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The map label for this gene is ubiE

Identifier: 187927436

GI number: 187927436

Start: 353492

End: 354223

Strand: Direct

Name: ubiE

Synonym: Rpic_0333

Alternate gene names: 187927436

Gene position: 353492-354223 (Clockwise)

Preceding gene: 187927435

Following gene: 187927437

Centisome position: 8.97

GC content: 61.48

Gene sequence:

>732_bases
ATGAGCCAAACCCACTTCGGATTCCAGCAGGTCGATGAGCAGGAAAAGGCCGGCAAGGTTGCCGGCGTGTTTCATTCCGT
CGCGAGCAAGTACGACGTGATGAACGACCTGATGTCCGGCGGCCTGCACCGCGTGTGGAAAATGTTCACCATTGCCCAGG
CAGCAGTGCGGCCGGGCTACAAGGTGCTCGATATCGCTGGCGGCACGGGTGATCTGGCGAAGGCCTTCGCGCGCCAGGCA
GGTCCGACCGGCGAGGTCTGGCTGACCGACATCAACGAGTCGATGCTGCGCGTCGGCCGTGACCGGCTGCTCGATGCTGG
CGTGCTCACCCCCACCGCCCTGTGCGACGCCGAGCACATTCCCTTTCCGAATGCTTACTTCGATGTGGTGACAGTGGCAT
TCGGCTTGCGCAACATGACGCACAAGGATCGCGCGCTCGCGGAAATGCGTCGTGTGGTGAAGCCAGGGGGCAAGGTCATG
GTGCTGGAGTTCTCCAAGGTGTGGCAGCCACTGGAGAAGGCCTACGACGTGTACTCGTTCAAGGTGCTACCTTGGTTGGG
CAGCAAAGTGGCGGGCGACCCCGAGAGCTATCGTTACCTGGCCGAGTCCATCCGCATGCACCCGGATCAGGAAACGCTCA
AGCAGATGATGGAACAGGCCGGATTGGACTCGGTCGAATACTTCAATCTGACAGCAGGTGTCGTGGCTCTGCACGTCGGC
CGTCGCCTCTAG

Upstream 100 bases:

>100_bases
TCCGATGGCAGAGAACGCCGGCCACGCCTGCGGCCACAGTCACTGATTCCCGATTCATTTCGCGGCGCCCCTGTGCGCTG
CATCCGCATCCCTTTCTGTC

Downstream 100 bases:

>100_bases
GTGTTTTCGTGGTTCCACCAGATGGGCGATGCAGCGCGCTGCGGCGATATGCCACGATCCTTCACATTGAATTTCGGCGT
TGACGTCTTCATCTAATGGC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSQTHFGFQQVDEQEKAGKVAGVFHSVASKYDVMNDLMSGGLHRVWKMFTIAQAAVRPGYKVLDIAGGTGDLAKAFARQA
GPTGEVWLTDINESMLRVGRDRLLDAGVLTPTALCDAEHIPFPNAYFDVVTVAFGLRNMTHKDRALAEMRRVVKPGGKVM
VLEFSKVWQPLEKAYDVYSFKVLPWLGSKVAGDPESYRYLAESIRMHPDQETLKQMMEQAGLDSVEYFNLTAGVVALHVG
RRL

Sequences:

>Translated_243_residues
MSQTHFGFQQVDEQEKAGKVAGVFHSVASKYDVMNDLMSGGLHRVWKMFTIAQAAVRPGYKVLDIAGGTGDLAKAFARQA
GPTGEVWLTDINESMLRVGRDRLLDAGVLTPTALCDAEHIPFPNAYFDVVTVAFGLRNMTHKDRALAEMRRVVKPGGKVM
VLEFSKVWQPLEKAYDVYSFKVLPWLGSKVAGDPESYRYLAESIRMHPDQETLKQMMEQAGLDSVEYFNLTAGVVALHVG
RRL
>Mature_242_residues
SQTHFGFQQVDEQEKAGKVAGVFHSVASKYDVMNDLMSGGLHRVWKMFTIAQAAVRPGYKVLDIAGGTGDLAKAFARQAG
PTGEVWLTDINESMLRVGRDRLLDAGVLTPTALCDAEHIPFPNAYFDVVTVAFGLRNMTHKDRALAEMRRVVKPGGKVMV
LEFSKVWQPLEKAYDVYSFKVLPWLGSKVAGDPESYRYLAESIRMHPDQETLKQMMEQAGLDSVEYFNLTAGVVALHVGR
RL

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=278, Percent_Identity=45.6834532374101, Blast_Score=233, Evalue=1e-61,
Organism=Escherichia coli, GI48994983, Length=243, Percent_Identity=62.5514403292181, Blast_Score=311, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17557039, Length=242, Percent_Identity=45.0413223140496, Blast_Score=216, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6323526, Length=258, Percent_Identity=40.3100775193798, Blast_Score=164, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24641702, Length=256, Percent_Identity=47.265625, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_RALPJ (B2UFG8)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897923.1
- ProteinModelPortal:   B2UFG8
- SMR:   B2UFG8
- GeneID:   6289237
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0333
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 27009; Mature: 26878

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCC
>Mature Secondary Structure 
SQTHFGFQQVDEQEKAGKVAGVFHSVASKYDVMNDLMSGGLHRVWKMFTIAQAAVRPGY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KVLDIAGGTGDLAKAFARQAGPTGEVWLTDINESMLRVGRDRLLDAGVLTPTALCDAEHI
EEEEECCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PFPNAYFDVVTVAFGLRNMTHKDRALAEMRRVVKPGGKVMVLEFSKVWQPLEKAYDVYSF
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGSKVAGDPESYRYLAESIRMHPDQETLKQMMEQAGLDSVEYFNLTAGVVALHVG
EEHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RRL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA