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The map label for this gene is miaB [H]

Identifier: 187927511

GI number: 187927511

Start: 434743

End: 436113

Strand: Reverse

Name: miaB [H]

Synonym: Rpic_0408

Alternate gene names: 187927511

Gene position: 436113-434743 (Counterclockwise)

Preceding gene: 187927512

Following gene: 187927510

Centisome position: 11.06

GC content: 63.97

Gene sequence:

>1371_bases
ATGAAAAAAGTCTTCATCAAAACCTACGGCTGCCAGATGAACGAGTACGACTCGGACAAGATGTCCGACGTGCTCAACGC
GGCTGAAGGTCTCGTCCCCACCGACACGCCCGAAGATGCGGACGTGATCCTCTTCAACACATGCTCCGTACGCGAGAAGG
CGCAGGAGAAAGTGTTCTCCGAACTCGGCCGCGTGAAGGCGCTCAAGGCGCTCAAACCGGATCTCGTGGTCGGCGTAGGT
GGCTGCGTGGCCAGCCAGGAAGGTGCGTCCATCGTGGCGCGCGCCCCTTACGTTGACGTGGTGTTCGGGCCGCAGACGCT
GCACCGCCTGCCCGACCTGATCGCAGCACGCCGCCGCACGGGCCGCTCGCAGGTCGACGTGTCCTTCCCCGAGATCGAGA
AGTTCGACCACCTGCCGCCCGCGCGCGTGGATGGCGCCAGCGCCTACGTGTCAATCATGGAAGGCTGCTCGAAATACTGC
AGCTACTGCGTGGTGCCGTACACGCGCGGCGAGGAAGTCTCGCGCCCGTTCGACGACGTGCTGGCCGAAGTGGCCGGCCT
GGCCGAACAGGGCGTGCGCGAAGTCACGTTGCTGGGCCAGAACGTCAACGCCTACATCGGCAAGATGGGCGACACGAGCG
AGCGTGCGGATTTCGCGCTGCTGCTCGAATACGTGGCTGAGATTCCGGGTATCGAGCGCATCCGCTACACGACCAGCCAC
CCGAAGGAATTCAGCTCGCGCCTGATCGAGGCCTATGCCACCAACCCGAAGCTCGTCGACCACCTGCACCTGCCCGTGCA
GCACGGCTCCGACCGCATCCTGATGGCGATGAAGCGCGGCTACACGGTGCTCGAATACAAGAGCAGCATCCGCAAGCTGC
GCGCGATCCGGCCGAACATCTCGATCGCCACCGACTTCATCGTCGGCTTTCCGGGCGAGACCGATGCAGACTTCGCCAAG
ACGATGGACCTGATTCACGAGATCGGCTACGACACCTCGTTCAGCTTCATCTACAGCCCGCGCCCGGGCACGCCGGCCGC
GAACCTGCACGACGATACGCCCCAGGCCGTCAAGCTCGAACGCCTAAAACACTTGCAGGCGACCATTGAAGAGAACGTGG
CGCGCATCAGCCAAGGCATGGTCGGCAGCGTGCAGCGCATTCTGGTGGAAGGCCCGTCGCGCAAGGATCCGACCGAACTG
CACGGCCGCACCGAGAACAACCGCGTCGTCAACTTCGCGCTACCCGATCTGCCGCAGGTGCGCCGCGAACAGCTGATCGG
CCAGATGCTGGACGTGCGCATCGTGCACGCGTTCCCGCATTCGCTGCGTGGCGAAGTGGCCGAAGAGCGTATGGCCAGCG
CGGCTCACTGA

Upstream 100 bases:

>100_bases
AAGGCTGCGGTGGCGGCACAAAGTCAGGCGCAAGCGCAGTCCCAGTAGTCAGAACGCCCCACGAAGCACGTGCCCCCAGG
TCAACCACTGTGAACGGCAC

Downstream 100 bases:

>100_bases
TCATCAACACGACCTGACTTGATGAAACCCACCATCGCAGAATTCACAGCGCCCAAGAACGACAACACGCGCCTGCAGAA
CCTGTGCGGGCCGCTGGACG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKKVFIKTYGCQMNEYDSDKMSDVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFSELGRVKALKALKPDLVVGVG
GCVASQEGASIVARAPYVDVVFGPQTLHRLPDLIAARRRTGRSQVDVSFPEIEKFDHLPPARVDGASAYVSIMEGCSKYC
SYCVVPYTRGEEVSRPFDDVLAEVAGLAEQGVREVTLLGQNVNAYIGKMGDTSERADFALLLEYVAEIPGIERIRYTTSH
PKEFSSRLIEAYATNPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSSIRKLRAIRPNISIATDFIVGFPGETDADFAK
TMDLIHEIGYDTSFSFIYSPRPGTPAANLHDDTPQAVKLERLKHLQATIEENVARISQGMVGSVQRILVEGPSRKDPTEL
HGRTENNRVVNFALPDLPQVRREQLIGQMLDVRIVHAFPHSLRGEVAEERMASAAH

