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The map label for this gene is yieH [H]

Identifier: 187927230

GI number: 187927230

Start: 142031

End: 142720

Strand: Reverse

Name: yieH [H]

Synonym: Rpic_0122

Alternate gene names: 187927230

Gene position: 142720-142031 (Counterclockwise)

Preceding gene: 187927231

Following gene: 187927229

Centisome position: 3.62

GC content: 64.78

Gene sequence:

>690_bases
ATGACTGCAGTGACCTCCTCTGTTTCGAACCACCCGCGCTTTGACGCGATCCTCTTCGATTGCGACGGCGTGCTGGTCGA
CAGCGAACCCATCGTCAACCGTTTGATCTGGGAGATGCTGATCGAGCTTGGCATCGACATCAGCCTGGAAGATTCCATCC
AGCGTTTCCTCGGCAAGGCCATCCGCGAAGAGCTGGATGCGATTGCCGAGATGCGCGGCGCACCGCTGCCGCCGAACTGG
TTGTCCACCTTCCAGGCGCGACGCAACGTGCTGCTCGAAGCCGAAGTGGAAGCCGTGCCGCATATCGGGCACGCCATCGA
TGTGCTCTCCAGGCTCGGCCTGCCGATGGCCGTGGCTTCCGGAGCGGACCGCATGAAGGTGGAGCTGCAACTCAACCGCA
CCGGTCTGATCGAGCATTTCCAGCCGACGGACGTACGCATCTTTTCGGCCACCGAAGTCGCGCGCAGCAAGCCCGCGCCC
GACGTGTATCTGCTCGCCGCCAGCAGCCTCGGGGTATCGCCCTCGCGCTGCCTGGTGATCGAAGACAGCCCCACGGGCGT
TACCGCCGGGCACGCAGCCGGCATGACGGTGCTGGCGTACGCGGGCCGCAATGCACCAGGCCCGCTGATTGCCGCCGGCG
CCACACGCACGTTCACCGATATGCGCGAACTGCCGGAGTTGCTGGCCTGA

Upstream 100 bases:

>100_bases
CCAGATTGAAGCGTTGCTGCGCGACGGACGGATATAAGGCCACATCCACAACGGCGGCAGATGCACGAACCGCCGCCAAC
GAACCAACAACGAAGAGCGC

Downstream 100 bases:

>100_bases
TGCCTGCTGCACAAACCCAGCCCATCGGCCCGGCCGACGCGTGCCCCTGCGGCAACGGCGCCTATGCGCGTTGCTGCGGC
CCGTTCCACGCGGGCGAGGC

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTAVTSSVSNHPRFDAILFDCDGVLVDSEPIVNRLIWEMLIELGIDISLEDSIQRFLGKAIREELDAIAEMRGAPLPPNW
LSTFQARRNVLLEAEVEAVPHIGHAIDVLSRLGLPMAVASGADRMKVELQLNRTGLIEHFQPTDVRIFSATEVARSKPAP
DVYLLAASSLGVSPSRCLVIEDSPTGVTAGHAAGMTVLAYAGRNAPGPLIAAGATRTFTDMRELPELLA

Sequences:

>Translated_229_residues
MTAVTSSVSNHPRFDAILFDCDGVLVDSEPIVNRLIWEMLIELGIDISLEDSIQRFLGKAIREELDAIAEMRGAPLPPNW
LSTFQARRNVLLEAEVEAVPHIGHAIDVLSRLGLPMAVASGADRMKVELQLNRTGLIEHFQPTDVRIFSATEVARSKPAP
DVYLLAASSLGVSPSRCLVIEDSPTGVTAGHAAGMTVLAYAGRNAPGPLIAAGATRTFTDMRELPELLA
>Mature_228_residues
TAVTSSVSNHPRFDAILFDCDGVLVDSEPIVNRLIWEMLIELGIDISLEDSIQRFLGKAIREELDAIAEMRGAPLPPNWL
STFQARRNVLLEAEVEAVPHIGHAIDVLSRLGLPMAVASGADRMKVELQLNRTGLIEHFQPTDVRIFSATEVARSKPAPD
VYLLAASSLGVSPSRCLVIEDSPTGVTAGHAAGMTVLAYAGRNAPGPLIAAGATRTFTDMRELPELLA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=216, Percent_Identity=31.0185185185185, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1789046, Length=189, Percent_Identity=32.8042328042328, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788021, Length=215, Percent_Identity=31.1627906976744, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87082080, Length=207, Percent_Identity=31.8840579710145, Blast_Score=61, Evalue=7e-11,
Organism=Drosophila melanogaster, GI45550911, Length=209, Percent_Identity=30.1435406698565, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20129151, Length=219, Percent_Identity=28.7671232876712, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116008157, Length=193, Percent_Identity=32.6424870466321, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17137324, Length=190, Percent_Identity=32.6315789473684, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24647; Mature: 24515

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVTSSVSNHPRFDAILFDCDGVLVDSEPIVNRLIWEMLIELGIDISLEDSIQRFLGKA
CCCCCCCCCCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IREELDAIAEMRGAPLPPNWLSTFQARRNVLLEAEVEAVPHIGHAIDVLSRLGLPMAVAS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEEHHHHCCCCHHHHHHHHHCCCCEEECC
GADRMKVELQLNRTGLIEHFQPTDVRIFSATEVARSKPAPDVYLLAASSLGVSPSRCLVI
CCCEEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHCCCCCCEEEEEECCCCCCCCEEEEE
EDSPTGVTAGHAAGMTVLAYAGRNAPGPLIAAGATRTFTDMRELPELLA
ECCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
TAVTSSVSNHPRFDAILFDCDGVLVDSEPIVNRLIWEMLIELGIDISLEDSIQRFLGKA
CCCCCCCCCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IREELDAIAEMRGAPLPPNWLSTFQARRNVLLEAEVEAVPHIGHAIDVLSRLGLPMAVAS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEEHHHHCCCCHHHHHHHHHCCCCEEECC
GADRMKVELQLNRTGLIEHFQPTDVRIFSATEVARSKPAPDVYLLAASSLGVSPSRCLVI
CCCEEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHCCCCCCEEEEEECCCCCCCCEEEEE
EDSPTGVTAGHAAGMTVLAYAGRNAPGPLIAAGATRTFTDMRELPELLA
ECCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]