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The map label for this gene is yfcG [H]

Identifier: 187927188

GI number: 187927188

Start: 90003

End: 90710

Strand: Reverse

Name: yfcG [H]

Synonym: Rpic_0078

Alternate gene names: 187927188

Gene position: 90710-90003 (Counterclockwise)

Preceding gene: 187927189

Following gene: 187927187

Centisome position: 2.3

GC content: 65.68

Gene sequence:

>708_bases
ATGATCGACCTCTACTACTGCGCCACGCCCAATGGCCTGAAGATGGCACTGTTCTTTGCCGAAACCGGCTTGCCGCACCG
CGTCATCCCCGTCGATATCAGCCGGGGTGACCAGTTCAAGCCCGAGTTCCTGGCCATCTCGCCCAACAACAAGATCCCGG
CCATGGTCGATCACGACCCGGCCGAAGGCAGCGACCCCGTGGTGCTGTTTGAATCGGGCGCCATGCTGCTTTACCTGGCC
GAGAAGACCGGCCAGCTGACGCCCGCCGACCTGCACGGCCGCATGGAAGTGCTCAAGTGGCTGTTCTGGCAGGTGGGCGG
CCTGGGCCCGATGGCCGGGCAGATCGGCCACTTCAACGTGTATGCCCCCGAGCGCGTGCACTACGCCATCGAGCGCTACA
CCAAGGAAACGAACCGCCTGTACGGTGTGCTCGACCGCCGCTTGGCCGATCGCCCCTACATTGCCGGCAACGACTACACC
ATCGCCGACATCGCGGCCTATCCGTGGATCGTGCCGCACGCCGGGCATGGTCAGGACCTGAACGACTTTCCGAATCTGCA
GCGCTGGTTCGAAGCCGTTGGCGCACGCCCTGCCACGCAGCGCGCCTATGCCGGGGTCGAGCGCGCCTACTCGCGCCGCC
GCGAAGACATCTCCGACGACGAGCGCAACGTGCTGTTGGGCCAGACGGCCAGCTCCACCGCGCGCTGA

Upstream 100 bases:

>100_bases
CCGTAAGTGGTTACCTGAAAATTCCAGTTTCTGGACATTACAAAAGGCCACTCGCGCGCCTACGCTGTCAACCAACATGA
CAGAACACGAGGGCCTCGCC

Downstream 100 bases:

>100_bases
TCCTCGCGGAGCCTCTGCGCTCCGCCCCTCTCTCCGCTTTCCTCTTCTGACAGGAGCCGACCATGCCGGCATCGCTGTGG
TGGTTGTGCCTGGGCGCGTT

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MIDLYYCATPNGLKMALFFAETGLPHRVIPVDISRGDQFKPEFLAISPNNKIPAMVDHDPAEGSDPVVLFESGAMLLYLA
EKTGQLTPADLHGRMEVLKWLFWQVGGLGPMAGQIGHFNVYAPERVHYAIERYTKETNRLYGVLDRRLADRPYIAGNDYT
IADIAAYPWIVPHAGHGQDLNDFPNLQRWFEAVGARPATQRAYAGVERAYSRRREDISDDERNVLLGQTASSTAR

Sequences:

>Translated_235_residues
MIDLYYCATPNGLKMALFFAETGLPHRVIPVDISRGDQFKPEFLAISPNNKIPAMVDHDPAEGSDPVVLFESGAMLLYLA
EKTGQLTPADLHGRMEVLKWLFWQVGGLGPMAGQIGHFNVYAPERVHYAIERYTKETNRLYGVLDRRLADRPYIAGNDYT
IADIAAYPWIVPHAGHGQDLNDFPNLQRWFEAVGARPATQRAYAGVERAYSRRREDISDDERNVLLGQTASSTAR
>Mature_235_residues
MIDLYYCATPNGLKMALFFAETGLPHRVIPVDISRGDQFKPEFLAISPNNKIPAMVDHDPAEGSDPVVLFESGAMLLYLA
EKTGQLTPADLHGRMEVLKWLFWQVGGLGPMAGQIGHFNVYAPERVHYAIERYTKETNRLYGVLDRRLADRPYIAGNDYT
IADIAAYPWIVPHAGHGQDLNDFPNLQRWFEAVGARPATQRAYAGVERAYSRRREDISDDERNVLLGQTASSTAR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI4504187, Length=191, Percent_Identity=29.8429319371728, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI124249394, Length=191, Percent_Identity=29.8429319371728, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788640, Length=202, Percent_Identity=55.4455445544555, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI87082195, Length=241, Percent_Identity=39.8340248962656, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI87081789, Length=167, Percent_Identity=33.5329341317365, Blast_Score=77, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324100, Length=238, Percent_Identity=31.9327731092437, Blast_Score=126, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28571670, Length=197, Percent_Identity=26.3959390862944, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17864598, Length=213, Percent_Identity=28.6384976525822, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17933730, Length=195, Percent_Identity=27.1794871794872, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17864594, Length=203, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922160, Length=184, Percent_Identity=27.7173913043478, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24646249, Length=175, Percent_Identity=29.1428571428571, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17864592, Length=196, Percent_Identity=26.530612244898, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646251, Length=212, Percent_Identity=26.8867924528302, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45549270, Length=211, Percent_Identity=26.0663507109005, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI85725204, Length=216, Percent_Identity=26.3888888888889, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17737923, Length=216, Percent_Identity=26.3888888888889, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26364; Mature: 26364

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLYYCATPNGLKMALFFAETGLPHRVIPVDISRGDQFKPEFLAISPNNKIPAMVDHDP
CEEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCEEECCCC
AEGSDPVVLFESGAMLLYLAEKTGQLTPADLHGRMEVLKWLFWQVGGLGPMAGQIGHFNV
CCCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
YAPERVHYAIERYTKETNRLYGVLDRRLADRPYIAGNDYTIADIAAYPWIVPHAGHGQDL
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEHHHHCCEECCCCCCCCCC
NDFPNLQRWFEAVGARPATQRAYAGVERAYSRRREDISDDERNVLLGQTASSTAR
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MIDLYYCATPNGLKMALFFAETGLPHRVIPVDISRGDQFKPEFLAISPNNKIPAMVDHDP
CEEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCEEECCCC
AEGSDPVVLFESGAMLLYLAEKTGQLTPADLHGRMEVLKWLFWQVGGLGPMAGQIGHFNV
CCCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
YAPERVHYAIERYTKETNRLYGVLDRRLADRPYIAGNDYTIADIAAYPWIVPHAGHGQDL
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEHHHHCCEECCCCCCCCCC
NDFPNLQRWFEAVGARPATQRAYAGVERAYSRRREDISDDERNVLLGQTASSTAR
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]