Sequences:

>Translated_456_residues
MKKVFIKTYGCQMNEYDSDKMSDVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFSELGRVKALKALKPDLVVGVG
GCVASQEGASIVARAPYVDVVFGPQTLHRLPDLIAARRRTGRSQVDVSFPEIEKFDHLPPARVDGASAYVSIMEGCSKYC
SYCVVPYTRGEEVSRPFDDVLAEVAGLAEQGVREVTLLGQNVNAYIGKMGDTSERADFALLLEYVAEIPGIERIRYTTSH
PKEFSSRLIEAYATNPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSSIRKLRAIRPNISIATDFIVGFPGETDADFAK
TMDLIHEIGYDTSFSFIYSPRPGTPAANLHDDTPQAVKLERLKHLQATIEENVARISQGMVGSVQRILVEGPSRKDPTEL
HGRTENNRVVNFALPDLPQVRREQLIGQMLDVRIVHAFPHSLRGEVAEERMASAAH
>Mature_456_residues
MKKVFIKTYGCQMNEYDSDKMSDVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFSELGRVKALKALKPDLVVGVG
GCVASQEGASIVARAPYVDVVFGPQTLHRLPDLIAARRRTGRSQVDVSFPEIEKFDHLPPARVDGASAYVSIMEGCSKYC
SYCVVPYTRGEEVSRPFDDVLAEVAGLAEQGVREVTLLGQNVNAYIGKMGDTSERADFALLLEYVAEIPGIERIRYTTSH
PKEFSSRLIEAYATNPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSSIRKLRAIRPNISIATDFIVGFPGETDADFAK
TMDLIHEIGYDTSFSFIYSPRPGTPAANLHDDTPQAVKLERLKHLQATIEENVARISQGMVGSVQRILVEGPSRKDPTEL
HGRTENNRVVNFALPDLPQVRREQLIGQMLDVRIVHAFPHSLRGEVAEERMASAAH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=36.8972746331237, Blast_Score=305, Evalue=7e-83,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=36.8972746331237, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI93277076, Length=465, Percent_Identity=28.3870967741935, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=61.4349775784753, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1787057, Length=441, Percent_Identity=28.3446712018141, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=424, Percent_Identity=37.2641509433962, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI71996771, Length=301, Percent_Identity=30.8970099667774, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=34.5911949685535, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI19922432, Length=465, Percent_Identity=29.6774193548387, Blast_Score=154, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50523; Mature: 50523

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVFIKTYGCQMNEYDSDKMSDVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFS
CCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
ELGRVKALKALKPDLVVGVGGCVASQEGASIVARAPYVDVVFGPQTLHRLPDLIAARRRT
HHHHHHHHHHCCCCCEEECCCHHCCCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHHC
GRSQVDVSFPEIEKFDHLPPARVDGASAYVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDV
CCCCEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHCCCHHHH
LAEVAGLAEQGVREVTLLGQNVNAYIGKMGDTSERADFALLLEYVAEIPGIERIRYTTSH
HHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCC
PKEFSSRLIEAYATNPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSSIRKLRAIRPNI
HHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCCCC
SIATDFIVGFPGETDADFAKTMDLIHEIGYDTSFSFIYSPRPGTPAANLHDDTPQAVKLE
EEEEHEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
RLKHLQATIEENVARISQGMVGSVQRILVEGPSRKDPTELHGRTENNRVVNFALPDLPQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEECCCCHHH
RREQLIGQMLDVRIVHAFPHSLRGEVAEERMASAAH
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKVFIKTYGCQMNEYDSDKMSDVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFS
CCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
ELGRVKALKALKPDLVVGVGGCVASQEGASIVARAPYVDVVFGPQTLHRLPDLIAARRRT
HHHHHHHHHHCCCCCEEECCCHHCCCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHHC
GRSQVDVSFPEIEKFDHLPPARVDGASAYVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDV
CCCCEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHCCCHHHH
LAEVAGLAEQGVREVTLLGQNVNAYIGKMGDTSERADFALLLEYVAEIPGIERIRYTTSH
HHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCC
PKEFSSRLIEAYATNPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSSIRKLRAIRPNI
HHHHHHHHHHHHCCCCCHHHHEECCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCCCC
SIATDFIVGFPGETDADFAKTMDLIHEIGYDTSFSFIYSPRPGTPAANLHDDTPQAVKLE
EEEEHEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
RLKHLQATIEENVARISQGMVGSVQRILVEGPSRKDPTELHGRTENNRVVNFALPDLPQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEEECCCCHHH
RREQLIGQMLDVRIVHAFPHSLRGEVAEERMASAAH
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]