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The map label for this gene is phbI [H]

Identifier: 187927312

GI number: 187927312

Start: 221835

End: 223586

Strand: Direct

Name: phbI [H]

Synonym: Rpic_0204

Alternate gene names: 187927312

Gene position: 221835-223586 (Clockwise)

Preceding gene: 187927311

Following gene: 187927313

Centisome position: 5.63

GC content: 64.5

Gene sequence:

>1752_bases
ATGCCTTTTACCCTTCACGGTATTCCGGTCTCGCGCGGCATCTCCATCGGCCGCGCCCACATGCTGGCGCGCGCGGCGCT
CGATGTGTCGCACTACCTGGTCGACGAAGACAAGCTTGACGCTGAGGTCGAGCGCTTGCGCAGTGCCCGCGCCACCGTAC
GCTCCGAACTGGCCGCACTCAAGCGCGACCTCCCGAGCGATGTGCCCGAAGAGATGGGTGCCTTCCTCGATGTGCACGCG
ATGATTTTGGATGACGCGTTGCTCTCGCAGGTACCCGAGACGCTGATTCGCCAGCGGCGCTACAACGCAGAGTGGGCGCT
CACAACGCAGCTTGAAGAGCTTATCCGCCAGTTCGGTGAAATCGAAGACGAATACCTGCGCGAGCGCAAGGCCGACATCG
AGCAGGTGGTTGAACGCATTCTGAAGGTACTGGCCGGCGCGCCGGCCTTGGCGCCGGCACCGGTGGCCGTTGGCTGCGAT
CCGGATGCCGGCATGATCGTCGTCGCACACGATATTGCGCCGGCCGACATGCTGCAGTTCCGCGGCCAGACCTTCGCCGG
CTTCGTGACCGACCTGGGCGGCCGCACCTCGCACACCGCCATCGTGGCGCGTAGCCTCGACATTCCGGCGGCGGTAGGTG
TGCATAACGCGAGTACGCTGATTCGGCAGGATGACATCGTCGTCATTGACGGCGACAACGGCATCGTCATTGTCGATCCG
AGCACGTCCATCCTTGAGGAATACCGCCACCGCCGCAGCGAAGGCGAGCTGCAGAAGAAGCGTCTCGAACGTCTGCGTCA
TACGCCTACCGTTACACTCGACGGTACGCAAATCGAACTGCAGGCGAACATCGAAATGCCCGAAGATGCGGCTGCCGCTG
TCAAAGCCGGCGCCGTGGGCGTGGGCCTGTTCCGCTCCGAATTCCTCTTCATGAACCGTCGTGGCGCGCTACCTGACGAG
GAAGAACAGTTCCGCGCGTACCGTACCGCCGTTGAATCGATGATGGGCCTGCCCGTCACCATCCGCACCATCGACATCGG
TGCCGACAAGCCGCTGGACATCCGCGACGACGTGTTCGAGACCGCGCCCAATCCGGCGCTGGGTTTGCGGGCGATCCGCT
GGTCGCTGTCAGAGCCGGCGATGTTCCTGACGCAGTTGCGCGCGCTGCTGCGGGCGTCGGCATTTGGGCCGATCAAGATT
CTCGTGCCGATGTTGGCGCACGCCCGCGAAATCGACCAGACCCTGGAATTGCTGGCGCGCGCCAAGGCTTCGCTCGACGC
CGACGGCCTGGCCTATGATCCGGGCATCAAGGTCGGTGCCATGATCGAGATACCGGCCGCCGTGCTGATCCTGCCAGTGT
TCCTGCGCCGCATGGATTTTCTCTCGATCGGCACGAACGACCTGATCCAGTACACGCTCGCCATCGATCGCGCCGACAAC
GCCGTCGCGCATCTGTACGACCCGCTGCATCCAGCGGTGCTGCAATTGATCGCGCGCACCATCCGCGAAGCGCATCAGGC
GGGCAAGCCCGTTTCCGTGTGCGGCGAGATGGCGGGCGACGCTTCCATGACGCGCCTGCTGCTCGGCATGGGCCTCACCG
AATTCTCGATGCATCCGTCGCAGTTGCTGAGTGTCAAGCAGCAGATCCTGCGCTCGGACCGCCCGCGCTTGATGCGCGAG
GCCGACCGCCTGCTGCACGCCTGCGAGCCGGCCGAAATCGAAGAGGCGCTCGGCGCGCTGGCGCGGGCTTGA

