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The map label for this gene is metH [H]

Identifier: 187927255

GI number: 187927255

Start: 164645

End: 167362

Strand: Reverse

Name: metH [H]

Synonym: Rpic_0147

Alternate gene names: 187927255

Gene position: 167362-164645 (Counterclockwise)

Preceding gene: 187927256

Following gene: 187927254

Centisome position: 4.25

GC content: 63.5

Gene sequence:

>2718_bases
ATGACCGACCACCCCATGCGCCTCTCTGGCCTCGAGCCGTTCAACATCGGCGAGAGCACGCTGTTCGTCAACGTCGGCGA
GCGCACCAACGTCACCGGGTCCAAGGCGTTCGCGCGCATGATCCTGAACAGCCAGTTCGACGAGGCGCTGGCCGTGGCGC
GCCAGCAGGTCGAAAACGGCGCGCAGGTCATCGACATCAACATGGACGAGGCGATGCTCGATTCCAAGGCGGCGATGGTG
CGCTTCCTGAATCTGATCGCCTCGGAGCCCGACATCGCGCGCGTGCCGATCATGATCGACTCGTCCAAATGGGACGTGAT
CGAGGCGGGTCTGAAGTGCGTGCAGGGCAAGGCGATCGTCAACTCGATCTCGCTCAAGGAAGGCGAAGAACAGTTCGCGC
ACCACGCCAAGCTGATCAAGCGCTACGGCGCCGCTGCCGTGGTGATGGCGTTTGACGAGCAAGGCCAGGCTGACACCTAT
GCGCGCAAGACCGAGATCTGCAAGCGCAGCTACGACTTCCTCGTCAACAAAGTCGGCTTTGCGCCGGAAGACATCATCTT
CGACCCGAACATCTTTGCGGTGGCGACCGGGATCGAGGAACACAACAACTATGCGGTGGACTTCATCGAAGCCACGCGCT
GGATCAAACAAAACCTGCCGTACGCGAAGGTGAGCGGCGGCGTGTCGAACGTGTCGTTTTCGTTCCGCGGCAACGACGTG
GTGCGCGAGGCGATCCACACCGTGTTCCTGTACTACGCAATTCAGGCGGGCATGGACATGGGCATCGTCAACGCCGGGCA
GTTGGGCGTGTACGAGAACCTCGACCCCGAACTGCGCGACCGCGTGGAAGACGTGGTGCTCAACCGCCGCCCCGATGCCA
CCGACCGCTTGCTGGAAATTGCGGACCGCTACAAGGGCGGCGGCACCAAGCGTGAAGAAAACCTCGCCTGGCGTCAGGAG
CCGGTGGAAAAGCGCCTGGCACATGCGCTCGTGCACGGCATTACCGACTACGTGGTCGAAGACACCGAAGAAGTTCGGCA
GAAGATCTTTGCCGCCGGCGGCCGCCCGATCCAGGTCATCGAAGGCCCGCTGATGGACGGCATGAACATCGTCGGTGACC
TCTTCGGCGCCGGGAAGATGTTCCTGCCGCAGGTGGTGAAATCCGCCCGCGTGATGAAGCAGGCGGTGGCCCACCTGATT
CCGTTCATCGAAGAAGAGAAGCGCAAGATTGCCGCGGCCGGCGGCGACGTGCGTTCGCGTGGCAAGATCGTCATCGCCAC
GGTCAAGGGCGACGTGCACGACATCGGCAAGAACATCGTCACCGTCGTGCTCCAGTGCAACAACTTCGAAGTCGTGAACA
TGGGCGTGATGGTCCCGTGCAACGACATCCTGGCCAAGGCGAAGGTGGAAGGCGCGGACATCATCGGCCTGTCGGGCCTG
ATTACGCCGTCGCTCGAAGAAATGGCCTACGTGGCGTCGGAAATGCAGCGCGACGAGTACTTCCGCGTGAAGAAAATCCC
GCTGCTGATCGGTGGCGCGACGACGAGCCGCGTGCATACCGCCGTGAAGATCGCCCCGAATTACGAAGGGCCGGTGGTTT
ACGTGCCGGACGCCTCGCGCTCAGTGAGCGTGGCGTCGAGCCTGCTGTCCGACGAAGGCGCCGCGCGCTACGTCGAAGAA
CTGCACGCCGACTACGACCGCATCCGCACGCAGCACGCCAATAAGAAAGCCACGCCGATGGTGTCGCTGGCCGCAGCGCG
CGCCAACAAGACGAAGATCGACTGGAGCAACTACACGCCGCCCAAGCCGAAGTTCATCGGCCGCCGCGTGTTCCGCAACT
ACGATCTGAACGAACTCGCCCAGTACATCGATTGGGGCCCGTTCTTCCAGACGTGGGACCTGGCCGGCAAATTCCCGGAC
ATTCTCAACGATGAGATCGTGGGTGAGTCGGCCCGTCGGGTGTTCTCCGACGGCAAGAGCATGCTCGCGCGCCTGATCGC
CGGGCGCTGGCTCACGGCCAACGGCGTGATCGCGCTGCTGCCCGCCAACACCGTCAACGACGACGACATCGAGATCTACA
CCGACGAATCGCGCTCGGAAGTCGCGCTCACCTGGCGCAACATCCGCCAGCAGAGCGAGCGCCCGATCATCGACGGCGTG
ATGCGCCCGAACCGCTGCCTGGCCGATTTCATCGCGCCCAAGGACACCGGCATTGCCGACTACATCGGCCTGTTTGCCGT
GACGGGCGGCATCGGGGTGGAAAAGCGCGAGAAGCAGTTCGAAGCCGACAACGACGACTACAGCGCGATCATGCTCAAGG
CGCTGGCCGACCGCTTTGCCGAAGCCTTTGCCGAATGCCTGCACGCCCGCGTGCGTCGCGACTTGTGGGGCTACGCACCG
GATGAGACGCTCGACAACGATGCGCTCATCCGCGAGGAATACAAGGGCATCCGCCCCGCGCCCGGCTACCCGGCCTGCCC
GGAGCACACCGTCAAGCGCGACATGTTCCGCGTGCTCGACGCGCAGGAGATCGGCATGGACCTGACCGAGGCGCTGGCCA
TGACGCCGGCCGCTTCCGTCTCGGGCTTCTACCTTTCGCATCCGGACAGCACGTACTTCACGCTCGGCAAGATCGGCCAG
GATCAGGTGGACGACATGATCGCCCGCAGCGGTGAAGACCGCGCCTTTGTGGAGCGCGCCCTCGCCCCGAATCTCTGA

