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The map label for this gene is aspS [H]

Identifier: 187927444

GI number: 187927444

Start: 361512

End: 363383

Strand: Direct

Name: aspS [H]

Synonym: Rpic_0341

Alternate gene names: 187927444

Gene position: 361512-363383 (Clockwise)

Preceding gene: 187927443

Following gene: 187927445

Centisome position: 9.17

GC content: 63.57

Gene sequence:

>1872_bases
ATGCGCGACGCTTCATTGGCGGCGCGCTTCGCGGAAAACCACAACATGCAAATGCGTACTCAATACTGCGGTCAGGTCAC
CGAACAACTGCTCGGCCAGAGCGTCACGCTGTCGGGCTGGGCCCATCGTCGTCGCGACCACGGTGGCGTGATCTTCATCG
ACCTGCGCGACCGTGAAGGCCTGGTGCAGGTCGTCTGCGACCCGGACCGCCCGGAGATGTTCAAGGTGGCCGAAGGTGTG
CGCAACGAGTTCTGCCTGCAGGTGAAGGGCGTCGTGCGCGCCCGCCCGGAAGGCACCACCAACGCCAACCTGACCAGCGG
CAAAGTTGAAGTGCTGTGCCACGAGTTGACGGTGCTCAACGCCTCCGTCACGCCGCCGTTCCAGCTGGACGACGACAACC
TGTCGGAGACCACGCGCCTGACGCACCGCGTGCTGGACCTGCGCCGTCCGCAGATGCAATACAACCTGCGCCTGCGCTAC
AAGGTCGCGATGGAAGTGCGCAAGTACCTCGACGACAAGGGCTTCATCGACATCGAAACGCCGATGCTGACCAAGAGCAC
GCCCGAAGGTGCGCGCGACTATCTGGTGCCGTCGCGCGTGAACGCCGGCCAGTTCTTCGCGCTGCCGCAGTCGCCGCAGC
TCTTCAAGCAGATGCTGATGGTGTCGGGCTTTGATCGCTACTACCAGATCACCAAGTGCTTCCGCGACGAAGACCTGCGC
GCCGACCGCCAGCCCGAATTCACCCAGATCGACTGCGAAACGTCGTTCCTGAGCGAGCAGGAGATTCGCGACCTGTTTGA
AGAGATGATCCGCACGGTGTTCAAGAACACCATGTCGGTGGACCTGGATGCCAAGTTCCCGGTGATGGAGTTCCGCGAGG
CGATGGCCCGCTTCGGTTCGGACAAGCCGGACCTGCGCGTCAAGCTCGAGTTCACCGAGCTCACCGACGCCATGAAGGAC
GTCGACTTCAAGGTGTTCTCCGGCCCGGCCAACGCCGAAGGCGGCCGCGTCGTGGCACTGCGCGTGCCGGGCGGTGCTTC
CCTGTCGCGTGGCGATATCGACGCCTACACCAAGTTCGTCGAAATCTATGGCGCCAAGGGCTTGGCCTGGATCAAGGTCA
ACGAAGTGGCCAAGGGCCGTGACGGCCTGCAATCGCCGATCGTGAAGAACCTGCACGACGCGGCCATCGCCGAGATCCTC
AAGCGCAGCGGCGCACAGGATGGCGACATCCTGTTCTTCGGCGCCGACCGCGCCAAGGTGGTCAACGACGCGATGGGTGC
GCTGCGCCTGAAGGTCGGCCATTCGGACTTCGCCAAGAGCACCGGCCTGTTCGAAGACGCCTGGAAGCCGCTGTGGGTGA
TCGACTTCCCGATGTTCGAGTACGACGAGGAAGACGCGCGCTGGGTGGCCATGCACCACCCGTTCACGAGCCCGAAGGAC
GAGCACCTGGAATACCTGGAAACCGATCCGGGCAAGTGCATCGCCAAGGCTTACGACATGGTGCTCAACGGCTGGGAAAT
CGGTGGCGGCTCGGTGCGGATCTACCGCGAAGAAGTGCAGAGCAAGGTGTTCCGCGCGTTGAAAATCAACGATGAGGAAG
CGCGTGCAAAGTTCGGCTTCCTGCTCGATGCACTGCAGTACGGCGCGCCCCCGCACGGCGGTATCGCGTTCGGTCTGGAT
CGCGTCGTCACGATGATGGCCGGCGCCGATTCGATCCGTGACGTGATCGCCTTCCCGAAGACACAGCGTGCGCAGGACTT
GCTCACGCAGGCGCCGAGCCCGGTCGACGAAAAGCAACTGCGCGAACTGCATATTCGCCTGCGCGCCGCCGAGGCCAAGG
TGGCTGCCCCGACGGCTGCAACGACCGCCTAA

