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The map label for this gene is pfpI [H]

Identifier: 187927378

GI number: 187927378

Start: 285777

End: 286355

Strand: Reverse

Name: pfpI [H]

Synonym: Rpic_0271

Alternate gene names: 187927378

Gene position: 286355-285777 (Counterclockwise)

Preceding gene: 187927381

Following gene: 187927377

Centisome position: 7.26

GC content: 64.42

Gene sequence:

>579_bases
ATGGCAAAGAAAATTCTGATGCTGGTCGGCGACTACGTAGAGGACTACGAGGTCATGGTTCCCTTCCAGGCGCTGCAGAT
GGTCGGTCACACCGTGCACGCCGTCTGTCCGGACAAGCGCGCTGGCGATTCCGTGGCGACCGCCGTCCACGATTTCGAAG
GCGCACAGACGTATTCCGAAAAACCCGGTCATCGCTTCACCGTCAACGCCACCTTTGCAGATGTTGACCCGGCAGCGTAC
GACGCGCTGGTCGTGCCCGGCGGCCGCGCGCCCGAATACCTGCGCCTGAACGCCCGCGTGCTTGAGGTCGTACGTCACTT
CTTCCAGGCGGACAAACCCGTCGCAGCGATCTGCCACGGTGCCCAGTTGCTGTCGGCCGCGGGTGTGCTGGAGGGCAAGG
CGTGTTCGGCCTATCCGGCATGTGGCCCGGAAGTCACGGCAGCGGGCGGCACGTTCAAGGACATCCCCGTTGACCAGGCG
CACACCGACGGCAAGCTCGTCACGGCACCCGCCTGGCCGGCACATCCGGCCTGGTTGGCGCAGTTTCTTGAAGTCCTGGG
CACGCGGGTTCTACACTGA

Upstream 100 bases:

>100_bases
CAGGAAAGCAGGAAATGTGGTCTGCGGTAATGGGCTACCACCCGGGCCGAATGGCGCCGAACCACAATGGGCAAACCGCA
ACCATACAGGAGACACGGCG

Downstream 100 bases:

>100_bases
TCCGGCTGCCCGCACGCCGGGCACTCCAGGAGCCCCGGCCATGTGCGAAATCTTCATCCGCGCCAATCCCCATTCGTATG
ACAGCCTCGCGCGCTCGCTA

Product: PfpI family intracellular protease

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKRAGDSVATAVHDFEGAQTYSEKPGHRFTVNATFADVDPAAY
DALVVPGGRAPEYLRLNARVLEVVRHFFQADKPVAAICHGAQLLSAAGVLEGKACSAYPACGPEVTAAGGTFKDIPVDQA
HTDGKLVTAPAWPAHPAWLAQFLEVLGTRVLH

Sequences:

>Translated_192_residues
MAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKRAGDSVATAVHDFEGAQTYSEKPGHRFTVNATFADVDPAAY
DALVVPGGRAPEYLRLNARVLEVVRHFFQADKPVAAICHGAQLLSAAGVLEGKACSAYPACGPEVTAAGGTFKDIPVDQA
HTDGKLVTAPAWPAHPAWLAQFLEVLGTRVLH
>Mature_191_residues
AKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKRAGDSVATAVHDFEGAQTYSEKPGHRFTVNATFADVDPAAYD
ALVVPGGRAPEYLRLNARVLEVVRHFFQADKPVAAICHGAQLLSAAGVLEGKACSAYPACGPEVTAAGGTFKDIPVDQAH
TDGKLVTAPAWPAHPAWLAQFLEVLGTRVLH

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=189, Percent_Identity=34.3915343915344, Blast_Score=100, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 20504; Mature: 20372

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKRAGDSVATAVHDFEGAQTYSE
CCCHHEEHHHHHHHCCEEECCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHCCCCCCCCC
KPGHRFTVNATFADVDPAAYDALVVPGGRAPEYLRLNARVLEVVRHFFQADKPVAAICHG
CCCCEEEEEEEECCCCCCCCCEEEECCCCCCHHEEHHHHHHHHHHHHHHCCCCHHHHHHH
AQLLSAAGVLEGKACSAYPACGPEVTAAGGTFKDIPVDQAHTDGKLVTAPAWPAHPAWLA
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHH
QFLEVLGTRVLH
HHHHHHHHHHCC
>Mature Secondary Structure 
AKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKRAGDSVATAVHDFEGAQTYSE
CCHHEEHHHHHHHCCEEECCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHCCCCCCCCC
KPGHRFTVNATFADVDPAAYDALVVPGGRAPEYLRLNARVLEVVRHFFQADKPVAAICHG
CCCCEEEEEEEECCCCCCCCCEEEECCCCCCHHEEHHHHHHHHHHHHHHCCCCHHHHHHH
AQLLSAAGVLEGKACSAYPACGPEVTAAGGTFKDIPVDQAHTDGKLVTAPAWPAHPAWLA
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHH
QFLEVLGTRVLH
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]