Upstream 100 bases:

>100_bases
TGCGTCCGTGGGGCATGAAAGAGCTCTACGTGATCGACCCGCACGGCAACCTGTTGAAGCTCGGCGAATACGCCCCCGCC
TGACCGAACCCCGCACCATC

Downstream 100 bases:

>100_bases
TTCTGTGACAAGACGTTTAAGACATAGTGTGAATCACGCTGTGTCTTACGTCCGCAAACCCTCACCGGCATTGGCTTTGC
CGGGGTTTCAAGAGGGGCGA

Product: methionine synthase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 905; Mature: 904

Protein sequence:

>905_residues
MTDHPMRLSGLEPFNIGESTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTY
ARKTEICKRSYDFLVNKVGFAPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDV
VREAIHTVFLYYAIQAGMDMGIVNAGQLGVYENLDPELRDRVEDVVLNRRPDATDRLLEIADRYKGGGTKREENLAWRQE
PVEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRKIAAAGGDVRSRGKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNDILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGAARYVEE
LHADYDRIRTQHANKKATPMVSLAAARANKTKIDWSNYTPPKPKFIGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPD
ILNDEIVGESARRVFSDGKSMLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNIRQQSERPIIDGV
MRPNRCLADFIAPKDTGIADYIGLFAVTGGIGVEKREKQFEADNDDYSAIMLKALADRFAEAFAECLHARVRRDLWGYAP
DETLDNDALIREEYKGIRPAPGYPACPEHTVKRDMFRVLDAQEIGMDLTEALAMTPAASVSGFYLSHPDSTYFTLGKIGQ
DQVDDMIARSGEDRAFVERALAPNL

Sequences:

>Translated_905_residues
MTDHPMRLSGLEPFNIGESTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTY
ARKTEICKRSYDFLVNKVGFAPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDV
VREAIHTVFLYYAIQAGMDMGIVNAGQLGVYENLDPELRDRVEDVVLNRRPDATDRLLEIADRYKGGGTKREENLAWRQE
PVEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRKIAAAGGDVRSRGKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNDILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGAARYVEE
LHADYDRIRTQHANKKATPMVSLAAARANKTKIDWSNYTPPKPKFIGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPD
ILNDEIVGESARRVFSDGKSMLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNIRQQSERPIIDGV
MRPNRCLADFIAPKDTGIADYIGLFAVTGGIGVEKREKQFEADNDDYSAIMLKALADRFAEAFAECLHARVRRDLWGYAP
DETLDNDALIREEYKGIRPAPGYPACPEHTVKRDMFRVLDAQEIGMDLTEALAMTPAASVSGFYLSHPDSTYFTLGKIGQ
DQVDDMIARSGEDRAFVERALAPNL
>Mature_904_residues
TDHPMRLSGLEPFNIGESTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMVR
FLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTYA
RKTEICKRSYDFLVNKVGFAPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDVV
REAIHTVFLYYAIQAGMDMGIVNAGQLGVYENLDPELRDRVEDVVLNRRPDATDRLLEIADRYKGGGTKREENLAWRQEP
VEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLIP
FIEEEKRKIAAAGGDVRSRGKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNDILAKAKVEGADIIGLSGLI
TPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGAARYVEEL
HADYDRIRTQHANKKATPMVSLAAARANKTKIDWSNYTPPKPKFIGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPDI
LNDEIVGESARRVFSDGKSMLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNIRQQSERPIIDGVM
RPNRCLADFIAPKDTGIADYIGLFAVTGGIGVEKREKQFEADNDDYSAIMLKALADRFAEAFAECLHARVRRDLWGYAPD
ETLDNDALIREEYKGIRPAPGYPACPEHTVKRDMFRVLDAQEIGMDLTEALAMTPAASVSGFYLSHPDSTYFTLGKIGQD
QVDDMIARSGEDRAFVERALAPNL