Upstream 100 bases:

>100_bases
GAATCCGACCGCGCTGCCTGCAGAGGGCGGCGCCATCGCGGCACCCTGAGCCGGCGACACGAACGAAGTCGAATCGACGC
CGGCCCCCCGATCAACTTTC

Downstream 100 bases:

>100_bases
GCACCCGCGCCAACGCTCCACAACAAACCGCGCCGCCTGACAAGGGCGGCGCGGTTTGTTTTTGGGCGCCTGGGGGCAAC
TTGCAGCGCTCGGGAACATT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MRDASLAARFAENHNMQMRTQYCGQVTEQLLGQSVTLSGWAHRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKVAEGV
RNEFCLQVKGVVRARPEGTTNANLTSGKVEVLCHELTVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRY
KVAMEVRKYLDDKGFIDIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQMLMVSGFDRYYQITKCFRDEDLR
ADRQPEFTQIDCETSFLSEQEIRDLFEEMIRTVFKNTMSVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDAMKD
VDFKVFSGPANAEGGRVVALRVPGGASLSRGDIDAYTKFVEIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEIL
KRSGAQDGDILFFGADRAKVVNDAMGALRLKVGHSDFAKSTGLFEDAWKPLWVIDFPMFEYDEEDARWVAMHHPFTSPKD
EHLEYLETDPGKCIAKAYDMVLNGWEIGGGSVRIYREEVQSKVFRALKINDEEARAKFGFLLDALQYGAPPHGGIAFGLD
RVVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRAAEAKVAAPTAATTA

Sequences:

>Translated_623_residues
MRDASLAARFAENHNMQMRTQYCGQVTEQLLGQSVTLSGWAHRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKVAEGV
RNEFCLQVKGVVRARPEGTTNANLTSGKVEVLCHELTVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRY
KVAMEVRKYLDDKGFIDIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQMLMVSGFDRYYQITKCFRDEDLR
ADRQPEFTQIDCETSFLSEQEIRDLFEEMIRTVFKNTMSVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDAMKD
VDFKVFSGPANAEGGRVVALRVPGGASLSRGDIDAYTKFVEIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEIL
KRSGAQDGDILFFGADRAKVVNDAMGALRLKVGHSDFAKSTGLFEDAWKPLWVIDFPMFEYDEEDARWVAMHHPFTSPKD
EHLEYLETDPGKCIAKAYDMVLNGWEIGGGSVRIYREEVQSKVFRALKINDEEARAKFGFLLDALQYGAPPHGGIAFGLD
RVVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRAAEAKVAAPTAATTA
>Mature_623_residues
MRDASLAARFAENHNMQMRTQYCGQVTEQLLGQSVTLSGWAHRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKVAEGV
RNEFCLQVKGVVRARPEGTTNANLTSGKVEVLCHELTVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRY
KVAMEVRKYLDDKGFIDIETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQMLMVSGFDRYYQITKCFRDEDLR
ADRQPEFTQIDCETSFLSEQEIRDLFEEMIRTVFKNTMSVDLDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDAMKD
VDFKVFSGPANAEGGRVVALRVPGGASLSRGDIDAYTKFVEIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEIL
KRSGAQDGDILFFGADRAKVVNDAMGALRLKVGHSDFAKSTGLFEDAWKPLWVIDFPMFEYDEEDARWVAMHHPFTSPKD
EHLEYLETDPGKCIAKAYDMVLNGWEIGGGSVRIYREEVQSKVFRALKINDEEARAKFGFLLDALQYGAPPHGGIAFGLD
RVVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRAAEAKVAAPTAATTA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=613, Percent_Identity=38.6623164763458, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI45439306, Length=292, Percent_Identity=28.0821917808219, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI194272210, Length=258, Percent_Identity=23.6434108527132, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI5031815, Length=258, Percent_Identity=24.4186046511628, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=59.3908629441624, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=298, Percent_Identity=24.496644295302, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790571, Length=298, Percent_Identity=24.496644295302, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=621, Percent_Identity=32.3671497584541, Blast_Score=318, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17551876, Length=268, Percent_Identity=26.865671641791, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=282, Percent_Identity=23.4042553191489, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=282, Percent_Identity=23.4042553191489, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=282, Percent_Identity=23.4042553191489, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=635, Percent_Identity=34.0157480314961, Blast_Score=290, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6323011, Length=290, Percent_Identity=27.9310344827586, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324256, Length=183, Percent_Identity=28.4153005464481, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=264, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321807, Length=248, Percent_Identity=27.4193548387097, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24584738, Length=614, Percent_Identity=36.8078175895765, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=282, Percent_Identity=27.3049645390071, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24640849, Length=254, Percent_Identity=25.5905511811024, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24640851, Length=254, Percent_Identity=25.5905511811024, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 70140; Mature: 70140

