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The map label for this gene is nrtB [H]

Identifier: 187927343

GI number: 187927343

Start: 250963

End: 251814

Strand: Reverse

Name: nrtB [H]

Synonym: Rpic_0236

Alternate gene names: 187927343

Gene position: 251814-250963 (Counterclockwise)

Preceding gene: 187927344

Following gene: 187927342

Centisome position: 6.39

GC content: 64.32

Gene sequence:

>852_bases
ATGAACACGCTCGTCAAAACGTTGTCCGGCGTGCAGTCCGACGCCGCCCCTGAAAGCCGCCGCTTTGCCGTGCGCGACTG
GTTTGTCGCCGCCGTGGTGCGCGGCTTTCCGCCCGTGCTGGGCTTGGTTCTGTTTGTACTGGCGTGGCAGGGCATTGCCA
CGGCCATCCCCGCATTGCCGACACCAGCGGTGACGTGGAAAGCAGCGGTCGCGTTGTTTGCCGATCCGTTCTACCGCAAT
GGCCCGAATGACCAGGGCGTGGGCTGGAACGTGCTCGCTTCGCTGGCGCGCGTGGGTGCGGGCTTCGGCATGGCTGCAGT
CATCGGCATTCCGGCGGGCTTCCTCATCGGGCGTTTTGCGTTTCTCAACGCGATGGCCTCGCCCATCATCAGCCTGCTGC
GGCCCGTGTCGCCGCTGGCGTGGCTGCCGATTGGCCTCTTGCTATTCAAGTCGGCCAATCCGGCGGCCATCTGGGCGATC
TTCATCTGCTCGATCTGGCCGATGGTGATCAACACCGCCGTGGGCGTGACGCGCGTGCCGCAGGACTACCTGAACGTGGC
GCGCGTGCTGAACCTGTCTGAGTGGAAGGTGTTCACGCGCGTGCTATTTCCCGCCGTGCTGCCCTATATGCTGACGGGTG
TGCGATTGTCCATCGGCACGGCGTGGCTGGTCATCGTGGCGGCGGAGATGCTCACCGGCGGTGTCGGCATCGGCTTCTGG
CTGTGGGATGAGTGGAACAACCTGAAGGTCGAACACATCGTGATCGCGATCTTCGTGATTGGCGTGGTGGGCTTGCTGCT
CGAGCACGCGCTGCTGGCGATTGCGCGGCGCTTCTCGTACGACGCGGTTTGA

Upstream 100 bases:

>100_bases
AAGAACCCTGCCGCGTATGCGGACGGCTTCAAGCTGAAAGCCTGACGCCTCCGGCCTTCCGAATTTCTGATTGCGGTCCC
TCACATCGATGGAGCCCGAC

Downstream 100 bases:

>100_bases
GCGAAATGGGGATCGCCATGACAGACAAATTCCTGCGCATTGAAAACGTCGGGCAGACGTTCAAGACCAAGCGCGGCCCG
TTCGTCGCCCTGCGCGACAT

Product: nitrate ABC transporter inner membrane subunit

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNTLVKTLSGVQSDAAPESRRFAVRDWFVAAVVRGFPPVLGLVLFVLAWQGIATAIPALPTPAVTWKAAVALFADPFYRN
GPNDQGVGWNVLASLARVGAGFGMAAVIGIPAGFLIGRFAFLNAMASPIISLLRPVSPLAWLPIGLLLFKSANPAAIWAI
FICSIWPMVINTAVGVTRVPQDYLNVARVLNLSEWKVFTRVLFPAVLPYMLTGVRLSIGTAWLVIVAAEMLTGGVGIGFW
LWDEWNNLKVEHIVIAIFVIGVVGLLLEHALLAIARRFSYDAV

Sequences:

>Translated_283_residues
MNTLVKTLSGVQSDAAPESRRFAVRDWFVAAVVRGFPPVLGLVLFVLAWQGIATAIPALPTPAVTWKAAVALFADPFYRN
GPNDQGVGWNVLASLARVGAGFGMAAVIGIPAGFLIGRFAFLNAMASPIISLLRPVSPLAWLPIGLLLFKSANPAAIWAI
FICSIWPMVINTAVGVTRVPQDYLNVARVLNLSEWKVFTRVLFPAVLPYMLTGVRLSIGTAWLVIVAAEMLTGGVGIGFW
LWDEWNNLKVEHIVIAIFVIGVVGLLLEHALLAIARRFSYDAV
>Mature_283_residues
MNTLVKTLSGVQSDAAPESRRFAVRDWFVAAVVRGFPPVLGLVLFVLAWQGIATAIPALPTPAVTWKAAVALFADPFYRN
GPNDQGVGWNVLASLARVGAGFGMAAVIGIPAGFLIGRFAFLNAMASPIISLLRPVSPLAWLPIGLLLFKSANPAAIWAI
FICSIWPMVINTAVGVTRVPQDYLNVARVLNLSEWKVFTRVLFPAVLPYMLTGVRLSIGTAWLVIVAAEMLTGGVGIGFW
LWDEWNNLKVEHIVIAIFVIGVVGLLLEHALLAIARRFSYDAV

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=200, Percent_Identity=32, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081802, Length=139, Percent_Identity=30.2158273381295, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30544; Mature: 30544

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLVKTLSGVQSDAAPESRRFAVRDWFVAAVVRGFPPVLGLVLFVLAWQGIATAIPALP
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
TPAVTWKAAVALFADPFYRNGPNDQGVGWNVLASLARVGAGFGMAAVIGIPAGFLIGRFA
CCCHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHH
FLNAMASPIISLLRPVSPLAWLPIGLLLFKSANPAAIWAIFICSIWPMVINTAVGVTRVP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCC
QDYLNVARVLNLSEWKVFTRVLFPAVLPYMLTGVRLSIGTAWLVIVAAEMLTGGVGIGFW
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LWDEWNNLKVEHIVIAIFVIGVVGLLLEHALLAIARRFSYDAV
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNTLVKTLSGVQSDAAPESRRFAVRDWFVAAVVRGFPPVLGLVLFVLAWQGIATAIPALP
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
TPAVTWKAAVALFADPFYRNGPNDQGVGWNVLASLARVGAGFGMAAVIGIPAGFLIGRFA
CCCHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHH
FLNAMASPIISLLRPVSPLAWLPIGLLLFKSANPAAIWAIFICSIWPMVINTAVGVTRVP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCC
QDYLNVARVLNLSEWKVFTRVLFPAVLPYMLTGVRLSIGTAWLVIVAAEMLTGGVGIGFW
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
LWDEWNNLKVEHIVIAIFVIGVVGLLLEHALLAIARRFSYDAV
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]