Upstream 100 bases:

>100_bases
CGCCATGGATGGTCTCGTCGCGCTCATCAACGATCGCTTTGGCGAAGGCGAATAAAGCGCCCGCCGCACTCCACTTCAAC
GCCCGCTCCGGCCAGCGTCT

Downstream 100 bases:

>100_bases
TGCGCGCATCTCAAAAGCGCCGGCTGCACCGAGTGTCGCACCGGCACATCGCCTTCCACGGGCATGACCTCGCTCTAAGT
GACTGAAATTGCACGTCTTT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MPFTLHGIPVSRGISIGRAHMLARAALDVSHYLVDEDKLDAEVERLRSARATVRSELAALKRDLPSDVPEEMGAFLDVHA
MILDDALLSQVPETLIRQRRYNAEWALTTQLEELIRQFGEIEDEYLRERKADIEQVVERILKVLAGAPALAPAPVAVGCD
PDAGMIVVAHDIAPADMLQFRGQTFAGFVTDLGGRTSHTAIVARSLDIPAAVGVHNASTLIRQDDIVVIDGDNGIVIVDP
STSILEEYRHRRSEGELQKKRLERLRHTPTVTLDGTQIELQANIEMPEDAAAAVKAGAVGVGLFRSEFLFMNRRGALPDE
EEQFRAYRTAVESMMGLPVTIRTIDIGADKPLDIRDDVFETAPNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPIKI
LVPMLAHAREIDQTLELLARAKASLDADGLAYDPGIKVGAMIEIPAAVLILPVFLRRMDFLSIGTNDLIQYTLAIDRADN
AVAHLYDPLHPAVLQLIARTIREAHQAGKPVSVCGEMAGDASMTRLLLGMGLTEFSMHPSQLLSVKQQILRSDRPRLMRE
ADRLLHACEPAEIEEALGALARA

Sequences:

>Translated_583_residues
MPFTLHGIPVSRGISIGRAHMLARAALDVSHYLVDEDKLDAEVERLRSARATVRSELAALKRDLPSDVPEEMGAFLDVHA
MILDDALLSQVPETLIRQRRYNAEWALTTQLEELIRQFGEIEDEYLRERKADIEQVVERILKVLAGAPALAPAPVAVGCD
PDAGMIVVAHDIAPADMLQFRGQTFAGFVTDLGGRTSHTAIVARSLDIPAAVGVHNASTLIRQDDIVVIDGDNGIVIVDP
STSILEEYRHRRSEGELQKKRLERLRHTPTVTLDGTQIELQANIEMPEDAAAAVKAGAVGVGLFRSEFLFMNRRGALPDE
EEQFRAYRTAVESMMGLPVTIRTIDIGADKPLDIRDDVFETAPNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPIKI
LVPMLAHAREIDQTLELLARAKASLDADGLAYDPGIKVGAMIEIPAAVLILPVFLRRMDFLSIGTNDLIQYTLAIDRADN
AVAHLYDPLHPAVLQLIARTIREAHQAGKPVSVCGEMAGDASMTRLLLGMGLTEFSMHPSQLLSVKQQILRSDRPRLMRE
ADRLLHACEPAEIEEALGALARA
>Mature_582_residues
PFTLHGIPVSRGISIGRAHMLARAALDVSHYLVDEDKLDAEVERLRSARATVRSELAALKRDLPSDVPEEMGAFLDVHAM
ILDDALLSQVPETLIRQRRYNAEWALTTQLEELIRQFGEIEDEYLRERKADIEQVVERILKVLAGAPALAPAPVAVGCDP
DAGMIVVAHDIAPADMLQFRGQTFAGFVTDLGGRTSHTAIVARSLDIPAAVGVHNASTLIRQDDIVVIDGDNGIVIVDPS
TSILEEYRHRRSEGELQKKRLERLRHTPTVTLDGTQIELQANIEMPEDAAAAVKAGAVGVGLFRSEFLFMNRRGALPDEE
EQFRAYRTAVESMMGLPVTIRTIDIGADKPLDIRDDVFETAPNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPIKIL
VPMLAHAREIDQTLELLARAKASLDADGLAYDPGIKVGAMIEIPAAVLILPVFLRRMDFLSIGTNDLIQYTLAIDRADNA
VAHLYDPLHPAVLQLIARTIREAHQAGKPVSVCGEMAGDASMTRLLLGMGLTEFSMHPSQLLSVKQQILRSDRPRLMREA
DRLLHACEPAEIEEALGALARA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=549, Percent_Identity=38.7978142076503, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1789193, Length=589, Percent_Identity=32.088285229202, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI48994992, Length=479, Percent_Identity=35.6993736951983, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1788726, Length=579, Percent_Identity=31.9516407599309, Blast_Score=255, Evalue=7e-69,
Organism=Escherichia coli, GI1787994, Length=444, Percent_Identity=25.9009009009009, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI226510935, Length=170, Percent_Identity=27.0588235294118, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63961; Mature: 63829