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDASLAARFAENHNMQMRTQYCGQVTEQLLGQSVTLSGWAHRRRDHGGVIFIDLRDREG
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCEEEEEECCCCC
LVQVVCDPDRPEMFKVAEGVRNEFCLQVKGVVRARPEGTTNANLTSGKVEVLCHELTVLN
EEEEEECCCCCHHHHHHHHHHHHHEEEEEHHHEECCCCCCCCCCCCCHHHEEEEEHEEEE
ASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDDKGFIDIET
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHCCCCCEEEECC
PMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQMLMVSGFDRYYQITKCFRDEDLR
CCCCCCCCCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
ADRQPEFTQIDCETSFLSEQEIRDLFEEMIRTVFKNTMSVDLDAKFPVMEFREAMARFGS
CCCCCCCEEEECCHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCC
DKPDLRVKLEFTELTDAMKDVDFKVFSGPANAEGGRVVALRVPGGASLSRGDIDAYTKFV
CCCCEEEEEEHHHHHHHHHHCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
EIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEILKRSGAQDGDILFFGADRAKV
HHHCCCCEEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHH
VNDAMGALRLKVGHSDFAKSTGLFEDAWKPLWVIDFPMFEYDEEDARWVAMHHPFTSPKD
HHHHHHHEEEEECCCHHHHHCCCHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCH
EHLEYLETDPGKCIAKAYDMVLNGWEIGGGSVRIYREEVQSKVFRALKINDEEARAKFGF
HHHHHHCCCCCHHHHHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHH
LLDALQYGAPPHGGIAFGLDRVVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDEKQL
HHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHH
RELHIRLRAAEAKVAAPTAATTA
HHHHHHHEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRDASLAARFAENHNMQMRTQYCGQVTEQLLGQSVTLSGWAHRRRDHGGVIFIDLRDREG
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCEEEEEECCCCC
LVQVVCDPDRPEMFKVAEGVRNEFCLQVKGVVRARPEGTTNANLTSGKVEVLCHELTVLN
EEEEEECCCCCHHHHHHHHHHHHHEEEEEHHHEECCCCCCCCCCCCCHHHEEEEEHEEEE
ASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDDKGFIDIET
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHCCCCCEEEECC
PMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQMLMVSGFDRYYQITKCFRDEDLR
CCCCCCCCCCCHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
ADRQPEFTQIDCETSFLSEQEIRDLFEEMIRTVFKNTMSVDLDAKFPVMEFREAMARFGS
CCCCCCCEEEECCHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCC
DKPDLRVKLEFTELTDAMKDVDFKVFSGPANAEGGRVVALRVPGGASLSRGDIDAYTKFV
CCCCEEEEEEHHHHHHHHHHCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
EIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEILKRSGAQDGDILFFGADRAKV
HHHCCCCEEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHH
VNDAMGALRLKVGHSDFAKSTGLFEDAWKPLWVIDFPMFEYDEEDARWVAMHHPFTSPKD
HHHHHHHEEEEECCCHHHHHCCCHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCH
EHLEYLETDPGKCIAKAYDMVLNGWEIGGGSVRIYREEVQSKVFRALKINDEEARAKFGF
HHHHHHCCCCCHHHHHHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHH
LLDALQYGAPPHGGIAFGLDRVVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDEKQL
HHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHH
RELHIRLRAAEAKVAAPTAATTA
HHHHHHHEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]