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The map label for this gene is gloA [H]

Identifier: 187927499

GI number: 187927499

Start: 421911

End: 422318

Strand: Reverse

Name: gloA [H]

Synonym: Rpic_0396

Alternate gene names: 187927499

Gene position: 422318-421911 (Counterclockwise)

Preceding gene: 187927500

Following gene: 187927498

Centisome position: 10.71

GC content: 61.76

Gene sequence:

>408_bases
ATGCGAATGCTCCACACCATGCTGCGCGTCGGCGACATGCAGCGCTCGATCGACTTCTACACCAAGGTGCTCGGCATGCA
ACTGCTGCGCACCAGCGACAACCCCGAGTACAAGTACTCGCTGGCGTTCGTCGGCTACGGCCCGGAAGCCAGCAACACGG
TCATCGAACTGACCTACAACTACGGGGTGAGCGAGTACGAACTCGGCACGGCATTCGGGCACTTGGCCATCGAGGTCGAC
GATGCCGCACAAGCCTGTGACCAGATTCGTAACGCAGGCGGCAAGGTCACGCGTGAAGCGGGCCCGGTCAAGGGCGGCAG
CACCATCATCGCGTTTGTGGAAGATCCGGATGGCTACAAGATCGAGCTGATCCAGGCGCGCTCGATGCCCGATGGCAACC
GCCACTGA

Upstream 100 bases:

>100_bases
TGCGGCGGATGCGGCGGTTGCAGAGAACGCCTGTCCTGCACGCTCGGCCACACCCTCCTACAATATTGCCTTCCCCACAG
TCCTCCAGGAAGGAAACCCC

Downstream 100 bases:

>100_bases
CGCCATCCGCCGGCCGCTGGACGGCACTGCCATCGGCCTGATGGTGGTGCTGTGCATGTGCTGGGGCTTTCAGCAGGTTG
CCATCAAGGTGGCCGCGCAC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MRMLHTMLRVGDMQRSIDFYTKVLGMQLLRTSDNPEYKYSLAFVGYGPEASNTVIELTYNYGVSEYELGTAFGHLAIEVD
DAAQACDQIRNAGGKVTREAGPVKGGSTIIAFVEDPDGYKIELIQARSMPDGNRH

Sequences:

>Translated_135_residues
MRMLHTMLRVGDMQRSIDFYTKVLGMQLLRTSDNPEYKYSLAFVGYGPEASNTVIELTYNYGVSEYELGTAFGHLAIEVD
DAAQACDQIRNAGGKVTREAGPVKGGSTIIAFVEDPDGYKIELIQARSMPDGNRH
>Mature_135_residues
MRMLHTMLRVGDMQRSIDFYTKVLGMQLLRTSDNPEYKYSLAFVGYGPEASNTVIELTYNYGVSEYELGTAFGHLAIEVD
DAAQACDQIRNAGGKVTREAGPVKGGSTIIAFVEDPDGYKIELIQARSMPDGNRH

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=151, Percent_Identity=33.7748344370861, Blast_Score=89, Evalue=8e-19,
Organism=Homo sapiens, GI217330598, Length=143, Percent_Identity=35.6643356643357, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=76.5151515151515, Blast_Score=221, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17552228, Length=140, Percent_Identity=30, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=38.1294964028777, Blast_Score=95, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20129743, Length=149, Percent_Identity=36.241610738255, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19920404, Length=142, Percent_Identity=35.2112676056338, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14927; Mature: 14927

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLHTMLRVGDMQRSIDFYTKVLGMQLLRTSDNPEYKYSLAFVGYGPEASNTVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEEEEEEEC
YGVSEYELGTAFGHLAIEVDDAAQACDQIRNAGGKVTREAGPVKGGSTIIAFVEDPDGYK
CCCCEEECCCEEEEEEEEECHHHHHHHHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCE
IELIQARSMPDGNRH
EEEEEECCCCCCCCC
>Mature Secondary Structure
MRMLHTMLRVGDMQRSIDFYTKVLGMQLLRTSDNPEYKYSLAFVGYGPEASNTVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEEEEEEEC
YGVSEYELGTAFGHLAIEVDDAAQACDQIRNAGGKVTREAGPVKGGSTIIAFVEDPDGYK
CCCCEEECCCEEEEEEEEECHHHHHHHHHHCCCCCEECCCCCCCCCCEEEEEEECCCCCE
IELIQARSMPDGNRH
EEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]