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFTLHGIPVSRGISIGRAHMLARAALDVSHYLVDEDKLDAEVERLRSARATVRSELAAL
CCEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRDLPSDVPEEMGAFLDVHAMILDDALLSQVPETLIRQRRYNAEWALTTQLEELIRQFGE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCC
IEDEYLRERKADIEQVVERILKVLAGAPALAPAPVAVGCDPDAGMIVVAHDIAPADMLQF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHH
RGQTFAGFVTDLGGRTSHTAIVARSLDIPAAVGVHNASTLIRQDDIVVIDGDNGIVIVDP
CCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCHHHHEECCCEEEEECCCCEEEECC
STSILEEYRHRRSEGELQKKRLERLRHTPTVTLDGTQIELQANIEMPEDAAAAVKAGAVG
CHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCHHHHHHHHCHHH
VGLFRSEFLFMNRRGALPDEEEQFRAYRTAVESMMGLPVTIRTIDIGADKPLDIRDDVFE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHH
TAPNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPIKILVPMLAHAREIDQTLELLAR
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKASLDADGLAYDPGIKVGAMIEIPAAVLILPVFLRRMDFLSIGTNDLIQYTLAIDRADN
HHHCCCCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHEEECCCC
AVAHLYDPLHPAVLQLIARTIREAHQAGKPVSVCGEMAGDASMTRLLLGMGLTEFSMHPS
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCCHHHCCCHH
QLLSVKQQILRSDRPRLMREADRLLHACEPAEIEEALGALARA
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PFTLHGIPVSRGISIGRAHMLARAALDVSHYLVDEDKLDAEVERLRSARATVRSELAAL
CEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KRDLPSDVPEEMGAFLDVHAMILDDALLSQVPETLIRQRRYNAEWALTTQLEELIRQFGE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCC
IEDEYLRERKADIEQVVERILKVLAGAPALAPAPVAVGCDPDAGMIVVAHDIAPADMLQF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHH
RGQTFAGFVTDLGGRTSHTAIVARSLDIPAAVGVHNASTLIRQDDIVVIDGDNGIVIVDP
CCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCHHHHEECCCEEEEECCCCEEEECC
STSILEEYRHRRSEGELQKKRLERLRHTPTVTLDGTQIELQANIEMPEDAAAAVKAGAVG
CHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCHHHHHHHHCHHH
VGLFRSEFLFMNRRGALPDEEEQFRAYRTAVESMMGLPVTIRTIDIGADKPLDIRDDVFE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHH
TAPNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPIKILVPMLAHAREIDQTLELLAR
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AKASLDADGLAYDPGIKVGAMIEIPAAVLILPVFLRRMDFLSIGTNDLIQYTLAIDRADN
HHHCCCCCCCEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHEEECCCC
AVAHLYDPLHPAVLQLIARTIREAHQAGKPVSVCGEMAGDASMTRLLLGMGLTEFSMHPS
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCCHHHCCCHH
QLLSVKQQILRSDRPRLMREADRLLHACEPAEIEEALGALARA
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]