISTQTDGHVIASFDGRYAAACGERVFNIAALTHADFIWGGFLALWQQAGGTTRFTPGL
REGKVPRQAVLLATHYGPPLSEVVHDIDKYSNNVMARQLFLTIGAEIGRKPASVRQSTEVINRWLAKQNLTMPELVLENG
SGLSRTERISARNMGRLLQQADANPNGGVLRDALPVVGVDGTMRNRLTRAGVAGNAEIKTGTLNGVRAIAGYVEGEDGQR
FVVVSMINHPNASGGQAAHDALLQWIYQGAPR
>Mature_511_residues
ARIRPTRLLTLSRSALACAVLLTIAASPLAHAKKAKTQAAPRTTAAQTRQAAHNPAGLPANVALAFARAHIPLDAVSVFV
IRTGTATPILQWNADAGMNPASTMKLLTTFAGLDLLGPDFRWKTTAYADSAPVNGTLNGNLYLRGQGDPKLIPEELVKLV
TDVRRAGVDELAGNIVLDRTYFEGDLSEAPPLDGDSARAYNVAPDALLYSFKTLTFTLSPDAGNGAVNIDVSPPLAQLQI
DNQLRTTRGGCGDWKTTSGANISTQTDGHVIASFDGRYAAACGERVFNIAALTHADFIWGGFLALWQQAGGTTRFTPGLR
EGKVPRQAVLLATHYGPPLSEVVHDIDKYSNNVMARQLFLTIGAEIGRKPASVRQSTEVINRWLAKQNLTMPELVLENGS
GLSRTERISARNMGRLLQQADANPNGGVLRDALPVVGVDGTMRNRLTRAGVAGNAEIKTGTLNGVRAIAGYVEGEDGQRF
VVVSMINHPNASGGQAAHDALLQWIYQGAPR

Specific function: Catalyzes DD-carboxypeptidase and transpeptidation reactions [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted (Probable). Note=May be anchored in the membrane via a C-terminal amphiphatic alpha helix [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=431, Percent_Identity=25.754060324826, Blast_Score=129, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 54534; Mature: 54402

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIRPTRLLTLSRSALACAVLLTIAASPLAHAKKAKTQAAPRTTAAQTRQAAHNPAGLP
CCCCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
ANVALAFARAHIPLDAVSVFVIRTGTATPILQWNADAGMNPASTMKLLTTFAGLDLLGPD
HHHHHHHHHHCCCHHHEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHCCCC
FRWKTTAYADSAPVNGTLNGNLYLRGQGDPKLIPEELVKLVTDVRRAGVDELAGNIVLDR
CCEEEEECCCCCCCCEEECCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHCCCEEEEE
TYFEGDLSEAPPLDGDSARAYNVAPDALLYSFKTLTFTLSPDAGNGAVNIDVSPPLAQLQ
EEECCCCCCCCCCCCCCCCEECCCHHHHHEEEEEEEEEECCCCCCCEEEEECCCCCEEEE
IDNQLRTTRGGCGDWKTTSGANISTQTDGHVIASFDGRYAAACGERVFNIAALTHADFIW
ECCHHHHCCCCCCCCCCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
GGFLALWQQAGGTTRFTPGLREGKVPRQAVLLATHYGPPLSEVVHDIDKYSNNVMARQLF
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHH
LTIGAEIGRKPASVRQSTEVINRWLAKQNLTMPELVLENGSGLSRTERISARNMGRLLQQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ADANPNGGVLRDALPVVGVDGTMRNRLTRAGVAGNAEIKTGTLNGVRAIAGYVEGEDGQR
HCCCCCCCCHHHCCCEEECCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHEEECCCCCE
FVVVSMINHPNASGGQAAHDALLQWIYQGAPR
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARIRPTRLLTLSRSALACAVLLTIAASPLAHAKKAKTQAAPRTTAAQTRQAAHNPAGLP
CCCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
ANVALAFARAHIPLDAVSVFVIRTGTATPILQWNADAGMNPASTMKLLTTFAGLDLLGPD
HHHHHHHHHHCCCHHHEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHCCCC
FRWKTTAYADSAPVNGTLNGNLYLRGQGDPKLIPEELVKLVTDVRRAGVDELAGNIVLDR
CCEEEEECCCCCCCCEEECCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHCCCEEEEE
TYFEGDLSEAPPLDGDSARAYNVAPDALLYSFKTLTFTLSPDAGNGAVNIDVSPPLAQLQ
EEECCCCCCCCCCCCCCCCEECCCHHHHHEEEEEEEEEECCCCCCCEEEEECCCCCEEEE
IDNQLRTTRGGCGDWKTTSGANISTQTDGHVIASFDGRYAAACGERVFNIAALTHADFIW
ECCHHHHCCCCCCCCCCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
GGFLALWQQAGGTTRFTPGLREGKVPRQAVLLATHYGPPLSEVVHDIDKYSNNVMARQLF
HHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHH
LTIGAEIGRKPASVRQSTEVINRWLAKQNLTMPELVLENGSGLSRTERISARNMGRLLQQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ADANPNGGVLRDALPVVGVDGTMRNRLTRAGVAGNAEIKTGTLNGVRAIAGYVEGEDGQR
HCCCCCCCCHHHCCCEEECCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHEEECCCCCE
FVVVSMINHPNASGGQAAHDALLQWIYQGAPR
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7711903; 9384377 [H]