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=917, Percent_Identity=55.5070883315158, Blast_Score=981, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=904, Percent_Identity=63.0530973451327, Blast_Score=1116, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.4956140350877, Blast_Score=949, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 100303; Mature: 100172

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHPMRLSGLEPFNIGESTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENG
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHH
NSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTYARKTEICKRSYDFLVNKVGF
HHHHCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
APEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDV
CCCCEEECCCEEEEEECCHHCCCEEEHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCHH
VREAIHTVFLYYAIQAGMDMGIVNAGQLGVYENLDPELRDRVEDVVLNRRPDATDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
ADRYKGGGTKREENLAWRQEPVEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVI
HHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEE
EGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRKIAAAGGDVRSR
CCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNDILAKAKVEGADIIGLSGL
CCEEEEEECCCHHHHHHHEEEEEEEECCEEEEEEEEEECCHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR
CCCCHHHHHHHHHHHHHHHHEEEEECCEEEECCCHHHEEEEEEECCCCCCCEEECCCCCC
SVSVASSLLSDEGAARYVEELHADYDRIRTQHANKKATPMVSLAAARANKTKIDWSNYTP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEECCCCCCC
PKPKFIGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPDILNDEIVGESARRVFSDGKS
CCHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCCCHHHCHHHHCHHHHHHHHHHHH
MLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNIRQQSERPIIDGV
HHHHHHHCCEEECCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHC
MRPNRCLADFIAPKDTGIADYIGLFAVTGGIGVEKREKQFEADNDDYSAIMLKALADRFA
CCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHH
EAFAECLHARVRRDLWGYAPDETLDNDALIREEYKGIRPAPGYPACPEHTVKRDMFRVLD
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AQEIGMDLTEALAMTPAASVSGFYLSHPDSTYFTLGKIGQDQVDDMIARSGEDRAFVERA
HHHHCCHHHHHHHHCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHH
LAPNL
HCCCC
>Mature Secondary Structure 
TDHPMRLSGLEPFNIGESTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENG
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHH
NSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTYARKTEICKRSYDFLVNKVGF
HHHHCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
APEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDV
CCCCEEECCCEEEEEECCHHCCCEEEHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCHH
VREAIHTVFLYYAIQAGMDMGIVNAGQLGVYENLDPELRDRVEDVVLNRRPDATDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
ADRYKGGGTKREENLAWRQEPVEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVI
HHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEE
EGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRKIAAAGGDVRSR
CCCHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNDILAKAKVEGADIIGLSGL
CCEEEEEECCCHHHHHHHEEEEEEEECCEEEEEEEEEECCHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR
CCCCHHHHHHHHHHHHHHHHEEEEECCEEEECCCHHHEEEEEEECCCCCCCEEECCCCCC
SVSVASSLLSDEGAARYVEELHADYDRIRTQHANKKATPMVSLAAARANKTKIDWSNYTP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEECCCCCCC
PKPKFIGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPDILNDEIVGESARRVFSDGKS
CCHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCCCHHHCHHHHCHHHHHHHHHHHH
MLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNIRQQSERPIIDGV
HHHHHHHCCEEECCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHC
MRPNRCLADFIAPKDTGIADYIGLFAVTGGIGVEKREKQFEADNDDYSAIMLKALADRFA
CCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHH
EAFAECLHARVRRDLWGYAPDETLDNDALIREEYKGIRPAPGYPACPEHTVKRDMFRVLD
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
AQEIGMDLTEALAMTPAASVSGFYLSHPDSTYFTLGKIGQDQVDDMIARSGEDRAFVERA
HHHHCCHHHHHHHHCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHH
LAPNL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]