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The map label for this gene is rpoH [H]

Identifier: 187927337

GI number: 187927337

Start: 243366

End: 244295

Strand: Reverse

Name: rpoH [H]

Synonym: Rpic_0230

Alternate gene names: 187927337

Gene position: 244295-243366 (Counterclockwise)

Preceding gene: 187927338

Following gene: 187927331

Centisome position: 6.2

GC content: 63.12

Gene sequence:

>930_bases
GTGAACGCAGTCATCCCCGTGAATACCGTTTCCGCAATCGCCACCCAGCCGTCCGGCAACGCCGGTAATGGATGGGCACT
GGCGTTCCCCGGCAACCTGGGCAACATCGACGCCTATATCCAGAGCGTCAACCGTGTGCCGATGTTGTCGGCCGAAGAAG
AACAGCGTCTGGCCCGCGAATACCGCGACAACGACAACCTCGATGCCGCTCGCCGACTGGTGCTCTCGCACCTGCGTCTG
GTGGTGTCGATCGCCCGCGGCTACCTCGGCTATGGCCTGCCGCATGCCGACCTCATCCAGGAAGGCAACATCGGCCTGAT
GAAGGCCGTCAAGCGCTTCGACCCGGATCAGGGCGTGCGTCTCGTCTCATACGCGATGCACTGGATCAAGGCCGAGATGC
ACGAGTACATCCTGAAGAACTGGCGCATGGTCAAGGTGGCCACCACCAAGGCCCAGCGCAAGCTGTTCTTCAACCTGCGC
AGTCACAAGCAGGATGCGCAGGCGACCTTCACGTCGGACGAGATCGACGCCGTGGCGGCCGAACTCAACGTCAAGGGCAC
GGAAGTGCGCGAGATGGAAACGCGCCTGTCCGGCGGCGACATTGCGCTGGAAGGCCAGATGGACGACGGTGAGGAGTCGT
TCGCTCCCATCGCCTACCTGGCCGATACGCACAACGAGCCGACCCGCGTGATCGAAGCGCGTAGCAACGACCGCATGCAA
AGCGAGGGCATCGAAGCCGCCCTCGCCAAGCTGGACGATCGCAGCCGCCGCATCATCGAAGCACGCTGGCTGAACGTGAA
CGACGACGGATCGGGCGGCTCGACGCTGCACGAGTTGGCCGACGAGTTTGGCGTGTCGGCTGAGCGCATTCGCCAGATCG
AATCGGCCGCGATGAAAAAGATGAAGGGTGCGCTGCAGGCCTTCGCCTAA

Upstream 100 bases:

>100_bases
AGGAACTATCAACCCGACAAAAATGTCCTATTAGCACTCATCCGGTTGGAGTGCTAAGATGCCTTTCCAAATCTCCACTG
GATTTCGACAGGAGTCCTGA

Downstream 100 bases:

>100_bases
GCTGCCCGCCCTGCAATGAAAAGCCCGCCAAATTGGCGGGCTTTTTCTTTGGGCGGTGGCGGAAGATCAGTCGGCCAGCA
GTTGCTTGATGTCGTGCGCG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNAVIPVNTVSAIATQPSGNAGNGWALAFPGNLGNIDAYIQSVNRVPMLSAEEEQRLAREYRDNDNLDAARRLVLSHLRL
VVSIARGYLGYGLPHADLIQEGNIGLMKAVKRFDPDQGVRLVSYAMHWIKAEMHEYILKNWRMVKVATTKAQRKLFFNLR
SHKQDAQATFTSDEIDAVAAELNVKGTEVREMETRLSGGDIALEGQMDDGEESFAPIAYLADTHNEPTRVIEARSNDRMQ
SEGIEAALAKLDDRSRRIIEARWLNVNDDGSGGSTLHELADEFGVSAERIRQIESAAMKKMKGALQAFA

Sequences:

>Translated_309_residues
MNAVIPVNTVSAIATQPSGNAGNGWALAFPGNLGNIDAYIQSVNRVPMLSAEEEQRLAREYRDNDNLDAARRLVLSHLRL
VVSIARGYLGYGLPHADLIQEGNIGLMKAVKRFDPDQGVRLVSYAMHWIKAEMHEYILKNWRMVKVATTKAQRKLFFNLR
SHKQDAQATFTSDEIDAVAAELNVKGTEVREMETRLSGGDIALEGQMDDGEESFAPIAYLADTHNEPTRVIEARSNDRMQ
SEGIEAALAKLDDRSRRIIEARWLNVNDDGSGGSTLHELADEFGVSAERIRQIESAAMKKMKGALQAFA
>Mature_309_residues
MNAVIPVNTVSAIATQPSGNAGNGWALAFPGNLGNIDAYIQSVNRVPMLSAEEEQRLAREYRDNDNLDAARRLVLSHLRL
VVSIARGYLGYGLPHADLIQEGNIGLMKAVKRFDPDQGVRLVSYAMHWIKAEMHEYILKNWRMVKVATTKAQRKLFFNLR
SHKQDAQATFTSDEIDAVAAELNVKGTEVREMETRLSGGDIALEGQMDDGEESFAPIAYLADTHNEPTRVIEARSNDRMQ
SEGIEAALAKLDDRSRRIIEARWLNVNDDGSGGSTLHELADEFGVSAERIRQIESAAMKKMKGALQAFA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=277, Percent_Identity=54.1516245487365, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1789098, Length=273, Percent_Identity=30.4029304029304, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789448, Length=239, Percent_Identity=30.5439330543933, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34238; Mature: 34238

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVIPVNTVSAIATQPSGNAGNGWALAFPGNLGNIDAYIQSVNRVPMLSAEEEQRLARE
CCCEECCCHHHHHEECCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
YRDNDNLDAARRLVLSHLRLVVSIARGYLGYGLPHADLIQEGNIGLMKAVKRFDPDQGVR
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHCCCCCCHH
LVSYAMHWIKAEMHEYILKNWRMVKVATTKAQRKLFFNLRSHKQDAQATFTSDEIDAVAA
HHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHH
ELNVKGTEVREMETRLSGGDIALEGQMDDGEESFAPIAYLADTHNEPTRVIEARSNDRMQ
HHCCCCCHHHHHHHHCCCCCEEEECCCCCCCHHCCCCCEEECCCCCCHHEEECCCCCCHH
SEGIEAALAKLDDRSRRIIEARWLNVNDDGSGGSTLHELADEFGVSAERIRQIESAAMKK
HHHHHHHHHHHHHHHHHEEHHEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MKGALQAFA
HHHHHHHCC
>Mature Secondary Structure
MNAVIPVNTVSAIATQPSGNAGNGWALAFPGNLGNIDAYIQSVNRVPMLSAEEEQRLARE
CCCEECCCHHHHHEECCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
YRDNDNLDAARRLVLSHLRLVVSIARGYLGYGLPHADLIQEGNIGLMKAVKRFDPDQGVR
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHCCCCCCHH
LVSYAMHWIKAEMHEYILKNWRMVKVATTKAQRKLFFNLRSHKQDAQATFTSDEIDAVAA
HHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHH
ELNVKGTEVREMETRLSGGDIALEGQMDDGEESFAPIAYLADTHNEPTRVIEARSNDRMQ
HHCCCCCHHHHHHHHCCCCCEEEECCCCCCCHHCCCCCEEECCCCCCHHEEECCCCCCHH
SEGIEAALAKLDDRSRRIIEARWLNVNDDGSGGSTLHELADEFGVSAERIRQIESAAMKK
HHHHHHHHHHHHHHHHHEEHHEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MKGALQAFA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]