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The map label for this gene is pth

Identifier: 187927355

GI number: 187927355

Start: 262553

End: 263155

Strand: Reverse

Name: pth

Synonym: Rpic_0248

Alternate gene names: 187927355

Gene position: 263155-262553 (Counterclockwise)

Preceding gene: 187927356

Following gene: 187927354

Centisome position: 6.67

GC content: 63.35

Gene sequence:

>603_bases
ATGATCAAACTCATCGTCGGCCTCGGCAATCCGGGCGCCGAATATGCGGCGACACGACACAACGCCGGCTTCTGGCTGGT
CGACCAACTCGCGCGCATGGGCAACGTGACGCTGCGCAACGAGACGCGCTTCCACGGCTATGCAGCCCGCGCCAACCTTT
GGGGCAACGAGGTGTGGCTGCTCCAGCCGCAAACCTTCATGAACCGCTCCGGCTTGGCCACCGTGGCGCTGGCACGCTTC
TACAAGGTGATGCCCGATGAAATCCTGGTCGTGCATGACGAATTGGATTTGCCGCCCGGCACCGTCAAGCTCAAGCTCGG
TGGCGGATCCGGTGGCCACAACGGCCTGAAGGACATTGCCGCACATCTGACCACGCAACAGTTCTGGCGGCTGCGCCTTG
GCATCGGCCATCCGCGCAACCTGTTGCCGCCGGGCACGGCAGCGTCCGGTCAGCATGACGTCGCCAACTTCGTGCTCAAA
GCGCCTCGCAAGGAAGAACAGGACCTCATCGACCGCGCCATCGACCAGAGCCTCGATGCGCTGCCTGAGCTGATTGCCGG
CAACGCCGAGAAAGCGATGATGCGCCTGCATACCGCACACTGA

Upstream 100 bases:

>100_bases
TGCCTGAGCGGCCCGAGCCGCCCCGTCTCCAGGCTGTAGAAAGCAGCTATGCTTGTACGATCAAGGGGCGCTTCCTGCAC
CTGACACTGATACGAATCGC

Downstream 100 bases:

>100_bases
TCCGCCAGCACTGAGGACACCGCCATGACACCGTTCGCCATACTGACCTCACGCCCCGCCGTGCTGGCGGGTCTGGCGCT
CGGACTTGCAGCCAGCCTGC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MIKLIVGLGNPGAEYAATRHNAGFWLVDQLARMGNVTLRNETRFHGYAARANLWGNEVWLLQPQTFMNRSGLATVALARF
YKVMPDEILVVHDELDLPPGTVKLKLGGGSGGHNGLKDIAAHLTTQQFWRLRLGIGHPRNLLPPGTAASGQHDVANFVLK
APRKEEQDLIDRAIDQSLDALPELIAGNAEKAMMRLHTAH

Sequences:

>Translated_200_residues
MIKLIVGLGNPGAEYAATRHNAGFWLVDQLARMGNVTLRNETRFHGYAARANLWGNEVWLLQPQTFMNRSGLATVALARF
YKVMPDEILVVHDELDLPPGTVKLKLGGGSGGHNGLKDIAAHLTTQQFWRLRLGIGHPRNLLPPGTAASGQHDVANFVLK
APRKEEQDLIDRAIDQSLDALPELIAGNAEKAMMRLHTAH
>Mature_200_residues
MIKLIVGLGNPGAEYAATRHNAGFWLVDQLARMGNVTLRNETRFHGYAARANLWGNEVWLLQPQTFMNRSGLATVALARF
YKVMPDEILVVHDELDLPPGTVKLKLGGGSGGHNGLKDIAAHLTTQQFWRLRLGIGHPRNLLPPGTAASGQHDVANFVLK
APRKEEQDLIDRAIDQSLDALPELIAGNAEKAMMRLHTAH

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=184, Percent_Identity=35.8695652173913, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI1787455, Length=197, Percent_Identity=54.3147208121827, Blast_Score=176, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_RALPJ (B2UER4)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897842.1
- ProteinModelPortal:   B2UER4
- SMR:   B2UER4
- GeneID:   6288148
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0248
- HOGENOM:   HBG610927
- OMA:   SHAGHNG
- ProtClustDB:   PRK05426
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21964; Mature: 21964

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLIVGLGNPGAEYAATRHNAGFWLVDQLARMGNVTLRNETRFHGYAARANLWGNEVWL
CEEEEEECCCCCHHHHECCCCCCCHHHHHHHHHCCEEECCCCCCCCEEEECCCCCCEEEE
LQPQTFMNRSGLATVALARFYKVMPDEILVVHDELDLPPGTVKLKLGGGSGGHNGLKDIA
ECCHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHH
AHLTTQQFWRLRLGIGHPRNLLPPGTAASGQHDVANFVLKAPRKEEQDLIDRAIDQSLDA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LPELIAGNAEKAMMRLHTAH
HHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIKLIVGLGNPGAEYAATRHNAGFWLVDQLARMGNVTLRNETRFHGYAARANLWGNEVWL
CEEEEEECCCCCHHHHECCCCCCCHHHHHHHHHCCEEECCCCCCCCEEEECCCCCCEEEE
LQPQTFMNRSGLATVALARFYKVMPDEILVVHDELDLPPGTVKLKLGGGSGGHNGLKDIA
ECCHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHH
AHLTTQQFWRLRLGIGHPRNLLPPGTAASGQHDVANFVLKAPRKEEQDLIDRAIDQSLDA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LPELIAGNAEKAMMRLHTAH
HHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA